The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin.
Abstract
Differential scanning calorimetry has been used to study the thermal unfolding of the 255-316 C-terminal fragment of thermolysin. The concentration effect on the calorimetric transitions of the fragment in 0.1 M NaCl and 20 mM phosphate buffer, pH 7.5, shows that it behaves as a highly stable dimer in solution, within the concentration range 0.19-4.55 mg/ml, undergoing a reversible two-state thermal unfolding process. The thermodynamic parameters of unfolding (delta G = 60 +/- 6 kJ/(mol of dimer) at 20 degrees C) are similar to those normally observed for small, compact, globular proteins. This and previous studies [1989, Eur. J. Biochem. 180, 513-518] show that the 255-316 fragment folds into a stable, native-like globular structure. Study holds ProTherm entries: 10548, 10549, 10550, 10551, 10552, 10553, 10554, 10555 Extra Details: 255-316 C-terminal fragment differential scanning calorimetry; thermolysin; protein stability;,protein domain; domain folding
Submission Details
ID: WNzjPM7h3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:40 p.m.
Version: 1
Publication Details
Conejero-Lara F;De Filippis V;Fontana A;Mateo PL,FEBS Lett. (1994) The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin. PMID:8187875Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 1TRL | 1995-02-07 | NMR SOLUTION STRUCTURE OF THE C-TERMINAL FRAGMENT 255-316 OF THERMOLYSIN: A DIMER FORMED BY SUBUNITS HAVING THE NATIVE STRUCTURE | |
| 5JVI | 2016-12-21 | 1.12 | Thermolysin in complex with JC148. |
| 5N3V | 2017-06-21 | 1.12 | Thermolysin in complex with inhibitor JC292 |
| 4N4E | 2014-04-02 | 1.13 | Thermolysin in complex with UBTLN58 |
| 6GHX | 2019-02-06 | 1.16 | Alzheimer's Amyloid-Beta Peptide Fragment 31-35 in Complex with Cd-substituted Thermolysin |
| 5JS3 | 2016-12-21 | 1.16 | Thermolysin in complex with JC114. |
| 5ONQ | 2018-08-29 | 1.17 | Alzheimer's Amyloid-Beta Peptide Fragment 29-40 in Complex with Cd-substituted Thermolysin |
| 4MZN | 2014-04-02 | 1.17 | Thermolysin in complex with UBTLN59 |
| 5JSS | 2016-12-21 | 1.19 | Thermolysin in complex with JC149. |
| 3DNZ | 2009-07-07 | 1.2 | Thermolysin by LB nanotemplate method before high X-Ray dose on ESRF ID14-2 beamline |
| 5FSJ | 2016-10-26 | 1.2 | Structure of thermolysin prepared by the 'soak-and-freeze' method under 45 bar of oxygen pressure |
| 5M9W | 2017-08-16 | 1.21 | Experimental MAD phased structure of thermolysin in complex with inhibitor JC65. |
| 5N2X | 2017-06-21 | 1.21 | Thermolysin in complex with inhibitor JC272 |
| 5N34 | 2017-06-21 | 1.22 | Thermolysin in complex with inhibitor JC276 |
| 3DO2 | 2009-07-07 | 1.22 | Thermolysin by LB nanotemplate method after high X-Ray dose on ESRF ID14-2 beamline |
| 4MWP | 2014-04-02 | 1.23 | Thermolysin in complex with UBTLN46 |
| 5L3U | 2016-12-21 | 1.23 | Thermolysin in complex with JC149 (MPD cryo protectant) |
| 5LWD | 2016-12-21 | 1.23 | Thermolysin in complex with inhibitor (JC96) |
| 4N5P | 2014-04-02 | 1.25 | Thermolysin in complex with UBTLN20 |
| 5MNR | 2017-06-21 | 1.25 | Thermolysin in complex with inhibitor JC256 |
| 5L41 | 2016-12-21 | 1.25 | Thermolysin in complex with JC148 (MPD cryo protectant) |
