Conformational properties of streptokinase--differential scanning calorimetric investigations.


Abstract

The two-domain structure of streptokinase (Sk) was demonstrated by scanning calorimetric investigations at neutral pH and low ionic strength. The melting pattern of the protein is composed of two two-state transitions at TtrS1 = 45.9 +/- 0.4 degrees C with delta H1 = 431 +/- 18 kJ/mol, and TtrS2 = 60.1 +/- 1.3 degrees C with delta H2 = 306 +/- 16 kJ/mol. The partial specific heat capacity of native Sk was determined to be Cp = 1.42 +/- 0.17 J/K/g and the denaturational heat capacity change associated with the two transitions, delta Cp1 = 0.21 J/K/g and delta Cp2 = 0.38 J/K/g, respectively. The overall melting pattern of Sk remains almost unchanged at a variety of tested solvent compositions, except at pH 4 (and below) and in the presence of denaturants. The two domains show different susceptibility to urea. It is proposed that the less thermostable domain is located within the N-terminal part (residues 1-230), and the more thermostable one, within the C-terminal region. Study holds ProTherm entries: 9791, 9792, 9793 Extra Details: transition 1 streptokinase; domain structure; calorimetry

Submission Details

ID: MXyntJt53

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:38 p.m.

Version: 1

Publication Details
Welfle K;Pfeil W;Misselwitz R;Welfle H;Gerlach D,Int. J. Biol. Macromol. (1992) Conformational properties of streptokinase--differential scanning calorimetric investigations. PMID:1596467
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1L4D 2002-12-11 2.3 CRYSTAL STRUCTURE OF MICROPLASMINOGEN-STREPTOKINASE ALPHA DOMAIN COMPLEX
1QQR 1999-06-17 2.3 CRYSTAL STRUCTURE OF STREPTOKINASE DOMAIN B
1L4Z 2002-12-11 2.8 X-RAY CRYSTAL STRUCTURE OF THE COMPLEX OF MICROPLASMINOGEN WITH ALPHA DOMAIN OF STREPTOKINASE IN THE PRESENCE CADMIUM IONS
1BML 1999-08-02 2.9 COMPLEX OF THE CATALYTIC DOMAIN OF HUMAN PLASMIN AND STREPTOKINASE

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
98.2 Streptokinase C P10519 STRP_STRS1
100.0 Streptokinase C P00779 STRP_STREQ
90.6 Streptokinase C P10520 STRP_STRP1