Lactoperoxidase consists of domains: a scanning calorimetric study.


Abstract

Thermal unfolding of lactoperoxidase (donor: hydrogen-peroxide oxidoreductase, EC 1.11.1.7) was studied by means of differential scanning calorimetry and optical methods. The protein consists of at least two domains differing in thermostability. The prosthetic group belongs to the domain of lower thermostability. Thermodynamic parameters of protein unfolding are given and found to be similar to corresponding data for globular proteins. Study holds ProTherm entries: 7311, 7312, 7313, 7314, 7315, 7316, 7317, 7318, 7319, 7320 Extra Details: hydrogen-peroxide oxidoreductase; optical method; domain;,prosthetic group

Submission Details

ID: C2ZBaVmA4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Pfeil W;Ohlsson PI,Biochim. Biophys. Acta (1986) Lactoperoxidase consists of domains: a scanning calorimetric study. PMID:3730397
Additional Information

Number of data points 30
Proteins Lactoperoxidase
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal pH:3.0 ; Experimental Assay: Tm pH:3.0 ; Experimental Assay: dHvH pH:3.0 ; Experimental Assay: dHcal pH:3.25 ; Experimental Assay: Tm pH:3.25 ; Experimental Assay: dHvH pH:3.25 ; Experimental Assay: dHcal pH:3.5 ; Experimental Assay: Tm pH:3.5 ; Experimental Assay: dHvH pH:3.5 ; Experimental Assay: dHcal pH:3.75 ; Experimental Assay: Tm pH:3.75 ; Experimental Assay: dHvH pH:3.75 ; Experimental Assay: dHcal pH:4.0 ; Experimental Assay: Tm pH:4.0 ; Experimental Assay: dHvH pH:4.0 ; Experimental Assay: dHcal pH:4.25 ; Experimental Assay: Tm pH:4.25 ; Experimental Assay: dHvH pH:4.25 ; Experimental Assay: dHcal pH:4.5 ; Experimental Assay: Tm pH:4.5 ; Experimental Assay: dHvH pH:4.5 ; Experimental Assay: dHcal pH:5.0 ; Experimental Assay: Tm pH:5.0 ; Experimental Assay: dHvH pH:5.0 ; Experimental Assay: dHcal ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: Tm ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHvH ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHcal pH:6.05 ; Experimental Assay: Tm pH:6.05 ; Experimental Assay: dHvH pH:6.05
Libraries Mutations for sequence MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGRTRTAIRNGQVWEESLKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLVRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKVSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN
Sequence Assay Result Units