| 5LVD | 2017-08-16 | 1.25 | Thermolysin in complex with inhibitor (JC67) |
| 5JT9 | 2016-12-21 | 1.26 | Thermolysin in complex with JC106. |
| 5A3Y | 2015-11-11 | 1.27 | SAD structure of Thermolysin obtained by multi crystal data collection |
| 3T74 | 2012-08-01 | 1.28 | Thermolysin In Complex With UBTLN27 |
| 3T87 | 2012-08-01 | 1.28 | Thermolysin In Complex With UBTLN28 |
| 5L8P | 2016-12-21 | 1.29 | Thermolysin in complex with JC114 (PEG400 cryo protectant) |
| 4OI5 | 2014-04-02 | 1.3 | Glycerol-free structure of thermolysin in complex with ubtln58 |
| 5MA7 | 2017-08-16 | 1.3 | Structure of thermolysin in complex with inhibitor (JC306). |
| 5LIF | 2016-12-21 | 1.31 | Thermolysin in complex with inhibitor |
| 4MTW | 2014-04-02 | 1.32 | Thermolysin in complex with UBTLN36 |
| 3FGD | 2009-12-15 | 1.33 | Drugscore FP: Thermoylsin in complex with fragment. |
| 3DO1 | 2009-07-07 | 1.33 | Thermolysin by Classical hanging drop method before high X-Ray dose on ESRF ID14-2 beamline |
| 5M5F | 2017-08-16 | 1.33 | Thermolysin in complex with inhibitor and krypton |
| 5N3Y | 2017-06-21 | 1.34 | Thermolysin in complex with inhibitor JC267 |
| 5DPE | 2015-10-28 | 1.34 | Thermolysin in complex with inhibitor. |
| 5ONP | 2018-08-29 | 1.34 | Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Cd-substituted Thermolysin |
| 4MXJ | 2014-04-02 | 1.35 | Thermolysin in complex with UBTLN35 |
| 3DO0 | 2009-07-07 | 1.36 | Thermolysin by classical hanging drop method after high X-Ray dose on esrf ID14-2 beamline |
| 5N31 | 2017-06-21 | 1.37 | Thermolysin in complex with inhibitor JC277 |
| 5N2Z | 2017-06-21 | 1.37 | Thermolysin in complex with inhibitor JC286 |
| 4D9W | 2013-01-16 | 1.38 | Thermolysin In Complex With UBTLN32 |
| 5N2T | 2017-06-21 | 1.38 | Thermolysin in complex with inhibitor JC287 |
| 5JXN | 2016-12-21 | 1.38 | Thermolysin in complex with JC240. |
| 6FSM | 2018-05-02 | 1.39 | Crystal structure of TCE-treated Thermolysin |
| 5ONR | 2018-08-29 | 1.39 | Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Thermolysin |
| 6N4W | 2019-10-02 | 1.4 | Tetragonal thermolysin (with 50% xylose) cryocooled in a nitrogen gas stream to 100 K |
| 6N4Z | 2019-10-02 | 1.4 | Tetragonal thermolysin (with 50% xylose) plunge cooled in liquid nitrogen to 77 K |
| 3T8D | 2012-08-01 | 1.41 | Thermolysin In Complex With UBTLN31 |
| 3FVP | 2010-02-09 | 1.41 | Thermolysin inhibition |
| 6FJ2 | 2018-01-31 | 1.43 | Structure of Thermolysin solved from SAD data collected at the peak of the Zn absorption edge on ID30B |
| 5M69 | 2017-08-16 | 1.44 | Thermolysin in complex with inhibitor and xenon |
| 4N66 | 2014-04-02 | 1.44 | Thermolysin in complex with UBTLN37 |
| 3T8F | 2012-08-01 | 1.44 | Thermolysin In Complex With UBTLN34 |
| 3QGO | 2012-01-04 | 1.45 | Structure of Thermolysin in complex with L-Phenylalanine methylester |
| 3T8H | 2012-08-01 | 1.45 | Thermolysin In Complex With UBTLN29 |
| 5FXN | 2016-03-16 | 1.45 | Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the ESRF RoboDiff goniometer |
| 5DPF | 2015-10-28 | 1.47 | Thermolysin in complex with inhibitor. |
| 3QH5 | 2012-01-04 | 1.5 | Structure of Thermolysin in complex with N-Carbobenzyloxy-L-aspartic acid and L-Phenylalanine Methyl Ester |
