Hierarchical protein folding: asymmetric unfolding of an insulin analogue lacking the A7-B7 interchain disulfide bridge.


Abstract

The landscape paradigm of protein folding can enable preferred pathways on a funnel-like energy surface. Hierarchical preferences may be manifest as a nonrandom pathway of disulfide pairing. Stepwise stabilization of structural subdomains among on-pathway intermediates is proposed to underlie the disulfide pathway of proinsulin and related molecules. Here, effects of pairwise serine substitution of insulin's exposed interchain disulfide bridge (Cys(A7)-Cys(B7)) are characterized as a model of a late intermediate. Untethering cystine A7-B7 in an engineered monomer causes significantly more marked decreases in the thermodynamic stability and extent of folding than occur on pairwise substitution of internal cystine A6-A11 [Weiss, M. A., Hua, Q. X., Jia, W., Chu, Y. C., Wang, R. Y., and Katsoyannis, P. G. (2000) Biochemistry 39, 15429-15440]. Although substantially disordered and without significant biological activity, the untethered analogue contains a molten subdomain comprising cystine A20-B19 and a native-like cluster of hydrophobic side chains. Remarkably, A and B chains make unequal contributions to this folded moiety; the B chain retains native-like supersecondary structure, whereas the A chain is largely disordered. These observations suggest that the B subdomain provides a template to guide folding of the A chain. Stepwise organization of insulin-like molecules supports a hierarchic view of protein folding. Study holds ProTherm entries: 12271 Extra Details: funnel-like energy surface; disulfide pathway; supersecondary structure; hierarchic

Submission Details

ID: ApDMtf6N3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:43 p.m.

Version: 1

Publication Details
Hua QX;Nakagawa SH;Jia W;Hu SQ;Chu YC;Katsoyannis PG;Weiss MA,Biochemistry (2001) Hierarchical protein folding: asymmetric unfolding of an insulin analogue lacking the A7-B7 interchain disulfide bridge. PMID:11591149
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2H67 2006-07-18 NMR structure of human insulin mutant HIS-B5-ALA, HIS-B10-ASP PRO-B28-LYS, LYS-B29-PRO, 20 structures
1SF1 2004-03-30 NMR STRUCTURE OF HUMAN INSULIN under Amyloidogenic Condition, 15 STRUCTURES
1AI0 1997-11-12 R6 HUMAN INSULIN HEXAMER (NON-SYMMETRIC), NMR, 10 STRUCTURES
1MHJ 1995-10-15 SOLUTION STRUCTURE OF THE SUPERACTIVE MONOMERIC DES-[PHE(B25)] HUMAN INSULIN MUTANT. ELUCIDATION OF THE STRUCTURAL BASIS FOR THE MONOMERIZATION OF THE DES-[PHE(B25)] INSULIN AND THE DIMERIZATION OF NATIVE INSULIN
2HH4 2006-07-18 NMR structure of human insulin mutant GLY-B8-D-SER, HIS-B10-ASP PRO-B28-LYS, LYS-B29-PRO, 20 structures
2JV1 2007-12-11 NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures
2KJU 2010-06-16 NMR structure of human insulin mutant glu-b21-d-glu, his-b10 asp pro-b28-lys, lys-b29-pro, 20 structures
1HUI 1997-03-12 INSULIN MUTANT (B1, B10, B16, B27)GLU, DES-B30, NMR, 25 STRUCTURES
2M2N 2013-03-06 Structure of [L-HisB24] insulin analogue at pH 8.0
1A7F 1998-07-15 INSULIN MUTANT B16 GLU, B24 GLY, DES-B30, NMR, 20 STRUCTURES
2MPG 2014-06-11 Solution structure of the [AibB8,LysB28,ProB29]-insulin analogue
4IDW 2013-06-05 Polycrystalline T6 Bovine Insulin: Anisotropic Lattice Evolution and Novel Structure Refinement Strategy
