Contribution of hydrogen bonds to the conformational stability of human lysozyme: calorimetry and X-ray analysis of six tyrosine --> phenylalanine mutants.


Abstract

The contribution of hydrogen bonds to the conformational stability of human lysozyme was investigated by the combination of calorimetric and X-ray analyses of six Tyr --> Phe mutants. Unfolding Delta G and unfolding Delta H values of the Tyr --> Phe mutant proteins were changed by from +0.3 to -4.0 kJ/mol and from 0 to -16 kJ/mol, respectively, compared to those of the wild-type protein. The net contribution of a hydrogen bond at a specific site to stability (Delta Gwild/HB), considering factors affected by substitutions, was evaluated on the basis of X-ray structures of the mutant proteins. In the present study, one of six mutant proteins was suitable for evaluating the strength of the hydrogen bond. Delta Gwild/HB for the intramolecular hydrogen bond at Tyr124 was evaluated to be 7.5 kJ/mol. Results of the analysis of other mutants also suggest that hydrogen bonds of the hydroxyl group of Tyr, including the hydrogen bond with a water molecule, contribute to the stabilization of the human lysozyme. Study holds ProTherm entries: 3399, 3400, 3401, 3402, 3403, 3404, 3405, 4189, 4190, 4191, 4192, 4193, 4194, 4195, 4196, 4197, 4198, 4199, 4200, 4201, 4202, 4203, 4204, 4205, 4206, 4207, 4208, 4209, 4210, 4211, 4212, 4213, 4214, 14203, 14204, 14205, 14206, 14207, 14208 Extra Details: conformational stability; hydrogen bonds; tyrosine;,phenylalanine; human lysozyme

Submission Details

ID: Umgda4Vs

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:21 p.m.

Version: 1

Publication Details
Yamagata Y;Kubota M;Sumikawa Y;Funahashi J;Takano K;Fujii S;Yutani K,Biochemistry (1998) Contribution of hydrogen bonds to the conformational stability of human lysozyme: calorimetry and X-ray analysis of six tyrosine --> phenylalanine mutants. PMID:9649316
Additional Information

Study Summary

Number of data points 151
Proteins Lysozyme C ; Lysozyme C ; Lysozyme C ; Lysozyme C ; Lysozyme C ; Lysozyme C ; Lysozyme C ; Lysozyme C
Unique complexes 7
Assays/Quantities/Protocols Experimental Assay: dCp ionic:-: -, temp:64.9 C ; Experimental Assay: ddG ; Experimental Assay: dCp pH:2.53, ionic:: ; Experimental Assay: dHcal pH:2.53 ; Experimental Assay: Tm pH:2.53 ; Experimental Assay: dHvH pH:2.53 ; Experimental Assay: dCp pH:2.68, ionic:: ; Experimental Assay: dHcal pH:2.68 ; Experimental Assay: Tm pH:2.68 ; Experimental Assay: dHvH pH:2.68 ; Experimental Assay: dCp ionic:: , pH:2.73 ; Experimental Assay: dHcal pH:2.73 ; Experimental Assay: Tm pH:2.73 ; Experimental Assay: dHvH pH:2.73 ; Experimental Assay: dCp pH:3.0, ionic:: ; Experimental Assay: dHcal pH:3.0 ; Experimental Assay: Tm pH:3.0 ; Experimental Assay: dHvH pH:3.0 ; Experimental Assay: dCp pH:3.17, ionic:: ; Experimental Assay: dHcal pH:3.17 ; Experimental Assay: Tm pH:3.17 ; Experimental Assay: dHvH pH:3.17 ; Experimental Assay: dCp pH:2.69, ionic:: ; Experimental Assay: dHcal pH:2.69 ; Experimental Assay: Tm pH:2.69 ; Experimental Assay: dHvH pH:2.69 ; Experimental Assay: dCp pH:2.82, ionic:: ; Experimental Assay: dHcal pH:2.82 ; Experimental Assay: Tm pH:2.82 ; Experimental Assay: dHvH pH:2.82 ; Experimental Assay: dCp pH:2.99, ionic:: ; Experimental Assay: dHcal pH:2.99 ; Experimental Assay: Tm pH:2.99 ; Experimental Assay: dHvH pH:2.99 ; Experimental Assay: dCp pH:2.48, ionic:: ; Experimental Assay: dHcal pH:2.48 ; Experimental Assay: Tm pH:2.48 ; Experimental Assay: dHvH pH:2.48 ; Experimental Assay: dCp ionic:: , pH:2.67 ; Experimental Assay: dHcal pH:2.67 ; Experimental Assay: Tm pH:2.67 ; Experimental Assay: dHvH pH:2.67 ; Experimental Assay: dCp pH:3.13, ionic:: ; Experimental Assay: dHcal pH:3.13 ; Experimental Assay: Tm pH:3.13 ; Experimental Assay: dHvH pH:3.13 ; Experimental Assay: dCp pH:2.71, ionic:: ; Experimental Assay: dHcal pH:2.71 ; Experimental Assay: Tm pH:2.71 ; Experimental Assay: dHvH pH:2.71 ; Experimental Assay: dCp pH:2.92, ionic:: ; Experimental Assay: dHcal pH:2.92 ; Experimental Assay: Tm pH:2.92 ; Experimental Assay: dHvH pH:2.92 ; Experimental Assay: dCp pH:3.1, ionic:: ; Experimental Assay: dHcal pH:3.1 ; Experimental Assay: Tm pH:3.1 ; Experimental Assay: dHvH pH:3.1 ; Experimental Assay: dCp ionic:: , pH:3.25 ; Experimental Assay: dHcal pH:3.25 ; Experimental Assay: Tm pH:3.25 ; Experimental Assay: dHvH pH:3.25 ; Experimental Assay: dCp pH:2.58, ionic:: ; Experimental Assay: dHcal pH:2.58 ; Experimental Assay: Tm pH:2.58 ; Experimental Assay: dHvH pH:2.58 ; Experimental Assay: dCp pH:2.74, ionic:: ; Experimental Assay: dHcal pH:2.74 ; Experimental Assay: Tm pH:2.74 ; Experimental Assay: dHvH pH:2.74 ; Experimental Assay: dCp pH:2.89, ionic:: ; Experimental Assay: dHcal pH:2.89 ; Experimental Assay: Tm pH:2.89 ; Experimental Assay: dHvH pH:2.89 ; Experimental Assay: dCp pH:3.06, ionic:: ; Experimental Assay: dHcal pH:3.06 ; Experimental Assay: Tm pH:3.06 ; Experimental Assay: dHvH pH:3.06 ; Experimental Assay: dCp pH:3.18, ionic:: ; Experimental Assay: dHcal pH:3.18 ; Experimental Assay: Tm pH:3.18 ; Experimental Assay: dHvH pH:3.18 ; Experimental Assay: dTm ; Experimental Assay: dHcal pH:2.7 ; Experimental Assay: Tm pH:2.7 ; Experimental Assay: dHvH pH:2.7 ; Experimental Assay: dCp ionic::
Libraries Mutations for sequence KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1IY4 2002-07-31 Solution structure of the human lysozyme at 35 degree C
1IY3 2002-07-31 Solution Structure of the Human lysozyme at 4 degree C
2NWD 2006-12-19 1.04 Structure of chemically synthesized human lysozyme at 1 Angstrom resolution
5LSH 2017-06-21 1.06 human lysozyme in complex with a tetrasaccharide fragment of the O-chain of LPS from Klebsiella pneumoniae
1JSF 1998-04-29 1.15 FULL-MATRIX LEAST-SQUARES REFINEMENT OF HUMAN LYSOZYME
1IWT 2002-09-04 1.4 Crystal Structure Analysis of Human lysozyme at 113K.
1IWW 2002-09-04 1.4 Crystal Structure Analysis of Human lysozyme at 152K.
1JWR 2001-09-19 1.4 Crystal structure of human lysozyme at 100 K
1IWV 2002-09-04 1.4 Crystal Structure Analysis of Human lysozyme at 147K.
1IWU 2002-09-04 1.4 Crystal Structure Analysis of Human lysozyme at 127K.
1IWX 2002-09-04 1.4 Crystal Structure Analysis of Human lysozyme at 161K.
1IWY 2002-09-04 1.4 Crystal Structure Analysis of Human lysozyme at 170K.
1IWZ 2002-09-04 1.48 Crystal Structure Analysis of Human lysozyme at 178K.
3FE0 2009-12-08 1.5 X-ray crystal structure of wild type human lysozyme in D2O
1LZR 1995-04-20 1.5 STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0
1REX 1997-02-12 1.5 NATIVE HUMAN LYSOZYME
1LZ1 1985-01-02 1.5 REFINEMENT OF HUMAN LYSOZYME AT 1.5 ANGSTROMS RESOLUTION. ANALYSIS OF NON-BONDED AND HYDROGEN-BOND INTERACTIONS
4R0P 2014-12-17 1.52 Ifqins, an amyloid forming segment from human lysozyme spanning residues 56-61
1LMT 1995-03-31 1.6 STRUCTURE OF A CONFORMATIONALLY CONSTRAINED ARG-GLY-ASP SEQUENCE INSERTED INTO HUMAN LYSOZYME
1LZS 1995-04-20 1.6 STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0
1JKC 1997-05-15 1.6 HUMAN LYSOZYME MUTANT WITH TRP 109 REPLACED BY PHE
1JKA 1997-05-15 1.66 HUMAN LYSOZYME MUTANT WITH GLU 35 REPLACED BY ASP
1JKB 1997-05-15 1.66 HUMAN LYSOZYME MUTANT WITH GLU 35 REPLACED BY ALA
1REZ 1997-02-12 1.7 HUMAN LYSOZYME-N-ACETYLLACTOSAMINE COMPLEX
1TAY 1993-01-15 1.7 DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME
1REY 1997-02-12 1.7 HUMAN LYSOZYME-N,N'-DIACETYLCHITOBIOSE COMPLEX
1GE1 2000-11-08 1.7 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
1TCY 1993-01-15 1.7 DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME
1TDY 1993-01-15 1.7 DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME
1TBY 1993-01-15 1.77 DISSECTION OF THE FUNCTIONAL ROLE OF STRUCTURAL ELEMENTS OF TYROSINE-63 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME
133L 1993-10-31 1.77 ROLE OF ARG 115 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME. X-RAY STRUCTURE OF HIS 115 AND GLU 115 MUTANTS
134L 1993-10-31 1.77 ROLE OF ARG 115 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME. X-RAY STRUCTURE OF HIS 115 AND GLU 115 MUTANTS
1LAA 1993-01-15 1.77 X-RAY STRUCTURE OF GLU 53 HUMAN LYSOZYME
1GE0 2000-11-08 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
1HNL 1995-02-14 1.8 CRYSTAL STRUCTURE OF A GLUTATHIONYLATED HUMAN LYSOZYME: A FOLDING INTERMEDIATE MIMIC IN THE FORMATION OF A DISULFIDE BOND
1B7O 1999-02-02 1.8 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
1EQE 2000-04-19 1.8 CRYSTAL STRUCTURES OF SALT BRIDGE MUTANTS OF HUMAN LYSOZYME
2HEF 1998-01-14 1.8 CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY
2BQC 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GF8 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2MEE 1998-07-15 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GB3 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1C43 1999-08-20 1.8 MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES
1GBX 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1LOZ 1997-04-01 1.8 AMYLOIDOGENIC VARIANT (I56T) VARIANT OF HUMAN LYSOZYME
1GF9 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2BQD 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1C45 1999-08-20 1.8 MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES
1GBY 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1WQO 1998-07-01 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1IP3 2001-11-14 1.8 G68A HUMAN LYSOZYME
2BQI 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1OUG 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V2A MUTANT
2LHM 1992-04-15 1.8 CRYSTAL STRUCTURES OF THE APO-AND HOLOMUTANT HUMAN LYSOZYMES WITH AN INTRODUCED CA2+ BINDING SITE
1LZ5 1993-10-31 1.8 STRUCTURAL AND FUNCTIONAL ANALYSES OF THE ARG-GLY-ASP SEQUENCE INTRODUCED INTO HUMAN LYSOZYME
1WQP 1998-07-01 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
2HEC 1998-01-14 1.8 CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY
1IP5 2001-11-14 1.8 G105A HUMAN LYSOZYME
1EQ4 2000-04-19 1.8 CRYSTAL STRUCTURES OF SALT BRIDGE MUTANTS OF HUMAN LYSOZYME
1IP6 2001-11-14 1.8 G127A HUMAN LYSOZYME
1GF5 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
1OUD 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V121A MUTANT
1OUF 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V130A MUTANT
1GB2 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1GFV 2000-12-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2BQE 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
