On the non-respect of the thermodynamic cycle by DsbA variants.


Abstract

The mechanism of the disulfide-bond forming enzyme DsbA depends on the very low pKa of a cysteine residue in its active-site and on the relative instability of the oxidized enzyme compared to the reduced one. A thermodynamic cycle has been used to correlate its redox properties to the difference in the free energies of folding (deltadeltaGred/ox) of the oxidized and reduced forms. However, the relation was proved unsatisfied for a number of DsbA variants. In this study, we investigate the thermodynamic and redox properties of a highly destabilized variant DsbA(P151A) (substitution of cis-Pro151 by an alanine) by the means of intrinsic tryptophan fluorescence and by high-sensitivity differential scanning calorimetry (HS-DSC). When the value of deltadeltaGred/ox obtained fluorimetrically for DsbA(P151A) does not correlate with the value expected from its redox potential, the value of deltadeltaGred/ox provided by HS-DSC are in perfect agreement with the predicted thermodynamic cycle for both wild-type and variant. HS-DSC data indicate that oxidized wild-type enzyme and the reduced forms of both wild-type and variant unfold according to a two-state mechanism. Oxidized DsbA(P151A) shows a deviation from two-state behavior that implies the loss of interdomain cooperativity in DsbA caused by Pro151 substitution. The presence of chaotrope in fluorimetric measurements could facilitate domain uncoupling so that the fluorescence probe (Trp76) does not reflect the whole unfolding process of DsbA(P151A) anymore. Thus, theoretical thermodynamic cycle is respected when an appropriate method is applied to DsbA unfolding under conditions in which protein domains still conserve their cooperativity. Study holds ProTherm entries: 6712, 6713, 6714, 6715, 6716, 6717, 6718, 6719, 6720, 6721, 6722, 6723 Extra Details: additive : EDTA(1 mM), calorimetry; DsbA; redox potential; stability;,themodynamic cycle

Submission Details

ID: U7bKHhs83

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:32 p.m.