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1JCO 2001-10-03 Solution structure of the monomeric [Thr(B27)->Pro,Pro(B28)->Thr] insulin mutant (PT insulin)
2JUU 2007-10-16 allo-ThrA3 DKP-insulin
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1T1Q 2004-08-10 NMR STRUCTURE OF HUMAN INSULIN MUTANT HIS-B10-ASP, VAL-B12-ABA, PRO-B28-LYS, LYS-B29-PRO, 15 STRUCTURES
1BZV 1999-05-18 [D-ALAB26]-DES(B27-B30)-INSULIN-B26-AMIDE A SUPERPOTENT SINGLE-REPLACEMENT INSULIN ANALOGUE, NMR, MINIMIZED AVERAGE STRUCTURE
2HHO 2006-07-18 NMR structure of human insulin mutant GLY-B8-SER, HIS-B10-ASP PRO-B28-LYS, LYS-B29-PRO, 20 structures
2M2O 2013-03-06 Structure of [D-HisB24] insulin analogue at pH 1.9
2AIY 2000-02-28 R6 HUMAN INSULIN HEXAMER (SYMMETRIC), NMR, 20 STRUCTURES
2JMN 2006-12-05 NMR structure of human insulin mutant His-B10-Asp, Pro-B28-Lys, Lys-B29-Pro, 20 structures
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2KQP 2010-01-26 NMR Structure of Proinsulin
2MVD 2014-12-10 Solution structure of [GlnB22]-insulin mutant at pH 1.9
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1K3M 2001-10-17 NMR STRUCTURE OF HUMAN INSULIN MUTANT ILE-A2-ALA, HIS-B10-ASP, PRO-B28-LYS, LYS-B29-PRO, 15 STRUCTURES
2N2V 2016-02-03 Solution structure of [B26-B29 triazole cross-linked]-insulin analogue at pH 1.9
1SJU 1998-03-18 MINI-PROINSULIN, SINGLE CHAIN INSULIN ANALOG MUTANT: DES B30, HIS(B 10)ASP, PRO(B 28)ASP AND PEPTIDE BOND BETWEEN LYS B 29 AND GLY A 1, NMR, 20 STRUCTURES
2N2X 2016-02-03 Solution structure of [GlyB24,B27-B29 triazole cross-linked]-insulin analogue at pH 1.9
1VKT 1997-04-01 HUMAN INSULIN TWO DISULFIDE MODEL, NMR, 10 STRUCTURES
1T0C 2005-08-16 Solution Structure of Human Proinsulin C-Peptide
5MIZ 2017-01-25 MD ensemble of bovine insulin
2RN5 2008-10-28 Humal Insulin Mutant B31Lys-B32Arg
1HIS 1994-01-31 Structure and dynamics of des-pentapeptide-insulin in solution: the molten-globule hypothesis.
1SJT 1998-03-18 MINI-PROINSULIN, TWO CHAIN INSULIN ANALOG MUTANT: DES B30, HIS(B 10)ASP, PRO(B 28)ASP, NMR, 20 STRUCTURES
4AIY 2000-02-28 R6 HUMAN INSULIN HEXAMER (SYMMETRIC), NMR, 'GREEN' SUBSTATE, AVERAGE STRUCTURE
1EFE 2000-03-17 AN ACTIVE MINI-PROINSULIN, M2PI
2KQQ 2010-11-24 NMR structure of human insulin mutant gly-b8-d-ala, his-b10-asp, pro-b28-lys, lys-b29-pro, 20 structures
1MHI 1995-10-15 THREE-DIMENSIONAL SOLUTION STRUCTURE OF AN INSULIN DIMER. A STUDY OF THE B9(ASP) MUTANT OF HUMAN INSULIN USING NUCLEAR MAGNETIC RESONANCE DISTANCE GEOMETRY AND RESTRAINED MOLECULAR DYNAMICS
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5E7W 2016-10-12 0.95 X-ray Structure of Human Recombinant 2Zn insulin at 0.92 Angstrom
5HQI 2017-01-25 0.97 Insulin with proline analog HzP at position B28 in the T2 state
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1MSO 2003-03-04 1.0 T6 Human Insulin at 1.0 A Resolution
4FKA 2013-02-13 1.08 High resolution structure of the manganese derivative of insulin
3TT8 2011-10-19 1.12 Crystal Structure Analysis of Cu Human Insulin Derivative
3W7Z 2013-07-03 1.15 1.15A structure of human 2Zn insulin at 293K
4IHN 2013-12-25 1.16 High Resolution Insulin by Langmuir-Blodgett Modified Hanging Drop Vapour Diffusion
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1G7A 2001-08-03 1.2 1.2 A structure of T3R3 human insulin at 100 K
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6GV0 2019-07-03 1.26 Insulin glulisine
