Comparative study of mature and zymogen mite cysteine protease stability and pH unfolding.

Abstract

Papain-like proteases (CA1) are synthesized as inactive precursors carrying an N-terminal propeptide, which is further removed under acidic conditions to generate active enzymes. Study holds ProTherm entries: 25848, 25849, 25850, 25851, 25852 Extra Details: mature protease pH-induced unfolding; Propeptide; Der p 1; Cysteine protease; Stability; Mechanism of activation; Fluorescence quenching; Thermal denaturation; Allergen; Zymogen

Submission Details

ID: QbWe9iY

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:56 p.m.

Version: 1

Publication Details
Chevigné A;Dumez ME;Dumoulin M;Matagne A;Jacquet A;Galleni M,Biochim. Biophys. Acta (2010) Comparative study of mature and zymogen mite cysteine protease stability and pH unfolding. PMID:20682463
Additional Information

Study Summary

Number of data points 5
Proteins Der p 1 allergen ; Der p 1 allergen
Unique complexes 2
Assays/Quantities/Protocols Experimental Assay: Tm prot_conc:5 microM, pH:4.0 ; Experimental Assay: Tm prot_conc:5 microM, pH:7.0 ; Experimental Assay: Tm pH:3.5, prot_conc:1 microM ; Experimental Assay: Tm prot_conc:1 microM, pH:7.0
Libraries Mutations for sequence TNACSINGNAPAEIDLRQMRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRQQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQHNGVVQESYYRYVAREQSCRRPNAQRFGISNYCQIYPPNANKIREALAQTHSAIAVIIGIKDLDAFRHYDGRTIIQRDNGYQPNYHAVNIVGYSNAQGVDYWIVRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.3 Der p 1 allergen P08176 PEPT1_DERPT