Lactoperoxidase consists of domains: a scanning calorimetric study.


Abstract

Thermal unfolding of lactoperoxidase (donor: hydrogen-peroxide oxidoreductase, EC 1.11.1.7) was studied by means of differential scanning calorimetry and optical methods. The protein consists of at least two domains differing in thermostability. The prosthetic group belongs to the domain of lower thermostability. Thermodynamic parameters of protein unfolding are given and found to be similar to corresponding data for globular proteins. Study holds ProTherm entries: 7311, 7312, 7313, 7314, 7315, 7316, 7317, 7318, 7319, 7320 Extra Details: hydrogen-peroxide oxidoreductase; optical method; domain;,prosthetic group

Submission Details

ID: C2ZBaVmA4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Pfeil W;Ohlsson PI,Biochim. Biophys. Acta (1986) Lactoperoxidase consists of domains: a scanning calorimetric study. PMID:3730397
Additional Information

Study Summary

Number of data points 30
Proteins Lactoperoxidase
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal pH:3.0 ; Experimental Assay: Tm pH:3.0 ; Experimental Assay: dHvH pH:3.0 ; Experimental Assay: dHcal pH:3.25 ; Experimental Assay: Tm pH:3.25 ; Experimental Assay: dHvH pH:3.25 ; Experimental Assay: dHcal pH:3.5 ; Experimental Assay: Tm pH:3.5 ; Experimental Assay: dHvH pH:3.5 ; Experimental Assay: dHcal pH:3.75 ; Experimental Assay: Tm pH:3.75 ; Experimental Assay: dHvH pH:3.75 ; Experimental Assay: dHcal pH:4.0 ; Experimental Assay: Tm pH:4.0 ; Experimental Assay: dHvH pH:4.0 ; Experimental Assay: dHcal pH:4.25 ; Experimental Assay: Tm pH:4.25 ; Experimental Assay: dHvH pH:4.25 ; Experimental Assay: dHcal pH:4.5 ; Experimental Assay: Tm pH:4.5 ; Experimental Assay: dHvH pH:4.5 ; Experimental Assay: dHcal pH:5.0 ; Experimental Assay: Tm pH:5.0 ; Experimental Assay: dHvH pH:5.0 ; Experimental Assay: dHcal ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: Tm ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHvH ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHcal pH:6.05 ; Experimental Assay: Tm pH:6.05 ; Experimental Assay: dHvH pH:6.05
Libraries Mutations for sequence MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGRTRTAIRNGQVWEESLKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLVRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKVSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3QL6 2011-03-02 1.7 Crystal structure of the complex of bovine lactoperoxidase with nimesulide at 1.7 A resolution
3Q9K 2011-03-02 1.7 Crystal structure of bovine lactoperoxidase complexed with Phenyl isothiocyanate at 1.7 A resolution
3NYH 2010-10-20 1.77 Crystal structure of lactoperoxidase complexed simultaneously with thiocyanate ion, iodide ion, bromide ion, chloride ion through the substrate diffusion channel reveals a preferential queue of the inorganic substrates towards the distal heme cavity
5ZWW 2018-06-13 1.77 CRYSTAL STRUCTURE OF THE COMPLEX OF LACTOPEROXIDASE WITH HYDROGEN PEROXIDE AT 1.77A RESOLUTION
3OGW 2010-12-01 1.89 Structure of the complex of bovine lactoperoxidase with indomethacin at 1.9A resolution
6A4Y 2018-07-04 1.92 Crystal structure of bovine lactoperoxidase with partial occupancies of iodide and SCN- ions at the substrate binding site on the distal heme side at 1.92 A resolution
5GLS 2016-07-27 1.93 Structure of bovine Lactoperoxidase with a partially modified covalent bond with heme moiety
4KSZ 2013-07-10 1.98 Crystal structure of bovine lactoperoxidase complexed with cystiene at 1.98A resolution
5B72 2016-07-13 1.98 Crystal structure of bovine lactoperoxidase with a broken covalent bond between Glu258 and heme moiety at 1.98 A resolution.
4NT3 2013-12-25 1.99 Structure of the complex of bovine lactoperoxidase with 3,3'-dipyridyl ketone at 1.99 A resolution
3S4F 2011-06-08 2.0 Crystal structure of the complex of bovine lactoperoxidase with 1H-pyrazolo[4,3-c] pyridine at 1.99 A resolution
3V6Q 2012-02-15 2.0 Crystal structure of the complex of bovine lactoperoxidase with Carbon monoxide at 2.0 A resolution
3R4X 2011-08-03 2.01 Crystal structure of bovine lactoperoxidase complexed with pyrazine-2-carboxamide at 2 A resolution
5WV3 2017-02-15 2.07 Crystal structure of bovine lactoperoxidase with a partial Glu258-heme linkage at 2.07 A resolution.
