Folding and stability of the b subunit of the F(1)F(0) ATP synthase.


Abstract

The F(1)F(0) ATP synthase is a reversible molecular motor that employs a rotary catalytic cycle to couple a chemiosmotic membrane potential to the formation/hydrolysis of ATP. The multisubunit enzyme contains two copies of the b subunit that form a homodimer as part of a narrow, peripheral stalk structure that connects the membrane (F(0)) and soluble (F(1)) sectors. The three-dimensional structure of the b subunit is unknown making the nature of any interactions or conformational changes within the F(1)F(0) complex difficult to interpret. We have used circular dichroism and analytical ultracentrifugation analyses of a series of N- and C-terminal truncated b proteins to investigate its stability and structure. Thermal denaturation of the b constructs exhibited distinct two-state, cooperative unfolding with T(m) values between 30 and 40 degrees C. CD spectra for the region comprising residues 53-122 (b(53-122)) showed theta;(222)/theta;(208) = 0.99, which reduced to 0.92 in the presence of the hydrophobic solvent trifluoroethanol. Thermodynamic parameters for b(53-122) (DeltaG, DeltaH and DeltaC(p)) were similar to those reported for several nonideal, coiled-coil proteins. Together these results are most consistent with a noncanonical and unstable parallel coiled-coil at the interface of the b dimer. Study holds ProTherm entries: 14865, 14866, 14867, 14868, 14869, 14870, 14871, 14872, 14873, 14874 Extra Details: Residue b24-156. The entire soluble region of the b subunit lacking only the leading 23 residues proposed to span the membrane. protein domains; coiled-coil: thermal denaturation; circular dichroism;,ultracentrifugation

Submission Details

ID: FH7ZeeKo

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:45 p.m.

Version: 1

Publication Details
Revington M;Dunn SD;Shaw GS,Protein Sci. (2002) Folding and stability of the b subunit of the F(1)F(0) ATP synthase. PMID:11967379
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1ATY 1994-12-20 DETERMINATION OF LOCAL PROTEIN STRUCTURE BY SPIN LABEL DIFFERENCE 2D NMR: THE REGION NEIGHBORING ASP61 OF SUBUNIT C OF THE F1FO ATP SYNTHASE
1L6T 2002-07-24 STRUCTURE OF ALA24/ASP61 TO ASP24/ASN61 SUBSTITUTED SUBUNIT C OF ESCHERICHIA COLI ATP SYNTHASE
1C99 1999-11-19 ASP61 DEPROTONATED FORM OF SUBUNIT C OF THE F1FO ATP SYNTHASE OF ESCHERICHIA COLI
1C0V 1999-08-18 SUBUNIT C OF THE F1FO ATP SYNTHASE OF ESCHERICHIA COLI; NMR, 10 STRUCTURES
1IJP 2002-03-27 Solution Structure of Ala20Pro/Pro64Ala substituted subunit c of Escherichia coli ATP synthase
1A91 1998-07-01 SUBUNIT C OF THE F1FO ATP SYNTHASE OF ESCHERICHIA COLI; NMR, 10 STRUCTURES
1C17 1999-11-24 A1C12 SUBCOMPLEX OF F1FO ATP SYNTHASE
1QO1 1999-11-04 3.9 Molecular Architecture of the Rotary Motor in ATP Synthase from Yeast Mitochondria
5T4O 2016-12-28 6.9 Autoinhibited E. coli ATP synthase state 1
5T4P 2016-12-28 7.77 Autoinhibited E. coli ATP synthase state 2
4UTQ 2014-07-30 8.0 A structural model of the active ribosome-bound membrane protein insertase YidC
5T4Q 2017-01-04 8.53 Autoinhibited E. coli ATP synthase state 3

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
93.8 M ATP synthase subunit c A6TG42 ATP6_KLEP7
95.5 M ATP synthase subunit c A7MMW0 ATP6_CROS8
93.8 M ATP synthase subunit c B5XZL8 ATP6_KLEP3
96.0 M ATP synthase subunit c A4WGE9 ATP6_ENT38
97.7 M ATP synthase subunit c A9MJR3 ATP6_SALAR
97.2 M ATP synthase subunit c A8ACP4 ATP6_CITK8
97.7 M ATP synthase subunit c Q7CPE3 ATP6_SALTY
97.7 M ATP synthase subunit c Q8XGC2 ATP6_SALTI
97.7 M ATP synthase subunit c B4TN37 ATP6_SALSV
97.7 M ATP synthase subunit c B5BIP2 ATP6_SALPK
97.7 M ATP synthase subunit c C0Q2N8 ATP6_SALPC
97.7 M ATP synthase subunit c A9MXB2 ATP6_SALPB
97.7 M ATP synthase subunit c Q5PKW6 ATP6_SALPA
97.7 M ATP synthase subunit c B4SYD7 ATP6_SALNS
97.7 M ATP synthase subunit c B4TAX8 ATP6_SALHS
97.7 M ATP synthase subunit c B5RFV7 ATP6_SALG2
97.7 M ATP synthase subunit c B5QVD8 ATP6_SALEP
97.7 M ATP synthase subunit c B5FN39 ATP6_SALDC
97.7 M ATP synthase subunit c Q57HX3 ATP6_SALCH
97.7 M ATP synthase subunit c B5EZ02 ATP6_SALA4
100.0 M ATP synthase subunit c Q329S7 ATP6_SHIDS
100.0 M ATP synthase subunit c B7LK83 ATP6_ESCF3
100.0 M ATP synthase subunit c Q1R4J4 ATP6_ECOUT
100.0 M ATP synthase subunit c B1LL65 ATP6_ECOSM
100.0 M ATP synthase subunit c B7NF54 ATP6_ECOLU
100.0 M ATP synthase subunit c Q8FBS8 ATP6_ECOL6
100.0 M ATP synthase subunit c Q0TAX1 ATP6_ECOL5
100.0 M ATP synthase subunit c A1AHR8 ATP6_ECOK1
100.0 M ATP synthase subunit c B7N241 ATP6_ECO81
100.0 M ATP synthase subunit c B7NR40 ATP6_ECO7I
100.0 M ATP synthase subunit c B7MGF8 ATP6_ECO45
100.0 M ATP synthase subunit c B7UMK3 ATP6_ECO27
99.6 ATP synthase subunit a Q83PJ8 ATP6_SHIFL
99.6 ATP synthase subunit a Q0SYT8 ATP6_SHIF8
100.0 ATP synthase subunit a Q3YVP2 ATP6_SHISS
100.0 ATP synthase subunit a B2TUN7 ATP6_SHIB3
100.0 ATP synthase subunit a B6I3X5 ATP6_ECOSE
100.0 ATP synthase subunit a P0AB98 ATP6_ECOLI
100.0 ATP synthase subunit a B1IX00 ATP6_ECOLC
100.0 ATP synthase subunit a A8A6K1 ATP6_ECOHS
100.0 ATP synthase subunit a B1X9W6 ATP6_ECODH
100.0 ATP synthase subunit a C4ZZ16 ATP6_ECOBW
100.0 ATP synthase subunit a B7M594 ATP6_ECO8A
100.0 ATP synthase subunit a B5YXE2 ATP6_ECO5E
100.0 ATP synthase subunit a P0AB99 ATP6_ECO57
100.0 ATP synthase subunit a B7L888 ATP6_ECO55
100.0 ATP synthase subunit a A7ZTV0 ATP6_ECO24
1138.8 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A0KQY3 ATPL_AERHH
1154.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c C6DJG7 ATPL_PECCP
1154.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q6CYJ0 ATPL_PECAS
1170.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B4F0E2 ATPL_PROMH
1184.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A8G7M3 ATPL_SERP5
1184.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c C5BF35 ATPL_EDWI9
1184.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A1JTD2 ATPL_YERE8
1184.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B2VCA9 ATPL_ERWT9
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B1JR36 ATPL_YERPY
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q663Q3 ATPL_YERPS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A4TSI8 ATPL_YERPP
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q1CCH0 ATPL_YERPN
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A9R5U4 ATPL_YERPG
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68706 ATPL_YERPE
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B2K842 ATPL_YERPB
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q1C090 ATPL_YERPA
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A7FPE5 ATPL_YERP3
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q2NQ91 ATPL_SODGM
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q3YVP1 ATPL_SHISS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68705 ATPL_SHIFL
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q0SYT9 ATPL_SHIF8
