A thermodynamic approach to the problem of stabilization of globular protein structure: a calorimetric study.


Abstract

Study holds ProTherm entries: 12342, 12343, 12344, 12345, 12346, 12347, 12348, 12349, 12350, 12351, 12352, 12353, 12354, 12355, 12356, 12357, 12358, 12359, 12360, 12361, 12362 Extra Details: Data taken from W.Pfeil (Protein stability and folding, Springer-Verlag 1998) page 384, Ref 74P1. two-state transition; intermediates; structual paramenters; hydrogen bond

Submission Details

ID: RvBFKsZy3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:43 p.m.

Version: 1

Publication Details
Privalov PL;Khechinashvili NN,J. Mol. Biol. (1974) A thermodynamic approach to the problem of stabilization of globular protein structure: a calorimetric study. PMID:4368360
Additional Information

Number of data points 56
Proteins Cytochrome c ; Myoglobin ; Ribonuclease pancreatic ; Chymotrypsinogen A ; Cytochrome c ; Lysozyme C ; Myoglobin ; Lysozyme C ; Ribonuclease pancreatic ; Calnexin
Unique complexes 5
Assays/Quantities/Protocols Experimental Assay: dCp pH:10.5 ; Experimental Assay: Tm pH:10.5 ; Experimental Assay: dHvH ; Experimental Assay: dCp pH:4.83 ; Experimental Assay: dHcal pH:4.83 ; Experimental Assay: Tm pH:4.83 ; Experimental Assay: dCp pH:4.52 ; Experimental Assay: dHcal pH:4.52 ; Experimental Assay: Tm pH:4.52 ; Experimental Assay: dCp pH:3.94 ; Experimental Assay: dHcal pH:3.94 ; Experimental Assay: Tm pH:3.94 ; Experimental Assay: dCp pH:3.75 ; Experimental Assay: dHcal pH:3.75 ; Experimental Assay: Tm pH:3.75 ; Experimental Assay: dCp pH:3.4 ; Experimental Assay: dHcal pH:3.4 ; Experimental Assay: Tm pH:3.4 ; Experimental Assay: dCp pH:3.18 ; Experimental Assay: dHcal pH:3.18 ; Experimental Assay: Tm pH:3.18 ; Experimental Assay: dCp pH:3.12 ; Experimental Assay: dHcal pH:3.12 ; Experimental Assay: Tm pH:3.12 ; Experimental Assay: dCp pH:3.05 ; Experimental Assay: dHcal pH:3.05 ; Experimental Assay: Tm pH:3.05 ; Experimental Assay: dCp pH:2.92 ; Experimental Assay: dHcal pH:2.92 ; Experimental Assay: Tm pH:2.92 ; Experimental Assay: dHcal pH:2.81 ; Experimental Assay: Tm pH:2.81 ; Experimental Assay: dCp pH:3.8 ; Experimental Assay: dHcal pH:3.8 ; Experimental Assay: Tm pH:3.8 ; Experimental Assay: dCp pH:3.3 ; Experimental Assay: dHcal pH:3.3 ; Experimental Assay: Tm pH:3.3 ; Experimental Assay: dCp pH:3.15 ; Experimental Assay: dHcal pH:3.15 ; Experimental Assay: Tm pH:3.15 ; Experimental Assay: dCp pH:2.82 ; Experimental Assay: dHcal pH:2.82 ; Experimental Assay: Tm pH:2.82 ; Experimental Assay: dCp pH:2.6 ; Experimental Assay: dHcal pH:2.6 ; Experimental Assay: Tm pH:2.6 ; Experimental Assay: dCp pH:2.44 ; Experimental Assay: dHcal pH:2.44 ; Experimental Assay: Tm pH:2.44 ; Experimental Assay: dCp pH:2.21 ; Experimental Assay: dHcal pH:2.21 ; Experimental Assay: Tm pH:2.21 ; Experimental Assay: dG_H2O temp:38.0 C, pH:4.7 ; Experimental Assay: dG_H2O temp:25.0 C, pH:7.0 ; Experimental Assay: dG_H2O pH:1.0, temp:25.0 C
Libraries Mutations for sequence SKSKPDTSAPTSPKVTYKAPVPSGEVYFADSFDRGTLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKETKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTPELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSIVNSGNLLNDMTPPVNPSREIEDPEDQKPEDWDERPKIPDPDAVKPDDWNEDAPAKIPDEEATKPDGWLDDEPEYVPDPDAEKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPMIDNPNYKGKWKPPMIDNPNYQGIWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIVCGDRRVVDDWANDGWGLKKAADGAAEP ; Mutations for sequence KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL ; Mutations for sequence VLSEGEWQLVLHVWAKVEADVAGHGQDILIRLFKSHPETLEKFDRFKHLKTEAEMKASEDLKKHGVTVLTALGAILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISEAIIHVLHSRHPGDFGADAQGAMNKALELFRKDIAAKYKELGYQG ; Mutations for sequence GDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITWGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE ; Mutations for sequence KETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV
Sequence Assay Result Units