The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin.


Abstract

Differential scanning calorimetry has been used to study the thermal unfolding of the 255-316 C-terminal fragment of thermolysin. The concentration effect on the calorimetric transitions of the fragment in 0.1 M NaCl and 20 mM phosphate buffer, pH 7.5, shows that it behaves as a highly stable dimer in solution, within the concentration range 0.19-4.55 mg/ml, undergoing a reversible two-state thermal unfolding process. The thermodynamic parameters of unfolding (delta G = 60 +/- 6 kJ/(mol of dimer) at 20 degrees C) are similar to those normally observed for small, compact, globular proteins. This and previous studies [1989, Eur. J. Biochem. 180, 513-518] show that the 255-316 fragment folds into a stable, native-like globular structure. Study holds ProTherm entries: 10548, 10549, 10550, 10551, 10552, 10553, 10554, 10555 Extra Details: 255-316 C-terminal fragment differential scanning calorimetry; thermolysin; protein stability;,protein domain; domain folding

Submission Details

ID: WNzjPM7h3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:40 p.m.

Version: 1

Publication Details
Conejero-Lara F;De Filippis V;Fontana A;Mateo PL,FEBS Lett. (1994) The thermodynamics of the unfolding of an isolated protein subdomain. The 255-316 C-terminal fragment of thermolysin. PMID:8187875
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
5WR2 2016-11-29T00:00:00+0000 2.0 Thermolysin, SFX liganded form with oil-based carrier
5WR5 2016-11-29T00:00:00+0000 1.9 Thermolysin, liganded form with cryo condition 1
5WR6 2016-11-29T00:00:00+0000 2.3 Thermolysin, liganded form with cryo condition 2
5WR3 2016-11-29T00:00:00+0000 2.1 Thermolysin, SFX liganded form with water-based carrier
5WR4 2016-11-29T00:00:00+0000 2.1 Thermolysin, SFX unliganded form with oil-based carrier
6GHX 2018-05-09T00:00:00+0000 1.16 Alzheimer's Amyloid-Beta Peptide Fragment 31-35 in Complex with Cd-substituted Thermolysin
5DLH 2015-09-04T00:00:00+0000 2.25 SFX structure of thermolysin
6FHP 2018-01-15T00:00:00+0000 1.7 DAIP in complex with a C-terminal fragment of thermolysin
6FSM 2018-02-19T00:00:00+0000 1.39 Crystal structure of TCE-treated Thermolysin
5ONP 2017-08-04T00:00:00+0000 1.34 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Cd-substituted Thermolysin

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.4 Thermolysin P43133 THER_GEOSE
100.0 Thermolysin P00800 THER_BACTH