The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin.
Abstract
Differential scanning calorimetry has been used to study the thermal unfolding of the 255-316 C-terminal fragment of thermolysin. The concentration effect on the calorimetric transitions of the fragment in 0.1 M NaCl and 20 mM phosphate buffer, pH 7.5, shows that it behaves as a highly stable dimer in solution, within the concentration range 0.19-4.55 mg/ml, undergoing a reversible two-state thermal unfolding process. The thermodynamic parameters of unfolding (delta G = 60 +/- 6 kJ/(mol of dimer) at 20 degrees C) are similar to those normally observed for small, compact, globular proteins. This and previous studies [1989, Eur. J. Biochem. 180, 513-518] show that the 255-316 fragment folds into a stable, native-like globular structure. Study holds ProTherm entries: 10548, 10549, 10550, 10551, 10552, 10553, 10554, 10555 Extra Details: 255-316 C-terminal fragment differential scanning calorimetry; thermolysin; protein stability;,protein domain; domain folding
Submission Details
ID: WNzjPM7h3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:40 p.m.
Version: 1
Publication Details
Conejero-Lara F;De Filippis V;Fontana A;Mateo PL,FEBS Lett. (1994) The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin. PMID:8187875Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 5WR2 | 2016-11-29T00:00:00+0000 | 2.0 | Thermolysin, SFX liganded form with oil-based carrier |
| 5WR5 | 2016-11-29T00:00:00+0000 | 1.9 | Thermolysin, liganded form with cryo condition 1 |
| 5WR6 | 2016-11-29T00:00:00+0000 | 2.3 | Thermolysin, liganded form with cryo condition 2 |
| 5WR3 | 2016-11-29T00:00:00+0000 | 2.1 | Thermolysin, SFX liganded form with water-based carrier |
| 5WR4 | 2016-11-29T00:00:00+0000 | 2.1 | Thermolysin, SFX unliganded form with oil-based carrier |
| 6GHX | 2018-05-09T00:00:00+0000 | 1.16 | Alzheimer's Amyloid-Beta Peptide Fragment 31-35 in Complex with Cd-substituted Thermolysin |
| 5DLH | 2015-09-04T00:00:00+0000 | 2.25 | SFX structure of thermolysin |
| 6FHP | 2018-01-15T00:00:00+0000 | 1.7 | DAIP in complex with a C-terminal fragment of thermolysin |
| 6FSM | 2018-02-19T00:00:00+0000 | 1.39 | Crystal structure of TCE-treated Thermolysin |
| 5ONP | 2017-08-04T00:00:00+0000 | 1.34 | Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Cd-substituted Thermolysin |