The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin.


Abstract

Differential scanning calorimetry has been used to study the thermal unfolding of the 255-316 C-terminal fragment of thermolysin. The concentration effect on the calorimetric transitions of the fragment in 0.1 M NaCl and 20 mM phosphate buffer, pH 7.5, shows that it behaves as a highly stable dimer in solution, within the concentration range 0.19-4.55 mg/ml, undergoing a reversible two-state thermal unfolding process. The thermodynamic parameters of unfolding (delta G = 60 +/- 6 kJ/(mol of dimer) at 20 degrees C) are similar to those normally observed for small, compact, globular proteins. This and previous studies [1989, Eur. J. Biochem. 180, 513-518] show that the 255-316 fragment folds into a stable, native-like globular structure. Study holds ProTherm entries: 10548, 10549, 10550, 10551, 10552, 10553, 10554, 10555 Extra Details: 255-316 C-terminal fragment differential scanning calorimetry; thermolysin; protein stability;,protein domain; domain folding

Submission Details

ID: WNzjPM7h3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:40 p.m.

Version: 1

Publication Details
Conejero-Lara F;De Filippis V;Fontana A;Mateo PL,FEBS Lett. (1994) The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin. PMID:8187875
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1TRL 1995-02-07 NMR SOLUTION STRUCTURE OF THE C-TERMINAL FRAGMENT 255-316 OF THERMOLYSIN: A DIMER FORMED BY SUBUNITS HAVING THE NATIVE STRUCTURE
5JVI 2016-12-21 1.12 Thermolysin in complex with JC148.
5N3V 2017-06-21 1.12 Thermolysin in complex with inhibitor JC292
4N4E 2014-04-02 1.13 Thermolysin in complex with UBTLN58
6GHX 2019-02-06 1.16 Alzheimer's Amyloid-Beta Peptide Fragment 31-35 in Complex with Cd-substituted Thermolysin
5JS3 2016-12-21 1.16 Thermolysin in complex with JC114.
4MZN 2014-04-02 1.17 Thermolysin in complex with UBTLN59
5ONQ 2018-08-29 1.17 Alzheimer's Amyloid-Beta Peptide Fragment 29-40 in Complex with Cd-substituted Thermolysin
5JSS 2016-12-21 1.19 Thermolysin in complex with JC149.
5FSJ 2016-10-26 1.2 Structure of thermolysin prepared by the 'soak-and-freeze' method under 45 bar of oxygen pressure
3DNZ 2009-07-07 1.2 Thermolysin by LB nanotemplate method before high X-Ray dose on ESRF ID14-2 beamline
5M9W 2017-08-16 1.21 Experimental MAD phased structure of thermolysin in complex with inhibitor JC65.
5N2X 2017-06-21 1.21 Thermolysin in complex with inhibitor JC272
3DO2 2009-07-07 1.22 Thermolysin by LB nanotemplate method after high X-Ray dose on ESRF ID14-2 beamline
5N34 2017-06-21 1.22 Thermolysin in complex with inhibitor JC276
4MWP 2014-04-02 1.23 Thermolysin in complex with UBTLN46
5L3U 2016-12-21 1.23 Thermolysin in complex with JC149 (MPD cryo protectant)
5LWD 2016-12-21 1.23 Thermolysin in complex with inhibitor (JC96)
4N5P 2014-04-02 1.25 Thermolysin in complex with UBTLN20
5MNR 2017-06-21 1.25 Thermolysin in complex with inhibitor JC256
5L41 2016-12-21 1.25 Thermolysin in complex with JC148 (MPD cryo protectant)
5LVD 2017-08-16 1.25 Thermolysin in complex with inhibitor (JC67)
5JT9 2016-12-21 1.26 Thermolysin in complex with JC106.
