Thermodynamic analysis of protein unfolding in aqueous solutions as a multisite reaction of protein with water and solute molecules.


Abstract

Thermal unfolding of ribonuclease A, lysozyme, and chymotrypsinogen A was analyzed as a multisite reaction of a protein molecule with water and solute molecules. The protein unfolding process in various solutions of sugars and denaturants was described well by the van't Hoff equation. The reciprocal form of the Wyman-Tanford equation, which describes the unfolded-to-folded protein ratio as a function of water activity, was successfully applied to obtain a good linear relationship. From this analysis, the role of water activity on protein stability was clearly explained and the contributions of hydration and solute binding to protein molecule were separately discussed in protein unfolding. General solution for the free energy of protein stability was obtained as a simple function of solute concentration. Study holds ProTherm entries: 25294, 25295, 25296, 25297, 25298, 25299, 25300, 25301, 25302, 25303, 25304, 25305, 25306, 25307, 25308, 25309, 25310, 25311, 25312, 25313, 25314, 25315, 25316, 25317, 25318, 25319, 25320, 25321, 25322, 25323, 25324, 25325, 25326, 25327, 25328, 25329, 25330, 25331, 25332, 25333, 25334, 25335, 25336, 25337, 25338, 25339, 25340, 25341, 25342, 25343, 25344, 25345, 25346, 25347, 25348, 25349, 25350, 25351, 25352, 25353, 25354, 25355, 25356, 25357, 25358, 25359, 25360, 25361, 25362, 25363, 25364, 25365, 25366, 25367, 25368, 25369, 25370, 25371, 25372, 25373, 25374, 25375, 25376, 25377, 25378, 25379, 25380, 25381, 25382, 25383, 25384, 25385, 25386, 25387, 25388, 25389, 25390, 25391, 25392, 25393, 25394, 25395, 25396, 25397, 25398, 25399, 25400, 25401, 25402, 25403, 25404, 25405, 25406, 25407, 25408, 25409 Extra Details: Thermal unfolding of protein; Multisite reaction of protein; Water activity; Hydration; Binding of solute; Free energy for protein unfolding

Submission Details

ID: 6njRLqAy

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:55 p.m.

Version: 1

Publication Details
Miyawaki O,Biophys. Chem. (2009) Thermodynamic analysis of protein unfolding in aqueous solutions as a multisite reaction of protein with water and solute molecules. PMID:19573978
Additional Information

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