Folding kinetics of phage T4 thioredoxin.


Abstract

The folding mechanism for bacteriophage T4 thioredoxin is best described by a four-state box mechanism, N----Uc----Ut----It----N, where N indicates native, Uc the unfolded form with the cis proline isomer, Ut unfolded with the trans proline isomer, and It a compact form with a trans proline isomer. Both manual mixing fluorescence and size-exclusion chromatography indicate that there is a cis-trans proline isomerization that is important to the folding pathway. Furthermore, the data suggest that the cis-trans isomerization can also occur in a compact nativelike state which is referred to as It. The slow phase seen in fluorescence seems to be monitoring the cis-trans isomerization in the compact form, not the isomerization which occurs in the denatured state. Study holds ProTherm entries: 3667, 3668, 3669 Extra Details: measurements were made at far UV four-state box mechanism; fluorescence;,size-exclusion chromatography; cis-trans isomerization

Submission Details

ID: GFdkYHxG4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:22 p.m.

Version: 1

Publication Details
Borden KL;Richards FM,Biochemistry (1990) Folding kinetics of phage T4 thioredoxin. PMID:2186806
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1DE2 1999-11-24 NMR STRUCTURES OF REDUCED BACTERIOPHAGE T4 GLUTAREDOXIN
1DE1 1999-11-24 NMR STRUCTURES OF OXIDIZED BACTERIOPHAGE T4 GLUTAREDOXIN
1ABA 1993-10-31 1.45 THE STRUCTURE OF OXIDIZED BACTERIOPHAGE T4 GLUTAREDOXIN (THIOREDOXIN). REFINEMENT OF NATIVE AND MUTANT PROTEINS
1AAZ 1993-10-31 2.0 THE STRUCTURE OF OXIDIZED BACTERIOPHAGE T4 GLUTAREDOXIN (THIOREDOXIN)

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Glutaredoxin P00276 GLRX_BPT4