Thermal denaturation of T4 gene 32 protein: effects of zinc removal and substitution.


Abstract

Gene 32 protein (g32P), the single-stranded (ss) DNA binding protein from bacteriophage T4, is a zinc metalloprotein. The intrinsic zinc is one of the factors required for the protein to bind cooperatively to a ssDNA lattice. We have used differential scanning calorimetry to determine how the thermodynamic parameters characterizing the denaturation of g32P are affected by removal or substitution of the intrinsic zinc. Over a wide concentration range (1-10 mg/mL), the native Zn(II) protein unfolds at a tm of 55 degrees C with an associated mean enthalpy change of 139 kcal mol-1. Under the same conditions, the metal-free apoprotein denatures over a relatively broader temperature range centered at 49 degrees C, with a mean enthalpy change of 84 kcal mol-1. Substitution of Zn(II) in g32P by either Cd(II) or Co(II) does not significantly change the enthalpy of denaturation but does affect the thermal stability of the protein. All metallo forms of g32P when bound to poly(dT) undergo highly cooperative denaturational transitions characterized by asymmetric differential scanning calorimetry peaks with increases in tm of 4-5 degrees C compared to the unliganded metalloprotein. Removal of the metal ion from g32P significantly reduces the cooperativity of binding to poly(dT) [Giedroc, D. P., Keating, K. M., Williams, K. R., & Coleman, J. E. (1987) Biochemistry 26, 5251-5259], and presumably as a consequence of this, apo-g32P shows no change in either the shape or the midpoint of the thermal transition on binding to poly(dT).(ABSTRACT TRUNCATED AT 250 WORDS) Study holds ProTherm entries: 3506, 3507, 3508, 3509 Extra Details: gene 32 protein; differential scanning calorimetry;,thermal stability; enthalpy change

Submission Details

ID: T37uZsj93

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:21 p.m.

Version: 1

Publication Details
Keating KM;Ghosaini LR;Giedroc DP;Williams KR;Coleman JE;Sturtevant JM,Biochemistry (1988) Thermal denaturation of T4 gene 32 protein: effects of zinc removal and substitution. PMID:3262371
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
5JTN 2016-08-24 The structure of chaperone SecB in complex with unstructured proPhoA binding site c
5JTM 2016-08-24 The structure of chaperone SecB in complex with unstructured PhoA binding site a
5JTL 2016-08-24 The structure of chaperone SecB in complex with unstructured proPhoA
2MLZ 2014-05-21 NMR structure of E. coli Trigger Factor in complex with unfolded PhoA365-471
5JTO 2016-08-24 The structure of chaperone SecB in complex with unstructured proPhoA binding site d
5JTP 2016-08-24 The structure of chaperone SecB in complex with unstructured proPhoA binding site e
3FSA 2009-03-10 0.98 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM); chemically reduced.
3FS9 2009-03-10 1.05 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM)
1Q0E 2003-09-23 1.15 Atomic resolution (1.15 ) crystal structure of bovine copper, zinc superoxide dismutase
3U25 2011-12-28 1.18 Crystal structure of P. aeruginoas azurin containing a Tyr-His hydrogen bonded pair
3TG0 2011-11-09 1.2 E. coli alkaline phosphatase with bound inorganic phosphate
2FT6 2006-04-11 1.25 Structure of Cu(II)azurin with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CTPHPM'
3N2J 2011-04-06 1.35 Azurin H117G, oxidized form
2I7S 2006-12-05 1.35 Crystal structure of Re(phen)(CO)3 (Thr124His)(His83Gln) Azurin Cu(II) from Pseudomonas aeruginosa
2FT7 2006-04-11 1.4 Structure of Cu(I)azurin at pH 6, with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CTPHPM'
3BDG 2008-11-25 1.4 Crystal structure of wild-type/T155V mixed dimer of E. coli alkaline phosphatase
3BDF 2008-11-25 1.4 Crystal structure of metal-free E. coli alkaline phosphatase (T155V)
1JZG 2001-10-17 1.4 Pseudomonas aeruginosa Reduced Azurin (Cu1+) Ru(tpy)(phen)(His83)
2FNW 2006-10-03 1.4 Pseudomonas aeruginosa E2Q/H83Q/M109H-azurin RE(PHEN)(CO)3
3IBO 2009-12-08 1.45 Pseudomonas aeruginosa E2Q/H83Q/T126H-azurin RE(PHEN)(CO)3
2ZOW 2009-06-30 1.45 Crystal Structure of H2O2 treated Cu,Zn-SOD
4KOC 2014-05-14 1.46 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I/Y108F)
4BWW 2014-06-11 1.48 Crystal structure of spin labelled azurin T21R1.
