Presence of a slow dimerization equilibrium on the thermal unfolding of the 205-316 thermolysin fragment at neutral pH.


Abstract

Differential scanning calorimetry and size-exclusion chromatography have been used to characterize the dimerization and unfolding of the 205-316 C-terminal fragment of thermolysin at pH 7.5. We show that the folded fragment dimerizes at low temperature with a moderate affinity and undergoes thermal unfolding according to a N(2) <==> 2N <==> 2U model. This behavior has already been observed at acid pH, where a similar dissociation equilibrium has been found [Azuaga, A., Conejero-Lara, F., Rivas G., De Filippis, V., Fontana A., & Mateo, P. L. (1995) Biochim. Biophys. Acta 1252, 95-102]. Nevertheless, at pH 7.5 the dimerization equilibrium slows down below about 30 degrees C, with virtually no interconversion between the monomeric and the dimeric states of the fragment. We have studied the kinetics of interconversion between monomer and dimer by size-exclusion chromatography experiments and have shown that a very high energy barrier (83.8 kJ/mol at 26.5 degrees C) exists between either state. A mathematical analysis of the DSC thermograms on the basis of the proposed model has allowed us to obtain the thermodynamic characterization of the dimerization and the unfolding processes of the fragment and confirms the kinetic parameters obtained in the chromatographic experiments. The thermodynamic functions for the unfolding of the fragment are compatible with some degree of disorder in the structures of both the monomer and the dimer. According to circular dichroism measurements, the dimerization of the fragment seems to be linked to some conformational change in the subunits, most probably due to a rearrangement of the existing secondary-structure elements. This fragment displays several features already observed in folding intermediates, such as the partial disorder of the polypeptidic chain, association processes, and kinetic barriers between different regions in the conformational space. Study holds ProTherm entries: 5010 Extra Details:

Submission Details

ID: 7CKf48fN4

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:27 p.m.

Version: 1

Publication Details
Conejero-Lara F;Mateo PL,Biochemistry (1996) Presence of a slow dimerization equilibrium on the thermal unfolding of the 205-316 thermolysin fragment at neutral pH. PMID:8639498
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1TRL 1995-02-07 NMR SOLUTION STRUCTURE OF THE C-TERMINAL FRAGMENT 255-316 OF THERMOLYSIN: A DIMER FORMED BY SUBUNITS HAVING THE NATIVE STRUCTURE
5JVI 2016-12-21 1.12 Thermolysin in complex with JC148.
5N3V 2017-06-21 1.12 Thermolysin in complex with inhibitor JC292
4N4E 2014-04-02 1.13 Thermolysin in complex with UBTLN58
6GHX 2019-02-06 1.16 Alzheimer's Amyloid-Beta Peptide Fragment 31-35 in Complex with Cd-substituted Thermolysin
5JS3 2016-12-21 1.16 Thermolysin in complex with JC114.
4MZN 2014-04-02 1.17 Thermolysin in complex with UBTLN59
5ONQ 2018-08-29 1.17 Alzheimer's Amyloid-Beta Peptide Fragment 29-40 in Complex with Cd-substituted Thermolysin
5JSS 2016-12-21 1.19 Thermolysin in complex with JC149.
5FSJ 2016-10-26 1.2 Structure of thermolysin prepared by the 'soak-and-freeze' method under 45 bar of oxygen pressure
3DNZ 2009-07-07 1.2 Thermolysin by LB nanotemplate method before high X-Ray dose on ESRF ID14-2 beamline
5M9W 2017-08-16 1.21 Experimental MAD phased structure of thermolysin in complex with inhibitor JC65.
5N2X 2017-06-21 1.21 Thermolysin in complex with inhibitor JC272
3DO2 2009-07-07 1.22 Thermolysin by LB nanotemplate method after high X-Ray dose on ESRF ID14-2 beamline
5N34 2017-06-21 1.22 Thermolysin in complex with inhibitor JC276
4MWP 2014-04-02 1.23 Thermolysin in complex with UBTLN46
5L3U 2016-12-21 1.23 Thermolysin in complex with JC149 (MPD cryo protectant)
5LWD 2016-12-21 1.23 Thermolysin in complex with inhibitor (JC96)
4N5P 2014-04-02 1.25 Thermolysin in complex with UBTLN20
5MNR 2017-06-21 1.25 Thermolysin in complex with inhibitor JC256
5L41 2016-12-21 1.25 Thermolysin in complex with JC148 (MPD cryo protectant)
5LVD 2017-08-16 1.25 Thermolysin in complex with inhibitor (JC67)
5JT9 2016-12-21 1.26 Thermolysin in complex with JC106.
