Stabilization of lambda repressor against thermal denaturation by site-directed Gly----Ala changes in alpha-helix 3.


Abstract

Oligonucleotide-directed mutagenesis has been used to replace alpha-helical glycines in the N-terminal domain of lambda repressor with alanines. Since alanine is a significantly better helix-forming residue than glycine, these changes were predicted to have a stabilizing effect. We show that the Gly46----Ala substitution, the Gly48----Ala substitution, and the double substitution increase the melting temperature of the N-terminal domain by 3-6 degrees. Study holds ProTherm entries: 790, 791, 792, 793, 2273, 2274, 13412, 13413, 13414, 13985, 13986 Extra Details: alpha helix; lambda repressor; stabilizing effect;,melting temperature

Submission Details

ID: AdDkHwEm

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:15 p.m.

Version: 1

Publication Details
Hecht MH;Sturtevant JM;Sauer RT,Proteins (1986) Stabilization of lambda repressor against thermal denaturation by site-directed Gly----Ala changes in alpha-helix 3. PMID:3449850
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1F39 2000-06-01T00:00:00+0000 1.9 CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR C-TERMINAL DOMAIN
1KCA 2001-11-07T00:00:00+0000 2.91 Crystal Structure of the lambda Repressor C-terminal Domain Octamer
1LLI 1994-03-25T00:00:00+0000 2.1 THE CRYSTAL STRUCTURE OF A MUTANT PROTEIN WITH ALTERED BUT IMPROVED HYDROPHOBIC CORE PACKING
1LMB 1991-11-05T00:00:00+0000 1.8 REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX
1LRP 1987-12-04T00:00:00+0000 3.2 COMPARISON OF THE STRUCTURES OF CRO AND LAMBDA REPRESSOR PROTEINS FROM BACTERIOPHAGE LAMBDA
1RIO 2003-11-17T00:00:00+0000 2.3 Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
3BDN 2007-11-15T00:00:00+0000 3.91 Crystal Structure of the Lambda Repressor
3KZ3 2009-12-07T00:00:00+0000 1.64 A structure of a lambda repressor fragment mutant
3WOA 2013-12-25T00:00:00+0000 2.0 Crystal structure of lambda repressor (1-45) fused with maltose-binding protein
5ZCA 2018-02-16T00:00:00+0000 1.8 Crystal structure of lambda repressor (1-20) fused with maltose-binding protein

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Repressor protein cI P03034 RPC1_LAMBD