Stability studies on derivatives of the bovine pancreatic trypsin inhibitor.
Abstract
Gibbs energy, enthalpy, and entropy data were determined for two selectively modified analogues of bovine pancreatic trypsin inhibitor (BPTI) to provide a model free set of thermodynamic parameters that characterize (a) the energetic and entropic contributions of the 14-38 disulfide bridge and (b) the variation of the overall stability resulting from the introduction of two negative charges into the positions 14 and 38. The two BPTI analogues studied were BPTI having Cys-14 and Cys-38 carboxymethylated (BPTI-RCOM) and BPTI having Cys-14 and Cys-38 carboxamidomethylated (BPTI-RCAM). They were obtained from native BPTI by reduction, followed by modification of the sulfhydryl groups with iodoacetic acid or iodoacetamide, respectively. The temperature dependence of all thermodynamic parameters of BPTI is drastically altered in the absence of the third disulfide bridge. Even the apparently minute difference of two dissociable carboxyl groups instead of uncharged amide groups in positions 14 and 38 has surprisingly large effects on the temperature dependence of the stabilization enthalpy. The Gibbs energy of BPTI at pH 2, 25 degrees C, decreases by approximately 70% when the 14-38 disulfide bond is cleaved. BPTI-RCOM is more stable than BPTI-RCAM in the whole pH range studied. The difference of -4 kJ/mol at pH 2, 25 degrees C, is reduced to -2.7 kJ/mol at pH 5, 25 degrees C. This finding demonstrates that the presence of two negative charges reduces the higher stability of BPTI-RCOM slightly; however, the overall effect of the two charges is still a stabilization.(ABSTRACT TRUNCATED AT 250 WORDS) Study holds ProTherm entries: 3887, 3888 Extra Details: entropic contribution; negative charges; disulfide bridge;,thermodynamic parameters
Submission Details
ID: Afpz7XLJ3
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:23 p.m.
Version: 1
Publication Details
Schwarz H;Hinz HJ;Mehlich A;Tschesche H;Wenzel HR,Biochemistry (1987) Stability studies on derivatives of the bovine pancreatic trypsin inhibitor. PMID:2443162Additional Information
Study Summary
| Number of data points | 2 |
| Proteins | Pancreatic trypsin inhibitor ; Pancreatic trypsin inhibitor |
| Unique complexes | 1 |
| Assays/Quantities/Protocols | Experimental Assay: dG pH:5.0 ; Experimental Assay: dG pH:2.0 |
| Libraries | Mutations for sequence RPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGA |
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 1UUB | 2004-01-29 | Solution structure of a truncated bovine pancreatic trypsin inhibitor mutant, 3-58 BPTI (K15R, R17A, R42S) | |
| 1JV8 | 2001-09-12 | NMR Structure of BPTI Mutant G37A | |
| 1PIT | 1994-01-31 | DETERMINATION OF A HIGH-QUALITY NUCLEAR MAGNETIC RESONANCE SOLUTION STRUCTURE OF THE BOVINE PANCREATIC TRYPSIN INHIBITOR AND COMPARISON WITH THREE CRYSTAL STRUCTURES | |
| 1OA5 | 2003-08-28 | The solution structure of bovine pancreatic trypsin inhibitor at high pressure | |
| 1K09 | 2002-07-10 | Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein | |
| 1LD5 | 2002-09-11 | STRUCTURE OF BPTI MUTANT A16V | |
| 1JV9 | 2001-09-12 | NMR Structure of BPTI Mutant G37A | |
