Factors effecting the thermostability of cysteine proteinases from Carica papaya.


Abstract

Thermal denaturation of four Carica papaya cysteine proteinases (papain, chymopapain, papaya proteinases 3 and 4) was studied as a function of pH using high-sensitivity differential scanning calorimetry. The ratios of calorimetric enthalpy to Van't Hoff enthalpy suggest that, for all these proteins, denaturation occurs as a non two state process, via an intermediate structure. Differences in the thermal stabilities of the proteinases; chymopapain > papaya proteinase 3 > papain > papaya proteinase 4, were correlated to their amino acid sequence to explain the observations in terms of mobility and specific residue mutation. Three-dimensional structures of papain and papaya proteinase 3 were similarly used to illustrate the influence of atomic mobility on stability. Study holds ProTherm entries: 7577, 7578, 7579, 7580, 7581, 7582, 7583, 7584, 7585, 7586, 7587, 7588, 7589, 7590, 7591, 7592, 7593, 7594, 7595, 7596 Extra Details:

Submission Details

ID: MWVJ5Cea3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:34 p.m.

Version: 1

Publication Details
Sumner IG;Harris GW;Taylor MA;Pickersgill RW;Owen AJ;Goodenough PW,Eur. J. Biochem. (1993) Factors effecting the thermostability of cysteine proteinases from Carica papaya. PMID:8508784
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2CIO 2006-05-18 1.5 The high resolution x-ray structure of papain complexed with fragments of the Trypanosoma brucei cysteine protease inhibitor ICP.
1KHQ 2003-09-09 1.6 ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX
1PPN 1994-01-31 1.6 STRUCTURE OF MONOCLINIC PAPAIN AT 1.60 ANGSTROMS RESOLUTION
9PAP 1986-10-24 1.65 STRUCTURE OF PAPAIN REFINED AT 1.65 ANGSTROMS RESOLUTION
1CVZ 2000-08-30 1.7 CRYSTAL STRUCTURE ANALYSIS OF PAPAIN WITH CLIK148(CATHEPSIN L SPECIFIC INHIBITOR)
1PIP 1993-10-31 1.7 CRYSTAL STRUCTURE OF PAPAIN-SUCCINYL-GLN-VAL-VAL-ALA-ALA-P-NITROANILIDE COMPLEX AT 1.7 ANGSTROMS RESOLUTION: NONCOVALENT BINDING MODE OF A COMMON SEQUENCE OF ENDOGENOUS THIOL PROTEASE INHIBITORS
1YAL 1996-12-23 1.7 CARICA PAPAYA CHYMOPAPAIN AT 1.7 ANGSTROMS RESOLUTION
3E1Z 2009-01-27 1.86 Crystal structure of the parasite protesase inhibitor chagasin in complex with papain
1PPP 1994-01-31 1.9 CRYSTAL STRUCTURE OF PAPAIN-E64-C COMPLEX. BINDING DIVERSITY OF E64-C TO PAPAIN S2 AND S3 SUBSITES
4QRV 2015-08-12 1.98 Crystal structure of I86F mutant of papain
1PPD 1985-01-02 2.0 RESTRAINED LEAST-SQUARES REFINEMENT OF THE SULFHYDRYL PROTEASE PAPAIN TO 2.0 ANGSTROMS
1KHP 2003-09-09 2.0 Monoclinic form of papain/ZLFG-DAM covalent complex
3IMA 2010-02-16 2.03 Complex strcuture of tarocystatin and papain
6H8T 2018-09-12 2.1 Crystal structure of Papain modify by achiral Ru(II)complex
1PE6 1993-04-15 2.1 REFINED X-RAY STRUCTURE OF PAPAIN(DOT)E-64-C COMPLEX AT 2.1-ANGSTROMS RESOLUTION
1POP 1993-10-31 2.1 X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX
1BP4 1999-08-12 2.2 USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES.
1STF 1994-01-31 2.37 THE REFINED 2.4 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF RECOMBINANT HUMAN STEFIN B IN COMPLEX WITH THE CYSTEINE PROTEINASE PAPAIN: A NOVEL TYPE OF PROTEINASE INHIBITOR INTERACTION
4QRG 2015-08-05 2.5 Crystal structure of I86L mutant of papain
1BQI 1999-08-16 2.5 USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES.
3LFY 2010-07-21 2.6 CTD of Tarocystatin in complex with papain
3TNX 2012-09-12 2.62 Structure of the precursor of a thermostable variant of papain at 2.6 Angstroem resolution
4PAD 1977-04-12 2.8 Binding of chloromethyl ketone substrate analogues to crystalline papain
5PAD 1977-04-12 2.8 BINDING OF CHLOROMETHYL KETONE SUBSTRATE ANALOGUES TO CRYSTALLINE PAPAIN
2PAD 1977-04-12 2.8 BINDING OF CHLOROMETHYL KETONE SUBSTRATE ANALOGUES TO CRYSTALLINE PAPAIN
6PAD 1977-04-12 2.8 Binding of chloromethyl ketone substrate analogues to crystalline papain
1PAD 1977-04-12 2.8 Binding of chloromethyl ketone substrate analogues to crystalline papain
4QRX 2015-08-05 3.14 Crystal structure of pro-papain mutant at pH 4.0
3USV 2012-11-28 3.8 Structure of the precursor of a thermostable variant of papain at 3.8 A resolution from a crystal soaked at pH 4

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Papain P00784 PAPA1_CARPA
90.7 Chymopapain P32956 CYSP3_VASCU
100.0 Chymopapain P14080 PAPA2_CARPA