1XB3 2004-10-19 1.5 The D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin
1JZF 2001-10-17 1.5 Pseudomonas aeruginosa Oxidized Azurin(Cu2+) Ru(tpy)(phen)(His83)
2I7O 2007-08-14 1.5 Structure of Re(4,7-dimethyl-phen)(Thr124His)(Lys122Trp)(His83Gln)AzCu(II), a Rhenium modified Azurin mutant
4JKN 2014-02-19 1.54 Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A
4MFH 2014-01-15 1.54 Crystal Structure of M121G Azurin
2FT8 2006-04-11 1.55 Structure of Cu(I)azurin, pH8, with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CTPHPM'
2Z7Y 2008-09-02 1.55 Crystal Structure of H2O2 treated Cu,Zn-SOD
2HX7 2007-01-30 1.55 Crystal structure of Cu(II) Azurin with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CSPHQGAGM'
1Y6V 2005-06-21 1.6 Structure of E. coli Alkaline Phosphatase in presence of cobalt at 1.60 A resolution
2HX8 2007-01-30 1.6 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CSPHQGAGM', at pH5
2XV0 2010-12-29 1.6 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM), chemically reduced, pH4.8
4HHG 2012-11-21 1.6 Crystal structure of the Pseudomonas aeruginosa azurin, RuH107NO YOH109
2XV2 2010-12-29 1.6 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM), chemically reduced, pH4.2
6GYI 2019-01-30 1.6 Azurin fom Pseudomonas aeruginosa treated with hydrosulfide
2GHZ 2006-07-04 1.6 Crystal structure of Azurin Phe114Pro mutant
1JZE 2001-10-17 1.6 Pseudomonas aeruginosa Azurin Ru(bpy)2(im)(His83)
2FTA 2006-04-11 1.61 Structure of Cu(II)azurin with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CTPHPFM'
1JZI 2001-10-17 1.62 Pseudomonas aeruginosa Azurin Re(phen)(CO)3(His83)
4QKT 2014-08-13 1.64 Azurin mutant M121EM44K with copper
1CBJ 1999-03-03 1.65 CRYSTAL STRUCTURE OF BOVINE SUPEROXIDE DISMUTASE CRYSTAL.
1CC3 1999-12-23 1.65 PURPLE CUA CENTER
5YT7 2018-08-08 1.66 crystal structure of circularly permutated Azurin 3
2HX9 2007-01-30 1.7 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CSPHQGAGM', at pH4
2GI0 2006-07-04 1.7 Crystal structure of Cu(I) Phe114Pro Azurin mutant
3UGE 2012-11-07 1.7 Silver Metallated Pseudomonas aeruginosa Azurin at 1.70 A
1JZH 2001-10-17 1.7 Pseudomonas aeruginosa Azurin Ru(tpy)(bpy)(His83)
4K9J 2013-10-02 1.7 Structure of Re(CO)3(4,7-dimethyl-phen)(Thr126His)(Lys122Trp)(His83Glu)(Trp48Phe)(Tyr72Phe)(Tyr108Phe)AzCu(II), a Rhenium modified Azurin mutant
1E9P 2000-12-03 1.7 Crystal structure of bovine Cu, Zn SOD to 1.7 Angstrom (3 of 3)
4KO6 2014-05-14 1.74 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95K/Y108F)
1ED8 2000-09-20 1.75 STRUCTURE OF E. COLI ALKALINE PHOSPHATASE INHIBITED BY THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION
1E9Q 2000-12-03 1.75 Crystal structure of bovine Cu Zn SOD - (1 of 3)
1ED9 2000-09-20 1.75 STRUCTURE OF E. COLI ALKALINE PHOSPHATASE WITHOUT THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION
1Y7A 2005-06-21 1.77 Structure of D153H/K328W E. coli alkaline phosphatase in presence of cobalt at 1.77 A resolution
4KO5 2014-05-14 1.79 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48L/V95I/Y108F)
1I53 2001-10-17 1.8 RE(I)-TRICARBONLY DIIMINE (Q107H)) AZURIN
2AEO 2006-05-02 1.8 Crystal structure of cisplatinated bovine Cu,Zn superoxide dismutase