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q329S6 ATPL_SHIDS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q31UN7 ATPL_SHIBS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B2TUN8 ATPL_SHIB3
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68704 ATPL_SALTY
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68703 ATPL_SALTI
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B4TN36 ATPL_SALSV
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5BIP1 ATPL_SALPK
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c C0Q2N7 ATPL_SALPC
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A9MXB1 ATPL_SALPB
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q5PKW7 ATPL_SALPA
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B4SYD6 ATPL_SALNS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B4TAX7 ATPL_SALHS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5RFV8 ATPL_SALG2
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5QVD7 ATPL_SALEP
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5FN38 ATPL_SALDC
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q57HX4 ATPL_SALCH
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A9MJR4 ATPL_SALAR
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5EZ01 ATPL_SALA4
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68702 ATPL_PHOLL
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A6TG41 ATPL_KLEP7
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5XZL9 ATPL_KLEP3
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7LK82 ATPL_ESCF3
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A4WGF0 ATPL_ENT38
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q1R4J5 ATPL_ECOUT
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B1LL64 ATPL_ECOSM
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B6I3X4 ATPL_ECOSE
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7NF53 ATPL_ECOLU
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68699 ATPL_ECOLI
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B1IX01 ATPL_ECOLC
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68700 ATPL_ECOL6
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c Q0TAX2 ATPL_ECOL5
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A8A6K0 ATPL_ECOHS
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B1X9W5 ATPL_ECODH
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c C4ZZ15 ATPL_ECOBW
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7M593 ATPL_ECO8A
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7N2H6 ATPL_ECO81
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7NR39 ATPL_ECO7I
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B5YXE1 ATPL_ECO5E
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c P68701 ATPL_ECO57
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7L883 ATPL_ECO55
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7MGF7 ATPL_ECO45
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c B7UMK2 ATPL_ECO27
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A7ZTU9 ATPL_ECO24
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A7MMV9 ATPL_CROS8
1200.0 A,B,C,D,E,F,G,H,I,J,K,L ATP synthase subunit c A8ACP3 ATPL_CITK8
90.4 M ATP synthase subunit c A1JTD4 ATP6_YERE8
90.4 M ATP synthase subunit c B1JR35 ATP6_YERPY
90.4 M ATP synthase subunit c Q663Q2 ATP6_YERPS
90.4 M ATP synthase subunit c A4TSI7 ATP6_YERPP
90.4 M ATP synthase subunit c Q1CCG9 ATP6_YERPN
90.4 M ATP synthase subunit c Q7CFM3 ATP6_YERPE
90.4 M ATP synthase subunit c B2K841 ATP6_YERPB
90.4 M ATP synthase subunit c Q1C089 ATP6_YERPA
90.4 M ATP synthase subunit c A7FPE6 ATP6_YERP3
91.0 M ATP synthase subunit c A8G7M2 ATP6_SERP5
93.2 M ATP synthase subunit c B2VCB0 ATP6_ERWT9
94.4 M ATP synthase subunit c Q2NQ92 ATP6_SODGM
94.4 M ATP synthase subunit c C5BF34 ATP6_EDWI9