Phenylglyoxal inactivates Escherichia coli adenylate kinase by modifying a single arginine residue (Arg-88). ATP, ADP, P1,P5-di(adenosine 5')-pentaphosphate, and to a lesser extent AMP protect the enzyme against inactivation by phenylglyoxal. Site-directed mutagenesis of Arg-88 to glycine yields a modified form of adenylate kinase (RG88 mutant) closely related structurally to the wild-type protein as indicated by Fourier transform infrared spectroscopy, differential scanning calorimetry, and limited proteolysis. However, this modified protein has only 1% of the maximum catalytic activity of the wild-type enzyme and 5- and 85-fold higher apparent Km values for ATP and AMP, respectively, than the parent adenylate kinase. Arg-88, which is a highly conserved residue in all known molecular forms of adenylate kinases (corresponding to Arg-97 in muscle cytosolic enzyme), should be located inside a big cleft of the molecule, close to the phosphate-binding loop. It possibly stabilizes the transferable gamma-phosphate group from ATP to AMP in the transition state. Study holds ProTherm entries: 3114, 3115, 14157 Extra Details: Escherichia coli adenylate kinase; catalytic role; arginine;,site-directed mutagenesis; structural
ID: Mohf28qL3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:21 p.m.
Version: 1
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Percent Identity | Matching Chains | Protein | Accession | Entry Name |
---|---|---|---|---|
100.0 | Adenylate kinase | A7ZIN4 | KAD_ECO24 | |
100.0 | Adenylate kinase | B7L799 | KAD_ECO55 | |
100.0 | Adenylate kinase | P69442 | KAD_ECO57 | |
100.0 | Adenylate kinase | C4ZUS8 | KAD_ECOBW | |
100.0 | Adenylate kinase | B1XFR1 | KAD_ECODH | |
100.0 | Adenylate kinase | A7ZXD2 | KAD_ECOHS | |
100.0 | Adenylate kinase | B1IZC0 | KAD_ECOLC | |
100.0 | Adenylate kinase | P69441 | KAD_ECOLI | |
100.0 | Adenylate kinase | B7N927 | KAD_ECOLU | |
100.0 | Adenylate kinase | B6I0C6 | KAD_ECOSE | |
100.0 | Adenylate kinase | B1LJN2 | KAD_ECOSM | |
100.0 | Adenylate kinase | B7LV13 | KAD_ESCF3 | |
100.0 | Adenylate kinase | B2U4S7 | KAD_SHIB3 | |
100.0 | Adenylate kinase | Q325C2 | KAD_SHIBS | |
100.0 | Adenylate kinase | Q0T7B1 | KAD_SHIF8 | |
99.5 | Adenylate kinase | B7M3W6 | KAD_ECO8A | |
99.5 | Adenylate kinase | Q32J54 | KAD_SHIDS | |
99.5 | Adenylate kinase | B7UKF4 | KAD_ECO27 | |
99.5 | Adenylate kinase | B7NIF6 | KAD_ECO7I | |
99.5 | Adenylate kinase | B7MQI9 | KAD_ECO81 | |
99.5 | Adenylate kinase | Q0TKG7 | KAD_ECOL5 | |
99.5 | Adenylate kinase | Q8FK84 | KAD_ECOL6 | |
99.5 | Adenylate kinase | Q83M40 | KAD_SHIFL | |
99.1 | Adenylate kinase | Q3Z4S5 | KAD_SHISS | |
99.1 | Adenylate kinase | B7MDZ6 | KAD_ECO45 | |
96.3 | Adenylate kinase | B5Y0N3 | KAD_KLEP3 | |
96.3 | Adenylate kinase | A6T5N7 | KAD_KLEP7 | |
95.8 | Adenylate kinase | A4W7F8 | KAD_ENT38 | |
96.3 | Adenylate kinase | B5EXN0 | KAD_SALA4 | |
96.3 | Adenylate kinase | Q57S76 | KAD_SALCH | |
96.3 | Adenylate kinase | B5FLJ7 | KAD_SALDC | |
96.3 | Adenylate kinase | B5QU77 | KAD_SALEP | |
96.3 | Adenylate kinase | B5R612 | KAD_SALG2 | |
96.3 | Adenylate kinase | B4T9I1 | KAD_SALHS | |
96.3 | Adenylate kinase | B4SWY1 | KAD_SALNS | |
96.3 | Adenylate kinase | Q5PFK8 | KAD_SALPA | |
96.3 | Adenylate kinase | C0Q812 | KAD_SALPC | |
96.3 | Adenylate kinase | B5BD44 | KAD_SALPK | |
96.3 | Adenylate kinase | B4TMG6 | KAD_SALSV | |
96.3 | Adenylate kinase | P0A1V5 | KAD_SALTI | |
96.3 | Adenylate kinase | P0A1V4 | KAD_SALTY | |
95.8 | Adenylate kinase | A8AJW9 | KAD_CITK8 | |
92.1 | Adenylate kinase | A8GAV3 | KAD_SERP5 |