2BQB 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
2BQA 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1B7L 1999-01-27 1.8 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
1GFH 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2MEH 1998-07-15 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GFK 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1OUC 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V110A MUTANT
2BQL 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GFA 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1WQM 1998-07-01 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1IX0 2003-07-22 1.8 I59A-3SS human lysozyme
1YAP 1996-04-03 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS
1GBZ 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1OUE 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V125A MUTANT
1GF3 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
2BQK 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GF0 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
2ZIL 2009-01-06 1.8 Crystal Structure of Human Lysozyme from Urine
2ZWB 2009-12-08 1.8 Neutron crystal structure of wild type human lysozyme in D2O
1CJ9 1999-04-30 1.8 T40V MUTANT HUMAN LYSOZYME
1IP2 2001-11-14 1.8 G48A HUMAN LYSOZYME
1GFU 2000-12-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1GF6 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
1OUH 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V74A MUTANT
1OUI 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V93A MUTANT
1EQ5 2000-04-19 1.8 CRYSTAL STRUCTURES OF SALT BRIDGE MUTANTS OF HUMAN LYSOZYME
1YAN 1996-04-03 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS
1LHJ 1994-01-31 1.8 ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS
2MEB 1998-07-15 1.8 CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS
1LYY 1997-04-01 1.8 AMYLOIDOGENIC VARIANT (ASP67HIS) OF HUMAN LYSOZYME
1LHL 1994-01-31 1.8 ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS
1CKD 1999-04-30 1.8 T43V MUTANT HUMAN LYSOZYME
1I1Z 2001-02-28 1.8 MUTANT HUMAN LYSOZYME (Q86D)
1CJ6 1999-04-30 1.8 T11A MUTANT HUMAN LYSOZYME
1LHH 1994-01-31 1.8 ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS
1LZ6 1993-10-31 1.8 STRUCTURAL AND FUNCTIONAL ANALYSES OF THE ARG-GLY-ASP SEQUENCE INTRODUCED INTO HUMAN LYSOZYME
1GFE 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1CJ8 1999-04-30 1.8 T40A MUTANT HUMAN LYSOZYME
2BQM 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GFT 2000-12-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1GFG 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2BQG 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1DI3 1999-12-08 1.8 ROLE OF AMINO ACID RESIDUES AT TURNS IN THE CONFORMATIONAL STABILITY AND FOLDING OF HUMAN LYSOZYME
1GFR 2000-12-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
4ML7 2014-07-23 1.8 Crystal structure of Brucella abortus PliC in complex with human lysozyme
1GAZ 2000-07-27 1.8 Crystal Structure of Mutant Human Lysozyme Substituted at the Surface Positions
1GDX 2000-11-08 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
1GFJ 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2BQJ 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
2MEF 1998-07-15 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GDW 2000-11-01 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
1B7R 1999-02-02 1.8 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
2BQF 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GEZ 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
1WQQ 1998-07-01 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GAY 2000-06-29 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1GF7 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
1YAM 1996-04-03 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS
1OUA 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE I56T MUTANT
2BQH 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GB9 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2MED 1998-07-15 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1LZ4 1993-10-31 1.8 ENTHALPIC DESTABILIZATION OF A MUTANT HUMAN LYSOZYME LACKING A DISULFIDE BRIDGE BETWEEN CYSTEINE-77 AND CYSTEINE-95
1CJ7 1999-04-30 1.8 T11V MUTANT HUMAN LYSOZYME
2BQO 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
2HEE 1998-01-14 1.8 CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY
1B7N 1999-01-27 1.8 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
2MEG 1998-07-15 1.8 CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS.