4Y55 2015-03-25 2.1 Crystal structure of Buffalo lactoperoxidase with Rhodanide at 2.09 Angstrom resolution
5ZGS 2018-05-23 2.2 Crystal structure of the complex of bovine lactoperoxidase with multiple SCN and OSCN ions in the distal heme cavity
3KRQ 2010-05-26 2.25 Crystal structure of the complex of lactoperoxidase with a potent inhibitor amino-triazole at 2.2a resolution
5GH0 2016-06-29 2.3 Crystal structure of the complex of bovine lactoperoxidase with mercaptoimidazole at 2.3 A resolution
3BXI 2008-03-25 2.3 Structure of the complex of bovine lactoperoxidase with its catalyzed product hypothiocyanate ion at 2.3A resolution
4NJB 2013-11-27 2.31 Crystal structure of the complex of lactoperoxidase from bovine with 3,3-oxydipyridine at 2.31 A resolution
3GCJ 2009-03-31 2.34 Mode of ligand binding and assignment of subsites in mammalian peroxidases: crystal structure of lactoperoxidase complexes with acetyl salycylic acid, salicylhydroxamic acid and benzylhydroxamic acid
2PT3 2007-05-22 2.34 Crystal structure of bovine lactoperoxidase at 2.34 A resolution reveals multiple anion binding sites
2QPK 2007-08-07 2.34 Crystal structure of the complex of bovine lactoperoxidase with salicylhydroxamic acid at 2.34 A resolution
3TGY 2011-09-21 2.35 Crystal structure of the complex of Bovine Lactoperoxidase with Ascorbic acid at 2.35 A resolution
3ERH 2009-03-31 2.4 First structural evidence of substrate specificity in mammalian peroxidases: Crystal structures of substrate complexes with lactoperoxidases from two different species
4PNX 2014-03-12 2.41 Crystal structure of the complex of lactoperoxidase with bromo methane at 2.41 angstrom resolution
3PY4 2011-01-05 2.42 Crystal structure of bovine lactoperoxidase in complex with paracetamol at 2.4A resolution
4GN6 2012-09-19 2.42 Structure of paracetamol bound bovine lactoperoxidase at 2.45A resolution.
3FNL 2009-01-27 2.48 Crystal Structure of the Complex of Buffalo Lactoperoxidase with Salicylhydroxamic Acid at 2.48 A Resolution
3GC1 2009-03-31 2.5 Crystal structure of bovine lactoperoxidase
3GCL 2009-03-31 2.5 Mode of ligand binding and assignment of subsites in mammalian peroxidases: crystal structure of lactoperoxidase complexes with acetyl salycylic acid, salicylhydroxamic acid and benzylhydroxamic acid
3ERI 2009-03-31 2.5 First structural evidence of substrate specificity in mammalian peroxidases: Crystal structures of substrate complexes with lactoperoxidases from two different species
2QQT 2007-08-07 2.5 Crystal structure of the complex of bovine lactoperoxidase with acetyl salicylic acid at 2.5 A resolution
2QRB 2007-08-14 2.5 Crystal structure of chloride saturated bovine lactoperoxidase at 2.5 A resolution shows multiple halide binding sites
4GM7 2012-09-19 2.6 Structure of cinnamic acid bound bovine lactoperoxidase at 2.6A resolution.
3R5O 2011-08-17 2.6 Crystal structure of the complex of bovine lactoperoxidase with 4-allyl-2-methoxyphenol at 2.6 A resolution
3UBA 2011-12-28 2.65 Crystal structure of the complex of bovine lactoperoxidase with p-hydroxycinnamic acid at 2.6 A resolution
2PUM 2007-05-22 2.7 Crystal structure of bovine lactoperoxidase complex with catechol and iodide at 2.7 A resolution
3FAQ 2009-03-31 2.7 Crystal structure of lactoperoxidase complex with cyanide
3I6N 2009-10-13 2.7 Mode of Binding of the Tuberculosis Prodrug Isoniazid to Peroxidases: Crystal Structure of Bovine Lactoperoxidase with Isoniazid at 2.7 Resolution
2GJM 2006-05-02 2.75 Crystal structure of Buffalo lactoperoxidase at 2.75A resolution
2O86 2006-12-26 2.8 Crystal structure of a ternary complex of buffalo lactoperoxidase with nitrate and iodide at 2.8 A resolution
3GCK 2009-03-31 2.9 Mode of ligand binding and assignment of subsites in mammalian peroxidases: crystal structure of lactoperoxidase complexes with acetyl salycylic acid, salicylhydroxamic acid and benzylhydroxamic acid
2NQX 2006-11-21 2.95 Crystal Structure of bovine lactoperoxidase with iodide ions at 2.9A resolution
2IPS 2006-10-24 3.1 Crystal structure of a ternary complex of bovine lactoperoxidase with thiocyanate and iodide at 3.1 A resolution
2Z5Z 2007-08-14 3.5 Crystal structure of the complex of buffalo Lactoperoxidase with fluoride ion at 3.5A resolution

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.4 Lactoperoxidase A5JUY8 PERL_BUBBU
100.0 Lactoperoxidase P80025 PERL_BOVIN