5A3Y 2015-11-11 1.27 SAD structure of Thermolysin obtained by multi crystal data collection
3T87 2012-08-01 1.28 Thermolysin In Complex With UBTLN28
3T74 2012-08-01 1.28 Thermolysin In Complex With UBTLN27
5L8P 2016-12-21 1.29 Thermolysin in complex with JC114 (PEG400 cryo protectant)
4OI5 2014-04-02 1.3 Glycerol-free structure of thermolysin in complex with ubtln58
5MA7 2017-08-16 1.3 Structure of thermolysin in complex with inhibitor (JC306).
5LIF 2016-12-21 1.31 Thermolysin in complex with inhibitor
4MTW 2014-04-02 1.32 Thermolysin in complex with UBTLN36
5M5F 2017-08-16 1.33 Thermolysin in complex with inhibitor and krypton
3FGD 2009-12-15 1.33 Drugscore FP: Thermoylsin in complex with fragment.
3DO1 2009-07-07 1.33 Thermolysin by Classical hanging drop method before high X-Ray dose on ESRF ID14-2 beamline
5ONP 2018-08-29 1.34 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Cd-substituted Thermolysin
5N3Y 2017-06-21 1.34 Thermolysin in complex with inhibitor JC267
5DPE 2015-10-28 1.34 Thermolysin in complex with inhibitor.
4MXJ 2014-04-02 1.35 Thermolysin in complex with UBTLN35
3DO0 2009-07-07 1.36 Thermolysin by classical hanging drop method after high X-Ray dose on esrf ID14-2 beamline
5N31 2017-06-21 1.37 Thermolysin in complex with inhibitor JC277
5N2Z 2017-06-21 1.37 Thermolysin in complex with inhibitor JC286
5JXN 2016-12-21 1.38 Thermolysin in complex with JC240.
4D9W 2013-01-16 1.38 Thermolysin In Complex With UBTLN32
5N2T 2017-06-21 1.38 Thermolysin in complex with inhibitor JC287
6FSM 2018-05-02 1.39 Crystal structure of TCE-treated Thermolysin
5ONR 2018-08-29 1.39 Alzheimer's Amyloid-Beta Peptide Fragment 1-40 in Complex with Thermolysin
3T8D 2012-08-01 1.41 Thermolysin In Complex With UBTLN31
3FVP 2010-02-09 1.41 Thermolysin inhibition
6FJ2 2018-01-31 1.43 Structure of Thermolysin solved from SAD data collected at the peak of the Zn absorption edge on ID30B
4N66 2014-04-02 1.44 Thermolysin in complex with UBTLN37
3T8F 2012-08-01 1.44 Thermolysin In Complex With UBTLN34
5M69 2017-08-16 1.44 Thermolysin in complex with inhibitor and xenon
5FXN 2016-03-16 1.45 Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the ESRF RoboDiff goniometer
3QGO 2012-01-04 1.45 Structure of Thermolysin in complex with L-Phenylalanine methylester
3T8H 2012-08-01 1.45 Thermolysin In Complex With UBTLN29
5DPF 2015-10-28 1.47 Thermolysin in complex with inhibitor.
