A reversible unfolding reaction of swine pepsin; implications for pepsinogen's folding mechanism.


Abstract

Above pH 6, swine pepsin undergoes a conformational change to a neutral form which has 80% of the secondary structure of the native protein. In contrast to native pepsin, this form of the enzyme can be reversibly unfolded by urea in a rapid, cooperative reaction. Since all of pepsin's sequence is present in its precursor pepsinogen, it is likely that this neutral structure is present in one or more of the transient intermediates previously detected in the reversible unfolding reaction of the zymogen. The mechanism of this rapid reaction may resemble early steps in protein folding. Study holds ProTherm entries: 10023 Extra Details: conformation change; secondary structure; transient intermidiates;,protein folding

Submission Details

ID: CkuQmARz

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:39 p.m.

Version: 1

Publication Details
McPhie P,Biochem. Biophys. Res. Commun. (1989) A reversible unfolding reaction of swine pepsin; implications for pepsinogen's folding mechanism. PMID:2492190
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3PSG 1993-01-15 1.65 THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN
4PEP 1990-04-15 1.8 THE MOLECULAR AND CRYSTAL STRUCTURES OF MONOCLINIC PORCINE PEPSIN REFINED AT 1.8 ANGSTROMS RESOLUTION
2PSG 1992-10-15 1.8 REFINED STRUCTURE OF PORCINE PEPSINOGEN AT 1.8 ANGSTROMS RESOLUTION
3PEP 1990-04-15 2.3 REVISED 2.3 ANGSTROMS STRUCTURE OF PORCINE PEPSIN. EVIDENCE FOR A FLEXIBLE SUBDOMAIN
5PEP 1990-07-15 2.34 X-RAY ANALYSES OF ASPARTIC PROTEASES. II. THREE-DIMENSIONAL STRUCTURE OF THE HEXAGONAL CRYSTAL FORM OF PORCINE PEPSIN AT 2.3 ANGSTROMS RESOLUTION
1F34 2001-02-01 2.45 CRYSTAL STRUCTURE OF ASCARIS PEPSIN INHIBITOR-3 BOUND TO PORCINE PEPSIN
1PSA 1994-01-31 2.9 STRUCTURE OF A PEPSIN(SLASH)RENIN INHIBITOR COMPLEX REVEALS A NOVEL CRYSTAL PACKING INDUCED BY MINOR CHEMICAL ALTERATIONS IN THE INHIBITOR
1YX9 2005-05-24 3.0 Effect of Dimethyl Sulphoxide on the crystal structure of Porcine Pepsin

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Pepsin A P00791 PEPA_PIG