1I22 2001-02-28 1.8 MUTANT HUMAN LYSOZYME (A83K/Q86D/A92D)
1CKC 1999-04-30 1.8 T43A MUTANT HUMAN LYSOZYME
1GBW 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1GF4 2001-04-18 1.8 BURIED POLAR MUTANT HUMAN LYSOZYME
1INU 2000-12-20 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1WQN 1998-07-01 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1OUB 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V100A MUTANT
1GB0 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1YAO 1996-04-03 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS
1YAQ 1996-04-03 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: CALORIMETRIC STUDIES AND X-RAY STRUCTURAL ANALYSIS OF THE FIVE ISOLEUCINE TO VALINE MUTANTS
1GE4 2000-11-08 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
1IP1 2001-11-14 1.8 G37A HUMAN LYSOZYME
1LHK 1994-01-31 1.8 ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS
2BQN 1998-08-12 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1LHI 1994-01-31 1.8 ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS
1GBO 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
2MEI 1998-07-15 1.8 CONTRIBUTION OF HYDROPHOBIC EFFECT TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
1GB7 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1WQR 1998-07-01 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME
2HEA 1998-01-14 1.8 CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY
1GB5 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1LHM 1992-04-15 1.8 THE CRYSTAL STRUCTURE OF A MUTANT LYSOZYME C77(SLASH)95A WITH INCREASED SECRETION EFFICIENCY IN YEAST
1IP4 2001-11-14 1.8 G72A HUMAN LYSOZYME
3LHM 1992-04-15 1.8 CRYSTAL STRUCTURES OF THE APO-AND HOLOMUTANT HUMAN LYSOZYMES WITH AN INTRODUCED CA2+ BINDING SITE
1GB6 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1GB8 2000-07-27 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS
1OUJ 1997-02-12 1.8 CONTRIBUTION OF HYDROPHOBIC RESIDUES TO THE STABILITY OF HUMAN LYSOZYME: X-RAY STRUCTURE OF THE V99A MUTANT
207L 1996-10-14 1.8 MUTANT HUMAN LYSOZYME C77A
2HED 1998-01-14 1.8 CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY
1JKD 1997-05-15 1.8 HUMAN LYSOZYME MUTANT WITH TRP 109 REPLACED BY ALA
1B5U 1999-01-20 1.8 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANT
1CKF 1999-04-30 1.8 T52A MUTANT HUMAN LYSOZYME
1GE3 2000-11-08 1.8 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
2ZIK 2009-01-06 1.81 Crystal Structure of Human Lysozyme from Pichia pastoris
1QSW 2001-08-08 1.85 CRYSTAL STRUCTURE ANALYSIS OF A HUMAN LYSOZYME MUTANT W64C C65A
3EBA 2008-12-02 1.85 CAbHul6 FGLW mutant (humanized) in complex with human lysozyme
1OP9 2003-10-14 1.86 Complex of human lysozyme with camelid VHH HL6 antibody fragment
1IP7 2001-11-14 1.9 G129A HUMAN LYSOZYME
1I20 2001-02-28 1.9 MUTANT HUMAN LYSOZYME (A92D)
2ZIJ 2009-01-06 1.9 Crystal Structure of Human Lysozyme Expressed in E. coli.