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1G7B 2001-08-03 1.3 1.3 A STRUCTURE OF T3R3 HUMAN INSULIN AT 100 K
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1PID 1996-12-07 1.3 BOVINE DESPENTAPEPTIDE INSULIN
4E7U 2012-04-04 1.3 The structure of T3R3 bovine insulin
3BXQ 2008-05-20 1.3 The structure of a mutant insulin uncouples receptor binding from protein allostery. An electrostatic block to the TR transition
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1BEN 1996-07-11 1.4 INSULIN COMPLEXED WITH 4-HYDROXYBENZAMIDE
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4EY9 2013-05-01 1.47 Human Insulin
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5D54 2016-01-13 1.5 In meso X-ray crystallography structure of insulin at 100 K
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1ZNI 1998-01-28 1.5 INSULIN
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2C8R 2006-03-08 1.5 insuline(60sec) and UV laser excited fluorescence
5D53 2016-01-13 1.5 In meso in situ serial X-ray crystallography structure of insulin at 100 K
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4F1B 2013-05-08 1.59 Human Insulin
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1DEI 1997-06-16 1.6 DESHEPTAPEPTIDE (B24-B30) INSULIN
1TRZ 1994-01-31 1.6 CRYSTALLOGRAPHIC EVIDENCE FOR DUAL COORDINATION AROUND ZINC IN THE T3R3 HUMAN INSULIN HEXAMER
1ZEG 1998-07-15 1.6 STRUCTURE OF B28 ASP INSULIN IN COMPLEX WITH PHENOL
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3ZU1 2011-08-03 1.6 Structure of LysB29(Nepsilon omega-carboxyheptadecanoyl) des(B30) Human Insulin
1GUJ 2002-03-08 1.62 Insulin at pH 2: structural analysis of the conditions promoting insulin fibre formation.
4F1D 2013-05-08 1.64 Human Insulin
4F51 2013-05-15 1.64 Human Insulin
4F4T 2013-05-15 1.64 Human Insulin
4F4V 2013-05-15 1.64 Human Insulin
4F1G 2013-05-08 1.64 Human insulin
1SDB 1997-02-12 1.65 PORCINE DESB1-2 DESPENTAPEPTIDE(B26-B30) INSULIN
6QQ7 2019-02-27 1.65 Bovine insulin at ambient pressure
4IYD 2014-02-12 1.66 Insulin glargine crystal structure 1
4EX1 2013-05-01 1.66 Human Insulin
4F0O 2013-05-08 1.67 Human Insulin
3UTQ 2012-01-25 1.67 Human HLA-A*0201-ALWGPDPAAA
4F1F 2013-05-08 1.68 Human Insulin
4F8F 2013-05-22 1.68 Human Insulin
5C0D 2016-05-04 1.68 HLA-A02 carrying AQWGPDPAAA
4F0N 2013-05-08 1.68 Human Insulin
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1B2D 2003-04-08 1.7 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.35 COORDINATES)
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4F1C 2013-05-08 1.7 Human Insulin
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1EV3 2000-12-04 1.78 Structure of the rhombohedral form of the M-cresol/insulin R6 hexamer
4EWZ 2013-05-01 1.79 Human Insulin
5CO6 2015-08-26 1.8 Crystal structure of human zinc insulin at pH 6.5
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1XDA 1997-07-07 1.8 STRUCTURE OF INSULIN
2CEU 2006-03-03 1.8 Despentapeptide insulin in acetic acid (pH 2)
4F1A 2013-05-08 1.8 Human Insulin
4IYF 2014-02-12 1.8 Insulin glargine crystal structure 2
1B19 2003-04-08 1.8 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.80 COORDINATES)
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1B2G 2003-04-08 1.8 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 9.00 COORDINATES)
4UNG 2014-10-15 1.81 Human insulin B26Asn mutant crystal structure
6H3M 2019-08-14 1.82 The crystal structure of a human seleno-insulin analog