| 3T8G | 2012-08-01 | 1.5 | Thermolysin In Complex With UBTLN26 |
| 5FSS | 2016-10-26 | 1.5 | Structure of thermolysin prepared by the 'soak-and-freeze' method under 40 bar of krypton pressure |
| 6QF3 | 2019-07-10 | 1.52 | X-Ray structure of Thermolysin soaked with sodium aspartate on a silicon chip |
| 3MS3 | 2011-05-11 | 1.54 | Crystal structure of Thermolysin in complex with Aniline |
| 3QH1 | 2012-01-04 | 1.55 | Structure of Thermolysin in complex with N-benzyloxycarbonyl-L-aspartic acid |
| 3FXS | 2010-02-09 | 1.55 | Metal exchange in thermolysin |
| 4H57 | 2012-10-03 | 1.56 | Thermolysin inhibition |
| 3FV4 | 2010-02-09 | 1.56 | Thermolysin inhibition |
| 3T73 | 2012-08-01 | 1.6 | Thermolysin In Complex With UBTLN22 |
| 5UUB | 2017-03-08 | 1.6 | Tetragonal thermolysin cryocooled to 100 K with 25% xylose/25% mpd as cryoprotectant |
| 5UN3 | 2017-02-22 | 1.6 | Tetragonal thermolysin (295 K) in the presence of 50% xylose |
| 6TMN | 1989-01-09 | 1.6 | Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond |
| 5UUC | 2017-03-08 | 1.6 | Tetragonal thermolysin cryocooled to 100 K with 50% mpd as cryoprotectant |
| 1KJO | 2002-12-05 | 1.6 | Thermolysin complexed with Z-L-Threonine (benzyloxycarbonyl-L-Threonine) |
| 1KKK | 2002-12-10 | 1.6 | Thermolysin complexed with Z-L-Aspartic Acid (benzyloxycarbonyl-L-Aspartic Acid) |
| 1KEI | 2002-11-16 | 1.6 | Thermolysin (substrate-free) |
| 5UUE | 2017-03-08 | 1.6 | Tetragonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant |
| 3FB0 | 2009-12-08 | 1.6 | Metal exchange in thermolysin |
| 5UUA | 2017-03-08 | 1.6 | Tetragonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant |
| 5UU9 | 2017-03-08 | 1.6 | Tetragonal thermolysin cryocooled to 100 K with 40% xylose as cryoprotectant |
| 5UUD | 2017-03-08 | 1.6 | Tetragonal thermolysin cryocooled to 100 K with 50% dmf as cryoprotectant |
| 2TMN | 1989-01-09 | 1.6 | CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN |
| 5UU7 | 2017-03-08 | 1.6 | Tetragonal thermolysin (295 K) in the presence of 50% mpd |
| 8TLN | 1994-04-30 | 1.6 | STRUCTURAL COMPARISON SUGGESTS THAT THERMOLYSIN AND RELATED NEUTRAL PROTEASES UNDERGO HINGE-BENDING MOTION DURING CATALYSIS |
| 5TMN | 1989-01-09 | 1.6 | Slow-and fast-binding inhibitors of thermolysin display different modes of binding. crystallographic analysis of extended phosphonamidate transition-state analogues |
| 1KJP | 2002-12-05 | 1.6 | Thermolysin complexed with Z-L-Glutamic acid (benzyloxycarbonyl-L-Glutamic acid) |
| 2TLX | 2000-03-13 | 1.65 | THERMOLYSIN (NATIVE) |
| 3MSA | 2011-05-11 | 1.66 | Crystal structure of Thermolysin in complex with 3-Bromophenol |
| 3T8C | 2012-08-01 | 1.66 | Thermolysin In Complex With UBTLN30 |
| 3F28 | 2009-11-17 | 1.68 | Thermolysin inhibition |
| 1LND | 1995-05-08 | 1.7 | A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN |
| 1LNF | 1995-05-08 | 1.7 | A structural analysis of metal substitutions in thermolysin |
| 1Z9G | 2005-04-19 | 1.7 | Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (R)-retro-thiorphan |
| 1THL | 1994-01-31 | 1.7 | Thermolysin complexed with a novel glutaramide derivative, n-(1-(2(r,s)-carboxy-4-phenylbutyl) cyclopentylcarbonyl)-(s)-tryptophan |
