Contribution of intra- and intermolecular hydrogen bonds to the conformational stability of human lysozyme(,).


Abstract

In globular proteins, there are intermolecular hydrogen bonds between protein and water molecules, and between water molecules, which are bound with the proteins, in addition to intramolecular hydrogen bonds. To estimate the contribution of these hydrogen bonds to the conformational stability of a protein, the thermodynamic parameters for denaturation and the crystal structures of five Thr to Val and five Thr to Ala mutant human lysozymes were determined. The denaturation Gibbs energy (DeltaG) of Thr to Val and Thr to Ala mutant proteins was changed from 4.0 to -5.6 kJ/mol and from 1.6 to -6.3 kJ/mol, respectively, compared with that of the wild-type protein. The contribution of hydrogen bonds to the stability (DeltaDeltaG(HB)) of the Thr and other mutant human lysozymes previously reported was extracted from the observed stability changes (DeltaDeltaG) with correction for changes in hydrophobicity and side chain conformational entropy between the wild-type and mutant structures. The estimation of the DeltaDeltaG(HB) values of all mutant proteins after removal of hydrogen bonds, including protein-water hydrogen bonds, indicates a favorable contribution of the intra- and intermolecular hydrogen bonds to the protein stability. The net contribution of an intramolecular hydrogen bond (DeltaG(HB[pp])), an intermolecular one between protein and ordered water molecules (DeltaG(HB[pw])), and an intermolecular one between ordered water molecules (DeltaG(HB[ww])) could be estimated to be 8. 5, 5.2, and 5.0 kJ/mol, respectively, for a 3 A long hydrogen bond. This result shows the different contributions to protein stability of intra- and intermolecular hydrogen bonds. The entropic cost due to the introduction of a water molecule (DeltaG(H)()2(O)) could be also estimated to be about 8 kJ/mol. Study holds ProTherm entries: 5817, 5818, 5819, 5820, 5821, 5822, 5823, 5824, 5825, 5826, 5827, 5828, 5829, 5830, 5831, 5832, 5833, 5834, 5835, 5836, 5837, 5838, 5839, 5840, 5841, 5842, 5843, 5844, 5845, 5846, 5847, 5848, 5849, 5850, 5851, 5852, 5853, 5854, 5855, 5856, 5857, 5858, 5859, 5860, 5861, 5862, 5863 Extra Details:

Submission Details

ID: DV9NqoFN

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:31 p.m.

Version: 1

Publication Details
Takano K;Yamagata Y;Funahashi J;Hioki Y;Kuramitsu S;Yutani K,Biochemistry (1999) Contribution of intra- and intermolecular hydrogen bonds to the conformational stability of human lysozyme(,). PMID:10504240
Additional Information

Number of data points 141
Proteins Lysozyme C ; Lysozyme C
Unique complexes 10
Assays/Quantities/Protocols Experimental Assay: dHcal pH:3.2 ; Experimental Assay: Tm pH:3.2 ; Experimental Assay: dHvH pH:3.2 ; Experimental Assay: dHcal pH:2.51 ; Experimental Assay: Tm pH:2.51 ; Experimental Assay: dHvH pH:2.51 ; Experimental Assay: dHcal pH:2.71 ; Experimental Assay: Tm pH:2.71 ; Experimental Assay: dHvH pH:2.71 ; Experimental Assay: dHcal pH:2.81 ; Experimental Assay: Tm pH:2.81 ; Experimental Assay: dHvH pH:2.81 ; Experimental Assay: dHcal pH:3.19 ; Experimental Assay: Tm pH:3.19 ; Experimental Assay: dHvH pH:3.19 ; Experimental Assay: dHcal pH:2.54 ; Experimental Assay: Tm pH:2.54 ; Experimental Assay: dHvH pH:2.54 ; Experimental Assay: dHcal pH:2.9 ; Experimental Assay: Tm pH:2.9 ; Experimental Assay: dHvH pH:2.9 ; Experimental Assay: dHcal pH:3.08 ; Experimental Assay: Tm pH:3.08 ; Experimental Assay: dHvH pH:3.08 ; Experimental Assay: dHcal pH:2.5 ; Experimental Assay: Tm pH:2.5 ; Experimental Assay: dHvH pH:2.5 ; Experimental Assay: dHcal pH:2.7 ; Experimental Assay: Tm pH:2.7 ; Experimental Assay: dHvH pH:2.7 ; Experimental Assay: dHcal pH:2.85 ; Experimental Assay: Tm pH:2.85 ; Experimental Assay: dHvH pH:2.85 ; Experimental Assay: dHcal pH:3.0 ; Experimental Assay: Tm pH:3.0 ; Experimental Assay: dHvH pH:3.0 ; Experimental Assay: dHcal pH:3.1 ; Experimental Assay: Tm pH:3.1 ; Experimental Assay: dHvH pH:3.1 ; Experimental Assay: dHcal pH:2.58 ; Experimental Assay: Tm pH:2.58 ; Experimental Assay: dHvH pH:2.58 ; Experimental Assay: dHcal pH:2.74 ; Experimental Assay: Tm pH:2.74 ; Experimental Assay: dHvH pH:2.74 ; Experimental Assay: dHcal pH:2.82 ; Experimental Assay: Tm pH:2.82 ; Experimental Assay: dHvH pH:2.82 ; Experimental Assay: dHcal pH:3.02 ; Experimental Assay: Tm pH:3.02 ; Experimental Assay: dHvH pH:3.02 ; Experimental Assay: dHcal pH:3.22 ; Experimental Assay: Tm pH:3.22 ; Experimental Assay: dHvH pH:3.22
Libraries Mutations for sequence KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV
Sequence Assay Result Units