Partially structured self-associating states of acidic fibroblast growth factor.


A combination of near- and far-UV circular dichroism, Fourier-transform infrared spectroscopy, tryptophan fluorescence, size-exclusion chromatography, and a fluorescent extrinsic hydrophobic probe has been employed to characterize partially structured states of human recombinant acidic fibroblast growth factor (aFGF). At low pH, the addition of specific polyanionic ligands or moderate amounts of salts induces states with high secondary but low tertiary structure content. At neutral pH, intermediate amounts of chaotropic agents impose similar partially structured conformational states which also display noncooperative unfolding transitions. Kinetic evidence indicates that similar forms of the protein exist in the first few hundred milliseconds in the refolding pathway of aFGF. The kinetics of their formation appear to be temperature-independent, implying lack of an energy barrier, which is characteristic for further slow folding into the native state. Unlike the native and fully unfolded states, these partially structured conformations exhibit very low solubility, resulting in irreversible aggregation. Potential physiological implications of the existence of such "molten globule" states with regard to the growth factor's transport and biological activity are considered. Study holds ProTherm entries: 4561 Extra Details: additive : EDTA(0.1 mM), tertiary structure; conformational states; noncooperative;,solubility; molten globule

Submission Details

ID: Kshaji3P

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:26 p.m.

Version: 1

Publication Details
Mach H;Ryan JA;Burke CJ;Volkin DB;Middaugh CR,Biochemistry (1993) Partially structured self-associating states of acidic fibroblast growth factor. PMID:7688566
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Fibroblast growth factor 2 P09038 FGF2_HUMAN
100.0 Fibroblast growth factor 2 Q5IS69 FGF2_PANTR
98.7 Fibroblast growth factor 2 P03969 FGF2_BOVIN
98.1 Fibroblast growth factor 2 P20003 FGF2_SHEEP
97.3 Fibroblast growth factor 2 P13109 FGF2_RAT
96.6 Fibroblast growth factor 2 P15655 FGF2_MOUSE
92.9 Fibroblast growth factor 2 P48798 FGF2_MONDO
92.2 Fibroblast growth factor 2 P48800 FGF2_CHICK
99.3 Fibroblast growth factor 2 P48799 FGF2_RABIT
91.1 Fibroblast growth factor 2 Q60487 FGF2_CAVPO