Factors effecting the thermostability of cysteine proteinases from Carica papaya.


Abstract

Thermal denaturation of four Carica papaya cysteine proteinases (papain, chymopapain, papaya proteinases 3 and 4) was studied as a function of pH using high-sensitivity differential scanning calorimetry. The ratios of calorimetric enthalpy to Van't Hoff enthalpy suggest that, for all these proteins, denaturation occurs as a non two state process, via an intermediate structure. Differences in the thermal stabilities of the proteinases; chymopapain > papaya proteinase 3 > papain > papaya proteinase 4, were correlated to their amino acid sequence to explain the observations in terms of mobility and specific residue mutation. Three-dimensional structures of papain and papaya proteinase 3 were similarly used to illustrate the influence of atomic mobility on stability. Study holds ProTherm entries: 7577, 7578, 7579, 7580, 7581, 7582, 7583, 7584, 7585, 7586, 7587, 7588, 7589, 7590, 7591, 7592, 7593, 7594, 7595, 7596 Extra Details:

Submission Details

ID: MWVJ5Cea3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:34 p.m.

Version: 1

Publication Details
Sumner IG;Harris GW;Taylor MA;Pickersgill RW;Owen AJ;Goodenough PW,Eur. J. Biochem. (1993) Factors effecting the thermostability of cysteine proteinases from Carica papaya. PMID:8508784
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1YAL 1996-06-20T00:00:00+0000 1.7 CARICA PAPAYA CHYMOPAPAIN AT 1.7 ANGSTROMS RESOLUTION
2PAD 1976-11-01T00:00:00+0000 2.8 BINDING OF CHLOROMETHYL KETONE SUBSTRATE ANALOGUES TO CRYSTALLINE PAPAIN
4QRV 2014-07-02T00:00:00+0000 1.98 Crystal structure of I86F mutant of papain
1KHQ 2001-11-30T00:00:00+0000 1.6 ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX
1KHP 2001-11-30T00:00:00+0000 2.0 Monoclinic form of papain/ZLFG-DAM covalent complex
4QRX 2014-07-02T00:00:00+0000 3.14 Crystal structure of pro-papain mutant at pH 4.0
1PPD 1984-11-06T00:00:00+0000 2.0 RESTRAINED LEAST-SQUARES REFINEMENT OF THE SULFHYDRYL PROTEASE PAPAIN TO 2.0 ANGSTROMS
1PPN 1991-10-25T00:00:00+0000 1.6 STRUCTURE OF MONOCLINIC PAPAIN AT 1.60 ANGSTROMS RESOLUTION
4PAD 1976-11-01T00:00:00+0000 2.8 Binding of chloromethyl ketone substrate analogues to crystalline papain
1PPP 1993-03-17T00:00:00+0000 1.9 CRYSTAL STRUCTURE OF PAPAIN-E64-C COMPLEX. BINDING DIVERSITY OF E64-C TO PAPAIN S2 AND S3 SUBSITES

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Papain P00784 PAPA1_CARPA
90.7 Chymopapain P32956 CYSP3_VASCU
100.0 Chymopapain P14080 PAPA2_CARPA