3QH5 2012-01-04 1.5 Structure of Thermolysin in complex with N-Carbobenzyloxy-L-aspartic acid and L-Phenylalanine Methyl Ester
3T8G 2012-08-01 1.5 Thermolysin In Complex With UBTLN26
5FSS 2016-10-26 1.5 Structure of thermolysin prepared by the 'soak-and-freeze' method under 40 bar of krypton pressure
6QF3 2019-07-10 1.52 X-Ray structure of Thermolysin soaked with sodium aspartate on a silicon chip
3MS3 2011-05-11 1.54 Crystal structure of Thermolysin in complex with Aniline
3QH1 2012-01-04 1.55 Structure of Thermolysin in complex with N-benzyloxycarbonyl-L-aspartic acid
3FXS 2010-02-09 1.55 Metal exchange in thermolysin
3FV4 2010-02-09 1.56 Thermolysin inhibition
4H57 2012-10-03 1.56 Thermolysin inhibition
6TMN 1989-01-09 1.6 Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond
3FB0 2009-12-08 1.6 Metal exchange in thermolysin
8TLN 1994-04-30 1.6 STRUCTURAL COMPARISON SUGGESTS THAT THERMOLYSIN AND RELATED NEUTRAL PROTEASES UNDERGO HINGE-BENDING MOTION DURING CATALYSIS
5UN3 2017-02-22 1.6 Tetragonal thermolysin (295 K) in the presence of 50% xylose
5UU9 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 40% xylose as cryoprotectant
2TMN 1989-01-09 1.6 CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN
3T73 2012-08-01 1.6 Thermolysin In Complex With UBTLN22
5UUE 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
5UUC 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% mpd as cryoprotectant
5TMN 1989-01-09 1.6 Slow-and fast-binding inhibitors of thermolysin display different modes of binding. crystallographic analysis of extended phosphonamidate transition-state analogues
1KJP 2002-12-05 1.6 Thermolysin complexed with Z-L-Glutamic acid (benzyloxycarbonyl-L-Glutamic acid)
5UUA 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
5UUD 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 50% dmf as cryoprotectant
5UU7 2017-03-08 1.6 Tetragonal thermolysin (295 K) in the presence of 50% mpd
1KKK 2002-12-10 1.6 Thermolysin complexed with Z-L-Aspartic Acid (benzyloxycarbonyl-L-Aspartic Acid)
5UUB 2017-03-08 1.6 Tetragonal thermolysin cryocooled to 100 K with 25% xylose/25% mpd as cryoprotectant
1KEI 2002-11-16 1.6 Thermolysin (substrate-free)
1KJO 2002-12-05 1.6 Thermolysin complexed with Z-L-Threonine (benzyloxycarbonyl-L-Threonine)
2TLX 2000-03-13 1.65 THERMOLYSIN (NATIVE)
3MSA 2011-05-11 1.66 Crystal structure of Thermolysin in complex with 3-Bromophenol
3T8C 2012-08-01 1.66 Thermolysin In Complex With UBTLN30
3F28 2009-11-17 1.68 Thermolysin inhibition
1THL 1994-01-31 1.7 Thermolysin complexed with a novel glutaramide derivative, n-(1-(2(r,s)-carboxy-4-phenylbutyl) cyclopentylcarbonyl)-(s)-tryptophan
1LNF 1995-05-08 1.7 A structural analysis of metal substitutions in thermolysin
1LNE 1995-05-08 1.7 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
3TMN 1989-01-09 1.7 THE BINDING OF L-VALYL-L-TRYPTOPHAN TO CRYSTALLINE THERMOLYSIN ILLUSTRATES THE MODE OF INTERACTION OF A PRODUCT OF PEPTIDE HYDROLYSIS
4TMN 1989-01-09 1.7 SLOW-AND FAST-BINDING INHIBITORS OF THERMOLYSIN DISPLAY DIFFERENT MODES OF BINDING. CRYSTALLOGRAPHIC ANALYSIS OF EXTENDED PHOSPHONAMIDATE TRANSITION-STATE ANALOGUES
1FJQ 2001-04-18 1.7 THERMOLYSIN (70% ACETONE SOAKED CRYSTALS)
1PE5 2004-06-08 1.7 Thermolysin with tricyclic inhibitor
1HYT 1994-07-31 1.7 RE-DETERMINATION AND REFINEMENT OF THE COMPLEX OF BENZYLSUCCINIC ACID WITH THERMOLYSIN AND ITS RELATION TO THE COMPLEX WITH CARBOXYPEPTIDASE A
6FHP 2018-09-12 1.7 DAIP in complex with a C-terminal fragment of thermolysin
1LND 1995-05-08 1.7 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1KS7 2003-01-11 1.7 Thermolysin complexed with Z-D-Aspartic acid (benzyloxycarbonyl-D-Aspartic acid)
1ZDP 2005-04-26 1.7 Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (S)-thiorphan
5FSP 2016-10-26 1.7 Structure of thermolysin prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure
1KRO 2003-01-10 1.7 Thermolysin complexed with Z-D-Threonine (benzyloxycarbonyl-D-Threonine)
1Z9G 2005-04-19 1.7 Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (R)-retro-thiorphan
6QF2 2019-07-10 1.73 X-Ray structure of Thermolysin crystallized on a silicon chip
3FCQ 2009-12-08 1.75 Thermolysin inhibition
2WHZ 2010-05-12 1.75 Dipeptide Inhibitors of Thermolysin
1KL6 2002-12-11 1.8 Thermolysin complexed with Z-L-Alanine (benzyloxycarbonyl-L-Alanine)
1LNB 1995-05-08 1.8 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
1PE8 2004-06-08 1.8 Thermolysin with monocyclic inhibitor
1KR6 2003-01-09 1.8 Thermolysin complexed with Z-D-Glutamic acid (benzyloxycarbonyl-D-Glutamic acid)
1LNC 1995-05-08 1.8 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
4TNL 2014-07-09 1.8 1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL
3SSB 2011-09-07 1.8 Structure of Insect Metalloproteinase Inhibitor in Complex with Thermolysin
6IG7 2018-11-07 1.8 Crystal structure of thermolysin delivered in polyacrylamide using x-ray free electron laser
1PE7 2004-06-08 1.82 Thermolysin with bicyclic inhibitor
2A7G 2005-07-19 1.85 On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength
3N21 2011-05-25 1.87 Crystal structure of Thermolysin in complex with S-1,2-Propandiol
3EIM 2009-10-06 1.88 Metal exchange in Thermolysin
5O8N 2017-08-09 1.9 Structure of thermolysin at room temperature via a method of acoustically induced rotation.