| 4TMN | 1989-01-09 | 1.7 | SLOW-AND FAST-BINDING INHIBITORS OF THERMOLYSIN DISPLAY DIFFERENT MODES OF BINDING. CRYSTALLOGRAPHIC ANALYSIS OF EXTENDED PHOSPHONAMIDATE TRANSITION-STATE ANALOGUES |
| 1ZDP | 2005-04-26 | 1.7 | Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (S)-thiorphan |
| 1KS7 | 2003-01-11 | 1.7 | Thermolysin complexed with Z-D-Aspartic acid (benzyloxycarbonyl-D-Aspartic acid) |
| 3TMN | 1989-01-09 | 1.7 | THE BINDING OF L-VALYL-L-TRYPTOPHAN TO CRYSTALLINE THERMOLYSIN ILLUSTRATES THE MODE OF INTERACTION OF A PRODUCT OF PEPTIDE HYDROLYSIS |
| 5FSP | 2016-10-26 | 1.7 | Structure of thermolysin prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure |
| 1PE5 | 2004-06-08 | 1.7 | Thermolysin with tricyclic inhibitor |
| 1FJQ | 2001-04-18 | 1.7 | THERMOLYSIN (70% ACETONE SOAKED CRYSTALS) |
| 1LNE | 1995-05-08 | 1.7 | A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN |
| 1HYT | 1994-07-31 | 1.7 | RE-DETERMINATION AND REFINEMENT OF THE COMPLEX OF BENZYLSUCCINIC ACID WITH THERMOLYSIN AND ITS RELATION TO THE COMPLEX WITH CARBOXYPEPTIDASE A |
| 1KRO | 2003-01-10 | 1.7 | Thermolysin complexed with Z-D-Threonine (benzyloxycarbonyl-D-Threonine) |
| 6FHP | 2018-09-12 | 1.7 | DAIP in complex with a C-terminal fragment of thermolysin |
| 6QF2 | 2019-07-10 | 1.73 | X-Ray structure of Thermolysin crystallized on a silicon chip |
| 3FCQ | 2009-12-08 | 1.75 | Thermolysin inhibition |
| 2WHZ | 2010-05-12 | 1.75 | Dipeptide Inhibitors of Thermolysin |
| 1LNB | 1995-05-08 | 1.8 | A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN |
| 1KR6 | 2003-01-09 | 1.8 | Thermolysin complexed with Z-D-Glutamic acid (benzyloxycarbonyl-D-Glutamic acid) |
| 1LNC | 1995-05-08 | 1.8 | A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN |
| 6IG7 | 2018-11-07 | 1.8 | Crystal structure of thermolysin delivered in polyacrylamide using x-ray free electron laser |
| 1KL6 | 2002-12-11 | 1.8 | Thermolysin complexed with Z-L-Alanine (benzyloxycarbonyl-L-Alanine) |
| 4TNL | 2014-07-09 | 1.8 | 1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL |
| 1PE8 | 2004-06-08 | 1.8 | Thermolysin with monocyclic inhibitor |
| 3SSB | 2011-09-07 | 1.8 | Structure of Insect Metalloproteinase Inhibitor in Complex with Thermolysin |
| 1PE7 | 2004-06-08 | 1.82 | Thermolysin with bicyclic inhibitor |
| 6QAR | 2020-01-15 | 1.85 | Thermolysine under 2 kbar of argon |
| 2A7G | 2005-07-19 | 1.85 | On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength |
| 3N21 | 2011-05-25 | 1.87 | Crystal structure of Thermolysin in complex with S-1,2-Propandiol |
| 3EIM | 2009-10-06 | 1.88 | Metal exchange in Thermolysin |
| 1LNA | 1995-05-08 | 1.9 | A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN |
| 1KTO | 2003-01-17 | 1.9 | Thermolysin complexed with Z-D-Alanine (benzyloxycarbonyl-D-Alanine) |
| 5O8N | 2017-08-09 | 1.9 | Structure of thermolysin at room temperature via a method of acoustically induced rotation. |
| 4D91 | 2013-01-16 | 1.9 | Thermolysin In Complex With DMSO And Acetate |
| 5WR5 | 2017-08-16 | 1.9 | Thermolysin, liganded form with cryo condition 1 |
| 1TMN | 1989-01-09 | 1.9 | Binding of n-carboxymethyl dipeptide inhibitors to thermolysin determined by x-ray crystallography. a novel class of transition-state analogues for zinc peptidases |
| 1FJW | 2001-04-18 | 1.9 | THERMOLYSIN (50 MM PHENOL SOAKED) |
| 3FBO | 2009-12-08 | 1.92 | Metal exchange in Thermolysin |
| 3FOR | 2010-01-12 | 1.93 | Thermolysin Complexed with BNPA (2-Benzyl-3-Nitro Propanoic Acid Amide) |
| 2WI0 | 2010-05-12 | 1.95 | Dipeptide Inhibitors of Thermolysin |
| 3F2P | 2009-11-17 | 1.95 | Thermolysin inhibition |
| 7TLI | 2000-03-13 | 1.95 | THERMOLYSIN (90% ISOPROPANOL SOAKED CRYSTALS) |
| 3TLI | 2000-02-18 | 1.95 | THERMOLYSIN (10% ISOPROPANOL SOAKED CRYSTALS) |
| 3T2H | 2011-12-28 | 1.95 | Tetragonal thermolysin in the presence of TMAO |
| 4TLI | 2000-03-13 | 1.95 | THERMOLYSIN (25% ISOPROPANOL SOAKED CRYSTALS) |
| 2TLI | 2000-02-18 | 1.95 | THERMOLYSIN (5% ISOPROPANOL SOAKED CRYSTALS) |
| 3MSN | 2011-05-11 | 1.97 | Crystal structure of Thermolysin in complex with N-methylurea |
| 3FLF | 2010-01-12 | 1.97 | Thermolysin inhibition |
| 3LS7 | 2011-02-23 | 1.98 | Crystal structure of Thermolysin in complex with Xenon |
| 2G4Z | 2007-02-20 | 1.98 | anomalous substructure of thermolysin |
| 1FJU | 2001-04-18 | 2.0 | THERMOLYSIN (80% ACETONITRILE SOAKED CRYSTALS) |
| 6D5R | 2018-09-19 | 2.0 | Hexagonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant |
| 1FJ3 | 2001-04-18 | 2.0 | THERMOLYSIN (50% ACETONE SOAKED) |
| 1FJO | 2001-04-18 | 2.0 | THERMOLYSIN (60% ACETONE SOAKED CRYSTALS) |
| 1FJV | 2001-04-18 | 2.0 | THERMOLYSIN (60% ACETONITRILE SOAKED CRYSTALS) |
| 6D5U | 2018-09-19 | 2.0 | Hexagonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant |
| 6D5Q | 2018-09-19 | 2.0 | Hexagonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant |
| 5WR2 | 2017-08-16 | 2.0 | Thermolysin, SFX liganded form with oil-based carrier |
| 3T2J | 2011-12-28 | 2.0 | Tetragonal thermolysin in the presence of betaine |
| 6D5O | 2018-09-19 | 2.0 | Hexagonal thermolysin (295 K) in the presence of 50% DMF |
| 6D5T | 2018-09-19 | 2.0 | Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant |
| 5TAK | 2017-08-30 | 2.0 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 6D5S | 2018-09-19 | 2.0 | Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant |
| 1QF1 | 1999-12-29 | 2.0 | THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYLHEPTANOYL)-PHE-ALA. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE |
| 3ZI6 | 2013-05-01 | 2.0 | Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the GRob robot goniometer |
| 6D5N | 2018-10-31 | 2.0 | Hexagonal thermolysin (295) in the presence of 50% xylose |
| 5TAJ | 2017-08-30 | 2.03 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 5TAC | 2017-08-30 | 2.04 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 3FXP | 2010-02-09 | 2.05 | Thermolysin inhibition |
| 1TLI | 2000-02-18 | 2.05 | THERMOLYSIN (2% ISOPROPANOL SOAKED CRYSTALS) |
| 3NN7 | 2011-05-25 | 2.05 | Crystal structure of Thermolysin in complex with 2-bromoacetate |
| 1QF2 | 1999-12-29 | 2.06 | THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-GLY-(5-PHENYLPROLINE). PARAMETERS FOR ZN-MONODENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE |
| 5T9I | 2017-08-30 | 2.09 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 5TAD | 2017-08-30 | 2.09 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 3MSF | 2011-05-11 | 2.09 | Crystal structure of Thermolysin in complex with Urea |
