Influence of N-cap mutations on the structure and stability of Escherichia coli HPr.


Abstract

This paper describes the effect of N-capping substitutions on the structure and stability of histidine-containing protein (HPr). We have used NMR spectroscopy and conformational stability studies to quantify changes in local and global free energy due to mutagenesis at Ser46, the N-cap for helix B in HPr. Previous NMR studies suggested that helix B of Escherichia coli HPr is dynamic as judged by the rate of exchange of amide protons with solvent. Ser46 was chosen because it is the site of regulatory phosphorylation in HPrs from Gram-positive bacteria, and mutation of this residue to an aspartic acid (S46D) in E. coli HPr (Gram-negative) also makes it a poor substrate in the bacterial phosphoenolpyruvate: sugar phosphotransferase system. Therefore, to understand the mechanism of inactivation of E. coli S46D HPr, as well as the effect of mutagenesis on protein stability, we have characterized three mutants of E. coli HPr: Ser46 has been mutated to an Asp, Asn, and Ala in S46D, S46N, and S46A HPrs, respectively. The results indicate that these N-cap replacements have a marked influence on helix B stability. The effect of mutagenesis on local stability is correlated to global unfolding of HPr. The ability of amino acids to stabilize helix B is Asp > Asn > Ser > Ala. In addition, since there are neither large-scale conformational changes nor detectable changes in the active site of S46D HPr, it is proposed that the loss of phosphotransfer activity of S46D HPr is due to unfavorable steric and/or electrostatic interactions of the Asp with enzyme I of the PTS. Study holds ProTherm entries: 2409, 2410, 2411, 2412, 2413, 2414, 2415, 2416, 14019, 14020, 14021 Extra Details: Escherichia coli HPr; conformational stability; free energy;,electrostatic interactions; helix; histidine

Submission Details

ID: Ksnhsm974

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:18 p.m.

Version: 1

Publication Details
Thapar R;Nicholson EM;Rajagopal P;Waygood EB;Scholtz JM;Klevit RE,Biochemistry (1996) Influence of N-cap mutations on the structure and stability of Escherichia coli HPr. PMID:8784180
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1JEM 1997-07-23 NMR STRUCTURE OF HISTIDINE PHOSPHORYLATED FORM OF THE PHOSPHOCARRIER HISTIDINE CONTAINING PROTEIN FROM BACILLUS SUBTILIS, NMR, 25 STRUCTURES
1VRC 2005-04-19 Complex of enzyme IIAmannose and the histidine-containing phosphocarrier protein HPr from escherichia coli nmr, restrained regularized mean structure
2LRL 2012-05-16 Solution Structures of the IIA(Chitobiose)-HPr complex of the N,N'-Diacetylchitobiose Branch of the Escherichia coli Phosphotransferase System
1HDN 1994-06-22 THE HIGH-RESOLUTION STRUCTURE OF THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI DETERMINED BY RESTRAINED MOLECULAR DYNAMICS FROM NMR NUCLEAR OVERHAUSER EFFECT DATA
3EZE 1998-12-16 COMPLEX OF THE AMINO TERMINAL DOMAIN OF ENZYME I AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE
3EZA 1999-05-25 COMPLEX OF THE AMINO TERMINAL DOMAIN OF ENZYME I AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE
2LRK 2012-05-16 Solution Structures of the IIA(Chitobiose)-HPr complex of the N,N'-Diacetylchitobiose
2XDF 2010-09-22 Solution Structure of the Enzyme I Dimer Complexed with HPr Using Residual Dipolar Couplings and Small Angle X-Ray Scattering
1J6T 2002-11-13 COMPLEX OF ENZYME IIAMTL AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE
2HID 1996-11-08 REFINED NMR STRUCTURE OF PHOSPHOCARRIER HISTIDINE CONTAINING PROTEIN FROM BACILLUS SUBTILIS
1PFH 1995-11-14 THE PHOSPHORYLATED FORM OF THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR
1GGR 2000-11-15 COMPLEX OF ENZYME IIAGLC AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE
3EZB 1999-12-16 COMPLEX OF THE AMINO TERMINAL DOMAIN OF ENZYME I AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI
3CCD 2008-10-21 1.0 1.0 A Structure of Post-Succinimide His15Asp HPr
1OPD 1997-08-20 1.5 HISTIDINE-CONTAINING PROTEIN (HPR), MUTANT WITH SER 46 REPLACED BY ASP (S46D)
1CM3 2000-05-17 1.6 HIS15ASP HPR FROM E. COLI
1CM2 2000-05-17 1.8 STRUCTURE OF HIS15ASP HPR AFTER HYDROLYSIS OF RINGED SPECIES.
1POH 1994-01-31 2.0 THE 2.0 ANGSTROMS RESOLUTION STRUCTURE OF ESCHERICHIA COLI HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR: A REDETERMINATION
1SPH 1995-02-07 2.0 REFINED STRUCTURES OF THE ACTIVE S83C AND IMPAIRED S46D HPRS: EVIDENCE THAT PHOSPHORYLATION DOES NOT REQUIRE A BACKBONE CONFORMATIONAL TRANSITION
2HPR 1993-01-15 2.0 HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR MUTANT WITH MET 51 REPLACED BY VAL AND SER 83 REPLACED BY CYS (M51V, S83C)
4XWJ 2015-10-21 2.1 Histidine-containing phosphocarrier protein (HPr) and antisigma factor Rsd complex
2FEP 2006-06-27 2.45 Structure of truncated CcpA in complex with P-Ser-HPr and Sulfate ions
2JEL 1998-05-27 2.5 JEL42 FAB/HPR COMPLEX
5YA1 2018-07-11 2.7 crystal structure of LsrK-HPr complex with ATP
5YA2 2018-07-11 2.7 Crystal structure of LsrK-HPr complex with ADP
1KKM 2002-08-28 2.8 L.casei HprK/P in complex with B.subtilis P-Ser-HPr
1KKL 2002-08-28 2.8 L.casei HprK/P in complex with B.subtilis HPr
3OQM 2010-12-08 2.96 structure of ccpa-hpr-ser46p-ackA2 complex
3OQO 2011-10-26 2.97 Ccpa-hpr-ser46p-syn cre
5YA0 2018-07-11 3.0 Crystal structure of LsrK and HPr complex
3OQN 2010-12-08 3.3 Structure of ccpa-hpr-ser46-p-gntr-down cre

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
98.8 Phosphocarrier protein HPr P16481 PTHP_KLEPN
100.0 Phosphocarrier protein HPr P0AA09 PTHP_SHIFL
100.0 Phosphocarrier protein HPr P0AA07 PTHP_SALTY
100.0 Phosphocarrier protein HPr P0AA08 PTHP_SALTI
100.0 Phosphocarrier protein HPr P0AA04 PTHP_ECOLI
100.0 Phosphocarrier protein HPr P0AA05 PTHP_ECOL6
100.0 Phosphocarrier protein HPr P0AA06 PTHP_ECO57
98.9 Phosphocarrier protein HPr P08877 PTHP_BACSU