1TMN 1989-01-09 1.9 Binding of n-carboxymethyl dipeptide inhibitors to thermolysin determined by x-ray crystallography. a novel class of transition-state analogues for zinc peptidases
4D91 2013-01-16 1.9 Thermolysin In Complex With DMSO And Acetate
1LNA 1995-05-08 1.9 A STRUCTURAL ANALYSIS OF METAL SUBSTITUTIONS IN THERMOLYSIN
5WR5 2017-08-16 1.9 Thermolysin, liganded form with cryo condition 1
1FJW 2001-04-18 1.9 THERMOLYSIN (50 MM PHENOL SOAKED)
1KTO 2003-01-17 1.9 Thermolysin complexed with Z-D-Alanine (benzyloxycarbonyl-D-Alanine)
3FBO 2009-12-08 1.92 Metal exchange in Thermolysin
3FOR 2010-01-12 1.93 Thermolysin Complexed with BNPA (2-Benzyl-3-Nitro Propanoic Acid Amide)
3F2P 2009-11-17 1.95 Thermolysin inhibition
4TLI 2000-03-13 1.95 THERMOLYSIN (25% ISOPROPANOL SOAKED CRYSTALS)
7TLI 2000-03-13 1.95 THERMOLYSIN (90% ISOPROPANOL SOAKED CRYSTALS)
2WI0 2010-05-12 1.95 Dipeptide Inhibitors of Thermolysin
3TLI 2000-02-18 1.95 THERMOLYSIN (10% ISOPROPANOL SOAKED CRYSTALS)
2TLI 2000-02-18 1.95 THERMOLYSIN (5% ISOPROPANOL SOAKED CRYSTALS)
3T2H 2011-12-28 1.95 Tetragonal thermolysin in the presence of TMAO
3MSN 2011-05-11 1.97 Crystal structure of Thermolysin in complex with N-methylurea
3FLF 2010-01-12 1.97 Thermolysin inhibition
2G4Z 2007-02-20 1.98 anomalous substructure of thermolysin
3LS7 2011-02-23 1.98 Crystal structure of Thermolysin in complex with Xenon
6D5U 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
1FJV 2001-04-18 2.0 THERMOLYSIN (60% ACETONITRILE SOAKED CRYSTALS)
1FJO 2001-04-18 2.0 THERMOLYSIN (60% ACETONE SOAKED CRYSTALS)
5WR2 2017-08-16 2.0 Thermolysin, SFX liganded form with oil-based carrier
6D5R 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
6D5N 2018-10-31 2.0 Hexagonal thermolysin (295) in the presence of 50% xylose
1QF1 1999-12-29 2.0 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYLHEPTANOYL)-PHE-ALA. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
1FJU 2001-04-18 2.0 THERMOLYSIN (80% ACETONITRILE SOAKED CRYSTALS)
5TAK 2017-08-30 2.0 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
6D5O 2018-09-19 2.0 Hexagonal thermolysin (295 K) in the presence of 50% DMF
6D5S 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
6D5Q 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
1FJ3 2001-04-18 2.0 THERMOLYSIN (50% ACETONE SOAKED)
3ZI6 2013-05-01 2.0 Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the GRob robot goniometer
3T2J 2011-12-28 2.0 Tetragonal thermolysin in the presence of betaine
6D5T 2018-09-19 2.0 Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
5TAJ 2017-08-30 2.03 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5TAC 2017-08-30 2.04 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
3FXP 2010-02-09 2.05 Thermolysin inhibition
3NN7 2011-05-25 2.05 Crystal structure of Thermolysin in complex with 2-bromoacetate
1TLI 2000-02-18 2.05 THERMOLYSIN (2% ISOPROPANOL SOAKED CRYSTALS)
1QF2 1999-12-29 2.06 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-GLY-(5-PHENYLPROLINE). PARAMETERS FOR ZN-MONODENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
3MSF 2011-05-11 2.09 Crystal structure of Thermolysin in complex with Urea
