Diffusional barrier crossing in a two-state protein folding reaction.


Abstract

There has been some debate as to whether protein folding involves diffusive chain motions and thus depends on solvent viscosity. The interpretation of folding kinetics in viscous solvents has remained difficult and controversial, in that viscogenic agents affect folding rates not only by increasing solvent viscosity but also by increasing protein stability. By carefully choosing experimental conditions, we can now eliminate the effect on stability and show that the folding dynamics of the cold shock protein CspB are viscosity dependent. Thus Kramers' theory of reaction rates rather than transition state theory should be used to describe this folding reaction. Study holds ProTherm entries: 15082, 15083, 15084, 15085, 15086, 15087, 15088, 15089, 15090 Extra Details: diffusive chain motions; CspB; viscosity dependent

Submission Details

ID: Umz5HNLu3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:45 p.m.

Version: 1

Publication Details
Jacob M;Geeves M;Holtermann G;Schmid FX,Nat. Struct. Biol. (1999) Diffusional barrier crossing in a two-state protein folding reaction. PMID:10504725
Additional Information

Sequence Assay Result Units