4Z77 2015-06-24 1.85 Weak TCR binding to an unstable insulin epitope drives type 1 diabetes
3I40 2010-01-05 1.85 Human insulin
3INC 2010-08-18 1.85 Crystal structure of human insulin with Ni+2 complex
4A7E 2011-11-30 1.86 X-ray crystal structure of porcine insulin flash-cooled at high pressure
4EX0 2013-05-01 1.86 Human Insulin
4GBN 2013-06-12 1.87 Crystal structure of aspart insulin at pH 6.5
5MT9 2017-04-05 1.88 Human insulin in complex with serotonin and arginine
4Z76 2015-06-24 1.88 Weak TCR binding to an unstable insulin epitope drives type 1 diabetes
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1EV6 2000-12-04 1.9 Structure of the monoclinic form of the M-cresol/insulin R6 hexamer
5FB6 2016-06-15 1.9 Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
4M4H 2014-01-15 1.9 Radiation damage study of Cu T6-insulin - 0.06 MGy
5BPO 2016-02-03 1.9 Human insulin with intra-chain chemical crosslink between modified B27 and B29
1DPH 1993-01-15 1.9 CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11
1TYL 1994-09-30 1.9 THE STRUCTURE OF A COMPLEX OF HEXAMERIC INSULIN AND 4'-HYDROXYACETANILIDE
1TYM 1994-09-30 1.9 THE STRUCTURE OF A COMPLEX OF HEXAMERIC INSULIN AND 4'-HYDROXYACETANILIDE
4M4I 2014-01-15 1.9 Radiation damage study of Cu T6-insulin - 0.12 MGy
1B2F 2003-04-08 1.9 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.98 COORDINATES)
4M4F 2014-01-15 1.9 Radiation damage study of Cu T6-insulin - 0.01 MGy
4M4J 2014-01-15 1.9 Radiation damage study of Cu T6-insulin - 0.30 MGy
1HTV 2001-05-23 1.9 CRYSTAL STRUCTURE OF DESTRIPEPTIDE (B28-B30) INSULIN
1B2E 2003-04-08 1.9 PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.50 COORDINATES)
3MTH 1996-01-29 1.9 X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL
3ZQR 2011-08-24 1.9 NMePheB25 insulin analogue crystal structure
1ZEI 1999-02-16 1.9 CROSS-LINKED B28 ASP INSULIN
3KQ6 2010-02-23 1.9 Enhancing the Therapeutic Properties of a Protein by a Designed Zinc-Binding Site, Structural principles of a novel long-acting insulin analog
2WS4 2010-02-09 1.9 Semi-synthetic analogue of human insulin ProB26-DTI in monomer form
1CPH 1993-01-15 1.9 CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11
1EVR 2000-12-04 1.9 The structure of the resorcinol/insulin R6 hexamer
5CO9 2015-08-26 1.92 Crystal structure of human zinc insulin at pH 6.5
2C8Q 2006-03-08 1.95 insuline(1sec) and UV laser excited fluorescence
1OS3 2003-07-29 1.95 Dehydrated T6 human insulin at 100 K
3ZS2 2011-08-31 1.97 TyrB25,NMePheB26,LysB28,ProB29-insulin analogue crystal structure
5UU4 2018-02-21 1.97 Insulin with proline analog ThioP at position B28 in the R6 state
2OM1 2007-12-04 1.97 Structure of human insulin in presence of thiocyanate at pH 6.5
3U4N 2012-04-11 1.98 A novel covalently linked insulin dimer
4EFX 2013-03-06 1.98 Highly biologically active insulin with additional disulfide bond
3P2X 2011-11-23 2.0 Insulin fibrillation is the Janus face of induced fit. A chiaral clamp stabilizes the native state at the expense of activity
3FHP 2009-10-20 2.0 A neutron crystallographic analysis of a porcine 2Zn insulin at 2.0 A resolution
1BPH 1993-01-15 2.0 CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11
1APH 1993-01-15 2.0 CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11
1J73 2001-05-30 2.0 Crystal structure of an unstable insulin analog with native activity.
