Factors effecting the thermostability of cysteine proteinases from Carica papaya.
Abstract
Thermal denaturation of four Carica papaya cysteine proteinases (papain, chymopapain, papaya proteinases 3 and 4) was studied as a function of pH using high-sensitivity differential scanning calorimetry. The ratios of calorimetric enthalpy to Van't Hoff enthalpy suggest that, for all these proteins, denaturation occurs as a non two state process, via an intermediate structure. Differences in the thermal stabilities of the proteinases; chymopapain > papaya proteinase 3 > papain > papaya proteinase 4, were correlated to their amino acid sequence to explain the observations in terms of mobility and specific residue mutation. Three-dimensional structures of papain and papaya proteinase 3 were similarly used to illustrate the influence of atomic mobility on stability. Study holds ProTherm entries: 7577, 7578, 7579, 7580, 7581, 7582, 7583, 7584, 7585, 7586, 7587, 7588, 7589, 7590, 7591, 7592, 7593, 7594, 7595, 7596 Extra Details:
Submission Details
ID: MWVJ5Cea3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:34 p.m.
Version: 1
Publication Details
Sumner IG;Harris GW;Taylor MA;Pickersgill RW;Owen AJ;Goodenough PW,Eur. J. Biochem. (1993) Factors effecting the thermostability of cysteine proteinases from Carica papaya. PMID:8508784Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 2CIO | 2006-05-18 | 1.5 | The high resolution x-ray structure of papain complexed with fragments of the Trypanosoma brucei cysteine protease inhibitor ICP. |
| 1KHQ | 2003-09-09 | 1.6 | ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX |
| 1PPN | 1994-01-31 | 1.6 | STRUCTURE OF MONOCLINIC PAPAIN AT 1.60 ANGSTROMS RESOLUTION |
| 9PAP | 1986-10-24 | 1.65 | STRUCTURE OF PAPAIN REFINED AT 1.65 ANGSTROMS RESOLUTION |
| 6TCX | 2019-12-04 | 1.65 | Papain bound to a natural cysteine protease inhibitor from Streptomyces mobaraensis |
| 1PIP | 1993-10-31 | 1.7 | CRYSTAL STRUCTURE OF PAPAIN-SUCCINYL-GLN-VAL-VAL-ALA-ALA-P-NITROANILIDE COMPLEX AT 1.7 ANGSTROMS RESOLUTION: NONCOVALENT BINDING MODE OF A COMMON SEQUENCE OF ENDOGENOUS THIOL PROTEASE INHIBITORS |
| 1CVZ | 2000-08-30 | 1.7 | CRYSTAL STRUCTURE ANALYSIS OF PAPAIN WITH CLIK148(CATHEPSIN L SPECIFIC INHIBITOR) |
| 1YAL | 1996-12-23 | 1.7 | CARICA PAPAYA CHYMOPAPAIN AT 1.7 ANGSTROMS RESOLUTION |
| 3E1Z | 2009-01-27 | 1.86 | Crystal structure of the parasite protesase inhibitor chagasin in complex with papain |
| 1PPP | 1994-01-31 | 1.9 | CRYSTAL STRUCTURE OF PAPAIN-E64-C COMPLEX. BINDING DIVERSITY OF E64-C TO PAPAIN S2 AND S3 SUBSITES |
| 4QRV | 2015-08-12 | 1.98 | Crystal structure of I86F mutant of papain |
| 1KHP | 2003-09-09 | 2.0 | Monoclinic form of papain/ZLFG-DAM covalent complex |
| 1PPD | 1985-01-02 | 2.0 | RESTRAINED LEAST-SQUARES REFINEMENT OF THE SULFHYDRYL PROTEASE PAPAIN TO 2.0 ANGSTROMS |
| 3IMA | 2010-02-16 | 2.03 | Complex structure of tarocystatin and papain |
| 1PE6 | 1993-04-15 | 2.1 | REFINED X-RAY STRUCTURE OF PAPAIN(DOT)E-64-C COMPLEX AT 2.1-ANGSTROMS RESOLUTION |
| 6H8T | 2018-09-12 | 2.1 | Crystal structure of Papain modify by achiral Ru(II)complex |
| 1POP | 1993-10-31 | 2.1 | X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX |
| 1BP4 | 1999-08-12 | 2.2 | USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES. |
| 1STF | 1994-01-31 | 2.37 | THE REFINED 2.4 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF RECOMBINANT HUMAN STEFIN B IN COMPLEX WITH THE CYSTEINE PROTEINASE PAPAIN: A NOVEL TYPE OF PROTEINASE INHIBITOR INTERACTION |
| 4QRG | 2015-08-05 | 2.5 | Crystal structure of I86L mutant of papain |
| 1BQI | 1999-08-16 | 2.5 | USE OF PAPAIN AS A MODEL FOR THE STRUCTURE-BASED DESIGN OF CATHEPSIN K INHIBITORS. CRYSTAL STRUCTURES OF TWO PAPAIN INHIBITOR COMPLEXES DEMONSTRATE BINDING TO S'-SUBSITES. |
| 3LFY | 2010-07-21 | 2.6 | CTD of Tarocystatin in complex with papain |
| 3TNX | 2012-09-12 | 2.62 | Structure of the precursor of a thermostable variant of papain at 2.6 Angstroem resolution |
| 6PAD | 1977-04-12 | 2.8 | Binding of chloromethyl ketone substrate analogues to crystalline papain |
| 1PAD | 1977-04-12 | 2.8 | Binding of chloromethyl ketone substrate analogues to crystalline papain |
| 4PAD | 1977-04-12 | 2.8 | Binding of chloromethyl ketone substrate analogues to crystalline papain |
| 2PAD | 1977-04-12 | 2.8 | BINDING OF CHLOROMETHYL KETONE SUBSTRATE ANALOGUES TO CRYSTALLINE PAPAIN |
| 5PAD | 1977-04-12 | 2.8 | BINDING OF CHLOROMETHYL KETONE SUBSTRATE ANALOGUES TO CRYSTALLINE PAPAIN |
| 4QRX | 2015-08-05 | 3.14 | Crystal structure of pro-papain mutant at pH 4.0 |
| 3USV | 2012-11-28 | 3.8 | Structure of the precursor of a thermostable variant of papain at 3.8 A resolution from a crystal soaked at pH 4 |