Exploring the origins of binding specificity through the computational redesign of calmodulin.


Abstract

Calmodulin (CaM) is a second messenger protein that has evolved to bind tightly to a variety of targets and, as such, exhibits low binding specificity. We redesigned CaM by using a computational protein design algorithm to improve its binding specificity for one of its targets, smooth muscle myosin light chain kinase (smMLCK). Residues in or near the CaM/smMLCK binding interface were optimized; CaM interactions with alternative targets were not directly considered in the optimization. The predicted CaM sequences were constructed and tested for binding to a set of eight targets including smMLCK. The best CaM variant, obtained from a calculation that emphasized intermolecular interactions, showed up to a 155-fold increase in binding specificity. The increase in binding specificity was not due to improved binding to smMLCK, but due to decreased binding to the alternative targets. This finding is consistent with the fact that the sequence of wild-type CaM is nearly optimal for interactions with numerous targets.

Submission Details

ID: 9njU6qMX

Submitter: Marie Ary

Submission Date: Oct. 31, 2018, 3:40 p.m.

Version: 1

Publication Details
Shifman JM;Mayo SL,Proc Natl Acad Sci U S A (2003) Exploring the origins of binding specificity through the computational redesign of calmodulin. PMID:14597710
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2N27 2016-07-06 Competitive inhibition of TRPV1 calmodulin interaction by vanilloids
2KUG 2010-03-09 Halothane binds to druggable sites in calcium-calmodulin: Solution Structure of halothane-CaM N-terminal domain
2KUH 2010-03-09 Halothane binds to druggable sites in calcium-calmodulin: Solution structure of halothane-CaM C-terminal domain
2LLO 2012-02-01 Solution NMR-derived structure of calmodulin N-lobe bound with ER alpha peptide
1CKK 1999-09-10 CALMODULIN/RAT CA2+/CALMODULIN DEPENDENT PROTEIN KINASE FRAGMENT
2KDU 2009-12-15 Structural basis of the Munc13-1/Ca2+-Calmodulin interaction: A novel 1-26 calmodulin binding motif with a bipartite binding mode
2N6A 2015-08-26 NMR structure of a human calmodulin/connexin-36 peptide hybrid
1SW8 2004-04-06 Solution structure of the N-terminal domain of Human N60D calmodulin refined with paramagnetism based strategy
6FEG 2018-02-21 Solution Structure of CaM/Kv7.2-hAB Complex
1X02 2006-03-07 Solution structure of stereo array isotope labeled (SAIL) calmodulin
2K0E 2008-06-10 A Coupled Equilibrium Shift Mechanism in Calmodulin-Mediated Signal Transduction
2HF5 2007-05-01 The structure and function of a novel two-site calcium-binding fragment of calmodulin
2LL6 2012-05-16 Solution NMR structure of CaM bound to iNOS CaM binding domain peptide
2K3S 2008-05-27 HADDOCK-derived structure of the CH-domain of the smoothelin-like 1 complexed with the C-domain of apocalmodulin
2L7L 2011-05-18 Solution structure of Ca2+/calmodulin complexed with a peptide representing the calmodulin-binding domain of calmodulin kinase I
1F71 2000-09-22 REFINED SOLUTION STRUCTURE OF CALMODULIN C-TERMINAL DOMAIN
5TP6 2017-09-27 Solution structure of the CaM34 with the iNOS CaM binding domain peptide
2N8J 2016-10-12 Structure and 15N relaxation data of Calmodulin bound to the endothelial Nitric Oxide Synthase Calmodulin Binding Domain Peptide at Physiological Calcium Concentration
6BUT 2019-06-19 Solution structure of full-length apo mammalian calmodulin bound to the IQ motif of the human voltage-gated sodium channel NaV1.2