Version: 1

Publication Details
Moutiez M;Burova TV;Haertlé T;Quéméneur E,Protein Sci. (1999) On the non-respect of the thermodynamic cycle by DsbA variants. PMID:10210189
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2LEG 2011-10-26 Membrane protein complex DsbB-DsbA structure by joint calculations with solid-state NMR and X-ray experimental data
1A24 1998-09-16 SOLUTION NMR STRUCTURE OF REDUCED DSBA FROM ESCHERICHIA COLI, FAMILY OF 20 STRUCTURES
2NDO 2017-02-08 Structure of EcDsbA-sulfonamide1 complex
1A23 1998-09-16 SOLUTION NMR STRUCTURE OF REDUCED DSBA FROM ESCHERICHIA COLI, MINIMIZED AVERAGE STRUCTURE
5QO9 2020-02-05 1.59 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_4)
5QNY 2020-02-05 1.6 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_24)
5QOF 2020-02-05 1.64 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_8)
5QO2 2020-02-05 1.64 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_3)
5QOE 2020-02-05 1.67 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_7)
5QMI 2020-02-05 1.67 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F12_2)
5QNP 2020-02-05 1.67 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_16)
5QOC 2020-02-05 1.67 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_50)
6POH 2019-11-06 1.67 Crystal Structure of EcDsbA in complex alkyl ether 21
5QNQ 2020-02-05 1.67 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_17)
5QNX 2020-02-05 1.68 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_23)
5QOB 2020-02-05 1.7 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_5)
1FVK 1997-08-20 1.7 THE 1.7 ANGSTROM STRUCTURE OF WILD TYPE DISULFIDE BOND FORMATION PROTEIN (DSBA)
5QO3 2020-02-05 1.73 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_31)
5QO8 2020-02-05 1.73 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_38)
6PVY 2019-12-25 1.74 E.coli DsbA in complex with benzofuran compound 26 ([6-(3-methoxyphenoxy)-1-benzofuran-3-yl]acetic acid)
5QNR 2020-02-05 1.75 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_18)
5QNL 2020-02-05 1.75 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_12)
5QNV 2020-02-05 1.75 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_21)
5QNT 2020-02-05 1.75 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_2)
5QO5 2020-02-05 1.77 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_34)
6POI 2019-11-06 1.77 Crystal Structure of EcDsbA in complex phenyl ether 25
5QNS 2020-02-05 1.78 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_19)
5QO4 2020-02-05 1.78 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_32)
4ZIJ 2016-05-11 1.78 Crystal structure of E.Coli DsbA in complex with 2-(4-iodophenylsulfonamido) benzoic acid
5QNN 2020-02-05 1.79 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_14)
5QO6 2020-02-05 1.79 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_36)
5QNZ 2020-02-05 1.79 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_25)
5QOG 2020-02-05 1.79 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_9)
6POQ 2019-11-06 1.8 Crystal Structure of EcDsbA in complex with anisidine 16
5QME 2020-02-05 1.83 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure EDC_1)
5QO0 2020-02-05 1.83 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_26)
5QO1 2020-02-05 1.84 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_28)
5QMK 2020-02-05 1.84 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F2_1)
5QNJ 2020-02-05 1.86 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_10)
5QLA 2020-02-05 1.86 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C1_1)
5QNM 2020-02-05 1.86 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_13)
5QNU 2020-02-05 1.86 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_20)
5QLQ 2020-02-05 1.87 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D1_2)
5QN8 2020-02-05 1.87 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H2_1)
5QNW 2020-02-05 1.88 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_22)
5QKP 2020-02-05 1.88 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A7_3)
5QMC 2020-02-05 1.89 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E8_1)
5QLH 2020-02-05 1.89 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C4_1)
5QN5 2020-02-05 1.89 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G8_2)
5QND 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H7_2)
5QMB 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E7_1)
5QKN 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A5_2)
5QLX 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D4_1)
5QKT 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A9_1)
5QN6 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G9_1)
5QNG 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure HATU_1)
3DKS 2009-05-12 1.9 DsbA substrate complex
5QMQ 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F8_1)
1ACV 1997-10-15 1.9 DSBA MUTANT H32S
6PBI 2020-05-06 1.9 Crystal Structure of EcDsbA in a complex with purified morpholine 8
5QKG 2020-02-05 1.9 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A2_3)
5QNK 2020-02-05 1.9 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_11)
6PGJ 2020-05-06 1.9 Crystal Structure of EcDsbA in a complex with unpurified reaction product A5 (Morpholine carboxylic acid 7)
5QNB 2020-02-05 1.91 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H5_1)