1GR7 2002-05-16 1.8 Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution
1JZJ 2001-10-24 1.8 Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83)
2Z7W 2008-09-02 1.8 Crystal Structure of H2O2 treated Cu,Zn-SOD
3JTB 2009-11-17 1.8 Cu(II) N47S/F114N variant of Pseudomonas Aeruginosa Azurin
3FT0 2009-03-10 1.8 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced
1XB6 2004-10-19 1.82 The K24R mutant of Pseudomonas Aeruginosa Azurin
1E9O 2000-12-03 1.85 Crystal structure of bovine SOD - 1 of 3
3BDH 2008-11-18 1.85 Crystal structure of zinc-deficient wild-type E. coli alkaline phosphatase
1E65 2000-08-16 1.85 Azurin from Pseudomonas aeruginosa, apo form
6MJS 2019-02-20 1.85 Azurin 122W/124W/126Re
2Z7Z 2008-09-02 1.85 Crystal Structure of H2O2 treated Cu,Zn-SOD
4KOB 2014-05-14 1.87 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I)
6MJT 2019-02-20 1.89 Azurin 122F/124W/126Re
5I26 2016-04-13 1.89 Azurin T30R1, crystal form I
5AZU 1994-01-31 1.9 CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP
2AZU 1993-07-15 1.9 X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35*GLN AND HIS35*LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA
1R1C 2003-09-30 1.9 PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)
1SXC 1995-06-03 1.9 CRYSTAL STRUCTURE OF REDUCED BOVINE ERYTHROCYTE SUPEROXIDE DISMUTASE AT 1.9 ANGSTROMS RESOLUTION
1SXN 1998-03-18 1.9 REDUCED BOVINE SUPEROXIDE DISMUTASE AT PH 5.0
1VLX 1997-03-12 1.9 STRUCTURE OF ELECTRON TRANSFER (COBALT-PROTEIN)
1SXA 1995-06-03 1.9 CRYSTAL STRUCTURE OF REDUCED BOVINE ERYTHROCYTE SUPEROXIDE DISMUTASE AT 1.9 ANGSTROMS RESOLUTION
4AZU 1994-01-31 1.9 CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP
3FQY 2009-11-10 1.9 Azurin C112D
3FQ1 2009-11-10 1.9 Azurin C112D/M121I
4HIP 2012-11-21 1.9 Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109
1B8J 1999-02-18 1.9 ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE
3FQ2 2009-11-10 1.91 Azurin C112D/M121F
4WKX 2015-10-07 1.94 Reversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with Red Copper Site
5I28 2016-04-13 1.95 Azurin T30R1, crystal form II
1EW9 2002-05-01 2.0 ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH MERCAPTOMETHYL PHOSPHONATE
6IAV 2019-01-30 2.0 CO-AZURIN FROM PSEUDOMONAS AERUGINOSA TREATED WITH HYDROSULFIDE
3FSW 2009-03-10 2.0 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAM)
2G9Y 2006-10-10 2.0 Structure of S102T E. coli alkaline phosphatase in presence of phosphate at 2.00 A resolution
1JVL 2002-01-04 2.0 Azurin dimer, covalently crosslinked through bis-maleimidomethylether
4QLW 2014-08-13 2.0 Azurin mutant M121E with iron
3FSZ 2009-03-10 2.0 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM)
1E5Y 2000-08-19 2.0 Azurin from Pseudomonas aeruginosa, reduced form, pH 5.5
3HW7 2010-06-23 2.0 High pressure (0.57 GPa) crystal structure of bovine copper, zinc superoxide dismutase at 2.0 angstroms
1XB8 2004-10-19 2.0 Zn substituted form of D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin
1KH5 2002-03-13 2.0 E. COLI ALKALINE PHOSPHATASE MUTANT (D330N) MIMIC OF THE TRANSITION STATES WITH ALUMINIUM FLUORIDE
1EZL 2000-08-09 2.0 CRYSTAL STRUCTURE OF THE DISULPHIDE BOND-DEFICIENT AZURIN MUTANT C3A/C26A: HOW IMPORTANT IS THE S-S BOND FOR FOLDING AND STABILITY?
1SXS 1998-09-30 2.0 Reduced bovine superoxide dismutase at pH 5.0 complexed with thiocyanate
1E5Z 2000-08-19 2.0 Azurin from Pseudomonas aeruginosa, reduced form, pH 9.0
2SOD 1980-05-07 2.0 DETERMINATION AND ANALYSIS OF THE 2 ANGSTROM STRUCTURE OF COPPER, ZINC SUPEROXIDE DISMUTASE
1SXB 1995-06-03 2.0 CRYSTAL STRUCTURE OF REDUCED BOVINE ERYTHROCYTE SUPEROXIDE DISMUTASE AT 1.9 ANGSTROMS RESOLUTION
4HHW 2012-11-21 2.0 Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122
1ALK 1994-01-31 2.0 REACTION MECHANISM OF ALKALINE PHOSPHATASE BASED ON CRYSTAL STRUCTURES. TWO METAL ION CATALYSIS
1COB 1993-10-31 2.0 CRYSTAL STRUCTURE SOLUTION AND REFINEMENT OF THE SEMISYNTHETIC COBALT SUBSTITUTED BOVINE ERYTHROCYTE ENZYME SUPEROXIDE DISMUTASE AT 2.0 ANGSTROMS RESOLUTION
6MJR 2019-02-20 2.01 Azurin 122W/124F/126Re
5C66 2016-06-01 2.03 E. Coli Alkaline Phosphatase in complex with tungstate
1URA 1996-07-11 2.04 ALKALINE PHOSPHATASE (D51ZN)
1SXZ 1998-09-30 2.05 Reduced bovine superoxide dismutase at pH 5.0 complexed with azide
3CMR 2008-07-29 2.05 E. coli alkaline phosphatase mutant R166S in complex with phosphate
4KO9 2014-05-14 2.05 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V95I/Y108F)
4KO7 2014-05-14 2.07 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48F/V95I)
3FPY 2009-11-10 2.1 Azurin C112D/M121L
2Z7U 2008-09-02 2.1 Crystal Structure of H2O2 treated Cu,Zn-SOD
3AZU 1993-07-15 2.1 X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA
3JT2 2009-11-17 2.1 Cu(II) N47S/M121L variant of Pseudomonas Aeruginosa azurin
3SOD 1993-04-15 2.1 CHANGES IN CRYSTALLOGRAPHIC STRUCTURE AND THERMOSTABILITY OF A CU,ZN SUPEROXIDE DISMUTASE MUTANT RESULTING FROM THE REMOVAL OF BURIED CYSTEINE
3NP3 2010-10-13 2.1 C112D/M121E Pseudomonas Aeruginosa Azurin
1E67 2000-08-16 2.14 Zn-Azurin from Pseudomonas aeruginosa
1URB 1996-07-11 2.14 ALKALINE PHOSPHATASE (N51MG)
1ILS 1996-03-08 2.2 X-RAY CRYSTAL STRUCTURE THE TWO SITE-SPECIFIC MUTANTS ILE7SER AND PHE110SER OF AZURIN FROM PSEUDOMONAS AERUGINOSA
2TSA 1996-11-08 2.2 AZURIN MUTANT M121A
1GPC 1995-10-15 2.2 CORE GP32, DNA-BINDING PROTEIN
1NZR 1995-02-27 2.2 CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION
1ALI 1995-11-14 2.2 ALKALINE PHOSPHATASE MUTANT (H412N)
1EW8 2002-05-01 2.2 ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH PHOSPHONOACETIC ACID
2GA3 2006-10-10 2.2 Structure of S102T E. coli Alkaline Phosphatase-phosphate intermediate at 2.20A resolution
4HZ1 2012-12-12 2.2 Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site.
