Lactoperoxidase consists of domains: a scanning calorimetric study.
Abstract
Thermal unfolding of lactoperoxidase (donor: hydrogen-peroxide oxidoreductase, EC 1.11.1.7) was studied by means of differential scanning calorimetry and optical methods. The protein consists of at least two domains differing in thermostability. The prosthetic group belongs to the domain of lower thermostability. Thermodynamic parameters of protein unfolding are given and found to be similar to corresponding data for globular proteins. Study holds ProTherm entries: 7311, 7312, 7313, 7314, 7315, 7316, 7317, 7318, 7319, 7320 Extra Details: hydrogen-peroxide oxidoreductase; optical method; domain;,prosthetic group
Submission Details
ID: C2ZBaVmA4
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:33 p.m.
Version: 1
Publication Details
Pfeil W;Ohlsson PI,Biochim. Biophys. Acta (1986) Lactoperoxidase consists of domains: a scanning calorimetric study. PMID:3730397Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
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Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 3QL6 | 2011-03-02 | 1.7 | Crystal structure of the complex of bovine lactoperoxidase with nimesulide at 1.7 A resolution |
| 3Q9K | 2011-03-02 | 1.7 | Crystal structure of bovine lactoperoxidase complexed with Phenyl isothiocyanate at 1.7 A resolution |
| 3NYH | 2010-10-20 | 1.77 | Crystal structure of lactoperoxidase complexed simultaneously with thiocyanate ion, iodide ion, bromide ion, chloride ion through the substrate diffusion channel reveals a preferential queue of the inorganic substrates towards the distal heme cavity |
| 5ZWW | 2018-06-13 | 1.77 | CRYSTAL STRUCTURE OF THE COMPLEX OF LACTOPEROXIDASE WITH HYDROGEN PEROXIDE AT 1.77A RESOLUTION |
| 3OGW | 2010-12-01 | 1.89 | Structure of the complex of bovine lactoperoxidase with indomethacin at 1.9A resolution |
| 6A4Y | 2018-07-04 | 1.92 | Crystal structure of bovine lactoperoxidase with partial occupancies of iodide and SCN- ions at the substrate binding site on the distal heme side at 1.92 A resolution |
| 5GLS | 2016-07-27 | 1.93 | Structure of bovine Lactoperoxidase with a partially modified covalent bond with heme moiety |
| 4KSZ | 2013-07-10 | 1.98 | Crystal structure of bovine lactoperoxidase complexed with cystiene at 1.98A resolution |
| 5B72 | 2016-07-13 | 1.98 | Crystal structure of bovine lactoperoxidase with a broken covalent bond between Glu258 and heme moiety at 1.98 A resolution. |
| 4NT3 | 2013-12-25 | 1.99 | Structure of the complex of bovine lactoperoxidase with 3,3'-dipyridyl ketone at 1.99 A resolution |
| 3S4F | 2011-06-08 | 2.0 | Crystal structure of the complex of bovine lactoperoxidase with 1H-pyrazolo[4,3-c] pyridine at 1.99 A resolution |
| 3V6Q | 2012-02-15 | 2.0 | Crystal structure of the complex of bovine lactoperoxidase with Carbon monoxide at 2.0 A resolution |
| 3R4X | 2011-08-03 | 2.01 | Crystal structure of bovine lactoperoxidase complexed with pyrazine-2-carboxamide at 2 A resolution |
| 5WV3 | 2017-02-15 | 2.07 | Crystal structure of bovine lactoperoxidase with a partial Glu258-heme linkage at 2.07 A resolution. |
| 4Y55 | 2015-03-25 | 2.1 | Crystal structure of Buffalo lactoperoxidase with Rhodanide at 2.09 Angstrom resolution |
| 5ZGS | 2018-05-23 | 2.2 | Crystal structure of the complex of bovine lactoperoxidase with multiple SCN and OSCN ions in the distal heme cavity |
| 3KRQ | 2010-05-26 | 2.25 | Crystal structure of the complex of lactoperoxidase with a potent inhibitor amino-triazole at 2.2a resolution |
| 5GH0 | 2016-06-29 | 2.3 | Crystal structure of the complex of bovine lactoperoxidase with mercaptoimidazole at 2.3 A resolution |
| 3BXI | 2008-03-25 | 2.3 | Structure of the complex of bovine lactoperoxidase with its catalyzed product hypothiocyanate ion at 2.3A resolution |
