Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have investigated, using fluorescence and circular dichroism spectroscopy, the unfolding process of human fibronectin induced by urea in different ionic strength conditions. At any ionic strength, the equilibrium unfolding data are well described by a four-state equilibrium model N <= => I(1) <= =>I(2) <= => U. Fitting this model to experimental values, we have determined the free energy change for the different steps. We found that the N <= => I(1) transition corresponds to a free energy of 10.5 +/- 0.4 kcal/mol. Comparable values of free energy change are generally associated with a partial unfolding of the type III domain. For the I(1) <= => I(2) transition, the free energy change is 7.6 +/- 0.4 kcal/mol at low ionic strength but is twice as low at high ionic strength. This result is consistent with observations indicating that the complete unfolding of the type III domain from partially unfolded forms necessitates about 5 kcal/mol. The third step, I(2) <= => U, which leads to the complete unfolding of fibronectin, corresponds to a free energy change of 14.4 +/- 0.9 kcal/mol at low ionic strength whereas this energy is again twice as low under high ionic strength conditions. This hierarchical unfolding of fibronectin, as well as the stability of the different intermediates controlled by ionic strength demonstrated here, could be important for the understanding of activation of the matrix assembly. Study holds ProTherm entries: 16863, 16864, 16865, 16866, 16867, 16868, 16869, 16870, 16871, 16872, 16873, 16874, 16875, 16876, 16877 Extra Details: Transition 1. Experiment was done in pure water, 18 M ohm. structural changes; binding sites; intermolecular interactions; partial unfolding; ionic strength
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:48 p.m.
|Number of data points||30|
|Proteins||Fibronectin ; Fibronectin|
|Assays/Quantities/Protocols||Experimental Assay: m buffers:: ; Experimental Assay: dG_H2O buffers:: ; Experimental Assay: m ionic:NaCl: 150 mM, buffers:Tris-HCl: 10 mM ; Experimental Assay: dG_H2O ionic:NaCl: 150 mM, buffers:Tris-HCl: 10 mM ; Experimental Assay: m buffers:Tris-HCl: 10 mM, ionic:NaCl: 30 mM ; Experimental Assay: dG_H2O buffers:Tris-HCl: 10 mM, ionic:NaCl: 30 mM ; Experimental Assay: m buffers:Tris-HCl: 10 mM ; Experimental Assay: dG_H2O buffers:Tris-HCl: 10 mM ; Experimental Assay: m buffers:Tris-HCl: 1 mM ; Experimental Assay: dG_H2O buffers:Tris-HCl: 1 mM|
|Libraries||Mutations for sequence VSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRT|