Guanidinium chloride- and urea-induced unfolding of the dimeric enzyme glucose oxidase.


Abstract

We have carried out a systematic study on the guanidinium chloride- and urea-induced unfolding of glucose oxidase from Aspergillus niger, an acidic dimeric enzyme, using various optical spectroscopic techniques, enzymatic activity measurements, glutaraldehyde cross-linking, and differential scanning calorimetry. The urea-induced unfolding of GOD was a two-state process with dissociation and unfolding of the native dimeric enzyme molecule occurring in a single step. On the contrary, the GdmCl-induced unfolding of GOD was a multiphasic process with stabilization of a conformation more compact than the native enzyme at low GdmCl concentrations and dissociation along with unfolding of enzyme at higher concentrations of GdmCl. The GdmCl-stabilized compact dimeric intermediate of GOD showed an enhanced stability against thermal and urea denaturation as compared to the native GOD dimer. Comparative studies on GOD using GdmCl and NaCl demonstrated that binding of the Gdm(+) cation to the enzyme results in stabilization of the compact dimeric intermediate of the enzyme at low GdmCl concentrations. An interesting observation was that a slight difference in the concentration of urea and GdmCl associated with the unfolding of GOD was observed, which is in violation of the 2-fold rule for urea and GdmCl denaturation of proteins. This is the first report where violation of the 2-fold rule has been observed for a multimeric protein. Study holds ProTherm entries: 13010, 13011 Extra Details: optical spectroscopic technique; two-state process; dimeric intermediate;,multiphasic process

Submission Details

ID: CCzJxj6B

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:44 p.m.

Version: 1

Publication Details
Akhtar MS;Ahmad A;Bhakuni V,Biochemistry (2002) Guanidinium chloride- and urea-induced unfolding of the dimeric enzyme glucose oxidase. PMID:11888301
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3QVP 2011-06-01 1.2 Crystal structure of glucose oxidase for space group C2221 at 1.2 A resolution
3QVR 2011-06-01 1.3 Crystal structure of glucose oxidase for space group P3121 at 1.3 A resolution.
5NIW 2017-11-15 1.8 Glucose oxydase mutant A2
5NIT 2017-11-15 1.87 Glucose oxidase mutant A2
1CF3 1999-03-26 1.9 GLUCOSE OXIDASE FROM APERGILLUS NIGER
1GAL 1993-10-31 2.3 CRYSTAL STRUCTURE OF GLUCOSE OXIDASE FROM ASPERGILLUS NIGER: REFINED AT 2.3 ANGSTROMS RESOLUTION

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Glucose oxidase P13006 GOX_ASPNG