5A3Y 2015-11-11 1.27 SAD structure of Thermolysin obtained by multi crystal data collection
3T87 2012-08-01 1.28 Thermolysin In Complex With UBTLN28
3T74 2012-08-01 1.28 Thermolysin In Complex With UBTLN27
5L8P 2016-12-21 1.29 Thermolysin in complex with JC114 (PEG400 cryo protectant)
4OI5 2014-04-02 1.3 Glycerol-free structure of thermolysin in complex with ubtln58
5MA7 2017-08-16 1.3 Structure of thermolysin in complex with inhibitor (JC306).
5LIF 2016-12-21 1.31 Thermolysin in complex with inhibitor
4MTW 2014-04-02 1.32 Thermolysin in complex with UBTLN36
5M5F 2017-08-16 1.33 Thermolysin in complex with inhibitor and krypton
3FGD 2009-12-15 1.33 Drugscore FP: Thermoylsin in complex with fragment.
3DO1 2009-07-07 1.33 Thermolysin by Classical hanging drop method before high X-Ray dose on ESRF ID14-2 beamline
5ONP 2018-08-29 1.34 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Cd-substituted Thermolysin
5N3Y 2017-06-21 1.34 Thermolysin in complex with inhibitor JC267
5DPE 2015-10-28 1.34 Thermolysin in complex with inhibitor.
4MXJ 2014-04-02 1.35 Thermolysin in complex with UBTLN35
3DO0 2009-07-07 1.36 Thermolysin by classical hanging drop method after high X-Ray dose on esrf ID14-2 beamline
5N31 2017-06-21 1.37 Thermolysin in complex with inhibitor JC277
5N2Z 2017-06-21 1.37 Thermolysin in complex with inhibitor JC286
5JXN 2016-12-21 1.38 Thermolysin in complex with JC240.
4D9W 2013-01-16 1.38 Thermolysin In Complex With UBTLN32
5N2T 2017-06-21 1.38 Thermolysin in complex with inhibitor JC287
6FSM 2018-05-02 1.39 Crystal structure of TCE-treated Thermolysin
5ONR 2018-08-29 1.39 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Thermolysin
3T8D 2012-08-01 1.41 Thermolysin In Complex With UBTLN31
3FVP 2010-02-09 1.41 Thermolysin inhibition
6FJ2 2018-01-31 1.43 Structure of Thermolysin solved from SAD data collected at the peak of the Zn absorption edge on ID30B
4N66 2014-04-02 1.44 Thermolysin in complex with UBTLN37
3T8F 2012-08-01 1.44 Thermolysin In Complex With UBTLN34
5M69 2017-08-16 1.44 Thermolysin in complex with inhibitor and xenon
5FXN 2016-03-16 1.45 Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the ESRF RoboDiff goniometer
3QGO 2012-01-04 1.45 Structure of Thermolysin in complex with L-Phenylalanine methylester
3T8H 2012-08-01 1.45 Thermolysin In Complex With UBTLN29
5DPF 2015-10-28 1.47 Thermolysin in complex with inhibitor.
3QH5 2012-01-04 1.5 Structure of Thermolysin in complex with N-Carbobenzyloxy-L-aspartic acid and L-Phenylalanine Methyl Ester
3T8G 2012-08-01 1.5 Thermolysin In Complex With UBTLN26
5FSS 2016-10-26 1.5 Structure of thermolysin prepared by the 'soak-and-freeze' method under 40 bar of krypton pressure
6QF3 2019-07-10 1.52 X-Ray structure of Thermolysin soaked with sodium aspartate on a silicon chip
3MS3 2011-05-11 1.54 Crystal structure of Thermolysin in complex with Aniline
3QH1 2012-01-04 1.55 Structure of Thermolysin in complex with N-benzyloxycarbonyl-L-aspartic acid
3FXS 2010-02-09 1.55 Metal exchange in thermolysin
3FV4 2010-02-09 1.56 Thermolysin inhibition
4H57 2012-10-03 1.56 Thermolysin inhibition
6TMN 1989-01-09 1.6 Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond
3FB0 2009-12-08 1.6 Metal exchange in thermolysin
8TLN 1994-04-30 1.6 STRUCTURAL COMPARISON SUGGESTS THAT THERMOLYSIN AND RELATED NEUTRAL PROTEASES UNDERGO HINGE-BENDING MOTION DURING CATALYSIS
5UN3 2017-02-22 1.6 Tetragonal thermolysin (295 K) in the presence of 50% xylose
