Seven of the 13 non-glycine contact amino acids in the hen (chicken) egg white lysozyme (HEWL) epitope for antibody Fab-10 each contribute < or =0.3 kcal/mol to the change in free energy (DeltaDeltaG(D)) from wild type (WT) when replaced by alanine (nullspots), and three others each give (0.7 < DeltaDeltaG(D) < or = 1. 0) kcal/mol (warm spots) (Rajpal et al. Protein Sci 1998;7:1868-1874). The low DeltaDeltaG(D) values introduced by alanine mutations present an opportunity to explore accurately their cumulative effects, as the sum of the combined DeltaDeltaG(D) values is not so large as to destabilize the complex beyond the range of accurate measurement. Substitution of six of the seven null spot residues by alanine leads to a cumulative DeltaDeltaG(D) = 2.25 +/- 0.04 kcal/mol, whereas the sum of the six individual changes is only -0.36 +/- 0.32 kcal/mol. The triple warm spot mutation generates a DeltaDeltaG(D) = 5.11 +/- 0.06 kcal/mol versus DeltaDeltaG(D) = 2.52 +/- 0.22 kcal/mol for the sum of the three individuals. The non-additivity in the individual DeltaDeltaG(D) values for the alanine mutations may indicate that these residues provide a conformationally stabilizing effect on the hot spot residues, each of which exhibits DeltaDeltaG(D) > 4.0 kcal/mol on alanine substitution. Study holds ProTherm entries: 8037, 8038, 8039, 8040, 8041, 8042, 10286 Extra Details: hen egg white lysozyme; monoclonal antibody HyHEL-10;,association dissociation kinetics; multiple alanine mutations
ID: UwLDVwez
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:35 p.m.
Version: 1
Number of data points | 7 |
Proteins | Lysozyme C ; Lysozyme C |
Unique complexes | 6 |
Assays/Quantities/Protocols | Experimental Assay: Tm buffers:phosphate: 66 mM ; Experimental Assay: Tm buffers:Potassium phosphate: 66 mM |
Libraries | Mutations for sequence KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL |
Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Percent Identity | Matching Chains | Protein | Accession | Entry Name |
---|---|---|---|---|
100.0 | Lysozyme C | P00698 | LYSC_CHICK | |
96.9 | Lysozyme C | P00700 | LYSC_COLVI | |
96.9 | Lysozyme C | P00699 | LYSC_CALCC | |
96.9 | Lysozyme C | Q7LZQ0 | LYSC_CATWA | |
96.9 | Lysozyme C | Q7LZP9 | LYSC_LOPIM | |
96.1 | Lysozyme C | Q7LZI3 | LYSC_TRASA | |
95.3 | Lysozyme C | P00701 | LYSC_COTJA | |
96.1 | Lysozyme C | P19849 | LYSC_PAVCR | |
95.3 | Lysozyme C | P22910 | LYSC_CHRAM | |
95.3 | Lysozyme C | Q7LZT2 | LYSC_TRATE | |
95.2 | Lysozyme C | P00703 | LYSC_MELGA | |
92.2 | Lysozyme C | P00704 | LYSC_NUMME | |
93.0 | Lysozyme C | P24364 | LYSC_LOPLE | |
94.6 | Lysozyme C | P24533 | LYSC_SYRRE | |
93.2 | Lysozyme C | P00702 | LYSC_PHACO | |
93.0 | Lysozyme C | P81711 | LYSC_SYRSO | |
92.3 | Lysozyme C | P49663 | LYSC_PHAVE |