5T9I 2017-08-30 2.09 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5TAD 2017-08-30 2.09 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5WR4 2017-08-16 2.1 Thermolysin, SFX unliganded form with oil-based carrier
3T2I 2011-12-28 2.1 Tetragonal thermolysin in the presence of sarcosine
5TLI 2000-03-13 2.1 THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
1OS0 2003-03-25 2.1 Thermolysin with an alpha-amino phosphinic inhibitor
4OW3 2014-03-12 2.1 Thermolysin structure determined by free-electron laser
1TLX 2000-03-13 2.1 THERMOLYSIN (NATIVE)
5T9K 2017-08-30 2.1 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
6TLI 2000-03-13 2.1 THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)
5WR3 2017-08-16 2.1 Thermolysin, SFX liganded form with water-based carrier
5T9Q 2017-08-30 2.1 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
1Y3G 2006-01-17 2.1 Crystal Structure of a Silanediol Protease Inhibitor Bound to Thermolysin
1GXW 2002-12-05 2.18 the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate
1QF0 1999-12-29 2.2 THERMOLYSIN (E.C.3.4.24.27) COMPLEXED WITH (2-SULPHANYL-3-PHENYLPROPANOYL)-PHE-TYR. PARAMETERS FOR ZN-BIDENTATION OF MERCAPTOACYLDIPEPTIDES IN METALLOENDOPEPTIDASE
3P7V 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure C (4.8 MGy)
1FJT 2001-04-18 2.2 THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)
3P7T 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure A (0.1 MGy)
3P7S 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure D (7.2 MGy)
3P7U 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure B (2.4 MGy)
3P7P 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure A (0.1 MGy)
8TLI 2000-03-13 2.2 THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)
3P7W 2010-12-08 2.2 Radiation damage study of thermolysin - 160K structure D (7.1 MGy)
3P7Q 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure B (2.5 MGy)
3P7R 2010-12-08 2.2 Radiation damage study of thermolysin - 100K structure C (4.9 MGy)
5DLH 2015-11-18 2.25 SFX structure of thermolysin
4TLN 1982-05-26 2.3 BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS
5TAE 2017-08-30 2.3 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
1TLP 1989-01-09 2.3 CRYSTALLOGRAPHIC STRUCTURAL ANALYSIS OF PHOSPHORAMIDATES AS INHIBITORS AND TRANSITION-STATE ANALOGS OF THERMOLYSIN
7TLN 1983-03-09 2.3 STRUCTURAL ANALYSIS OF THE INHIBITION OF THERMOLYSIN BY AN ACTIVE-SITE-DIRECTED IRREVERSIBLE INHIBITOR
1L3F 2002-07-03 2.3 Thermolysin in the Absence of Substrate has an Open Conformation
5TLN 1982-05-26 2.3 BINDING OF HYDROXAMIC ACID INHIBITORS TO CRYSTALLINE THERMOLYSIN SUGGESTS A PENTACOORDINATE ZINC INTERMEDIATE IN CATALYSIS
5TAI 2017-08-30 2.3 Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin
5WR6 2017-08-16 2.3 Thermolysin, liganded form with cryo condition 2
5K7T 2017-04-05 2.5 MicroED structure of thermolysin at 2.5 A resolution
5UU8 2017-03-08 2.5 Tetragonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
6D5P 2018-09-19 3.0 Hexagonal thermolysin cryocooled to 100 K with 20% xylose as cryoprotectant

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
99.4 Thermolysin P43133 THER_GEOSE
100.0 Thermolysin P00800 THER_BACTH