5UU9 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 40% xylose as cryoprotectant
2TMN 1989-01-09 1.6 CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN
3T73 2012-08-01 1.6 Thermolysin In Complex With UBTLN22
5UUE 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
5UUC 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% mpd as cryoprotectant
5TMN 1989-01-09 1.6 Slow-and fast-binding inhibitors of thermolysin display different modes of binding. crystallographic analysis of extended phosphonamidate transition-state analogues
1KJP 2002-12-05 1.6 Thermolysin complexed with Z-L-Glutamic acid (benzyloxycarbonyl-L-Glutamic acid)
5UUA 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
5UUD 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% dmf as cryoprotectant
5UU7 2017-03-08 1.6 Tetragonal thermolysin (295 K) in the presence of 50% mpd
1KKK 2002-12-10 1.6 Thermolysin complexed with Z-L-Aspartic Acid (benzyloxycarbonyl-L-Aspartic Acid)
5UUB 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 25% xylose/25% mpd as cryoprotectant
1KEI 2002-11-16 1.6 Thermolysin (substrate-free)
1KJO 2002-12-05 1.6 Thermolysin complexed with Z-L-Threonine (benzyloxycarbonyl-L-Threonine)
2TLX 2000-03-13 1.65 THERMOLYSIN (NATIVE)
3MSA 2011-05-11 1.66 Crystal structure of Thermolysin in complex with 3-Bromophenol
3T8C 2012-08-01 1.66 Thermolysin In Complex With UBTLN30
3F28 2009-11-17 1.68 Thermolysin inhibition
1THL 1994-01-31 1.7 Thermolysin complexed with a novel glutaramide derivative, n-(1-(2(r,s)-carboxy-4-phenylbutyl) cyclopentylcarbonyl)-(s)-tryptophan
1LNF 1995-05-08 1.7 A structural analysis of metal substitutions in thermolysin
1LNE 1995-05-08 1.7 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
3TMN 1989-01-09 1.7 THE BINDING OF L-VALYL-L-TRYPTOPHAN TO CRYSTALLINE THERMOLYSIN ILLUSTRATES THE MODE OF INTERACTION OF A PRODUCT OF PEPTIDE HYDROLYSIS
4TMN 1989-01-09 1.7 SLOW-AND FAST-BINDING INHIBITORS OF THERMOLYSIN DISPLAY DIFFERENT MODES OF BINDING. CRYSTALLOGRAPHIC ANALYSIS OF EXTENDED PHOSPHONAMIDATE TRANSITION-STATE ANALOGUES
1FJQ 2001-04-18 1.7 THERMOLYSIN (70% ACETONE SOAKED CRYSTALS)
1PE5 2004-06-08 1.7 Thermolysin with tricyclic inhibitor
1HYT 1994-07-31 1.7 RE-DETERMINATION AND REFINEMENT OF THE COMPLEX OF BENZYLSUCCINIC ACID WITH THERMOLYSIN AND ITS RELATION TO THE COMPLEX WITH CARBOXYPEPTIDASE A
6FHP 2018-09-12 1.7 DAIP in complex with a C-terminal fragment of thermolysin
1LND 1995-05-08 1.7 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1KS7 2003-01-11 1.7 Thermolysin complexed with Z-D-Aspartic acid (benzyloxycarbonyl-D-Aspartic acid)
1ZDP 2005-04-26 1.7 Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (S)-thiorphan
5FSP 2016-10-26 1.7 Structure of thermolysin prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure
1KRO 2003-01-10 1.7 Thermolysin complexed with Z-D-Threonine (benzyloxycarbonyl-D-Threonine)
1Z9G 2005-04-19 1.7 Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (R)-retro-thiorphan
6QF2 2019-07-10 1.73 X-Ray structure of Thermolysin crystallized on a silicon chip
3FCQ 2009-12-08 1.75 Thermolysin inhibition
2WHZ 2010-05-12 1.75 Dipeptide Inhibitors of Thermolysin
1KL6 2002-12-11 1.8 Thermolysin complexed with Z-L-Alanine (benzyloxycarbonyl-L-Alanine)
1LNB 1995-05-08 1.8 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1PE8 2004-06-08 1.8 Thermolysin with monocyclic inhibitor
1KR6 2003-01-09 1.8 Thermolysin complexed with Z-D-Glutamic acid (benzyloxycarbonyl-D-Glutamic acid)