| 1UUA | 2004-01-29 | Solution structure of a truncated bovine pancreatic trypsin inhibitor, 3-58 BPTI. | |
| 1LD6 | 2002-09-11 | STRUCTURE OF BPTI_8A MUTANT | |
| 3OTJ | 2011-01-26 | A Crystal Structure of Trypsin Complexed with BPTI (Bovine Pancreatic Trypsin Inhibitor) by X-ray/Neutron Joint Refinement | |
| 1OA6 | 2003-08-28 | The solution structure of bovine pancreatic trypsin inhibitor at high pressure | |
| 1G6X | 2001-05-09 | 0.86 | ULTRA HIGH RESOLUTION STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR (BPTI) MUTANT WITH ALTERED BINDING LOOP SEQUENCE |
| 1K6U | 2001-12-19 | 1.0 | Crystal Structure of Cyclic Bovine Pancreatic Trypsin Inhibitor |
| 5PTI | 1984-10-29 | 1.0 | STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR. RESULTS OF JOINT NEUTRON AND X-RAY REFINEMENT OF CRYSTAL FORM II |
| 2ZVX | 2009-10-13 | 1.09 | Structure of a BPTI-[5,55] variant containing Gly/Val at the 14/38th positions |
| 2ZJX | 2008-10-21 | 1.09 | Bovine pancreatic trypsin inhibitor (BPTI) containing only the [5,55] disulfide bond |
| 1BPI | 1995-06-03 | 1.09 | THE STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR AT 125K: DEFINITION OF CARBOXYL-TERMINAL RESIDUES GLYCINE-57 AND ALANINE-58 |
| 5XX3 | 2018-07-04 | 1.12 | A BPTI-[5,55] variant with C14GA38G mutations |
| 5XX2 | 2018-07-04 | 1.12 | A BPTI-[5,55] variant with C14GA38L mutations |
| 4Y0Y | 2015-06-24 | 1.25 | Trypsin in complex with with BPTI |
| 5XX8 | 2018-07-04 | 1.3 | Hetero-micro-seeding: Crystal structure of BPTI-[555]C14GA38I variant using micro-seeds from -C14GA38L variant |
| 3P95 | 2011-08-31 | 1.3 | Human mesotrypsin complexed with bovine pancreatic trypsin inhibitor variant (BPTI-K15R/R17D) |
| 4Y11 | 2015-06-24 | 1.3 | Trypsin in complex with with BPTI mutant (2S)-2-amino-4,4,4-trifluorobutanoic acid |
| 3WNY | 2014-06-18 | 1.3 | A simplified BPTI variant with poly Lys amino acid tag (C3K) at the C-terminus |
| 5XX6 | 2018-07-04 | 1.31 | Hetero-micro-seeding: Crystal structure of BPTI-[5,55]C14GA38I variant using micro-seeds from -C14GA38G variant |
| 1P2J | 2004-04-20 | 1.35 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 4Y10 | 2015-06-24 | 1.37 | Trypsin in complex with with BPTI mutant (2S)-2-amino-4,4-difluorobutanoic acid |
| 4Y0Z | 2015-06-24 | 1.37 | Trypsin in complex with with BPTI mutant AMINOBUTYRIC ACID |
| 5XX5 | 2018-07-04 | 1.38 | A BPTI-[5,55] variant with C14GA38I mutations |
| 5XX7 | 2018-07-04 | 1.38 | Hetero-micro-seeding: Crystal structure of BPTI-[5,55]C14GA38I variant using micro-seeds from -C14GA38I variant |
| 4DG4 | 2012-09-12 | 1.4 | Human mesotrypsin-S39Y complexed with bovine pancreatic trypsin inhibitor (BPTI) |
| 2R9P | 2007-12-11 | 1.4 | Human mesotrypsin complexed with bovine pancreatic trypsin inhibitor(BPTI) |
| 1QLQ | 1999-10-05 | 1.42 | Bovine Pancreatic Trypsin Inhibitor (BPTI) Mutant with Altered Binding Loop Sequence |
| 3FP8 | 2009-02-17 | 1.46 | Anionic trypsin variant S195A in complex with bovine pancreatic trypsin inhibitor (BPTI) determined to the 1.46 A resolution limit |
| 3FP7 | 2009-02-17 | 1.46 | Anionic trypsin variant S195A in complex with bovine pancreatic trypsin inhibitor (BPTI) cleaved at the scissile bond (LYS15-ALA16) determined to the 1.46 A resolution limit |
