Lactoperoxidase consists of domains: a scanning calorimetric study.


Abstract

Thermal unfolding of lactoperoxidase (donor: hydrogen-peroxide oxidoreductase, EC 1.11.1.7) was studied by means of differential scanning calorimetry and optical methods. The protein consists of at least two domains differing in thermostability. The prosthetic group belongs to the domain of lower thermostability. Thermodynamic parameters of protein unfolding are given and found to be similar to corresponding data for globular proteins. Study holds ProTherm entries: 7311, 7312, 7313, 7314, 7315, 7316, 7317, 7318, 7319, 7320 Extra Details: hydrogen-peroxide oxidoreductase; optical method; domain;,prosthetic group

Submission Details

ID: C2ZBaVmA4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Pfeil W;Ohlsson PI,Biochim. Biophys. Acta (1986) Lactoperoxidase consists of domains: a scanning calorimetric study. PMID:3730397
Additional Information

Study Summary

Number of data points 30
Proteins Lactoperoxidase
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal pH:3.0 ; Experimental Assay: Tm pH:3.0 ; Experimental Assay: dHvH pH:3.0 ; Experimental Assay: dHcal pH:3.25 ; Experimental Assay: Tm pH:3.25 ; Experimental Assay: dHvH pH:3.25 ; Experimental Assay: dHcal pH:3.5 ; Experimental Assay: Tm pH:3.5 ; Experimental Assay: dHvH pH:3.5 ; Experimental Assay: dHcal pH:3.75 ; Experimental Assay: Tm pH:3.75 ; Experimental Assay: dHvH pH:3.75 ; Experimental Assay: dHcal pH:4.0 ; Experimental Assay: Tm pH:4.0 ; Experimental Assay: dHvH pH:4.0 ; Experimental Assay: dHcal pH:4.25 ; Experimental Assay: Tm pH:4.25 ; Experimental Assay: dHvH pH:4.25 ; Experimental Assay: dHcal pH:4.5 ; Experimental Assay: Tm pH:4.5 ; Experimental Assay: dHvH pH:4.5 ; Experimental Assay: dHcal pH:5.0 ; Experimental Assay: Tm pH:5.0 ; Experimental Assay: dHvH pH:5.0 ; Experimental Assay: dHcal ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: Tm ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHvH ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHcal pH:6.05 ; Experimental Assay: Tm pH:6.05 ; Experimental Assay: dHvH pH:6.05
Libraries Mutations for sequence MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGRTRTAIRNGQVWEESLKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLVRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKVSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
3FAQ 2008-11-18T00:00:00+0000 2.7 Crystal structure of lactoperoxidase complex with cyanide
2Z5Z 2007-07-20T00:00:00+0000 3.5 Crystal structure of the complex of buffalo Lactoperoxidase with fluoride ion at 3.5A resolution
3ERH 2008-10-02T00:00:00+0000 2.4 First structural evidence of substrate specificity in mammalian peroxidases: Crystal structures of substrate complexes with lactoperoxidases from two different species
2GJM 2006-03-31T00:00:00+0000 2.75 Crystal structure of Buffalo lactoperoxidase at 2.75A resolution
2O86 2006-12-12T00:00:00+0000 2.8 Crystal structure of a ternary complex of buffalo lactoperoxidase with nitrate and iodide at 2.8 A resolution
3FNL 2008-12-25T00:00:00+0000 2.48 Crystal Structure of the Complex of Buffalo Lactoperoxidase with Salicylhydroxamic Acid at 2.48 A Resolution
4Y55 2015-02-11T00:00:00+0000 2.1 Crystal structure of Buffalo lactoperoxidase with Rhodanide at 2.09 Angstrom resolution
3S4F 2011-05-19T00:00:00+0000 2.0 Crystal structure of the complex of bovine lactoperoxidase with 1H-pyrazolo[4,3-c] pyridine at 1.99 A resolution
2PT3 2007-05-08T00:00:00+0000 2.34 Crystal structure of bovine lactoperoxidase at 2.34 A resolution reveals multiple anion binding sites
4GN6 2012-08-16T00:00:00+0000 2.42 Structure of paracetamol bound bovine lactoperoxidase at 2.45A resolution.

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.4 Lactoperoxidase A5JUY8 PERL_BUBBU
100.0 Lactoperoxidase P80025 PERL_BOVIN