4I0C 2013-10-09 1.95 The structure of the camelid antibody cAbHuL5 in complex with human lysozyme
1D6Q 2000-01-21 1.96 HUMAN LYSOZYME E102 MUTANT LABELLED WITH 2',3'-EPOXYPROPYL GLYCOSIDE OF N-ACETYLLACTOSAMINE
1DI4 1999-12-08 2.0 ROLE OF AMINO ACID RESIDUES AT TURNS IN THE CONFORMATIONAL STABILITY AND FOLDING OF HUMAN LYSOZYME
1B5X 1999-01-20 2.0 Contribution of hydrogen bonds to the conformational stability of human lysozyme: calorimetry and x-ray analysis of six ser->ala mutants
1GE2 2000-11-08 2.0 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT LEFT-HANDED HELICAL POSITIONS
1B7Q 1999-02-02 2.0 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
1UBZ 2003-04-22 2.0 Crystal structure of Glu102-mutant human lysozyme doubly labeled with 2',3'-epoxypropyl beta-glycoside of N-acetyllactosamine
1B7P 1999-01-25 2.0 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
1CKH 1999-04-30 2.0 T70V MUTANT HUMAN LYSOZYME
1B7S 1999-02-02 2.0 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
3LN2 2011-02-16 2.04 Crystal Structure of a Charge Engineered Human Lysozyme Variant
1GEV 2001-04-18 2.1 BURIED POLAR MUTANT HUMAN LYSOZYME
1REM 1998-07-15 2.1 HUMAN LYSOZYME WITH MAN-B1,4-GLCNAC COVALENTLY ATTACHED TO ASP53
1B5W 1999-01-20 2.17 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS
1B5V 1999-01-20 2.17 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS
1B7M 1999-01-27 2.2 VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
1B5Y 1999-01-20 2.2 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS
1C46 1999-08-20 2.2 MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES
2MEC 1998-07-15 2.2 CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS
1DI5 1999-12-08 2.2 ROLE OF AMINO ACID RESIDUES AT TURNS IN THE CONFORMATIONAL STABILITY AND FOLDING OF HUMAN LYSOZYME
2MEA 1998-07-15 2.2 CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS
1CKG 1999-05-05 2.2 T52V MUTANT HUMAN LYSOZYME
1B5Z 1999-02-02 2.2 CONTRIBUTION OF HYDROGEN BONDS TO THE CONFORMATIONAL STABILITY OF HUMAN LYSOZYME: CALORIMETRY AND X-RAY ANALYSIS OF SIX SER->ALA MUTANTS
2HEB 1998-01-28 2.2 CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY
208L 1996-10-14 2.2 MUTANT HUMAN LYSOZYME C77A
1D6P 2000-01-21 2.23 HUMAN LYSOZYME L63 MUTANT LABELLED WITH 2',3'-EPOXYPROPYL N,N'-DIACETYLCHITOBIOSE
1RE2 1999-05-05 2.3 HUMAN LYSOZYME LABELLED WITH TWO 2',3'-EPOXYPROPYL BETA-GLYCOSIDE OF N-ACETYLLACTOSAMINE
1C7P 2000-04-05 2.4 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME WITH FOUR EXTRA RESIDUES (EAEA) AT THE N-TERMINAL
1IOC 2002-10-09 2.4 CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME, EAEA-I56T
5LVK 2016-09-28 2.49 Human Lysozyme soaked with [H2Ind][trans-RuCl4(DMSO)(HInd)]
1W08 2004-06-10 2.5 STRUCTURE OF T70N HUMAN LYSOZYME

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
96.9 Lysozyme C P79180 LYSC_HYLLA
99.2 Lysozyme C P79239 LYSC_PONPY
100.0 Lysozyme C P61628 LYSC_PANTR
100.0 Lysozyme C P61627 LYSC_PANPA
100.0 Lysozyme C P61626 LYSC_HUMAN
100.0 Lysozyme C P79179 LYSC_GORGO