The human RAD52 protein plays an important role in the earliest stages of chromosomal double-strand break repair via the homologous recombination pathway. Individual subunits of RAD52 associate into seven-membered rings. These rings can form higher order complexes. RAD52 binds to DNA breaks, and recent studies suggest that the higher order self-association of the rings promotes DNA end joining. Monomers of the RAD52(1--192) deletion mutant also associate into ring structures but do not form higher order complexes. The thermal stability of wild-type and mutant RAD52 was studied by differential scanning calorimetry. Three thermal transitions (labeled A, B, and C) were observed with melting temperatures of 38.8, 73.1, and 115.2 degrees C. The RAD52(1--192) mutant had only two thermal transitions at 47.6 and 100.9 degrees C (labeled B and C). Transitions were labeled such that transition C corresponds to complete unfolding of the protein. The effect of temperature and protein concentration on RAD52 self-association was analyzed by dynamic light scattering. From these data a four-state hypothetical model was developed to explain the thermal denaturation profile of wild-type RAD52. The three thermal transitions in this model were assigned as follows. Transition A was attributed to the disruption of higher order assemblies of RAD52 rings, transition B to the disruption of rings to individual subunits, and transition C to complete unfolding. The ring-shaped quaternary structure of RAD52 and the formation of higher ordered complexes of rings appear to contribute to the extreme stability of RAD52. Higher ordered complexes of rings are stable at physiological temperatures in vitro. Study holds ProTherm entries: 11463, 11464, 11465, 11466, 11467, 11468, 11469, 11470, 11471 Extra Details: Transition 2 chromosomal double-strand break repair; homologous recombination pathway;,self-association; ring structures; dynamic light scattering
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:42 p.m.
|Number of data points||9|
|Proteins||DNA repair protein RAD52 homolog ; DNA repair protein RAD52 homolog|
|Assays/Quantities/Protocols||Experimental Assay: Tm prot_conc:3.1 mg/mL ; Experimental Assay: Tm prot_conc:7.2 mg/mL ; Experimental Assay: Tm prot_conc:3.5 mg/mL ; Experimental Assay: Tm prot_conc:2.0 mg/mL|
|Libraries||Mutations for sequence MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCR|