Experimental library screening demonstrates the successful application of computational protein design to large structural ensembles.


Abstract

The stability, activity, and solubility of a protein sequence are determined by a delicate balance of molecular interactions in a variety of conformational states. Even so, most computational protein design methods model sequences in the context of a single native conformation. Simulations that model the native state as an ensemble have been mostly neglected due to the lack of suffi- ciently powerful optimization algorithms for multistate design. Here, we have applied our multistate design algorithm to study the potential utility of various forms of input structural data for design. To facilitate a more thorough analysis, we developed new methods for the design and high-throughput stability deter- mination of combinatorial mutation libraries based on protein design calculations. The application of these methods to the core design of a small model system produced many variants with improved thermodynamic stability and showed that multistate design methods can be readily applied to large structural ensem- bles. We found that exhaustive screening of our designed libraries helped to clarify several sources of simulation error that would have otherwise been difficult to ascertain. Interestingly, the lack of correlation between our simulated and experimentally mea- sured stability values shows clearly that a design procedure need not reproduce experimental data exactly to achieve success. This surprising result suggests potentially fruitful directions for the improvement of computational protein design technology.

Submission Details

ID: QjUzVQaT3

Submitter: Marie Ary

Submission Date: Feb. 15, 2017, 2:31 p.m.

Version: 1

Publication Details
Allen BD;Nisthal A;Mayo SL,Proc Natl Acad Sci U S A (2010) Experimental library screening demonstrates the successful application of computational protein design to large structural ensembles. PMID:21045132
Additional Information

Number of data points 83
Proteins Protein Gβ1
Unique complexes 63
Assays/Quantities/Protocols Experimental Assay: Cm
Libraries CPD of 10 core positions
Sequence Assay Result Units