Thermodynamic stability of ribonuclease A (6.2 mM pH 1.0, 0.15 M KCl, in 2H2O) has been studied in the pressure range of 1 to 2000 atm and in the temperature range of 7.5 to 40 degrees C with a high pressure 1H NMR technique at 400 MHz. His epsilon proton resonances were used as reporter groups to measure fractions of folded and unfolded species. Gibbs energy differences between folded and unfolded species were obtained as functions of pressure for different temperatures and as functions of temperature for different pressures. The volume increase upon unfolding, delta V, was negative and temperature-dependent, decreasing from -10 ml/mol at 7.5 degrees C to -30 ml/mol at 37 degrees C. From the least squares-fitting of experimental Gibbs energy differences to a theoretical expression holding pressure and delta Cp constant, we determined best-fit values of delta G, delta H, delta S and delta Cp for different values of pressure in the temperature range 7.5 to 40 degrees C. We found that delta Cp is dependent on pressure, decreasing from 1.79 kcal/mol K at 1 atm to 1.08 kcal/mol K at 2000 atm. These findings appear to be consistent with a notion that the state of hydration of non-polar side-chains upon unfolding of the protein is a major factor that determines the pressure dependence of the conformational stability of ribonuclease A under the chosen experimental condition. Study holds ProTherm entries: 7490 Extra Details: 1. thermodynamic parameters for other pressures are also available,2. water was added in the experiment protein unfolding; thermodynamics; high pressure; NMR;,ribonuclease A
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:34 p.m.
|Number of data points||3|
|Proteins||Ribonuclease pancreatic ; Ribonuclease pancreatic|
|Assays/Quantities/Protocols||Experimental Assay: dCp ; Experimental Assay: Tm ; Experimental Assay: dHvH|
|Libraries||Mutations for sequence KETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV|
|Percent Identity||Matching Chains||Protein||Accession||Entry Name|