2HXA 2007-01-30 2.21 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence 'CTFPGHSALM' replaced with 'CSPHQGAGM', at pH3.5
4YR1 2015-04-29 2.24 Crystal Structure of E. Coli Alkaline Phosphatase D101A/D153A in complex with inorganic phosphate
3NP4 2010-10-13 2.25 C112D/M121E Pseudomonas aeruginosa Azurin
2IDF 2008-03-18 2.25 P. aeruginosa azurin N42C/M64E double mutant, BMME-linked dimer
1HQA 1996-03-08 2.25 ALKALINE PHOSPHATASE (H412Q)
1HJK 1997-10-15 2.3 ALKALINE PHOSPHATASE MUTANT H331Q
1ANJ 1996-01-29 2.3 ALKALINE PHOSPHATASE (K328H)
1ILU 1996-03-08 2.3 X-RAY CRYSTAL STRUCTURE THE TWO SITE-SPECIFIC MUTANTS ILE7SER AND PHE110SER OF AZURIN FROM PSEUDOMONAS AERUGINOSA
2XV3 2010-12-29 2.3 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced, pH5.3
1BEX 1998-10-14 2.3 STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN
1KHJ 2002-03-13 2.3 E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) MIMIC OF THE TRANSITION STATES WITH ALUMINIUM FLUORIDE
3DYC 2008-10-21 2.3 Structure of E322Y Alkaline Phosphatase in Complex with Inorganic Phosphate
1ETJ 1997-04-21 2.3 AZURIN MUTANT WITH MET 121 REPLACED BY GLU
2OJ1 2007-11-27 2.3 Disulfide-linked dimer of azurin N42C/M64E double mutant
1CB4 1999-03-03 2.3 CRYSTAL STRUCTURE OF COPPER, ZINC SUPEROXIDE DISMUTASE
3DPC 2009-06-16 2.3 Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide
3FSV 2009-03-10 2.3 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAHAAAM)
2TSB 1996-11-08 2.3 AZURIN MUTANT M121A-AZIDE
3IN0 2009-10-27 2.35 Crystal structure of the F114P/M121Q variant of Pseudomonas aeruginosa azurin in the Cu(II) state
1KH7 2002-03-27 2.4 E. COLI ALKALINE PHOSPHATASE MUTANT (D153GD330N)
3OQR 2010-10-13 2.4 C112D/M121E Azurin, pH 10.0
1KH4 2002-03-27 2.4 E. COLI ALKALINE PHOSPHATASE MUTANT (D330N) IN COMPLEX WITH PHOSPHATE
1AZR 1993-07-15 2.4 CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA ZINC AZURIN MUTANT ASP47ASP AT 2.4 ANGSTROMS RESOLUTION
1AG0 1997-10-29 2.4 STRUCTURE OF CYS 112 ASP AZURIN FROM PSEUDOMONAS AERUGINOSA
2ANH 1996-01-29 2.4 ALKALINE PHOSPHATASE (D153H)
5SYD 2017-08-16 2.4 Circularly permutated azurin (cpAz) based on P. aeruginosa azurin sequence
5TPQ 2017-11-01 2.45 E. coli alkaline phosphatase D101A, D153A, R166S, E322A, K328A mutant
1AJA 1995-11-14 2.5 THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY
1AJC 1995-11-14 2.5 THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY
1ANI 1996-01-29 2.5 ALKALINE PHOSPHATASE (D153H, K328H)
1SDA 1993-10-31 2.5 CRYSTAL STRUCTURE OF PEROXYNITRITE-MODIFIED BOVINE CU,ZN SUPEROXIDE DISMUTASE
1KH9 2002-03-13 2.5 E. COLI ALKALINE PHOSPHATASE MUTANT (D153GD330N) COMPLEX WITH PHOSPHATE
1AJD 1995-11-14 2.5 THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY
1KHK 2002-03-13 2.5 E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)
1KHL 2002-03-13 2.5 E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) COMPLEX WITH PHOSPHATE
