Comparative study of mature and zymogen mite cysteine protease stability and pH unfolding.


Abstract

Papain-like proteases (CA1) are synthesized as inactive precursors carrying an N-terminal propeptide, which is further removed under acidic conditions to generate active enzymes. Study holds ProTherm entries: 25848, 25849, 25850, 25851, 25852 Extra Details: mature protease pH-induced unfolding; Propeptide; Der p 1; Cysteine protease; Stability; Mechanism of activation; Fluorescence quenching; Thermal denaturation; Allergen; Zymogen

Submission Details

ID: QbWe9iY

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:56 p.m.

Version: 1

Publication Details
Chevigné A;Dumez ME;Dumoulin M;Matagne A;Jacquet A;Galleni M,Biochim. Biophys. Acta (2010) Comparative study of mature and zymogen mite cysteine protease stability and pH unfolding. PMID:20682463
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1XKG 2005-06-28 1.61 Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution
3RVW 2012-01-11 1.95 Crystal structure of Der p 1 complexed with Fab 4C1
2AS8 2005-12-06 1.95 Crystal structure of mature and fully active Der p 1 allergen
5VPH 2017-05-24 2.5 CRYSTAL STRUCTURE OF DER P 1 COMPLEXED WITH FAB 4C1
3RVX 2012-01-11 2.5 Crystal structure of Der p 1 complexed with Fab 4C1
5VCO 2017-04-26 2.74 THE CRYSTAL STRUCTURE OF DER P 1 ALLERGEN COMPLEXED WITH FAB FRAGMENT OF MAB 10B9
4PP2 2015-04-08 2.74 The crystal structure of Der p 1 allergen complexed with Fab fragment of mAb 10B9
4PP1 2015-04-08 3.0 The crystal structure of Der p 1 allergen complexed with Fab fragment of mAb 5H8
5VCN 2017-04-26 3.0 THE CRYSTAL STRUCTURE OF DER P 1 ALLERGEN COMPLEXED WITH FAB FRAGMENT OF MAB 5H8

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.3 Der p 1 allergen P08176 PEPT1_DERPT