| 1TLX | 2000-03-13 | 2.1 | THERMOLYSIN (NATIVE) |
| 3T2I | 2011-12-28 | 2.1 | Tetragonal thermolysin in the presence of sarcosine |
| 5T9Q | 2017-08-30 | 2.1 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 6TLI | 2000-03-13 | 2.1 | THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS) |
| 1OS0 | 2003-03-25 | 2.1 | Thermolysin with an alpha-amino phosphinic inhibitor |
| 5T9K | 2017-08-30 | 2.1 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 5TLI | 2000-03-13 | 2.1 | THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS) |
| 1Y3G | 2006-01-17 | 2.1 | Crystal Structure of a Silanediol Protease Inhibitor Bound to Thermolysin |
| 5WR3 | 2017-08-16 | 2.1 | Thermolysin, SFX liganded form with water-based carrier |
| 4OW3 | 2014-03-12 | 2.1 | Thermolysin structure determined by free-electron laser |
| 5WR4 | 2017-08-16 | 2.1 | Thermolysin, SFX unliganded form with oil-based carrier |
| 1GXW | 2002-12-05 | 2.18 | the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate |
| 3P7S | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 100K structure D (7.2 MGy) |
| 3P7R | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 100K structure C (4.9 MGy) |
| 1FJT | 2001-04-18 | 2.2 | THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS) |
| 6SEL | 2019-08-21 | 2.2 | Multicrystal structure of Thermolysin at room temperature using a multilayer monochromator. |
| 3P7V | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 160K structure C (4.8 MGy) |
| 3P7W | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 160K structure D (7.1 MGy) |
| 3P7P | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 100K structure A (0.1 MGy) |
| 3P7T | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 160K structure A (0.1 MGy) |
| 3P7U | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 160K structure B (2.4 MGy) |
| 1QF0 | 1999-12-29 | 2.2 | THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-PHE-TYR. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE |
| 3P7Q | 2010-12-08 | 2.2 | Radiation damage study of thermolysin - 100K structure B (2.5 MGy) |
| 8TLI | 2000-03-13 | 2.2 | THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS) |
| 5DLH | 2015-11-18 | 2.25 | SFX structure of thermolysin |
| 1L3F | 2002-07-03 | 2.3 | Thermolysin in the Absence of Substrate has an Open Conformation |
| 7TLN | 1983-03-09 | 2.3 | STRUCTURAL ANALYSIS OF THE INHIBITION OF THERMOLYSIN BY AN ACTIVE-SITE-DIRECTED IRREVERSIBLE INHIBITOR |
| 5TAI | 2017-08-30 | 2.3 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 5TLN | 1982-05-26 | 2.3 | BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS |
| 4TLN | 1982-05-26 | 2.3 | BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS |
| 1TLP | 1989-01-09 | 2.3 | CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN |
| 5TAE | 2017-08-30 | 2.3 | Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin |
| 5WR6 | 2017-08-16 | 2.3 | Thermolysin, liganded form with cryo condition 2 |
| 5UU8 | 2017-03-08 | 2.5 | Tetragonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant |
| 5K7T | 2017-04-05 | 2.5 | MicroED structure of thermolysin at 2.5 A resolution |
| 6D5P | 2018-09-19 | 3.0 | Hexagonal thermolysin cryocooled to 100 K with 20% xylose as cryoprotectant |