Determining globular protein stability: guanidine hydrochloride denaturation of myoglobin.
Abstract
Study holds ProTherm entries: 7360, 7361, 7362, 7363, 7364 Extra Details: absorbancy measurement; free energy of denaturation;,GdnHCl binding site; binding constant
Submission Details
ID: 69NGfptK
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:33 p.m.
Version: 1
Publication Details
Pace CN;Vanderburg KE,Biochemistry (1979) Determining globular protein stability: guanidine hydrochloride denaturation of myoglobin. PMID:570408Additional Information
Study Summary
| Number of data points | 5 |
| Proteins | Myoglobin ; Myoglobin |
| Unique complexes | 1 |
| Assays/Quantities/Protocols | Experimental Assay: dG_H2O pH:3.2 ; Experimental Assay: dG_H2O pH:6.6 ; Experimental Assay: dG_H2O pH:4.3 ; Experimental Assay: dG_H2O pH:2.9 ; Experimental Assay: dG_H2O pH:6.0 |
| Libraries | Mutations for sequence GLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQG |
Structure view and single mutant data analysis
Study data
| Colors: | D | E | R | H | K | S | T | N | Q | A | V | I | L | M | F | Y | W | C | G | P |
|---|
Data Distribution
Studies with similar sequences (approximate matches)
Correlation with other assays (exact sequence matches)
Relevant PDB Entries
| Structure ID | Release Date | Resolution | Structure Title |
|---|---|---|---|
| 3VM9 | 2012-09-12 | 1.05 | Dimeric horse myoglobin |
| 4TWV | 2016-05-04 | 1.06 | Horse heart myoglobin mutant (K45E/K63E/K96E) with Zn-deuteroporphyrin IX |
| 4TWU | 2016-05-04 | 1.08 | Horse heart myoglobin mutant (D44K/D60K/E85K) with Zn-deuteroporphyrin IX |
| 4NS2 | 2014-12-24 | 1.18 | Crystal structure of Horse heart ferric myoglobin; D44K/D60K/E85K mutant |
| 2FRF | 2006-05-30 | 1.2 | Horse Heart Myoglobin, Nitrite Adduct, Crystal Soak |
| 2V1F | 2007-06-12 | 1.2 | Crystal structure of radiation-induced myoglobin compound II - intermediate H at pH 8.7 |
| 2V1I | 2007-06-12 | 1.2 | Crystal structure of radiation-induced metmyoglobin - aqua ferrous myoglobin at pH 6.8 |
| 5AZR | 2016-01-20 | 1.2 | Crystal structure of aqua-cobalt(III) tetradehydrocorrin in the heme pocket of horse heart myoglobin |
| 3RJ6 | 2012-05-09 | 1.23 | Crystal Structure of Horse heart ferric myoglobin; K45E/K63E/K96E mutant |
| 6LTL | 2020-05-06 | 1.25 | The dimeric structure of G80A myoglobin |
| 2V1K | 2007-06-12 | 1.25 | Crystal structure of ferrous deoxymyoglobin at pH 6.8 |
| 2VLX | 2009-02-10 | 1.3 | Crystal structure of peroxymyoglobin generated by cryoradiolytic reduction of myoglobin compound III |
| 2FRJ | 2006-05-30 | 1.3 | Nitrosyl Horse Heart Myoglobin, Nitrite/Dithionite Method |
| 3WFT | 2014-12-03 | 1.3 | Crystal structure of horse heart myoglobin reconstituted with cobalt(II) tetradehydrocorrin |
| 2FRK | 2006-05-30 | 1.3 | Nitrosyl Horse Heart Myoglobin, Nitric Oxide Gas Method |
