Diffusional barrier crossing in a two-state protein folding reaction.


There has been some debate as to whether protein folding involves diffusive chain motions and thus depends on solvent viscosity. The interpretation of folding kinetics in viscous solvents has remained difficult and controversial, in that viscogenic agents affect folding rates not only by increasing solvent viscosity but also by increasing protein stability. By carefully choosing experimental conditions, we can now eliminate the effect on stability and show that the folding dynamics of the cold shock protein CspB are viscosity dependent. Thus Kramers' theory of reaction rates rather than transition state theory should be used to describe this folding reaction. Study holds ProTherm entries: 15082, 15083, 15084, 15085, 15086, 15087, 15088, 15089, 15090 Extra Details: diffusive chain motions; CspB; viscosity dependent

Submission Details

ID: Umz5HNLu3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:45 p.m.

Version: 1

Publication Details
Jacob M;Geeves M;Holtermann G;Schmid FX,Nat. Struct. Biol. (1999) Diffusional barrier crossing in a two-state protein folding reaction. PMID:10504725
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Cold shock protein CspB P32081 CSPB_BACSU
92.3 Cold shock protein CspB Q81K90 CSPD_BACAN
92.3 Cold shock protein CspB Q816H3 CSPD_BACCR
96.5 Cold shock protein CspB P41018 CSPB_SPOGL