| 2RA3 | 2007-12-11 | 1.46 | Human cationic trypsin complexed with bovine pancreatic trypsin inhibitor (BPTI) |
| 3FP6 | 2009-02-17 | 1.49 | Anionic trypsin in complex with bovine pancreatic trypsin inhibitor (BPTI) determined to the 1.49 A resolution limit |
| 4PTI | 1983-01-18 | 1.5 | THE GEOMETRY OF THE REACTIVE SITE AND OF THE PEPTIDE GROUPS IN TRYPSIN, TRYPSINOGEN AND ITS COMPLEXES WITH INHIBITORS |
| 1F7Z | 2001-07-04 | 1.55 | RAT TRYPSINOGEN K15A COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 2FI5 | 2006-01-24 | 1.58 | Crystal structure of a BPTI variant (Cys38->Ser) in complex with trypsin |
| 2FI4 | 2006-01-24 | 1.58 | Crystal structure of a BPTI variant (Cys14->Ser) in complex with trypsin |
| 2FI3 | 2006-01-24 | 1.58 | Crystal structure of a BPTI variant (Cys14->Ser, Cys38->Ser) in complex with trypsin |
| 3P92 | 2011-08-31 | 1.6 | Human mesotrypsin complexed with bovine pancreatic trypsin inhibitor variant (BPTI-K15R/R17G) |
| 5JB5 | 2017-04-19 | 1.6 | A simplified BPTI variant containing 22 alanines out of 58 residues |
| 7PTI | 1991-04-15 | 1.6 | STRUCTURAL EFFECTS INDUCED BY REMOVAL OF A DISULFIDE BRIDGE. THE X-RAY STRUCTURE OF THE C30A(SLASH)C51A MUTANT OF BASIC PANCREATIC TRYPSIN INHIBITOR AT 1.6 ANGSTROMS |
| 1AAL | 1993-10-31 | 1.6 | STRUCTURAL EFFECTS INDUCED BY MUTAGENESIS AFFECTED BY CRYSTAL PACKING FACTORS: THE STRUCTURE OF A 30-51 DISULFIDE MUTANT OF BASIC PANCREATIC TRYPSIN INHIBITOR |
| 1P2K | 2004-04-20 | 1.6 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 2FTL | 2006-02-14 | 1.62 | Crystal structure of trypsin complexed with BPTI at 100K |
| 1D0D | 2000-09-09 | 1.62 | CRYSTAL STRUCTURE OF TICK ANTICOAGULANT PROTEIN COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 1F5R | 2001-07-04 | 1.65 | RAT TRYPSINOGEN MUTANT COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 2FTM | 2006-02-14 | 1.65 | Crystal structure of trypsin complexed with the BPTI variant (Tyr35->Gly) |
| 1P2I | 2004-04-20 | 1.65 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 5XX4 | 2018-07-04 | 1.67 | A BPTI-[5,55] variant with C14GA38K mutations |
| 1T8O | 2005-03-08 | 1.7 | CRYSTAL STRUCTURE OF THE P1 TRP BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX |
| 1FY8 | 2000-11-08 | 1.7 | CRYSTAL STRUCTURE OF THE DELTAILE16VAL17 RAT ANIONIC TRYPSINOGEN-BPTI COMPLEX |
| 3LDJ | 2010-09-15 | 1.7 | Crystal structure of aprotinin in complex with sucrose octasulfate: unusual interactions and implication for heparin binding |
| 6PTI | 1987-10-16 | 1.7 | STRUCTURE OF FORM III CRYSTALS OF BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 4WWY | 2015-07-22 | 1.7 | human cationic trypsin G193R mutant in complex with bovine pancreatic trypsin inhibitor |
| 1YLC | 2006-04-25 | 1.7 | Trypsin/BPTI complex mutant |
| 1YLD | 2006-04-25 | 1.7 | Trypsin/BPTI complex mutant |
| 1YKT | 2006-04-25 | 1.7 | Trypsin/Bpti complex mutant |
| 3TGK | 2001-07-04 | 1.7 | TRYPSINOGEN MUTANT D194N AND DELETION OF ILE 16-VAL 17 COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR (BPTI) |
| 6F1F | 2018-07-18 | 1.72 | The methylene thioacetal BPTI (Bovine Pancreatic Trypsin Inhibitor) mutant structure |
| 1T8L | 2005-03-08 | 1.75 | CRYSTAL STRUCTURE OF THE P1 MET BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX |
| 1T8N | 2005-03-08 | 1.75 | CRYSTAL STRUCTURE OF THE P1 THR BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX |
| 3BTH | 2000-03-13 | 1.75 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 1P2M | 2004-04-20 | 1.75 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 3BTM | 2000-03-13 | 1.8 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 3BTF | 2000-03-13 | 1.8 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI. |
| 8PTI | 1991-04-15 | 1.8 | CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 1P2Q | 2004-04-20 | 1.8 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 1T8M | 2005-03-08 | 1.8 | CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX |
| 3U1J | 2011-11-09 | 1.8 | Aprotinin bound to Dengue virus protease |
| 1P2N | 2004-04-20 | 1.8 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 3TGI | 1998-12-23 | 1.8 | WILD-TYPE RAT ANIONIC TRYPSIN COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR (BPTI) |
| 3BTE | 2000-03-15 | 1.85 | The Crystal Structures of the Complexes Between Bovine Beta-Trypsin and Ten P1 Variants of BPTI. |
| 3BTK | 2000-03-13 | 1.85 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 1T7C | 2005-03-08 | 1.85 | CRYSTAL STRUCTURE OF THE P1 GLU BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX |
| 1EJM | 2001-03-03 | 1.85 | CRYSTAL STRUCTURE OF THE BPTI ALA16LEU MUTANT IN COMPLEX WITH BOVINE TRYPSIN |
| 2TGP | 1983-01-18 | 1.9 | THE GEOMETRY OF THE REACTIVE SITE AND OF THE PEPTIDE GROUPS IN TRYPSIN, TRYPSINOGEN AND ITS COMPLEXES WITH INHIBITORS |
| 1TPA | 1983-01-18 | 1.9 | THE GEOMETRY OF THE REACTIVE SITE AND OF THE PEPTIDE GROUPS IN TRYPSIN, TRYPSINOGEN AND ITS COMPLEXES WITH INHIBITORS |
| 3BTQ | 2000-03-15 | 1.9 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 1NAG | 1993-10-31 | 1.9 | CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A |
| 3BTT | 2000-03-13 | 1.9 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 5JB6 | 2017-04-19 | 1.9 | A simplified BPTI variant containing 23 alanines out of 58 residues |
| 3BTD | 2000-03-13 | 1.9 | The Crystal Structures of the Complexes Between the Bovine Beta-Trypsin and Ten P1 Variants of BPTI. |
| 5JB7 | 2017-04-19 | 1.9 | A simplified BPTI variant containing 24 alanines out of 58 residues |
| 2PTC | 1983-01-18 | 1.9 | THE GEOMETRY OF THE REACTIVE SITE AND OF THE PEPTIDE GROUPS IN TRYPSIN, TRYPSINOGEN AND ITS COMPLEXES WITH INHIBITORS |
| 3TPI | 1983-01-18 | 1.9 | THE GEOMETRY OF THE REACTIVE SITE AND OF THE PEPTIDE GROUPS IN TRYPSIN, TRYPSINOGEN AND ITS COMPLEXES WITH INHIBITORS |
| 3BTG | 2000-03-13 | 1.9 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 5JB4 | 2017-04-19 | 1.99 | A simplified BPTI variant containing 21 alanines out 58 of residues |
| 4BNR | 2013-09-04 | 2.0 | Extremely stable complex of crayfish trypsin with bovine trypsin inhibitor |
| 1P2O | 2004-04-20 | 2.0 | Structural consequences of accommodation of four non-cognate amino-acid residues in the S1 pocket of bovine trypsin and chymotrypsin |
| 1BPT | 1993-01-15 | 2.0 | CREVICE-FORMING MUTANTS OF BPTI: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A |
| 1FAN | 1993-10-31 | 2.0 | CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A |