1LNC 1995-05-08 1.8 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
4TNL 2014-07-09 1.8 1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL
3SSB 2011-09-07 1.8 Structure of Insect Metalloproteinase Inhibitor in Complex with Thermolysin
6IG7 2018-11-07 1.8 Crystal structure of thermolysin delivered in polyacrylamide using x-ray free electron laser
1PE7 2004-06-08 1.82 Thermolysin with bicyclic inhibitor
2A7G 2005-07-19 1.85 On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength
3N21 2011-05-25 1.87 Crystal structure of Thermolysin in complex with S-1,2-Propandiol
3EIM 2009-10-06 1.88 Metal exchange in Thermolysin
5O8N 2017-08-09 1.9 Structure of thermolysin at room temperature via a method of acoustically induced rotation.
1TMN 1989-01-09 1.9 Binding of n-carboxymethyl dipeptide inhibitors to thermolysin determined by x-ray crystallography. a novel class of transition-state analogues for zinc peptidases
4D91 2013-01-16 1.9 Thermolysin In Complex With DMSO And Acetate
1LNA 1995-05-08 1.9 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
5WR5 2017-08-16 1.9 Thermolysin, liganded form with cryo condition 1
1FJW 2001-04-18 1.9 THERMOLYSIN (50 MM PHENOL SOAKED)
1KTO 2003-01-17 1.9 Thermolysin complexed with Z-D-Alanine (benzyloxycarbonyl-D-Alanine)
3FBO 2009-12-08 1.92 Metal exchange in Thermolysin
3FOR 2010-01-12 1.93 Thermolysin Complexed with BNPA (2-Benzyl-3-Nitro Propanoic Acid Amide)
3F2P 2009-11-17 1.95 Thermolysin inhibition
4TLI 2000-03-13 1.95 THERMOLYSIN (25% ISOPROPANOL SOAKED CRYSTALS)
7TLI 2000-03-13 1.95 THERMOLYSIN (90% ISOPROPANOL SOAKED CRYSTALS)
2WI0 2010-05-12 1.95 Dipeptide Inhibitors of Thermolysin
3TLI 2000-02-18 1.95 THERMOLYSIN (10% ISOPROPANOL SOAKED CRYSTALS)
2TLI 2000-02-18 1.95 THERMOLYSIN (5% ISOPROPANOL SOAKED CRYSTALS)
3T2H 2011-12-28 1.95 Tetragonal thermolysin in the presence of TMAO
3MSN 2011-05-11 1.97 Crystal structure of Thermolysin in complex with N-methylurea
3FLF 2010-01-12 1.97 Thermolysin inhibition
2G4Z 2007-02-20 1.98 anomalous substructure of thermolysin
3LS7 2011-02-23 1.98 Crystal structure of Thermolysin in complex with Xenon
6D5U 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
1FJV 2001-04-18 2.0 THERMOLYSIN (60% ACETONITRILE SOAKED CRYSTALS)
1FJO 2001-04-18 2.0 THERMOLYSIN (60% ACETONE SOAKED CRYSTALS)
5WR2 2017-08-16 2.0 Thermolysin, SFX liganded form with oil-based carrier
6D5R 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
6D5N 2018-10-31 2.0 Hexagonal thermolysin (295) in the presence of 50% xylose
1QF1 1999-12-29 2.0 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYLHEPTANOYL)-PHE-ALA. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
1FJU 2001-04-18 2.0 THERMOLYSIN (80% ACETONITRILE SOAKED CRYSTALS)
5TAK 2017-08-30 2.0 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
6D5O 2018-09-19 2.0 Hexagonal thermolysin (295 K) in the presence of 50% DMF
6D5S 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
6D5Q 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
1FJ3 2001-04-18 2.0 THERMOLYSIN (50% ACETONE SOAKED)
3ZI6 2013-05-01 2.0 Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the GRob robot goniometer
3T2J 2011-12-28 2.0 Tetragonal thermolysin in the presence of betaine
6D5T 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
5TAJ 2017-08-30 2.03 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5TAC 2017-08-30 2.04 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
3FXP 2010-02-09 2.05 Thermolysin inhibition
