Unfolding of the molten globule state of alpha-lactalbumin studied by 1H NMR.


The urea-induced unfolding of the molten globule state of bovine alpha-lactalbumin was investigated by 1H nuclear magnetic resonance. In the molten globule state, most of the aromatic resonances deviate from their random coil values, indicating that aromatic side chains form some ordered structures in the molten globule state. When the urea concentration increases, the resonances are shifted, and the deviations from the random coil values are diminished. Because the chemical shifts of several random coil peptides are found to be independent of urea concentration, the urea-induced shifts of the resonances in the molten globule state reflect the unfolding transition of some ordered structures. The unfolding transitions measured by individual aromatic resonances do not coincide with each other. The unfolding transition curves obtained from some aromatic resonances are also different from those of the secondary structures measured by circular dichroism spectra. These results clearly show that the unfolding of the molten globule state of alpha-lactalbumin is not a cooperative two-state process. Study holds ProTherm entries: 4638 Extra Details: molten globule state; aromatic resonances; random coil;,ordered structures

Submission Details

ID: KdrP7bG24

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:26 p.m.

Version: 1

Publication Details
Shimizu A;Ikeguchi M;Sugai S,Biochemistry (1993) Unfolding of the molten globule state of alpha-lactalbumin studied by 1H NMR. PMID:8241174
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Alpha-lactalbumin P00711 LALBA_BOVIN
99.3 Alpha-lactalbumin Q9TSR4 LALBA_BOSMU
98.6 Alpha-lactalbumin Q9TSN6 LALBA_BUBBU
97.2 Alpha-lactalbumin P09462 LALBA_SHEEP
95.1 Alpha-lactalbumin P00712 LALBA_CAPHI