Evidence of stable monomeric species in the unfolding of Cu,Zn superoxide dismutase from Photobacterium leiognathi.


Abstract

The equilibrium unfolding process of Photobacterium leiognathi Cu,Zn superoxide dismutase has been quantitatively monitored through circular dichroism (CD) and fluorescence spectroscopy, upon increasing the guanidinium hydrochloride concentration. The study has been undertaken for both the holo- and the copper-free derivative to work out the role of copper in protein stability. In both cases the unfolding was reversible. The denaturation curve derived from CD and fluorescence spectroscopy was not coincident, suggesting that the denaturation process occurs through a three-state model with formation of an intermediate monomeric species. The occurrence of an intermediate species has been unambiguously demonstrated following CD and steady-state fluorescence spectra of the enzyme at various concentrations in presence of a fixed amounts of guanidinium hydrochloride. Study holds ProTherm entries: 12864, 12865, 12866, 12867 Extra Details: Holo. dimer <- -> monomer(Intermediate). Transition 1. protein stability; dimeric interaction; protein unfolding; lifetime distributions

Submission Details

ID: 7PeKqsrp3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:44 p.m.

Version: 1

Publication Details
Malvezzi-Campeggi F;Stroppolo ME;Mei G;Rosato N;Desideri A,Arch. Biochem. Biophys. (1999) Evidence of stable monomeric species in the unfolding of Cu,Zn superoxide dismutase from Photobacterium leiognathi. PMID:10510278
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1OAL 2003-02-27 1.5 Active site copper and zinc ions modulate the quaternary structure of prokaryotic Cu,Zn superoxide dismutase
1OAJ 2003-02-27 1.73 Active site copper and zinc ions modulate the quaternary structure of prokaryotic Cu,Zn superoxide dismutase
1YAI 1997-08-20 1.9 X-RAY STRUCTURE OF A BACTERIAL COPPER,ZINC SUPEROXIDE DISMUTASE
1IBD 2001-05-09 2.0 X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT V29A
1IBH 2001-05-09 2.0 X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT M41I
1BZO 1999-04-09 2.1 THREE-DIMENSIONAL STRUCTURE OF PROKARYOTIC CU,ZN SUPEROXIDE DISMUTASE FROM P.LEIOGNATHI, SOLVED BY X-RAY CRYSTALLOGRAPHY.
1IBB 2001-05-09 2.1 X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT W83F
1IBF 2001-05-09 2.2 X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT V29G
1IB5 2001-05-09 2.45 X-RAY 3D STRUCTURE OF P.LEIOGNATHI CU,ZN SOD MUTANT W83Y

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
100.0 Superoxide dismutase [Cu-Zn] P00446 SODC_PHOLE