| 4NJB | 2013-11-27 | 2.31 | Crystal structure of the complex of lactoperoxidase from bovine with 3,3-oxydipyridine at 2.31 A resolution |
| 3GCJ | 2009-03-31 | 2.34 | Mode of ligand binding and assignment of subsites in mammalian peroxidases: crystal structure of lactoperoxidase complexes with acetyl salycylic acid, salicylhydroxamic acid and benzylhydroxamic acid |
| 2PT3 | 2007-05-22 | 2.34 | Crystal structure of bovine lactoperoxidase at 2.34 A resolution reveals multiple anion binding sites |
| 2QPK | 2007-08-07 | 2.34 | Crystal structure of the complex of bovine lactoperoxidase with salicylhydroxamic acid at 2.34 A resolution |
| 3TGY | 2011-09-21 | 2.35 | Crystal structure of the complex of Bovine Lactoperoxidase with Ascorbic acid at 2.35 A resolution |
| 3ERH | 2009-03-31 | 2.4 | First structural evidence of substrate specificity in mammalian peroxidases: Crystal structures of substrate complexes with lactoperoxidases from two different species |
| 4PNX | 2014-03-12 | 2.41 | Crystal structure of the complex of lactoperoxidase with bromo methane at 2.41 angstrom resolution |
| 3PY4 | 2011-01-05 | 2.42 | Crystal structure of bovine lactoperoxidase in complex with paracetamol at 2.4A resolution |
| 4GN6 | 2012-09-19 | 2.42 | Structure of paracetamol bound bovine lactoperoxidase at 2.45A resolution. |
| 3FNL | 2009-01-27 | 2.48 | Crystal Structure of the Complex of Buffalo Lactoperoxidase with Salicylhydroxamic Acid at 2.48 A Resolution |
| 3GC1 | 2009-03-31 | 2.5 | Crystal structure of bovine lactoperoxidase |
| 3GCL | 2009-03-31 | 2.5 | Mode of ligand binding and assignment of subsites in mammalian peroxidases: crystal structure of lactoperoxidase complexes with acetyl salycylic acid, salicylhydroxamic acid and benzylhydroxamic acid |
| 3ERI | 2009-03-31 | 2.5 | First structural evidence of substrate specificity in mammalian peroxidases: Crystal structures of substrate complexes with lactoperoxidases from two different species |
| 2QQT | 2007-08-07 | 2.5 | Crystal structure of the complex of bovine lactoperoxidase with acetyl salicylic acid at 2.5 A resolution |
| 2QRB | 2007-08-14 | 2.5 | Crystal structure of chloride saturated bovine lactoperoxidase at 2.5 A resolution shows multiple halide binding sites |
| 4GM7 | 2012-09-19 | 2.6 | Structure of cinnamic acid bound bovine lactoperoxidase at 2.6A resolution. |
| 3R5O | 2011-08-17 | 2.6 | Crystal structure of the complex of bovine lactoperoxidase with 4-allyl-2-methoxyphenol at 2.6 A resolution |
| 3UBA | 2011-12-28 | 2.65 | Crystal structure of the complex of bovine lactoperoxidase with p-hydroxycinnamic acid at 2.6 A resolution |
| 2PUM | 2007-05-22 | 2.7 | Crystal structure of bovine lactoperoxidase complex with catechol and iodide at 2.7 A resolution |
| 3FAQ | 2009-03-31 | 2.7 | Crystal structure of lactoperoxidase complex with cyanide |
| 3I6N | 2009-10-13 | 2.7 | Mode of Binding of the Tuberculosis Prodrug Isoniazid to Peroxidases: Crystal Structure of Bovine Lactoperoxidase with Isoniazid at 2.7 Resolution |
| 2GJM | 2006-05-02 | 2.75 | Crystal structure of Buffalo lactoperoxidase at 2.75A resolution |
| 2O86 | 2006-12-26 | 2.8 | Crystal structure of a ternary complex of buffalo lactoperoxidase with nitrate and iodide at 2.8 A resolution |
| 3GCK | 2009-03-31 | 2.9 | Mode of ligand binding and assignment of subsites in mammalian peroxidases: crystal structure of lactoperoxidase complexes with acetyl salycylic acid, salicylhydroxamic acid and benzylhydroxamic acid |
| 2NQX | 2006-11-21 | 2.95 | Crystal Structure of bovine lactoperoxidase with iodide ions at 2.9A resolution |
| 2IPS | 2006-10-24 | 3.1 | Crystal structure of a ternary complex of bovine lactoperoxidase with thiocyanate and iodide at 3.1 A resolution |
| 2Z5Z | 2007-08-14 | 3.5 | Crystal structure of the complex of buffalo Lactoperoxidase with fluoride ion at 3.5A resolution |