Designing protein β-sheet surfaces by Z-score optimization


Studies of lattice models of proteins have suggested that the appropriate energy expression for protein design may include nonthermodynamic terms to accommodate negative design concerns. One method, developed in lattice model studies, maximizes a quantity known as the “Z-score,” which compares the lowest energy sequence whose ground state structure is the target structure to an ensemble of random sequences. Here we show that, in certain circumstances, the technique can be applied to real proteins. The resulting energy expression is used to design the β-sheet surfaces of two real proteins. We find experimentally that the designed proteins are stable and well folded, and in one case is even more thermostable than the wild type.

Submission Details


Submitter: Marie Ary

Submission Date: July 31, 2017, 11:46 a.m.

Version: 1

Publication Details
Street AG;Datta D;Gordon DB;Mayo SL,Phys Rev Lett (2000) Designing protein beta-sheet surfaces by Z-score optimization. PMID:10990854
Additional Information

Study Summary

Number of data points 1
Proteins Protein Gβ1
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: Tm
Libraries GMEC from Z-score optimization of B-sheet surface design (pos 4,6,15,17,42,44,53,55)

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)