| 3BTW | 2000-03-13 | 2.05 | THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI |
| 1BRB | 1994-07-31 | 2.1 | CRYSTAL STRUCTURES OF RAT ANIONIC TRYPSIN COMPLEXED WITH THE PROTEIN INHIBITORS APPI AND BPTI |
| 2HEX | 1999-08-05 | 2.1 | DECAMERS OBSERVED IN THE CRYSTALS OF BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 2TPI | 1982-03-04 | 2.1 | ON THE DISORDERED ACTIVATION DOMAIN IN TRYPSINOGEN. CHEMICAL LABELLING AND LOW-TEMPERATURE CRYSTALLOGRAPHY |
| 1FAK | 1999-12-03 | 2.1 | HUMAN TISSUE FACTOR COMPLEXED WITH COAGULATION FACTOR VIIA INHIBITED WITH A BPTI-MUTANT |
| 4WXV | 2015-07-22 | 2.1 | Human cationic trypsin K97D mutant in complex with bovine pancreatic trypsin inhibitor (BPTI) |
| 1BZX | 1998-11-11 | 2.1 | THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN COMPLEX WITH BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 1BTI | 1993-10-31 | 2.2 | CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A |
| 3LDI | 2010-06-23 | 2.2 | Crystal structure of aprotinin in complex with sucrose octasulfate: unusual interactions and implication for heparin binding |
| 4TPI | 1985-11-08 | 2.2 | THE REFINED 2.2-ANGSTROMS (0.22-NM) X-RAY CRYSTAL STRUCTURE OF THE TERNARY COMPLEX FORMED BY BOVINE TRYPSINOGEN, VALINE-VALINE AND THE ARG15 ANALOGUE OF BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 3TGJ | 1998-12-23 | 2.2 | S195A TRYPSINOGEN COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR (BPTI) |
| 1BTH | 1997-12-24 | 2.3 | STRUCTURE OF THROMBIN COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR |
| 2IJO | 2007-05-15 | 2.3 | Crystal Structure of the West Nile virus NS2B-NS3 protease complexed with bovine pancreatic trypsin inhibitor |
| 5YVU | 2018-11-28 | 2.49 | Crystal structures of unlinked full length NS3 from Dengue virus provide insights into dynamics of protease domain |
| 2KAI | 1984-07-19 | 2.5 | REFINED 2.5 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF THE COMPLEX FORMED BY PORCINE KALLIKREIN A AND THE BOVINE PANCREATIC TRYPSIN INHIBITOR. CRYSTALLIZATION, PATTERSON SEARCH, STRUCTURE DETERMINATION, REFINEMENT, STRUCTURE AND COMPARISON WITH ITS COMPONENTS AND WITH THE BOVINE TRYPSIN-PANCREATIC TRYPSIN INHIBITOR COMPLEX |
| 1BZ5 | 1998-11-11 | 2.58 | EVIDENCE OF A COMMON DECAMER IN THREE CRYSTAL STRUCTURES OF BPTI, CRYSTALLIZE FROM THIOCYANATE, CHLORIDE OR SULFATE |
| 1CBW | 1997-07-23 | 2.6 | BOVINE CHYMOTRYPSIN COMPLEXED TO BPTI |
| 5YW1 | 2019-01-02 | 2.6 | Crystal structure of full length NS3 protein (eD4NS2BNS3) in complex with Bovine Pancreatic Trypsin Inhibitor |
| 3LDM | 2010-06-23 | 2.6 | Crystal structure of aprotinin in complex with sucrose octasulfate: unusual interactions and implication for heparin binding |
| 1BHC | 1998-09-16 | 2.7 | BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE |
| 1B0C | 1998-11-11 | 2.8 | EVIDENCE OF A COMMON DECAMER IN THREE CRYSTAL STRUCTURES OF BPTI, CRYSTALLIZED FROM THIOCYANATE, CHLORIDE OR SULFATE |
| 3GYM | 2009-05-05 | 2.8 | Structure of Prostasin in Complex with Aprotinin |
| 1MTN | 1996-08-17 | 2.8 | BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION |
| 1EAW | 2002-01-28 | 2.93 | Crystal structure of the MTSP1 (matriptase)-BPTI (aprotinin) complex |