7INS 1994-01-31 2.0 STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z
1ZNJ 1998-01-28 2.0 INSULIN, MONOCLINIC CRYSTAL FORM
4FG3 2013-06-12 2.0 Crystal Structure Analysis of the Human Insulin
2OMQ 2007-01-30 2.0 VEALYL peptide derived from human insulin chain B, residues 12-17
2QIU 2008-02-26 2.0 Structure of Human Arg-Insulin
3V19 2012-12-12 2.0 Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health
6INS 1994-01-31 2.0 X-RAY ANALYSIS OF THE SINGLE CHAIN B29-A1 PEPTIDE-LINKED INSULIN MOLECULE. A COMPLETELY INACTIVE ANALOGUE
2W44 2009-12-22 2.0 Structure DeltaA1-A4 insulin
1Q4V 2003-08-19 2.0 CRYSTAL STRUCTURE OF ALLO-ILEA2-INSULIN, AN INACTIVE CHIRAL ANALOGUE: IMPLICATIONS FOR THE MECHANISM OF RECEPTOR
1IZB 1993-10-31 2.0 ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN
2R36 2008-09-02 2.0 Crystal structure of ni human ARG-insulin
1QIZ 1999-06-22 2.0 HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR COMPLEXED WITH RESORCINOL
4AKJ 2013-01-09 2.01 Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
5MT3 2017-04-05 2.02 Human insulin in complex with serotonin and arginine
2OM0 2007-12-04 2.05 Structure of human insulin in presence of urea at pH 6.5
3Q6E 2011-04-20 2.05 Human insulin in complex with cucurbit[7]uril
5USS 2018-02-21 2.06 Insulin with proline analog PiP at position B28 in the R6 state
2R35 2008-09-02 2.08 Crystal structure of RB human arg-insulin
1W8P 2005-02-03 2.08 Structural properties of the B25Tyr-NMe-B26Phe insulin mutant.
6QRK 2019-03-06 2.1 High pressure structure of bovine insulin (200 MPa)
2WS0 2010-02-09 2.1 Semi-synthetic analogue of human insulin NMeAlaB26-insulin at pH 7.5
3T2A 2011-12-28 2.1 TMAO-grown cubic insulin (porcine)
6QRH 2019-02-27 2.15 High pressure structure of bovine insulin (100 MPa)
6QQG 2019-03-06 2.15 High pressure structure of bovine insulin (60 MPa)
2WRV 2010-02-09 2.15 Semi-synthetic highly active analogue of human insulin NMeHisB26-DTI- NH2
3V1G 2012-12-12 2.2 Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health
6GNQ 2019-06-12 2.2 Monoclinic crystalline form of human insulin, complexed with meta-cresol
5HPU 2017-01-25 2.2 Insulin with proline analog HyP at position B28 in the R6 state
4EWX 2013-05-01 2.2 Human Insulin
2VK0 2008-09-16 2.2 Crystal structure form ultalente insulin microcrystals
5MAM 2017-04-05 2.2 Human insulin in complex with serotonin
2G56 2006-10-24 2.2 crystal structure of human insulin-degrading enzyme in complex with insulin B chain
3IR0 2010-09-01 2.2 Crystal Structure of Human Insulin complexed with Cu+2 metal ion
2OMI 2007-03-27 2.24 Structure of human insulin cocrystallized with protamine
2G54 2006-10-24 2.25 Crystal structure of Zn-bound human insulin-degrading enzyme in complex with insulin B chain
6CK2 2018-06-13 2.25 Insulin analog containing a YB26W mutation
1OS4 2003-07-29 2.25 Dehydrated T6 human insulin at 295 K
5UU3 2018-02-21 2.25 Insulin with proline analog DfP at position B28 in the R6 state
2R34 2008-09-02 2.25 Crystal structure of MN human arg-insulin
2A3G 2005-10-25 2.25 The structure of T6 bovine insulin
4AK0 2013-01-09 2.28 Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
5LIS 2016-08-03 2.29 Insulin solved by Native SAD from a dataset collected in one second
3ROV 2012-05-02 2.3 Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus
4BS3 2013-07-17 2.3 Bovin insulin structure determined by in situ crystal analysis and sulfur-SAD phasing at room temperature
3P33 2011-11-23 2.3 Insulin fibrillation is the Janus face of induced fit. A chiral clamp stabilizes the native state at the expense of activity
4Z78 2015-06-24 2.3 Weak TCR binding to an unstable insulin epitope drives type 1 diabetes
1LPH 1996-06-20 2.3 LYS(B28)PRO(B29)-HUMAN INSULIN
5EMS 2016-11-16 2.3 Crystal Structure of an iodinated insulin analog
1MPJ 1996-01-29 2.3 X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL
4EWW 2013-05-01 2.3 Human Insulin
1XW7 2005-04-12 2.3 Diabetes-Associated Mutations in Human Insulin: Crystal Structure and Photo-Cross-Linking Studies of A-Chain Variant Insulin Wakayama
1QIY 1999-06-22 2.3 HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR COMPLEXED WITH PHENOL
4WDI 2015-06-24 2.31 Weak TCR binding to an unstable insulin epitope drives type 1 diabetes
3WS3 2014-03-26 2.34 Crystal Structure of H-2D in complex with an insulin derived peptide
4GBK 2013-06-12 2.4 Crystal structure of aspart insulin at pH 8.5
1JK8 2001-08-22 2.4 Crystal structure of a human insulin peptide-HLA-DQ8 complex
1QJ0 1999-06-22 2.4 HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR
5D5E 2016-01-13 2.41 In meso in situ serial X-ray crystallography structure of insulin by sulfur-SAD at 100 K
5URU 2018-02-21 2.41 Insulin with proline analog DhP at position B28 in the R6 state
2WRW 2010-02-09 2.41 Semi-synthetic highly active analogue of human insulin D-ProB26-DTI- NH2
2ZPP 2008-10-28 2.5 Neutron crystal structure of cubic insulin at pD9
4GBI 2013-06-12 2.5 Crystal structure of aspart insulin at pH 6.5
1JCA 2001-06-20 2.5 Non-standard Design of Unstable Insulin Analogues with Enhanced Activity
2INS 1982-08-05 2.5 THE STRUCTURE OF DES-PHE B1 BOVINE INSULIN
4Y19 2015-09-23 2.5 immune complex
1B9E 1999-11-17 2.5 HUMAN INSULIN MUTANT SERB9GLU
1WAV 1997-02-28 2.5 CRYSTAL STRUCTURE OF FORM B MONOCLINIC CRYSTAL OF INSULIN
1IZA 1993-10-31 2.5 ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN
4GBL 2013-06-12 2.5 Crystal structure of aspart insulin at pH 8.5
3JSD 2010-09-15 2.5 Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus
2ZP6 2008-07-22 2.56 Crystal structure of Bovine Insulin (Hexameric form)
2WS7 2010-02-09 2.59 Semi-synthetic analogue of human insulin ProB26-DTI
2WBY 2009-03-24 2.6 Crystal structure of human insulin-degrading enzyme in complex with insulin
3UTT 2012-01-25 2.6 1E6-A*0201-ALWGPDPAAA Complex, Triclinic
2EFA 2008-01-22 2.7 Neutron crystal structure of cubic insulin at pD6.6
3UTS 2012-01-25 2.71 1E6-A*0201-ALWGPDPAAA Complex, Monoclinic
4UNH 2014-10-15 2.75 Human insulin B26Gly mutant crystal structure
5WDM 2017-11-15 2.8 An ultra-stable single-chain insulin analog resists thermal inactivation and exhibits biological signaling duration equivalent to the native protein
2WC0 2009-03-24 2.8 crystal structure of human insulin degrading enzyme in complex with iodinated insulin
5HYJ 2016-05-04 3.06 1E6 TCR in Complex with HLA-A02 carrying AQWGPDPAAA
6HN5 2018-11-21 3.2 Leucine-zippered human insulin receptor ectodomain with single bound insulin - 'upper' membrane-distal part
5CJO 2016-07-20 3.29 Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin
4OGA 2014-08-27 3.5 Insulin in complex with Site 1 of the human insulin receptor
6B3Q 2017-11-22 3.7 Cryo-EM structure of human insulin degrading enzyme in complex with insulin
6B70 2017-12-27 3.7 Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain and insulin
6BFC 2017-12-27 3.7 Cryo-EM structure of human insulin degrading enzyme in complex with insulin
3W11 2013-01-09 3.9 Insulin receptor ectodomain construct comprising domains L1-CR in complex with human insulin, Alpha-CT peptide(704-719) and FAB 83-7
5WOB 2018-04-18 3.95 Crystal Structure Analysis of Fab1-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin
4Y1A 2015-09-23 4.0 immune complex
3W12 2013-01-09 4.3 Insulin receptor ectodomain construct comprising domains L1-CR in complex with high-affinity insulin analogue [D-PRO-B26]-DTI-NH2, alpha-CT peptide(704-719) and FAB 83-7