1AJB 1995-11-14 2.5 THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY
1ALH 1995-02-27 2.5 KINETICS AND CRYSTAL STRUCTURE OF A MUTANT E. COLI ALKALINE PHOSPHATASE (ASP-369-->ASN): A MECHANISM INVOLVING ONE ZINC PER ACTIVE SITE
1AZN 1994-10-15 2.6 CRYSTAL STRUCTURE OF THE AZURIN MUTANT PHE114ALA FROM PSEUDOMONAS AERUGINOSA AT 2.6 ANGSTROMS RESOLUTION
1ELX 1998-05-27 2.6 E. COLI ALKALINE PHOSPHATASE MUTANT (S102A)
3IN2 2009-11-17 2.6 Crystal structure of the N47S/M121L variant of Pseudomonas aeruginosa azurin in the Cu(II) state
1ALJ 1995-11-14 2.6 ALKALINE PHOSPHATASE MUTANT (H412N)
1KHN 2002-03-13 2.6 E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) ZINC FORM
1AZU 1980-09-26 2.7 STRUCTURAL FEATURES OF AZURIN AT 2.7 ANGSTROMS RESOLUTION
1JVO 2002-01-04 2.75 Azurin dimer, crosslinked via disulfide bridge
1ELY 1998-05-27 2.8 E. COLI ALKALINE PHOSPHATASE MUTANT (S102C)
4KM4 2013-07-24 2.8 E. coli alkaline phosphatase mutant S102G/R166S in complex with inorganic phosphate
1ELZ 1998-05-27 2.8 E. COLI ALKALINE PHOSPHATASE MUTANT (S102G)
2IWE 2007-06-12 2.83 Structure of a cavity mutant (H117G) of Pseudomonas aeruginosa azurin
5GAD 2016-01-27 3.7 RNC-SRP-SR complex early state
5GAG 2016-01-27 3.8 RNC in complex with SRP-SR in the closed state
5GAH 2016-01-27 3.8 RNC in complex with SRP with detached NG domain
5GAF 2016-02-03 4.3 RNC in complex with SRP

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Single-stranded DNA-binding protein O21959 VHED_BPR69
100.0 Single-stranded DNA-binding protein O21958 VHED_BPR32
100.0 Single-stranded DNA-binding protein O21957 VHED_BPR27
100.0 Single-stranded DNA-binding protein O21950 VHED_BPR03
100.0 Single-stranded DNA-binding protein O21960 VHED_BPS76
100.0 Single-stranded DNA-binding protein O21956 VHED_BPR18
100.0 Single-stranded DNA-binding protein O21955 VHED_BPR15
100.0 Single-stranded DNA-binding protein O21951 VHED_BPR06
100.0 Single-stranded DNA-binding protein O21954 VHED_BPR10
100.0 Single-stranded DNA-binding protein O21952 VHED_BPR08
100.0 Single-stranded DNA-binding protein O21949 VHED_BPPST
100.0 Single-stranded DNA-binding protein O21948 VHED_BPM1
100.0 Single-stranded DNA-binding protein O21947 VHED_BPFSA
100.0 Single-stranded DNA-binding protein O21953 VHED_BPR09
97.8 Single-stranded DNA-binding protein O21946 VHED_BPS14
97.9 Single-stranded DNA-binding protein O21945 VHED_BPR70
98.2 Single-stranded DNA-binding protein P09035 VHED_BPT2
99.1 Single-stranded DNA-binding protein P09797 VHED_BPT6
100.0 Single-stranded DNA-binding protein P03695 VHED_BPT4
99.2 Azurin B3EWN9 AZUR_PSEAI
100.0 Azurin P00282 AZUR_PSEAE
92.3 Alkaline phosphatase P21948 PPB_ESCF3
100.0 Alkaline phosphatase P00634 PPB_ECOLI
94.7 Superoxide dismutase [Cu-Zn] O46412 SODC_CEREL
97.4 Superoxide dismutase [Cu-Zn] P09670 SODC_SHEEP
98.0 Superoxide dismutase [Cu-Zn] Q5FB29 SODC_CAPHI
98.7 Superoxide dismutase [Cu-Zn] Q52RN5 SODC_BOSMU
100.0 Superoxide dismutase [Cu-Zn] P00442 SODC_BOVIN