Determining globular protein stability: guanidine hydrochloride denaturation of myoglobin.

Abstract

Study holds ProTherm entries: 7360, 7361, 7362, 7363, 7364 Extra Details: absorbancy measurement; free energy of denaturation;,GdnHCl binding site; binding constant

Submission Details

ID: 69NGfptK

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:33 p.m.

Version: 1

Publication Details
Pace CN;Vanderburg KE,Biochemistry (1979) Determining globular protein stability: guanidine hydrochloride denaturation of myoglobin. PMID:570408
Additional Information

Number of data points 5
Proteins Myoglobin ; Myoglobin
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dG_H2O pH:3.2 ; Experimental Assay: dG_H2O pH:6.6 ; Experimental Assay: dG_H2O pH:4.3 ; Experimental Assay: dG_H2O pH:2.9 ; Experimental Assay: dG_H2O pH:6.0
Libraries Mutations for sequence GLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQG
Sequence Assay Result Units