6PG2 2020-05-06 1.91 Crystal Structure of EcDsbA in a complex with unpurified reaction product H5 (morpholine 8)
5QOD 2020-02-05 1.91 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_6)
5QMV 2020-02-05 1.91 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G12_1)
5QM0 2020-02-05 1.92 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D7_1)
6PD7 2020-05-06 1.92 Crystal Structure of EcDsbA in a complex with purified morpholine carboxylic acid 7
5QM2 2020-02-05 1.92 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D9_1)
5QMX 2020-02-05 1.92 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G3_1)
6PLI 2020-05-06 1.93 Crystal Structure of EcDsbA in a complex with purified oxadiazole 11
5QN9 2020-02-05 1.94 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H3_1)
6PMF 2019-11-06 1.95 Crystal Structure of EcDsbA in complex with aniline 15
5QNO 2020-02-05 1.95 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_15)
5QLE 2020-02-05 1.95 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C2_1)
5QMM 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F4_1)
6PDH 2020-05-06 1.96 Crystal Structure of EcDsbA in a complex with purified pyrazole 9
5QLL 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C8_1)
5QL3 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B5_1)
5QLJ 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C6_1)
5QNF 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H9_1)
5QMW 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G2_1)
2B3S 2006-09-05 1.96 structure of the DSBA mutant (P31G-C33A)
5QLW 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D3_1)
5QLB 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C10_1)
5QM1 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D8_1)
5QM8 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E3_1)
5QM9 2020-02-05 1.96 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E4_1)
5QKW 2020-02-05 1.97 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B10_2)
5QLR 2020-02-05 1.97 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D10_1)
5QO7 2020-02-05 1.97 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_37)
5QNI 2020-02-05 1.97 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_1)
5QMD 2020-02-05 1.97 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E9_1)
5QMN 2020-02-05 1.98 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F5_1)
5QOA 2020-02-05 1.98 Group deposition of apo datasets for PANDDA analysis - Crystal Structure of apo EcDsbA after initial refinement (apo_dataset_40)
5QM3 2020-02-05 1.98 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E1_1)
5QLK 2020-02-05 1.99 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C7_1)
6WHD 2020-06-24 1.99 Crystal structure of E.coli DsbA in complex with diaryl ether analogue 2
6PVZ 2019-12-25 1.99 E.coli DsbA in complex with benzofuran compound 28 ((6-benzyl-1-benzofuran-3-yl)acetic acid)
6BR4 2017-12-27 1.99 Crystal structure of Escherichia coli DsbA in complex with {N}-methyl-1-(3-thiophen-2-ylphenyl)methanamine
6BQX 2017-12-27 1.99 Crystal structure of Escherichia coli DsbA in complex with N-methyl-1-(4-phenoxyphenyl)methanamine
5QMR 2020-02-05 2.0 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F9_1)
1DSB 1994-01-31 2.0 CRYSTAL STRUCTURE OF THE DSBA PROTEIN REQUIRED FOR DISULPHIDE BOND FORMATION IN VIVO
5QKO 2020-02-05 2.0 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A6_1)
6PML 2019-11-06 2.0 Crystal Structure of EcDsbA in complex benzyl ether 23
5QLZ 2020-02-05 2.0 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D6_1)
1A2J 1998-09-16 2.0 OXIDIZED DSBA CRYSTAL FORM II
1U3A 2005-05-03 2.0 mutant DsbA
5QLF 2020-02-05 2.0 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C3_2)
1AC1 1997-10-15 2.0 DSBA MUTANT H32L
5QM4 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E10_1)
5QMF 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F1_1)
5QN0 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G6_2)
5QKX 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B10_1)
5QL4 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B6_1)
6PG1 2020-07-01 2.01 Crystal Structure of EcDsbA in a complex with unpurified reaction product F1 (methylpiperazinone 6)
5QLS 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D11_1)
5QMP 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F7_1)
5QM5 2020-02-05 2.01 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E11_1)
5QMU 2020-02-05 2.04 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G11_1)
5QMT 2020-02-05 2.04 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G10_1)
5QM7 2020-02-05 2.04 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E2_1)
5QLI 2020-02-05 2.04 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C5_1)
5QL0 2020-02-05 2.05 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B2_1)
5QMS 2020-02-05 2.05 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G1_1)
5QMO 2020-02-05 2.05 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F6_1)
1FVJ 1997-05-15 2.06 THE 2.06 ANGSTROM STRUCTURE OF THE H32Y MUTANT OF THE DISULFIDE BOND FORMATION PROTEIN (DSBA)
5QLM 2020-02-05 2.07 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C9_1)
5QKK 2020-02-05 2.08 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A4_2)
5QMH 2020-02-05 2.08 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F11_1)