3W13 2013-01-09 4.3 Insulin receptor ectodomain construct comprising domains L1-CR in complex with high-affinity insulin analogue [D-PRO-B26]-DTI-NH2, alphact peptide(693-719) and FAB 83-7
6CE9 2018-03-14 4.3 Insulin Receptor ectodomain in complex with two insulin molecules
5KQV 2017-07-19 4.4 Insulin receptor ectodomain construct comprising domains L1,CR,L2, FnIII-1 and alphaCT peptide in complex with bovine insulin and FAB 83-14 (REVISED STRUCTURE)
6CEB 2018-03-14 4.7 Insulin Receptor ectodomain in complex with two insulin molecules - C1 symmetry
6CE7 2018-03-14 7.4 Insulin Receptor ectodomain in complex with one insulin molecule

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
547.8 B,D,F,H,J,L Insulin P01338 INS2_BATSP
550.2 B,D,F,H,J,L Insulin P68987 INS_PETMA
550.2 B,D,F,H,J,L Insulin P68988 INS_LAMFL
555.6 B,D,F,H,J,L Insulin C0HJI7 INS1_HUSDA
553.8 B,D,F,H,J,L Insulin C0HJI3 INS2_KATPE
553.8 B,D,F,H,J,L Insulin P09476 INS_ATRSP
553.8 B,D,F,H,J,L Insulin Q9W7R2 INS_VERMO
553.8 B,D,F,H,J,L Insulin P09477 INS_PLAFE
553.8 B,D,F,H,J,L Insulin P04667 INS_ONCKE
555.6 B,D,F,H,J,L Insulin P01340 INS_KATPE
558.6 B,D,F,H,J,L Insulin P83770 INSL_VIGUN
555.6 B,D,F,H,J,L Insulin P12707 INS2_XENLA
577.2 B,D,F,H,J,L Insulin C0HJI8 INS2_HUSDA
579.6 B,D,F,H,J,L Insulin P21563 INS_RODSP
600.0 B,D,F,H,J,L Insulin P81423 INS_ACIGU
557.4 B,D,F,H,J,L Insulin P12706 INS1_XENLA
577.8 B,D,F,H,J,L Insulin P69048 INS_TRASC
577.8 B,D,F,H,J,L Insulin P69047 INS_TRADO
577.8 B,D,F,H,J,L Insulin P67969 INS_STRCA
577.8 B,D,F,H,J,L Insulin P67968 INS_MELGA
577.8 B,D,F,H,J,L Insulin P67970 INS_CHICK
1149.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01323 INS2_RAT
1149.6000000000001 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01326 INS2_MOUSE
578.4 B,D,F,H,J,L Insulin P51463 INS_SELRF
578.4 B,D,F,H,J,L Insulin P68243 INS_CAIMO
578.4 B,D,F,H,J,L Insulin P68245 INS_ANSAN
578.4 B,D,F,H,J,L Insulin P01333 INS_ANAPL
1143.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P18109 INS_DIDVI
559.8 B,D,F,H,J,L Insulin P67971 INS_SAISC
559.8 B,D,F,H,J,L Insulin P67972 INS_AOTTR
1122.6 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01320 INS_CAMDR
1131.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01324 INS_ACOCA
600.0 B,D,F,H,J,L Insulin P01318 INS_SHEEP
1171.2 A,B,C,D,E,F,G,H,I,J,K,L Insulin Q62587 INS_PSAOB
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01315 INS_PIG
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P67974 INS_PHYMC
1171.2 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01310 INS_HORSE
600.0 B,D,F,H,J,L Insulin P06306 INS_FELCA
600.0 B,D,F,H,J,L Insulin P01319 INS_CAPHI
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01321 INS_CANLF
1143.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01317 INS_BOVIN
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P67973 INS_BALPH
1143.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01314 INS_BALBO
1180.2 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01311 INS_RABIT
1180.2 A,B,C,D,E,F,G,H,I,J,K,L Insulin Q91XI3 INS_ICTTR
1151.4000000000003 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01313 INS_CRILO
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin Q8HXV2 INS_PONPY
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P30410 INS_PANTR
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P30406 INS_MACFA
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01308 INS_HUMAN
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin Q6YK33 INS_GORGO
1143.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P01316 INS_ELEMA
1200.0 A,B,C,D,E,F,G,H,I,J,K,L Insulin P30407 INS_CHLAE
600.0 B,D,F,H,J,L Insulin F8WCM5 INSR2_HUMAN
543.0 A,C,E,G,I,K Insulin P01327 INS_CHICH
571.2 A,C,E,G,I,K Insulin P01322 INS1_RAT
571.2 A,C,E,G,I,K Insulin P01325 INS1_MOUSE