Tryptophan repressor of Escherichia coli shows unusual thermal stability.

Abstract

Differential scanning calorimetry demonstrates that the tryptophan repressor of Escherichia coli is unusually resistant to thermal denaturation. The dimeric protein undergoes reversible dissociative unfolding at pH 7.5 centered at about 90 degrees C. The thermal stability may be due in part to the unusual structure of the protein, which is composed of two identical intertwined polypeptide chains. Study holds ProTherm entries: 3001 Extra Details: tryptophan repressor; thermal stability; dimeric protein;,reversible dissociative unfolding

Submission Details

ID: 9oeoSFn93

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:20 p.m.

Version: 1

Publication Details
Bae SJ;Chou WY;Matthews K;Sturtevant JM,Proc. Natl. Acad. Sci. U.S.A. (1988) Tryptophan repressor of Escherichia coli shows unusual thermal stability. PMID:3045823
Additional Information

Study Summary

Number of data points 4
Proteins Trp operon repressor ; Trp operon repressor
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dCp ; Experimental Assay: dHcal ; Experimental Assay: Tm ; Experimental Assay: dHvH
Libraries Mutations for sequence AQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEEVLLKSD

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Trp operon repressor A7ZVT5 TRPR_ECO24
100.0 Trp operon repressor B7LEN9 TRPR_ECO55
100.0 Trp operon repressor P0A882 TRPR_ECO57
100.0 Trp operon repressor B5Z4S5 TRPR_ECO5E
100.0 Trp operon repressor B7LXV6 TRPR_ECO8A
100.0 Trp operon repressor C4ZT75 TRPR_ECOBW
100.0 Trp operon repressor B1XFK3 TRPR_ECODH
100.0 Trp operon repressor A8A8C2 TRPR_ECOHS
100.0 Trp operon repressor B1IS26 TRPR_ECOLC
100.0 Trp operon repressor P0A881 TRPR_ECOLI
100.0 Trp operon repressor B6I6P1 TRPR_ECOSE
100.0 Trp operon repressor B2TZS6 TRPR_SHIB3
100.0 Trp operon repressor Q31SU5 TRPR_SHIBS
100.0 Trp operon repressor Q327K2 TRPR_SHIDS
100.0 Trp operon repressor Q0SX19 TRPR_SHIF8
100.0 Trp operon repressor P0A883 TRPR_SHIFL
100.0 Trp operon repressor Q3YU01 TRPR_SHISS
99.1 Trp operon repressor B7UR23 TRPR_ECO27
99.1 Trp operon repressor B7MNK2 TRPR_ECO45
99.1 Trp operon repressor B7N2W3 TRPR_ECO81
99.1 Trp operon repressor A1AJW2 TRPR_ECOK1
99.1 Trp operon repressor Q0T8R8 TRPR_ECOL5
99.1 Trp operon repressor Q8FA42 TRPR_ECOL6
99.1 Trp operon repressor Q1R248 TRPR_ECOUT
100.0 Trp operon repressor B7NW74 TRPR_ECO7I
100.0 Trp operon repressor B7NH68 TRPR_ECOLU
100.0 Trp operon repressor B7LNT5 TRPR_ESCF3
99.1 Trp operon repressor B1LEK0 TRPR_ECOSM
90.4 Trp operon repressor A9MR96 TRPR_SALAR