A calorimetric study of thermally induced conformational transitions of ribonuclease A and certain of its derivatives.

Abstract

Study holds ProTherm entries: 7334, 7335, 7336, 7337, 7338, 7339, 7340, 7341, 7342, 7343, 7344, 7345, 7346, 7347, 7348, 7349, 7350, 7351, 7352, 7353 Extra Details: conformational transition; cooperativity; two-state process;,calorimatric measurement

Submission Details

ID: DLsq5XWV4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Tsong TY;Hearn RP;Wrathall DP;Sturtevant JM,Biochemistry (1970) A calorimetric study of thermally induced conformational transitions of ribonuclease A and certain of its derivatives. PMID:5465355
Additional Information

Study Summary

Number of data points 60
Proteins Ribonuclease pancreatic ; Ribonuclease pancreatic
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal ionic:NaCl: 0.2 N, pH:1.05, buffers:HCl: - ; Experimental Assay: Tm ionic:NaCl: 0.2 N, pH:1.05, buffers:HCl: - ; Experimental Assay: dHcal ionic:HCl: , pH:0.36, buffers:HCl: - ; Experimental Assay: Tm ionic:HCl: , pH:0.36, buffers:HCl: - ; Experimental Assay: dCp ionic:NaCl: 0.2 N, buffers:None: -, pH:7.8 ; Experimental Assay: dHcal ionic:NaCl: 0.2 N, buffers:None: -, pH:7.8 ; Experimental Assay: Tm ionic:NaCl: 0.2 N, buffers:None: -, pH:7.8 ; Experimental Assay: dHvH ionic:NaCl: 0.2 N, buffers:None: -, pH:7.8 ; Experimental Assay: dCp ionic:NaCl: 0.2 N, buffers:None: -, pH:7.0 ; Experimental Assay: dHcal ionic:NaCl: 0.2 N, buffers:None: -, pH:7.0 ; Experimental Assay: Tm ionic:NaCl: 0.2 N, buffers:None: -, pH:7.0 ; Experimental Assay: dHvH ionic:NaCl: 0.2 N, buffers:None: -, pH:7.0 ; Experimental Assay: dCp pH:6.23, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: dHcal pH:6.23, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: Tm pH:6.23, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: dHvH pH:6.23, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: dCp pH:5.0, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: dHcal pH:5.0, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: Tm pH:5.0, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: dHvH pH:5.0, ionic:: , buffers:acetate: 0.2 N ; Experimental Assay: dCp ionic:: , buffers:acetate: 0.2 N, pH:4.04 ; Experimental Assay: dHcal ionic:: , buffers:acetate: 0.2 N, pH:4.04 ; Experimental Assay: Tm ionic:: , buffers:acetate: 0.2 N, pH:4.04 ; Experimental Assay: dHvH ionic:: , buffers:acetate: 0.2 N, pH:4.04 ; Experimental Assay: dCp ionic:: , pH:3.28, buffers:glycine: 0.2 N ; Experimental Assay: dHcal ionic:: , buffers:glycine: 0.2 N, pH:3.28 ; Experimental Assay: Tm ionic:: , pH:3.28, buffers:glycine: 0.2 N ; Experimental Assay: dHvH ionic:: , pH:3.28, buffers:glycine: 0.2 N ; Experimental Assay: dCp pH:2.8, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: dHcal pH:2.8, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: Tm pH:2.8, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: dHvH ionic:: , pH:2.8, buffers:glycine: 0.2 N ; Experimental Assay: dCp pH:2.02, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: dHcal pH:2.02, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: Tm pH:2.02, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: dHvH pH:2.02, ionic:: , buffers:glycine: 0.2 N ; Experimental Assay: dCp ionic:NaCl: 0.1 N, pH:1.05, buffers:glycine: 0.2 N ; Experimental Assay: dHcal ionic:NaCl: 0.1 N, pH:1.05, buffers:glycine: 0.2 N ; Experimental Assay: Tm ionic:NaCl: 0.1 N, pH:1.05, buffers:glycine: 0.2 N ; Experimental Assay: dHvH ionic:NaCl: 0.1 N, pH:1.05, buffers:glycine: 0.2 N ; Experimental Assay: dCp ionic:HCl: , buffers:glycine: 0.2 N, pH:0.36 ; Experimental Assay: dHcal ionic:HCl: , buffers:glycine: 0.2 N, pH:0.36 ; Experimental Assay: Tm ionic:HCl: , buffers:glycine: 0.2 N, pH:0.36 ; Experimental Assay: dHvH ionic:HCl: , buffers:glycine: 0.2 N, pH:0.36
Libraries Mutations for sequence KETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Ribonuclease pancreatic P61824 RNAS1_BISBI
100.0 Ribonuclease pancreatic P61823 RNAS1_BOVIN
96.8 Ribonuclease pancreatic P67926 RNAS1_CAPHI
96.8 Ribonuclease pancreatic P67927 RNAS1_SHEEP
95.2 Ribonuclease pancreatic P00657 RNAS1_BUBBU
96.0 Ribonuclease pancreatic P07847 RNAS1_AEPME
93.5 Ribonuclease pancreatic P07848 RNAS1_EUDTH
95.2 Ribonuclease pancreatic P00660 RNAS1_CONTA
92.7 Ribonuclease pancreatic P00668 RNAS1_ANTAM
90.3 Ribonuclease pancreatic P00662 RNAS1_GIRCA
96.0 Ribonuclease pancreatic Q29606 RNAS1_ORYLE