3NN7 2011-05-25 2.05 Crystal structure of Thermolysin in complex with 2-bromoacetate
1TLI 2000-02-18 2.05 THERMOLYSIN (2% ISOPROPANOL SOAKED CRYSTALS)
1QF2 1999-12-29 2.06 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-GLY-(5-PHENYLPROLINE). PARAMETERS FOR ZN-MONODENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
3MSF 2011-05-11 2.09 Crystal structure of Thermolysin in complex with Urea
5T9I 2017-08-30 2.09 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5TAD 2017-08-30 2.09 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5WR4 2017-08-16 2.1 Thermolysin, SFX unliganded form with oil-based carrier
3T2I 2011-12-28 2.1 Tetragonal thermolysin in the presence of sarcosine
5TLI 2000-03-13 2.1 THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
1OS0 2003-03-25 2.1 Thermolysin with an alpha-amino phosphinic inhibitor
4OW3 2014-03-12 2.1 Thermolysin structure determined by free-electron laser
1TLX 2000-03-13 2.1 THERMOLYSIN (NATIVE)
5T9K 2017-08-30 2.1 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
6TLI 2000-03-13 2.1 THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
5WR3 2017-08-16 2.1 Thermolysin, SFX liganded form with water-based carrier
5T9Q 2017-08-30 2.1 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
1Y3G 2006-01-17 2.1 Crystal Structure of a Silanediol Protease Inhibitor Bound to Thermolysin
1GXW 2002-12-05 2.18 the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate
1QF0 1999-12-29 2.2 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-PHE-TYR. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
3P7V 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure C (4.8 MGy)
1FJT 2001-04-18 2.2 THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)
3P7T 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure A (0.1 MGy)
3P7S 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure D (7.2 MGy)
3P7U 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure B (2.4 MGy)
6SEL 2019-08-21 2.2 Multicrystal structure of Thermolysin at room temperature using a multilayer monochromator.
3P7P 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure A (0.1 MGy)
8TLI 2000-03-13 2.2 THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)
3P7W 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure D (7.1 MGy)
3P7Q 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure B (2.5 MGy)
3P7R 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure C (4.9 MGy)
5DLH 2015-11-18 2.25 SFX structure of thermolysin
4TLN 1982-05-26 2.3 BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS
5TAE 2017-08-30 2.3 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
1TLP 1989-01-09 2.3 CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN
7TLN 1983-03-09 2.3 STRUCTURAL ANALYSIS OF THE INHIBITION OF THERMOLYSIN BY AN ACTIVE-SITE-DIRECTED IRREVERSIBLE INHIBITOR
1L3F 2002-07-03 2.3 Thermolysin in the Absence of Substrate has an Open Conformation
5TLN 1982-05-26 2.3 BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS
5TAI 2017-08-30 2.3 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5WR6 2017-08-16 2.3 Thermolysin, liganded form with cryo condition 2
5K7T 2017-04-05 2.5 MicroED structure of thermolysin at 2.5 A resolution
5UU8 2017-03-08 2.5 Tetragonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
6D5P 2018-09-19 3.0 Hexagonal thermolysin cryocooled to 100 K with 20% xylose as cryoprotectant

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.4 Thermolysin P43133 THER_GEOSE
100.0 Thermolysin P00800 THER_BACTH