2M0K 2013-10-30 3D Structure of Calmodulin and Calmodulin Binding Domain of Rat Olfactory Cyclic Nucleotide-Gated Ion Channel
5TP5 2017-09-27 Solution structure of the calcium deficient mutant calmodulin CaM1234
1MUX 1998-10-14 SOLUTION NMR STRUCTURE OF CALMODULIN/W-7 COMPLEX: THE BASIS OF DIVERSITY IN MOLECULAR RECOGNITION, 30 STRUCTURES
6GDL 2018-10-17 Calmodulin mutant - F141L apo-form Unstructured C-domain
2L53 2011-01-05 Solution NMR Structure of apo-calmodulin in complex with the IQ motif of Human Cardiac Sodium Channel NaV1.5
2LLQ 2012-02-01 Solution nmr-derived structure of calmodulin c-lobe bound with er alpha peptide
2KZ2 2011-04-20 Calmodulin, C-terminal domain, F92E mutant
2DFS 2006-04-25 3-D structure of Myosin-V inhibited state
2LV6 2013-02-20 The complex between Ca-Calmodulin and skeletal muscle myosin light chain kinase from combination of NMR and aqueous and contrast-matched SAXS data
1NWD 2003-04-08 Solution Structure of Ca2+/Calmodulin bound to the C-terminal Domain of Petunia Glutamate Decarboxylase
1AK8 1997-09-17 NMR SOLUTION STRUCTURE OF CERIUM-LOADED CALMODULIN AMINO-TERMINAL DOMAIN (CE2-TR1C), 23 STRUCTURES
5OEO 2018-04-25 Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant
6FEH 2018-02-21 Solution Structure of CaM/Kv7.2-hAB Complex
1CFC 1995-12-07 CALCIUM-FREE CALMODULIN
4BYA 2014-06-18 Calmodulin, C-terminal domain, M144H mutant
1F70 2000-09-22 REFINED SOLUTION STRUCTURE OF CALMODULIN N-TERMINAL DOMAIN
2LL7 2012-05-16 Solution NMR structure of CaM bound to the eNOS CaM binding domain peptide
2MES 2014-12-24 Backbone 1H, 13C, 15N resonance assignments of calcium-bound calmodulin in complex with PSD95 N-terminal peptide
1J7P 2001-11-07 Solution structure of Calcium calmodulin C-terminal domain
2KNE 2009-11-24 Calmodulin wraps around its binding domain in the plasma membrane CA2+ pump anchored by a novel 18-1 motif
1CMF 1995-12-07 NMR SOLUTION STRUCTURE OF APO CALMODULIN CARBOXY-TERMINAL DOMAIN
2LQP 2012-06-06 NMR solution structure of the Ca2+-Calmodulin C-terminal domain in a complex with a peptide (NSCaTE) from the L-type Voltage-Gated Calcium Channel alpha1C subunit
2RRT 2011-05-25 Solution structure of Magnesium-bound form of calmodulin C-domain E104D/E140D mutant
5J8H 2016-09-07 Structure of calmodulin in a complex with a peptide derived from a calmodulin-dependent kinase
2JZI 2009-01-13 Structure of Calmodulin complexed with the Calmodulin Binding Domain of Calcineurin
2M55 2013-05-08 NMR structure of the complex of an N-terminally acetylated alpha-synuclein peptide with calmodulin
1NKF 1999-02-16 CALCIUM-BINDING PEPTIDE, NMR, 30 STRUCTURES
1SY9 2005-04-12 Structure of calmodulin complexed with a fragment of the olfactory CNG channel
2K0F 2008-06-10 Calmodulin complexed with calmodulin-binding peptide from smooth muscle myosin light chain kinase
2M0J 2013-10-30 3D Structure of Calmodulin and Calmodulin binding domain of Olfactory cyclic nucleotide-gated ion channel complex
5GGM 2016-09-28 The NMR structure of calmodulin in CTAB reverse micelles
1CFF 1999-09-24 NMR SOLUTION STRUCTURE OF A COMPLEX OF CALMODULIN WITH A BINDING PEPTIDE OF THE CA2+-PUMP
2MG5 2014-03-05 Solution Structure of Calmodulin bound to the target peptide of Endothelial Nitrogen Oxide Synthase phosphorylated at Thr495
2LGF 2012-06-13 Solution structure of Ca2+/calmodulin complexed with a peptide representing the calmodulin-binding domain of L-selectin
1CFD 1995-12-07 CALCIUM-FREE CALMODULIN
2K61 2009-05-05 Solution structure of CaM complexed to DAPk peptide
2MGU 2014-02-12 Structure of the complex between calmodulin and the binding domain of HIV-1 matrix protein
5T0X 2017-02-15 Solution NMR-derived structure of calmodulin bound with ER alpha peptides
1CMG 1995-12-07 NMR SOLUTION STRUCTURE OF CALCIUM-LOADED CALMODULIN CARBOXY-TERMINAL DOMAIN
2LQC 2012-06-06 NMR solution structure of a Ca2+-Calmodulin with a binding motif (NSCaTE) peptide from the N-terminal cytoplasmic domain of the L-type Voltage-Cated Calcium Channel alpha1C subunit
6GDK 2018-10-17 Calcium bound form of human calmodulin mutant F141L
2K0J 2009-03-10 Solution structure of CaM complexed to DRP1p
2N77 2016-11-30 NMR solution structure of a complex of PEP-19 bound to the C-domain of apo calmodulin
5J7J 2017-10-25 NMR Derived Structure of Ca2+ Calmodulin bound to Phosphorylated PSD-95
1J7O 2001-11-07 Solution structure of Calcium-calmodulin N-terminal domain
2M3S 2013-07-24 Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant
1DMO 1996-08-01 CALMODULIN, NMR, 30 STRUCTURES
2O5G 2007-12-25 1.08 Calmodulin-smooth muscle light chain kinase peptide complex
2PQ3 2007-10-16 1.3 N-Terminal Calmodulin Zn-Trapped Intermediate
4DJC 2012-02-22 1.35 1.35 A crystal structure of the NaV1.5 DIII-IV-Ca/CaM complex
2W73 2009-05-12 1.45 High-resolution structure of the complex between calmodulin and a peptide from calcineurin A
3GOF 2010-03-31 1.45 Calmodulin bound to peptide from macrophage nitric oxide synthase
2F3Y 2005-12-27 1.45 Calmodulin/IQ domain complex
3GP2 2010-04-07 1.46 Calmodulin bound to peptide from calmodulin kinase II (CaMKII)
4LZX 2014-05-07 1.5 Complex of IQCG and Ca2+-free CaM
4J9Y 2013-03-27 1.51 Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant
2O60 2007-12-25 1.55 Calmodulin bound to peptide from neuronal nitric oxide synthase
5V03 2018-03-07 1.58 A positive allosteric modulator binding pocket in SK2 ion channels is shared by Riluzole and CyPPA
2F3Z 2005-12-27 1.6 Calmodulin/IQ-AA domain complex
3IF7 2010-06-30 1.6 Structure of Calmodulin complexed with its first endogenous inhibitor, sphingosylphosphorylcholine
3B32 2007-11-13 1.6 Crystal Structure of Calcium-Saturated Calmodulin N-Terminal Domain Fragment, Residues 1-75
1G4Y 2001-05-09 1.6 1.60 A CRYSTAL STRUCTURE OF THE GATING DOMAIN FROM SMALL CONDUCTANCE POTASSIUM CHANNEL COMPLEXED WITH CALCIUM-CALMODULIN
4G28 2012-09-12 1.63 Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant and EBIO-1
6DAD 2018-10-17 1.65 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex
4G27 2012-09-12 1.65 Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant and phenylurea
4J9Z 2013-03-27 1.66 Calcium-calmodulin complexed with the calmodulin binding domain from a small conductance potassium channel splice variant and NS309
1FW4 2001-05-02 1.7 CRYSTAL STRUCTURE OF E. COLI FRAGMENT TR2C FROM CALMODULIN TO 1.7 A RESOLUTION
5NIN 2017-12-06 1.7 Crystal Structure of AKAP79 calmodulin binding domain peptide in complex with Ca2+/Calmodulin
1YR5 2006-07-04 1.7 1.7-A structure of calmodulin bound to a peptide from DAP kinase
5DOW 2016-08-10 1.7 Solution of the Variably-Twinned Structure of a Novel Calmodulin-Peptide Complex in a Novel Configuration
3SG6 2012-06-20 1.7 Crystal Structure of Dimeric GCaMP2-LIA(linker 1)
1CLL 1993-10-31 1.7 CALMODULIN STRUCTURE REFINED AT 1.7 ANGSTROMS RESOLUTION
1PRW 2003-10-10 1.7 Crystal structure of bovine brain Ca++ calmodulin in a compact form
5K8Q 2016-08-24 1.74 Crystal Structure of Calcium-loaded Calmodulin in complex with STRA6 CaMBP2-site peptide.
3EVU 2008-12-09 1.75 Crystal structure of Calcium bound dimeric GCAMP2
3UCW 2012-08-08 1.76 Structure of MG2+ bound N-Terminal domain of Calmodulin
5V02 2018-03-07 1.78 A positive allosteric modulator binding pocket in SK2 ion channels is shared by Riluzole and CyPPA
3UCY 2012-08-08 1.8 Structure of Mg2+ bound N-terminal domain of calmodulin in the presence of Zn2+
5JQA 2017-10-11 1.8 CaM:RM20 complex
1AHR 1997-06-16 1.8 CALMODULIN MUTANT WITH A TWO RESIDUE DELETION IN THE CENTRAL HELIX
2Y4V 2011-12-28 1.8 CRYSTAL STRUCTURE OF HUMAN CALMODULIN IN COMPLEX WITH A DAP KINASE-1 MUTANT (W305Y) PEPTIDE
1IQ5 2001-09-26 1.8 Calmodulin/nematode CA2+/Calmodulin dependent kinase kinase fragment
4BW8 2014-01-15 1.8 Calmodulin with small bend in central helix
4Q57 2014-07-23 1.8 Crystal structure of the plectin 1a actin-binding domain/N-terminal domain of calmodulin complex
6MBA 2019-04-10 1.8 Crystal Structure of Human Nav1.4 CTerminal Domain in Complex with apo Calmodulin
4BW7 2014-01-15 1.81 Calmodulin in complex with strontium
5JTH 2017-07-19 1.84 Crystal structure of E67A calmodulin - CaM:RM20 complex
3BYA 2009-01-20 1.85 Structure of a Calmodulin Complex
3EK7 2008-12-16 1.85 Calcium-saturated GCaMP2 dimer
2R28 2008-07-01 1.86 The complex Structure of Calmodulin Bound to a Calcineurin Peptide
6O5G 2019-08-07 1.89 Calmodulin in complex with isomalbrancheamide D
5WBX 2017-12-20 1.9 Structural insights into the potency of SK/IK channel positive modulators
2WEL 2009-04-14 1.9 Crystal structure of SU6656-bound calcium/calmodulin-dependent protein kinase II delta in complex with calmodulin
6MUE 2019-05-08 1.9 Voltage-gated sodium channel NaV1.4 IQ domain in complex with Ca2+/Calmodulin
1UP5 2005-03-24 1.9 Chicken Calmodulin
3SG7 2012-06-20 1.9 Crystal Structure of GCaMP3-KF(linker 1)
4EHQ 2012-11-07 1.9 Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode
3UCT 2012-08-08 1.9 Structure of Mn2+-bound N-terminal domain of calmodulin in the presence of Zn2+
3SJQ 2012-05-30 1.9 Crystal structure of a small conductance potassium channel splice variant complexed with calcium-calmodulin
3SG5 2012-06-20 1.9 Crystal Structure of Dimeric GCaMP3-D380Y, QP(linker 1), LP(linker 2)
2HQW 2007-11-13 1.9 Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide
1ZUZ 2006-11-14 1.91 Calmodulin in complex with a mutant peptide from human DRP-1 kinase
5DSU 2017-01-11 1.93 Crystal structure of double mutant of N-domain of human calmodulin
2VAY 2008-09-16 1.94 Calmodulin complexed with CaV1.1 IQ peptide
4Q5U 2014-09-03 1.95 Structure of calmodulin bound to its recognition site from calcineurin
3SUI 2012-09-12 1.95 Crystal structure of ca2+-calmodulin in complex with a trpv1 c-terminal peptide
3WFN 2013-08-28 1.95 Crystal Structure of Nav1.6 IQ motif in complex with apo-CaM
6EEB 2019-08-07 1.96 Calmodulin in complex with malbrancheamide
1LIN 1996-03-08 2.0 CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:4 COMPLEX)
2BE6 2005-11-15 2.0 2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex
3EKH 2008-12-16 2.0 Calcium-saturated GCaMP2 T116V/K378W mutant monomer
2BCX 2006-10-31 2.0 Crystal structure of calmodulin in complex with a ryanodine receptor peptide
3SG2 2012-06-20 2.0 Crystal Structure of GCaMP2-T116V,D381Y
4RJD 2015-08-26 2.0 TFP bound in alternate orientations to calcium-saturated Calmodulin C-Domains
6HCS 2018-12-05 2.0 Crystal structure of CaM-peptide complex containing AzF at position 108
5J03 2016-09-07 2.0 Crystal Structure of a chimeric Kv7.2 - Kv7.3 proximal C-terminal Domain in Complex with Calmodulin
1CM4 1998-03-04 2.0 Motions of calmodulin-four-conformer refinement
1IWQ 2003-03-11 2.0 Crystal Structure of MARCKS calmodulin binding domain peptide complexed with Ca2+/Calmodulin
1CDM 1994-08-31 2.0 MODULATION OF CALMODULIN PLASTICITY IN MOLECULAR RECOGNITION ON THE BASIS OF X-RAY STRUCTURES
3EVR 2008-12-09 2.0 Crystal structure of Calcium bound monomeric GCAMP2
4HEX 2013-03-06 2.0 A novel conformation of calmodulin
1WRZ 2006-03-21 2.0 Calmodulin complexed with a peptide from a human death-associated protein kinase
1CM1 1998-03-04 2.0 MOTIONS OF CALMODULIN-SINGLE-CONFORMER REFINEMENT
2I08 2007-08-21 2.0 Solvation effect in conformational changes of EF-hand proteins: X-ray structure of Ca2+-saturated double mutant Q41L-K75I of N-domain of calmodulin
6DAE 2018-10-17 2.0 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex
1CDL 1994-08-31 2.0 TARGET ENZYME RECOGNITION BY CALMODULIN: 2.4 ANGSTROMS STRUCTURE OF A CALMODULIN-PEPTIDE COMPLEX
4QNH 2014-08-06 2.02 Calcium-calmodulin (T79D) complexed with the calmodulin binding domain from a small conductance potassium channel SK2-a
4OY4 2015-02-18 2.03 calcium-free CaMPARI v0.2
3IFK 2009-12-15 2.03 Crystal Structure Of Calcium-Saturated Calmodulin N-terminal Domain Fragment, Residues 1-90
1NIW 2003-02-18 2.05 Crystal structure of endothelial nitric oxide synthase peptide bound to calmodulin
1YRT 2006-01-17 2.1 Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin
4M1L 2014-05-07 2.1 Complex of IQCG and Ca2+-bound CaM
3SG3 2012-06-20 2.1 Crystal Structure of GCaMP3-D380Y
3G43 2009-03-03 2.1 Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer
1XA5 2004-12-21 2.12 Structure of Calmodulin in complex with KAR-2, a bis-indol alkaloid
2V01 2007-08-14 2.15 Recombinant vertebrate calmodulin complexed with Pb
6N5W 2019-03-13 2.15 Crystal structure of the Ca2+/CaM complex with independent peptides of Kv7.4 (KCNQ4) A & B domains
2F2O 2006-02-21 2.17 Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode
6B8P 2018-03-14 2.2 Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex
4I2Y 2013-07-24 2.2 Crystal Structure of the genetically encoded calcium indicator RGECO1
6CZQ 2018-08-01 2.2 A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
1ZOT 2005-08-09 2.2 crystal structure analysis of the CyaA/C-Cam with PMEAPP
4DCK 2012-06-27 2.2 Crystal structure of the C-terminus of voltage-gated sodium channel in complex with FGF13 and CaM
2V02 2007-08-14 2.2 Recombinant vertebrate calmodulin complexed with Ba
6B8N 2018-03-14 2.2 Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak
2X0G 2010-01-26 2.2 X-RAY STRUCTURE OF A DAP-KINASE CALMODULIN COMPLEX
2COL 2006-01-24 2.2 Crystal structure analysis of CyaA/C-Cam with Pyrophosphate
3CLN 1988-07-16 2.2 STRUCTURE OF CALMODULIN REFINED AT 2.2 ANGSTROMS RESOLUTION
2YGG 2011-09-28 2.23 Complex of CaMBR and CaM
5DBR 2017-02-22 2.25 Ca2+ CaM with human cardiac Na+ channel (NaV1.5) inactivation gate
1QIW 2000-03-28 2.3 Calmodulin complexed with N-(3,3,-diphenylpropyl)-N'-[1-R-(3,4-bis-butoxyphenyl)-ethyl]-propylenediamine (DPD)
3BXL 2008-03-25 2.3 Crystal structure of the R-type calcium channeL (CaV2.3) IQ domain and CA2+calmodulin complex
4L79 2014-01-29 2.3 Crystal Structure of nucleotide-free Myosin 1b residues 1-728 with bound Calmodulin
2VB6 2007-12-11 2.3 Myosin VI (MD-insert2-CaM, Delta Insert1) Post-rigor state (crystal form 2)
5WC5 2017-12-20 2.3 Structural insights into the potency of SK/IK channel positive modulators
6B8L 2018-03-14 2.3 Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex
6B8M 2018-03-14 2.3 Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak
3DVK 2008-11-04 2.3 Crystal Structure of Ca2+/CaM-CaV2.3 IQ domain complex
1L7Z 2003-09-16 2.3 Crystal structure of Ca2+/Calmodulin complexed with myristoylated CAP-23/NAP-22 peptide
5WSV 2017-06-07 2.33 Crystal structure of Myosin VIIa IQ5 in complex with Ca2+-CaM
4UPU 2014-08-20 2.34 Crystal structure of IP3 3-K calmodulin binding region in complex with Calmodulin
3DVE 2008-11-04 2.35 Crystal Structure of Ca2+/CaM-CaV2.2 IQ domain complex
3O77 2010-09-29 2.35 The structure of Ca2+ Sensor (Case-16)
1Y6W 2005-03-01 2.4 Trapped intermediate of calmodulin
3SG4 2012-06-20 2.4 Crystal Structure of GCaMP3-D380Y, LP(linker 2)
6DAF 2018-10-17 2.4 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex
3EWT 2009-10-20 2.4 Crystal Structure of calmodulin complexed with a peptide
1CTR 1994-12-20 2.45 DRUG BINDING BY CALMODULIN: CRYSTAL STRUCTURE OF A CALMODULIN-TRIFLUOPERAZINE COMPLEX
2FOT 2006-09-05 2.45 Crystal structure of the complex between calmodulin and alphaII-spectrin
3WLC 2014-01-22 2.49 Crystal structure of dimeric GCaMP6m
6DAH 2018-10-17 2.5 2.5 Angstrom crystal structure of the N97S CaM mutant
1YRU 2005-09-27 2.5 Crystal Structure analysis of the adenylyl cyclaes catalytic domain of adenylyl cyclase toxin of Bordetella pertussis in presence of c-terminal calmodulin and 1mM calcium chloride
3HR4 2009-09-08 2.5 Human iNOS Reductase and Calmodulin Complex
4ZLK 2016-05-04 2.5 Crystal structure of mouse myosin-5a in complex with calcium-bound calmodulin
2IX7 2006-12-13 2.5 Structure of apo-calmodulin bound to unconventional myosin V
6ALE 2018-08-08 2.5 A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
1QX5 2004-08-31 2.54 Crystal structure of apoCalmodulin
3OXQ 2010-11-03 2.55 Crystal Structure of Ca2+/CaM-CaV1.2 pre-IQ/IQ domain complex
3BXK 2008-03-25 2.55 Crystal structure of the P/Q-type calcium channel (CaV2.1) IQ domain and CA2+calmodulin complex
3EVV 2008-12-09 2.6 Crystal Structure of Calcium bound dimeric GCAMP2 (#2)
2F2P 2006-02-21 2.6 Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode
6B8Q 2018-03-14 2.6 Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex
3O78 2010-09-29 2.6 The structure of Ca2+ Sensor (Case-12)
4GOW 2012-12-12 2.6 Crystal Structure of Ca2+/CaM:Kv7.4 (KCNQ4) B helix complex
3EWV 2009-10-20 2.6 Crystal Structure of calmodulin complexed with a peptide
3DVM 2008-11-04 2.6 Crystal Structure of Ca2+/CaM-CaV2.1 IQ domain complex
1QIV 2000-03-28 2.64 CALMODULIN COMPLEXED WITH N-(3,3,-DIPHENYLPROPYL)-N'-[1-R-(3,4-BIS-BUTOXYPHENYL)-ETHYL]-PROPYLENEDIAMINE (DPD), 1:2 COMPLEX
3EK4 2008-12-16 2.65 Calcium-saturated GCaMP2 Monomer
5COC 2016-03-30 2.67 Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus
6MUD 2019-05-08 2.69 Voltage-gated sodium channel NaV1.5 C-terminal domain in complex with Ca2+/Calmodulin
4E53 2013-03-20 2.69 Calmodulin and Nm peptide complex
3WLD 2014-01-22 2.7 Crystal structure of monomeric GCaMP6m
4E50 2013-03-20 2.7 Calmodulin and Ng peptide complex
6K4K 2019-07-24 2.71 Crystal structure of SidJ-CaM binary complex at 2.71 A
4BYF 2014-03-26 2.74 Crystal structure of human Myosin 1c in complex with calmodulin in the pre-power stroke state
1A29 1998-09-16 2.74 CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:2 COMPLEX)
1K90 2002-01-23 2.75 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP
3EKJ 2008-12-16 2.8 Calcium-free GCaMP2 (calcium binding deficient mutant)
4OVN 2014-12-03 2.8 Voltage-gated Sodium Channel 1.5 (Nav1.5) C-terminal domain in complex with Calmodulin poised for activation
3DVJ 2008-11-04 2.8 Crystal Structure of Ca2+/CaM-CaV2.2 IQ domain (without cloning artifact, HM to TV) complex
3EK8 2008-12-16 2.8 Calcium-saturated GCaMP2 T116V/G87R mutant monomer
5HIT 2016-09-14 2.85 Crystal Structure Analysis of Ca2+-calmodulin and a C-terminal EAG1 channel fragment
4V0C 2014-11-05 2.86 Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
2BKI 2005-06-07 2.9 Myosin VI nucleotide-free (MDinsert2-IQ) crystal structure
1DEG 1994-05-31 2.9 THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE
6K4L 2019-07-24 2.95 Crystal structure of Se-labelled SidJ complex with CaM at 2.95 A
1K93 2002-01-23 2.95 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin
3J41 2013-07-31 25.0 Pseudo-atomic model of the Aquaporin-0/Calmodulin complex derived from electron microscopy
5WSU 2017-06-07 3.0 Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM
1S26 2004-04-13 3.0 Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine 5'-triphosphate Complex Reveals an Alternative Mode of ATP Binding to the Catalytic Site
4IL1 2013-10-02 3.0 Crystal Structure of the Rat Calcineurin
4UMO 2014-11-05 3.0 Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
4JPZ 2014-04-16 3.02 Voltage-gated sodium channel 1.2 C-terminal domain in complex with FGF13U and Ca2+/calmodulin
1QX7 2004-08-31 3.09 Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel
6K4R 2019-07-24 3.11 Crystal structure of SidJ-CaM-AMP ternary complex at 3.11 A
5V7X 2018-02-28 3.14 Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin
5I0I 2016-09-07 3.15 Crystal structure of myosin X motor domain with 2IQ motifs in pre-powerstroke state
1SK6 2004-06-08 3.2 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate
6C1D 2018-01-31 3.2 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
1XFX 2005-05-03 3.2 Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 10 millimolar exogenously added calcium chloride
1XFZ 2005-05-03 3.25 Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 1 millimolar exogenously added calcium chloride
1PK0 2004-02-10 3.3 Crystal Structure of the EF3-CaM complexed with PMEApp
1XFY 2005-05-03 3.3 Crystal structure of anthrax edema factor (EF) in complex with calmodulin
6O20 2019-04-24 3.3 Cryo-EM structure of TRPV5 with calmodulin bound
6MC9 2019-04-10 3.3 Crystal Structure of Human Nav1.4 C-Terminal (1599-1754) domain in complex with calcium-bound calmodulin
1XFV 2005-05-03 3.35 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP
1XFU 2005-05-03 3.35 Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin
6CNM 2018-05-02 3.4 Cryo-EM structure of the human SK4/calmodulin channel complex
1XFW 2005-05-03 3.4 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3'5' cyclic AMP (cAMP)
6CNN 2018-05-02 3.5 Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state I
4R8G 2014-12-03 3.5 Crystal Structure of Myosin-1c tail in complex with Calmodulin
1Y0V 2005-05-03 3.6 Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate
1LVC 2002-12-04 3.6 Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 2' deoxy, 3' anthraniloyl ATP
6E2G 2018-08-22 3.6 Cryo-EM structure of rat TRPV6 in complex with Calmodulin
5VMS 2017-06-07 3.7 CryoEM structure of Xenopus KCNQ1 channel
5K7L 2016-08-17 3.78 Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 bound to the channel inhibitor calmodulin
6C1G 2018-01-31 3.8 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
4JQ0 2014-05-21 3.84 Voltage-gated sodium channel 1.5 C-terminal domain in complex with FGF12B and Ca2+/calmodulin
6C1H 2018-01-31 3.9 High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing
6E2F 2018-08-22 3.9 Cryo-EM structure of human TRPV6 in complex with Calmodulin
6S5T 2019-07-24 4.15 Legionella pneumophila SidJ-Human calmodulin complex
6DMW 2018-10-24 4.4 Calmodulin-bound full-length rbTRPV5
6BNV 2018-01-10 4.6 CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine
6CNO 2018-05-02 4.7 Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state II

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
93.5 Calmodulin-1 P18061 CALM_TRYCR
93.5 Calmodulin-1 P69098 CALM_TRYBG
93.5 Calmodulin-1 P69097 CALM_TRYBB
100.0 Calmodulin-1 P62184 CALM_RENRE
100.0 Calmodulin-1 P11121 CALM_PYUSP
100.0 Calmodulin-1 P02595 CALM_PATSP
94.2 Calmodulin-1 P11118 CALM_EUGGR
100.0 Calmodulin-1 P02594 CALM_ELEEL
96.5 Calmodulin-1 Q9HFY6 CALM_BLAEM
100.0 Calmodulin-1 P05932 CALMB_ARBPU
91.2 Calmodulin-1 P0DP35 CAM2B_XENLA
91.2 Calmodulin-1 P0DP34 CAM2A_XENLA
91.2 Calmodulin-1 P62151 CALM_TETCF
91.2 Calmodulin-1 P21251 CALM_STIJA
91.2 Calmodulin-1 Q6YNX6 CALM_SHEEP
91.2 Calmodulin-1 P62160 CALM_RABIT
91.2 Calmodulin-1 Q5RAD2 CALM_PONAB
91.2 Calmodulin-1 Q71UH6 CALM_PERFV
91.2 Calmodulin-1 P62156 CALM_ONCSP
91.2 Calmodulin-1 Q6PI52 CALM_DANRE
91.2 Calmodulin-1 Q6IT78 CALM_CTEID
91.2 Calmodulin-1 P62149 CALM_CHICK
91.2 Calmodulin-1 P62157 CALM_BOVIN
91.2 Calmodulin-1 P62144 CALM_ANAPL
91.2 Calmodulin-1 P0DP31 CALM3_RAT
91.2 Calmodulin-1 P0DP28 CALM3_MOUSE
91.2 Calmodulin-1 P0DP25 CALM3_HUMAN
91.2 Calmodulin-1 P0DP30 CALM2_RAT
91.2 Calmodulin-1 P0DP27 CALM2_MOUSE
91.2 Calmodulin-1 P0DP24 CALM2_HUMAN
91.2 Calmodulin-1 Q9UB37 CALM2_BRALA
91.2 Calmodulin-1 P0DP33 CALM1_XENLA
91.2 Calmodulin-1 P0DP29 CALM1_RAT
91.2 Calmodulin-1 P0DP26 CALM1_MOUSE
91.2 Calmodulin-1 P0DP23 CALM1_HUMAN
92.9 Calmodulin-1 P41041 CALM_PNECA
97.0 Calmodulin-1 Q95NI4 CALM_HALOK
94.2 Calmodulin-1 P24044 CALM_PLAFA
94.2 Calmodulin-1 P62203 CALM_PLAF7
98.5 Calmodulin-1 O97341 CALM_SUBDO
100.0 Calmodulin-1 O96081 CALMB_HALRO
98.5 Calmodulin-1 P62150 CALM_ORYLA
93.2 Calmodulin-1 Q9GRJ1 CALM_LUMRU
96.5 Calmodulin-1 P62146 CALMA_ARBPU
90.5 Calmodulin-1 Q6R520 CALM_OREMO