A comprehensive, high-resolution map of a gene's fitness landscape.


Abstract

Mutations are central to evolution, providing the genetic variation upon which selection acts. A mutation's effect on the suitability of a gene to perform a particular function (gene fitness) can be positive, negative, or neutral. Knowledge of the distribution of fitness effects (DFE) of mutations is fundamental for understanding evolutionary dynamics, molecular-level genetic variation, complex genetic disease, the accumulation of deleterious mutations, and the molecular clock. We present comprehensive DFEs for point and codon mutants of the Escherichia coli TEM-1 β-lactamase gene and missense mutations in the TEM-1 protein. These DFEs provide insight into the inherent benefits of the genetic code's architecture, support for the hypothesis that mRNA stability dictates codon usage at the beginning of genes, an extensive framework for understanding protein mutational tolerance, and evidence that mutational effects on protein thermodynamic stability shape the DFE. Contrary to prevailing expectations, we find that deleterious effects of mutation primarily arise from a decrease in specific protein activity and not cellular protein levels.

Submission Details

ID: 9qCh9wU7

Submitter: Shu-Ching Ou

Submission Date: July 23, 2018, 8:51 p.m.

Version: 1

Publication Details
Firnberg E;Labonte JW;Gray JJ;Ostermeier M,Mol Biol Evol (2014) A comprehensive, high-resolution map of a gene's fitness landscape. PMID:24567513
Additional Information

Study Summary

Number of data points 10938
Proteins Beta-lactamase TEM
Unique complexes 5199
Assays/Quantities/Protocols Experimental Assay: Mutational Tolerance
Libraries Protein fitness values for ampicillin resistance

