Lactoperoxidase consists of domains: a scanning calorimetric study.


Abstract

Thermal unfolding of lactoperoxidase (donor: hydrogen-peroxide oxidoreductase, EC 1.11.1.7) was studied by means of differential scanning calorimetry and optical methods. The protein consists of at least two domains differing in thermostability. The prosthetic group belongs to the domain of lower thermostability. Thermodynamic parameters of protein unfolding are given and found to be similar to corresponding data for globular proteins. Study holds ProTherm entries: 7311, 7312, 7313, 7314, 7315, 7316, 7317, 7318, 7319, 7320 Extra Details: hydrogen-peroxide oxidoreductase; optical method; domain;,prosthetic group

Submission Details

ID: C2ZBaVmA4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Pfeil W;Ohlsson PI,Biochim. Biophys. Acta (1986) Lactoperoxidase consists of domains: a scanning calorimetric study. PMID:3730397
Additional Information

Study Summary

Number of data points 30
Proteins Lactoperoxidase
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal pH:3.0 ; Experimental Assay: Tm pH:3.0 ; Experimental Assay: dHvH pH:3.0 ; Experimental Assay: dHcal pH:3.25 ; Experimental Assay: Tm pH:3.25 ; Experimental Assay: dHvH pH:3.25 ; Experimental Assay: dHcal pH:3.5 ; Experimental Assay: Tm pH:3.5 ; Experimental Assay: dHvH pH:3.5 ; Experimental Assay: dHcal pH:3.75 ; Experimental Assay: Tm pH:3.75 ; Experimental Assay: dHvH pH:3.75 ; Experimental Assay: dHcal pH:4.0 ; Experimental Assay: Tm pH:4.0 ; Experimental Assay: dHvH pH:4.0 ; Experimental Assay: dHcal pH:4.25 ; Experimental Assay: Tm pH:4.25 ; Experimental Assay: dHvH pH:4.25 ; Experimental Assay: dHcal pH:4.5 ; Experimental Assay: Tm pH:4.5 ; Experimental Assay: dHvH pH:4.5 ; Experimental Assay: dHcal pH:5.0 ; Experimental Assay: Tm pH:5.0 ; Experimental Assay: dHvH pH:5.0 ; Experimental Assay: dHcal ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: Tm ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHvH ionic:KCl: 0.1 M, pH:5.0 ; Experimental Assay: dHcal pH:6.05 ; Experimental Assay: Tm pH:6.05 ; Experimental Assay: dHvH pH:6.05
Libraries Mutations for sequence MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGRTRTAIRNGQVWEESLKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLVRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKVSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Lactoperoxidase P80025 PERL_BOVIN
99.4 Lactoperoxidase A5JUY8 PERL_BUBBU