The energetics of thermal denaturation of two isoforms of ribonuclease T1 (Gln25 and Lys25) in various solvents have been studied by differential scanning calorimetry. It has been shown that the thermal transition of both forms of RNase T1 is strongly affected by slow kinetics, which cause an apparent deviation of the transition from a simple two-state model. By decreasing the heating rate or increasing the transition temperature, the denaturation of RNase approaches an equilibrium two-state transition. This permits determination of the thermodynamic parameters characterizing unfolding of the native structure. These thermodynamic parameters were correlated with the structural features of protein. Analysis of different contributions to the stability of RNase T1 shows that van der Waals interactions and hydrogen bonding are the major contributors to the conformational stability of the protein. Study holds ProTherm entries: 2875, 2876, 2877, 2878, 2879, 2880, 2881, 2882, 2883, 2884, 2885, 2886 Extra Details: Ribonuclease T1; conformational stability; hydrogen bonding;,energetics; thermodynamic parameters
ID: MzBMLcYT
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:20 p.m.
Version: 1
Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Structure ID | Release Date | Resolution | Structure Title |
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1B2M | 1998-11-27T00:00:00+0000 | 2.0 | THREE-DIMENSIONAL STRUCTURE OF RIBONULCEASE T1 COMPLEXED WITH AN ISOSTERIC PHOSPHONATE ANALOGUE OF GPU: ALTERNATE SUBSTRATE BINDING MODES AND CATALYSIS. |
1BIR | 1996-01-04T00:00:00+0000 | 1.8 | RIBONUCLEASE T1, PHE 100 TO ALA MUTANT COMPLEXED WITH 2' GMP |
1BU4 | 1998-09-11T00:00:00+0000 | 1.9 | RIBONUCLEASE 1 COMPLEX WITH 2'GMP |
1BVI | 1998-09-15T00:00:00+0000 | 1.9 | RIBONUCLEASE T1 (WILDTYPE) COMPLEXED WITH 2'GMP |
1CH0 | 1999-03-30T00:00:00+0000 | 2.3 | RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT |
1DET | 1996-02-20T00:00:00+0000 | 1.8 | RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP |
1FYS | 2000-10-03T00:00:00+0000 | 2.0 | Ribonuclease T1 V16C mutant |
1FZU | 2000-10-04T00:00:00+0000 | 1.8 | RNAse T1 V78A mutant |
1G02 | 2000-10-05T00:00:00+0000 | 1.86 | Ribonuclease T1 V16S mutant |
1GSP | 1997-11-28T00:00:00+0000 | 2.2 | RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY |
Percent Identity | Matching Chains | Protein | Accession | Entry Name |
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100.0 | Guanyl-specific ribonuclease T1 | P00651 | RNT1_ASPOR |