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1M40 2002-07-17 0.85 ULTRA HIGH RESOLUTION CRYSTAL STRUCTURE OF TEM-1
4ID4 2013-12-25 1.05 Crystal structure of chimeric beta-lactamase cTEM-17m
4R4S 2015-11-11 1.1 Crystal structure of chimeric beta-lactamase cTEM-19m at 1.1 angstrom resolution
4QY6 2015-08-12 1.15 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations
1NYM 2003-08-26 1.2 Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (CXB)
4R4R 2015-11-11 1.2 Crystal structure of chimeric beta-lactamase cTEM-19m at 1.2 angstrom resolution
4RX3 2015-03-04 1.39 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis
1YT4 2005-07-12 1.4 Crystal structure of TEM-76 beta-lactamase at 1.4 Angstrom resolution
5HW5 2017-06-28 1.41 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon
4RVA 2015-03-04 1.44 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for deacylation
6AYK 2018-09-12 1.44 Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon
1PZP 2004-03-09 1.45 TEM-1 Beta-Lactamase in Complex with a Novel, Core-Disrupting, Allosteric Inhibitor
5KPU 2017-08-09 1.5 Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon
4QY5 2015-08-12 1.5 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations
1LI9 2002-09-11 1.52 Crystal structure of TEM-34 beta-Lactamase at 1.5 Angstrom
4ZJ1 2015-05-20 1.54 Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli : V216AcrF mutant
1ZG4 2005-05-17 1.55 TEM1 beta lactamase
1NXY 2003-08-26 1.6 Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (SM2)
2V1Z 2008-06-24 1.6 Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions.
1LI0 2002-09-11 1.61 Crystal structure of TEM-32 beta-Lactamase at 1.6 Angstrom
5HVI 2017-06-28 1.64 Crystal structure of TEM1 beta-lactamase
2B5R 2006-04-11 1.65 1B Lactamase / B Lactamase Inhibitor
2V20 2008-06-24 1.67 Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions. Complex with sulfate.
1ERM 2000-05-10 1.7 X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXYPHENYL)ETHANE BORONIC ACID
5HW1 2017-06-28 1.7 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon
1FQG 2000-11-01 1.7 MOLECULAR STRUCTURE OF THE ACYL-ENZYME INTERMEDIATE IN TEM-1 BETA-LACTAMASE
4ZJ3 2015-05-20 1.7 Crystal structure of cephalexin bound acyl-enzyme intermediate of Val216AcrF mutant TEM1 beta-lactamase from Escherichia coli: E166N and V216AcrF mutant.
1JTG 2001-10-17 1.73 CRYSTAL STRUCTURE OF TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN COMPLEX
1JVJ 2002-03-06 1.73 CRYSTAL STRUCTURE OF N132A MUTANT OF TEM-1 BETA-LACTAMASE IN COMPLEX WITH A N-FORMIMIDOYL-THIENAMYCINE
5I52 2017-06-28 1.75 Crystal structure of TEM1 beta-lactamase mutant I263N
1NY0 2003-08-26 1.75 Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (NBF)
1JWP 2002-06-05 1.75 Structure of M182T mutant of TEM-1 beta-lactamase
1BTL 1995-01-26 1.8 CRYSTAL STRUCTURE OF ESCHERICHIA COLI TEM1 BETA-LACTAMASE AT 1.8 ANGSTROMS RESOLUTION
4ZJ2 2015-05-20 1.8 Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli :E166N mutant
1JWZ 2002-06-05 1.8 Crystal structure of TEM-64 beta-lactamase in complex with a boronic acid inhibitor (105)
1BT5 1999-09-02 1.8 CRYSTAL STRUCTURE OF THE IMIPENEM INHIBITED TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI
5KKF 2017-06-28 1.82 Crystal structure of TEM1 beta-lactamase mutant I263L
1JWV 2002-06-05 1.85 Crystal structure of G238A mutant of TEM-1 beta-lactamase in complex with a boronic acid inhibitor (sefb4)
6APA 2018-08-22 1.86 Crystal structure of TEM1 beta-lactamase mutant I263A
3TOI 2012-05-16 1.9 Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization
1NYY 2003-08-26 1.9 Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (105)
1ERQ 2000-05-10 1.9 X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID
1XPB 1997-04-01 1.9 STRUCTURE OF BETA-LACTAMASE TEM1
1XXM 2005-01-18 1.9 The modular architecture of protein-protein binding site
1PZO 2004-03-09 1.9 TEM-1 Beta-Lactamase in Complex with a Novel, Core-Disrupting, Allosteric Inhibitor
4GKU 2012-10-10 1.91 Crystal structure of beta lactamase in PET-15B
3CMZ 2008-11-25 1.92 TEM-1 Class-A beta-lactamase L201P mutant apo structure
1TEM 1997-05-15 1.95 6 ALPHA HYDROXYMETHYL PENICILLOIC ACID ACYLATED ON THE TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI
5I63 2017-06-28 1.95 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon
5NPO 2017-12-20 1.95 Promiscuous Protein Self-Assembly as a Function of Protein Stability
1LHY 2002-09-11 2.0 Crystal structure of TEM-30 beta-Lactamase at 2.0 Angstrom
1ESU 2000-05-03 2.0 S235A MUTANT OF TEM1 BETA-LACTAMASE
3DTM 2008-08-05 2.0 Increased folding stability of TEM-1 beta-lactamase by in-vitro selection
1AXB 1998-10-28 2.0 TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI INHIBITED WITH AN ACYLATION TRANSITION STATE ANALOG
6B2N 2018-01-17 2.0 Crystal structure of TEM-1 beta-lactamase mutant M182N
4MEZ 2014-10-15 2.05 Crystal structure of M68L/M69T double mutant TEM-1
5IQ8 2017-06-28 2.06 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide
3C7V 2008-10-07 2.07 Structural Insight into the Kinetics and Delta-Cp of interactions between TEM-1 Beta-Lactamase and BLIP
1ZG6 2005-05-17 2.1 TEM1 beta lactamase mutant S70G
1ERO 2000-05-10 2.1 X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID
4IBR 2013-04-03 2.2 Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S/E104K mutations
3C7U 2008-10-07 2.2 Structural Insight into the Kinetics and Cp of interactions between TEM-1-Lactamase and BLIP
1CK3 1999-08-18 2.28 N276D MUTANT OF ESCHERICHIA COLI TEM-1 BETA-LACTAMASE
4DXB 2012-08-08 2.29 2.29A structure of the engineered MBP TEM-1 fusion protein RG13 in complex with zinc, P1 space group
1JTD 2001-10-03 2.3 Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase
1S0W 2004-02-10 2.3 1b Lactamse/ b Lactamase Inhibitor
4DXC 2012-08-08 2.3 Crystal structure of the engineered MBP TEM-1 fusion protein RG13, C2 space group
4RX2 2015-03-04 2.32 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis
4IBX 2013-04-03 2.68 Crystal structure of stabilized TEM-1 beta-lactamase variant v.13
3JYI 2009-10-06 2.7 Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.7 Beta-lactamase TEM Q48406 BLAT_KLEOX
100.0 Beta-lactamase TEM P62594 BLAT_SALTI
100.0 Beta-lactamase TEM P62593 BLAT_ECOLX