Probing the determinants of protein stability: comparison of class A beta-lactamases.


Abstract

Five class A beta-lactamases produced by various mesophilic bacterial species have been compared. Although closely related in primary and overall structures, these enzymes exhibit very different stabilities. In order to investigate the factors responsible for these differences, several features deduced from the amino acid composition and three-dimensional structures were studied for the five proteins. This analysis revealed that higher stability appeared to correlate with increased numbers of intramolecular hydrogen bonds and of salt bridges. By contrast, the global hydrophobicity of the protein seemed to play a relatively minor role. A strongly unfavourable balance between charged residues and the presence of a cis-peptide bond preceding a non-proline residue might also contribute to the particularly low stability of two of the enzymes. Study holds ProTherm entries: 7715, 7716, 7717 Extra Details: cis-peptide bond

Submission Details

ID: FyEc6Fkc

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:34 p.m.

Version: 1

Publication Details
Vanhove M;Houba S;b1motte-Brasseur J;Frère JM,Biochem. J. (1995) Probing the determinants of protein stability: comparison of class A beta-lactamases. PMID:8948443
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
5GHX 2017-01-25 1.24 Crystal structure of beta-lactamase PenP mutant-E166H
4M3K 2014-08-06 1.48 Structure of a single domain camelid antibody fragment cAb-H7S in complex with the BlaP beta-lactamase from Bacillus licheniformis
5ZFL 2019-03-20 1.5 Crystal structure of beta-lactamase PenP mutant E166Y
2WK0 2009-12-01 1.65 Crystal structure of the class A beta-lactamase BS3 inhibited by 6- beta-iodopenicillanate.
1I2W 2002-03-13 1.7 BETA-LACTAMASE FROM BACILLUS LICHENIFORMIS BS3 COMPLEXED WITH CEFOXITIN
1I2S 2002-03-13 1.7 BETA-LACTAMASE FROM BACILLUS LICHENIFORMIS BS3
3SOI 2011-12-14 1.73 Crystallographic structure of Bacillus licheniformis beta-lactamase W210F/W229F/W251F at 1.73 angstrom resolution
1W7F 2006-05-24 1.8 Crystal structure of the class A beta-lactamase BS3 inhibited with isocitrate
3M2J 2011-03-16 1.8 Crystal Structure of fluorescein-labeled Class A -lactamase PenP
3LY3 2011-03-02 1.8 Crystal Structure of fluorophore-labeled Class A Beta-lactamase PenP
3LY4 2011-03-02 1.8 Crystal Structure of fluorophore-labeled Class A -lactamase PenP-E166Cb in complex with penicillin G
3SH9 2011-07-27 1.9 Crystal structure of fluorophore-labeled beta-lactamase PenP in complex with cefotaxime
4N92 2014-09-10 1.93 Crystal structure of beta-lactamse PenP_E166S
5GHZ 2017-01-25 1.93 Crystal structure of beta-lactamase PenP mutant-E166H in complex with cephaloridine as 'pre-deacylation' intermediate
5ZFT 2019-03-20 1.93 Crystal structure of beta-lactamase PenP mutant-E166Y in complex with cephaloridine as 'pre-deacylation' intermediate
4N9K 2014-09-10 1.93 crystal structure of beta-lactamse PenP_E166S in complex with cephaloridine
3SH8 2011-07-27 2.0 Crystal structure of fluorophore-labeled beta-lactamase PenP in complex with cephaloridine
4BLM 1993-01-15 2.0 BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C. REFINEMENT AT 2 ANGSTROMS RESOLUTION AND ANALYSIS OF HYDRATION
2Y91 2012-02-22 2.0 Crystal structure of class A beta-lactamase from Bacillus licheniformis BS3 with clavulanic acid
5ZG6 2019-03-20 2.0 Crystal structure of beta-lactamase PenP mutant-E166Y in complex with cephaloridine as 'post-acylation' intermediate
2BLM 1990-10-15 2.0 BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C AT 2 ANGSTROMS RESOLUTION
1MBL 1994-01-31 2.0 A catalytically-impaired class A beta-lactamase: 2 Angstroms crystal structure and kinetics of the Bacillus licheniformis E166A mutant
5GHY 2017-01-25 2.1 Crystal structure of beta-lactamase PenP mutant-E166H in complex with cephaloridine as 'post-acylation' intermediate
4N9L 2014-09-10 2.3 crystal structure of beta-lactamse PenP_E166S in complex with meropenem
3B3X 2007-11-13 2.5 Crystal structure of class A beta-lactamase of Bacillus licheniformis BS3 with aminocitrate
3SH7 2011-07-27 2.5 Crystal structure of fluorophore-labeled beta-lactamase PenP
5LWF 2017-11-15 2.56 Structure of a single domain camelid antibody fragment cAb-G10S in complex with the BlaP beta-lactamase from Bacillus licheniformis
4N1H 2014-10-08 3.0 Structure of a single-domain camelid antibody fragment cAb-F11N in complex with the BlaP beta-lactamase from Bacillus licheniformis
3M2K 2011-03-16 3.5 Crystal Structure of fluorescein-labeled Class A -beta lactamase PenP in complex with cefotaxime

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Beta-lactamase P00808 BLAC_BACLI