Adaptation proceeds through the selection of mutations. The distribution of mutant fitness effect and the forces shaping this distribution are therefore keys to predict the evolutionary fate of organisms and their constituents such as enzymes. Here, by producing and sequencing a comprehensive collection of 10,000 mutants, we explore the mutational landscape of one enzyme involved in the spread of antibiotic resistance, the beta-lactamase TEM-1. We measured mutation impact on the enzyme activity through the estimation of amoxicillin minimum inhibitory concentration on a subset of 990 mutants carrying a unique missense mutation, representing 64% of possible amino acid changes in that protein reachable by point mutation. We established that mutation type, solvent accessibility of residues, and the predicted effect of mutations on protein stability primarily determined alone or in combination changes in minimum inhibitory concentration of mutants. Moreover, we were able to capture the drastic modification of the mutational landscape induced by a single stabilizing point mutation (M182T) by a simple model of protein stability. This work thereby provides an integrated framework to study mutation effects and a tool to understand/define better the epistatic interactions.
Submitter: Connie Wang
Submission Date: July 31, 2017, 11:46 a.m.
|Structure ID||Release Date||Resolution||Structure Title|
|1AXB||1997-10-14T00:00:00+0000||2.0||TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI INHIBITED WITH AN ACYLATION TRANSITION STATE ANALOG|
|1BT5||1998-09-02T00:00:00+0000||1.8||CRYSTAL STRUCTURE OF THE IMIPENEM INHIBITED TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI|
|1BTL||1993-11-01T00:00:00+0000||1.8||CRYSTAL STRUCTURE OF ESCHERICHIA COLI TEM1 BETA-LACTAMASE AT 1.8 ANGSTROMS RESOLUTION|
|1CK3||1999-04-27T00:00:00+0000||2.28||N276D MUTANT OF ESCHERICHIA COLI TEM-1 BETA-LACTAMASE|
|1ERM||2000-04-06T00:00:00+0000||1.7||X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXYPHENYL)ETHANE BORONIC ACID|
|1ERO||2000-04-06T00:00:00+0000||2.1||X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID|
|1ERQ||2000-04-06T00:00:00+0000||1.9||X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID|
|1ESU||2000-04-11T00:00:00+0000||2.0||S235A MUTANT OF TEM1 BETA-LACTAMASE|
|1FQG||2000-09-05T00:00:00+0000||1.7||MOLECULAR STRUCTURE OF THE ACYL-ENZYME INTERMEDIATE IN TEM-1 BETA-LACTAMASE|
|1JTD||2001-08-20T00:00:00+0000||2.3||Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase|