Thermodynamics of unfolding of the all beta-sheet protein interleukin-1 beta.


Abstract

The thermal denaturation of interleukin-1 beta in solution has been studied by differential scanning calorimetry at various pH values. It is shown that the thermal transition of interleukin-1 beta is completely reversible below pH 2.5, only partly reversible in the pH range 2.5-3.5, and irreversible above pH 3.5. Analysis of the reversible unfolding of interleukin-1 beta shows that the heat denaturation is well approximated by a two-state transition and is accompanied by a significant increase of heat capacity. The partial heat capacity of denatured interleukin-1 beta is very close to that expected for the completely unfolded protein. This permitted us to assign the thermodynamic characteristics of interleukin-1 beta denaturation to its complete unfolding and to correlate them with structural features of the protein. The contributions of hydrogen bonding and hydrophobic interactions to the stability of interleukin-1 beta are analyzed and compared to those for other globular proteins. It is shown that the Gibbs energy of a hydrogen bond in a beta-sheet structure is greater than in alpha-helices. Study holds ProTherm entries: 4510, 4511, 4512, 4513, 4514 Extra Details:

Submission Details

ID: 9ANfwVXC

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:25 p.m.

Version: 1

Publication Details
Makhatadze GI;Clore GM;Gronenborn AM;Privalov PL,Biochemistry (1994) Thermodynamics of unfolding of the all beta-sheet protein interleukin-1 beta. PMID:8049234
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
7I1B 1992-10-15 HIGH-RESOLUTION THREE-DIMENSIONAL STRUCTURE OF INTERLEUKIN-1 BETA IN SOLUTION BY THREE-AND FOUR-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
6I1B 1992-10-15 HIGH-RESOLUTION THREE-DIMENSIONAL STRUCTURE OF INTERLEUKIN-1 BETA IN SOLUTION BY THREE-AND FOUR-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
2KH2 2009-09-08 Solution structure of a scFv-IL-1B complex
5R8Q 2020-04-22 1.23 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z2643472210 in complex with INTERLEUKIN-1 BETA
5R7W 2020-04-22 1.27 PanDDA analysis group deposition of ground-state model of INTERLEUKIN-1 BETA
5R8E 2020-04-22 1.35 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z57475877 in complex with INTERLEUKIN-1 BETA
5R8M 2020-04-22 1.39 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1818332938 in complex with INTERLEUKIN-1 BETA
5R8F 2020-04-22 1.41 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z2377835233 in complex with INTERLEUKIN-1 BETA
5R8O 2020-04-22 1.42 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1881545321 in complex with INTERLEUKIN-1 BETA
5R8G 2020-04-22 1.43 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1259086950 in complex with INTERLEUKIN-1 BETA
5R85 2020-04-22 1.44 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1262246195 in complex with INTERLEUKIN-1 BETA
6Y8I 2020-06-17 1.46 Fragment KCL_I013 in complex with IL-1-beta
5R8D 2020-04-22 1.47 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1745658474 in complex with INTERLEUKIN-1 BETA
5R8A 2020-04-22 1.47 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1492796719 in complex with INTERLEUKIN-1 BETA
5R8K 2020-04-22 1.47 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z355728146 in complex with INTERLEUKIN-1 BETA
5R87 2020-04-22 1.47 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z44592329 in complex with INTERLEUKIN-1 BETA
5R8I 2020-04-22 1.47 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z210803634 in complex with INTERLEUKIN-1 BETA
5R88 2020-04-22 1.48 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1545313172 in complex with INTERLEUKIN-1 BETA
5R8N 2020-04-22 1.48 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z57292400 in complex with INTERLEUKIN-1 BETA
4GAI 2013-02-20 1.49 Crystal structure of EBI-005, a chimera of human IL-1beta and IL-1Ra
5R8B 2020-04-22 1.49 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z2027049478 in complex with INTERLEUKIN-1 BETA
5R86 2020-04-22 1.5 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z943693514 in complex with INTERLEUKIN-1 BETA
5R8H 2020-04-22 1.5 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z111716368 in complex with INTERLEUKIN-1 BETA
2NVH 2006-12-12 1.53 Determination of Solvent Content in Cavities in Interleukin-1 Using Experimentally-Phased Electron Density
5R8P 2020-04-22 1.53 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z818727262 in complex with INTERLEUKIN-1 BETA
1L2H 2003-02-04 1.54 Crystal structure of Interleukin 1-beta F42W/W120F mutant
5R8C 2020-04-22 1.54 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z30857828 in complex with INTERLEUKIN-1 BETA
5R8L 2020-04-22 1.56 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z1891773393 in complex with INTERLEUKIN-1 BETA
5R8J 2020-04-22 1.62 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z355728146 in complex with INTERLEUKIN-1 BETA
5R89 2020-04-22 1.65 PanDDA analysis group deposition INTERLEUKIN-1 BETA -- Fragment Z217038356 in complex with INTERLEUKIN-1 BETA
3POK 2011-01-19 1.7 Interleukin-1-beta LBT L3 Mutant
4G6M 2012-12-19 1.81 Crystal structure of human IL-1beta in complex with therapeutic antibody binding fragment of gevokizumab
6Y8M 2020-03-18 1.9 Fragment bikinin bound to Interleukin 1 beta
4I1B 1990-04-15 2.0 FUNCTIONAL IMPLICATIONS OF INTERLEUKIN-1BETA BASED ON THE THREE-DIMENSIONAL STRUCTURE
1I1B 1990-01-15 2.0 CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-1BETA AT 2.0 ANGSTROMS RESOLUTION
2I1B 1990-04-15 2.0 CRYSTALLOGRAPHIC REFINEMENT OF INTERLEUKIN-1 BETA AT 2.0 ANGSTROMS RESOLUTION
1IOB 1996-08-17 2.0 INTERLEUKIN-1 BETA FROM JOINT X-RAY AND NMR REFINEMENT
4G6J 2012-12-19 2.03 Crystal structure of human IL-1beta in complex with the therapeutic antibody binding fragment of canakinumab
1T4Q 2004-12-07 2.1 Interleukin 1 beta F101W
1TOO 2004-12-07 2.1 Interleukin 1B Mutant F146W
1TWE 2004-12-07 2.1 INTERLEUKIN 1 BETA MUTANT F101Y
5I1B 1992-01-15 2.1 A COMPARISON OF THE HIGH RESOLUTION STRUCTURES OF HUMAN AND MURINE INTERLEUKIN-1B
3LTQ 2011-02-16 2.1 Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag
4GAF 2013-02-20 2.15 Crystal structure of EBI-005, a chimera of human IL-1beta and IL-1Ra, bound to human Interleukin-1 receptor type 1
5MVZ 2017-02-15 2.15 Fab 4AB007 bound to Interleukin-1-beta
1TP0 2005-02-08 2.2 Triple mutation in interleukin 1 beta cavity:replacement of phenylalanines with tryptophan.
5BVP 2015-09-02 2.2 The molecular mode of action and species specificity of canakinumab, a human monoclonal antibody neutralizing IL-1beta
1TWM 2004-12-07 2.26 Interleukin-1 Beta Mutant F146Y
9ILB 1999-01-06 2.28 HUMAN INTERLEUKIN-1 BETA
1S0L 2004-03-30 2.34 Interleukin 1 beta mutant F42W
1HIB 1994-01-31 2.4 THE STRUCTURE OF AN INTERLEUKIN-1 BETA MUTANT WITH REDUCED BIOACTIVITY SHOWS MULTIPLE SUBTLE CHANGES IN CONFORMATION THAT AFFECT PROTEIN-PROTEIN RECOGNITION
1ITB 1998-02-04 2.5 TYPE-1 INTERLEUKIN-1 RECEPTOR COMPLEXED WITH INTERLEUKIN-1 BETA
4DEP 2012-03-21 3.1 Structure of the IL-1b signaling complex
3O4O 2010-09-01 3.3 Crystal structure of an Interleukin-1 receptor complex

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
94.8 Interleukin-1 beta P46648 IL1B_CERAT
95.4 Interleukin-1 beta P79182 IL1B_MACFA
95.4 Interleukin-1 beta P51493 IL1B_MACNE
96.1 Interleukin-1 beta P48090 IL1B_MACMU
100.0 Interleukin-1 beta P01584 IL1B_HUMAN