5QMZ 2020-02-05 2.08 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G5_1)
5QL6 2020-02-05 2.08 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B7_1)
6PIQ 2020-07-01 2.1 Crystal Structure of EcDsbA in a complex with unpurified reaction product G6 (pyrazole 9)
5QMG 2020-02-05 2.11 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F10_1)
5QKD 2020-02-05 2.11 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A10_1)
5QL2 2020-02-05 2.11 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B4_1)
5QKE 2020-02-05 2.12 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A11_1)
5QKS 2020-02-05 2.12 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A8_1)
5QLD 2020-02-05 2.12 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C12_1)
5QKJ 2020-02-05 2.13 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A3_1)
5QL1 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B3_1)
5QKV 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B1_2)
5QKF 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A12_1)
5QN3 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G7_1)
5QMA 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E5_1)
5QNA 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H4_1)
5QL5 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B6_2)
5QN7 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H1_1)
5QNC 2020-02-05 2.14 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H6_1)
5QL7 2020-02-05 2.15 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B8_1)
5QKC 2020-02-05 2.15 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A1_1)
5QL9 2020-02-05 2.15 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B9_2)
5QLY 2020-02-05 2.17 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D5_1)
5QML 2020-02-05 2.19 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F3_1)
5QN1 2020-02-05 2.2 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G6_3)
5QLU 2020-02-05 2.2 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D2_2)
5QLC 2020-02-05 2.21 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C11_1)
5QNH 2020-02-05 2.21 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure Phaux_1)
5QMY 2020-02-05 2.22 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G4_1)
5QM6 2020-02-05 2.3 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure E12_1)
6PC9 2020-05-20 2.3 Crystal Structure of EcDsbA in a complex with purified methylpiperazinone 6
5QMJ 2020-02-05 2.3 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure F12_1)
5QKI 2020-02-05 2.3 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A2_2)
5QN2 2020-02-05 2.3 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G6_1)
5QL8 2020-02-05 2.3 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B9_1)
5QKU 2020-02-05 2.31 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B1_1)
5QLV 2020-02-05 2.34 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D2_1)
5QLO 2020-02-05 2.37 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D1_3)
5QN4 2020-02-05 2.37 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure G8_1)
5QKZ 2020-02-05 2.38 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B12_2)
5QNE 2020-02-05 2.39 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure H7_1)
1UN2 2003-09-26 2.4 Crystal structure of circularly permuted CPDSBA_Q100T99: Preserved Global Fold and Local Structural Adjustments
5QLT 2020-02-05 2.4 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D12_1)
5QLN 2020-02-05 2.41 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure COMU_1)
5QKQ 2020-02-05 2.42 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A7_1)
5QKY 2020-02-05 2.42 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure B12_1)
5QLG 2020-02-05 2.48 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure C3_1)
5QKM 2020-02-05 2.49 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A5_1)
5QKR 2020-02-05 2.5 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A7_2)
4TKY 2015-01-14 2.5 The complex structure of E. coli DsbA bound to a peptide at the DsbA/DsbB interface
5QLP 2020-02-05 2.52 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure D1_1)
1TI1 2005-05-03 2.6 crystal structure of a mutant DsbA
2B6M 2006-10-17 2.65 Structure of the DsbA mutant (P31A-C33A)
5QKH 2020-02-05 2.65 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A2_1)
5QKL 2020-02-05 2.7 Group deposition of library data - Crystal Structure of EcDsbA after initial refinement with no ligand modelled (structure A4_1)
1A2M 1998-07-08 2.7 OXIDIZED DSBA AT 2.7 ANGSTROMS RESOLUTION, CRYSTAL FORM III
1A2L 1998-07-08 2.7 REDUCED DSBA AT 2.7 ANGSTROMS RESOLUTION
1BQ7 1999-08-20 2.8 DSBA MUTANT P151A, ROLE OF THE CIS-PROLINE IN THE ACTIVE SITE OF DSBA
2HI7 2006-12-05 3.7 Crystal structure of DsbA-DsbB-ubiquinone complex
3E9J 2008-11-25 3.7 Structure of the charge-transfer intermediate of the transmembrane redox catalyst DsbB
2ZUP 2009-04-14 3.7 Updated crystal structure of DsbB-DsbA complex from E. coli

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.5 Thiol:disulfide interchange protein DsbA P52235 DSBA_SHIFL
99.5 Thiol:disulfide interchange protein DsbA P0A4L5 DSBA_ECOL6
99.5 Thiol:disulfide interchange protein DsbA P0A4L6 DSBA_ECO27
100.0 Thiol:disulfide interchange protein DsbA P0AEG4 DSBA_ECOLI
100.0 Thiol:disulfide interchange protein DsbA P0AEG5 DSBA_ECO57