| 2V1H | 2007-06-12 | 1.3 | Crystal structure of radiation-induced metmyoglobin - aqua ferrous myoglobin at pH 5.2 |
| 2V1E | 2007-06-12 | 1.3 | Crystal structure of radiation-induced myoglobin compound II - intermediate H at pH 6.8 |
| 3WFU | 2014-12-03 | 1.35 | Crystal structure of horse heart myoglobin reconstituted with cobalt(I) tetradehydrocorrin |
| 1GJN | 2002-03-01 | 1.35 | Hydrogen Peroxide Derived Myoglobin Compound II at pH 5.2 |
| 2V1G | 2007-06-12 | 1.35 | Crystal structure of radiation-induced myoglobin compound II - intermediate H at pH 5.2 |
| 1DWT | 2000-03-03 | 1.4 | Photorelaxed horse heart MYOGLOBIN CO complex |
| 2V1J | 2007-06-12 | 1.4 | Crystal structure of radiation-induced metmyoglobin - aqua ferrous myoglobin at pH 8.7 |
| 5AZQ | 2016-01-20 | 1.4 | Crystal structure of cyano-cobalt(III) tetradehydrocorrin in the heme pocket of horse heart myoglobin |
| 5ZZE | 2019-02-20 | 1.42 | Crystal structure of horse myoglobin crystallized by ammonium sulfate |
| 1DWR | 2000-03-03 | 1.45 | MYOGLOBIN (HORSE HEART) WILD-TYPE COMPLEXED WITH CO |
| 1DWS | 2000-03-03 | 1.45 | PHOTOLYZED CARBONMONOXY MYOGLOBIN (HORSE HEART) |
| 4DC7 | 2013-01-23 | 1.5 | Crystal Structure of Myoglobin Exposed to Excessive SONICC Imaging Laser Dose. |
| 5YL3 | 2017-12-27 | 1.5 | Crystal structure of horse heart myoglobin reconstituted with manganese porphycene in resting state at pH 8.5 |
| 2VLZ | 2008-01-29 | 1.5 | Crystal structure of peroxymyoglobin generated by cryoradiolytic reduction of myoglobin compound III |
| 4DC8 | 2013-01-23 | 1.5 | Crystal Structure of Myoglobin Unexposed to Excessive SONICC Imaging Laser Dose. |
| 3LR9 | 2010-07-07 | 1.55 | X-ray photogenerated ferrous horse heart myoglobin, nitrite adduct |
| 5YCJ | 2018-09-19 | 1.58 | Ancestral myoglobin aMbWb' of Basilosaurus relative (polyphyly) imidazole-ligand |
| 5D5R | 2015-09-16 | 1.6 | Horse-heart myoglobin - deoxy state |
| 2O5T | 2007-10-16 | 1.6 | Cobalt horse heart myoglobin, oxidized |
| 2VM0 | 2008-01-29 | 1.6 | Crystal structure of radiation-induced myoglobin compound II generated after annealing of peroxymyoglobin |
| 2FRI | 2006-05-30 | 1.6 | Horse Heart Myoglobin, Nitrite Adduct, Co-crystallized |
| 2O5S | 2007-10-16 | 1.6 | Cobalt horse heart myoglobin, nitrite modified |
| 1NZ3 | 2003-04-08 | 1.6 | K45E-K63E Variant of Horse Heart Myoglobin |
| 2O5O | 2007-10-16 | 1.6 | Manganese horse heart myoglobin, nitrite modified |
| 2VLY | 2008-01-29 | 1.6 | Crystal structure of myoglobin compound III (radiation-induced) |
| 3LR7 | 2010-07-07 | 1.6 | Ferric horse heart myoglobin, nitrite adduct |
| 2O5M | 2007-10-16 | 1.65 | Manganese horse heart myoglobin, azide modified |
| 6LTM | 2020-05-06 | 1.65 | The dimeric structure of G80A/H81A/H82A myoglobin |
| 3V2Z | 2012-11-21 | 1.65 | Nitrite Bound Chlorin Substituted Myoglobin- Method 2 |
| 3V2V | 2012-11-21 | 1.65 | Nitrite Bound Chlorin Substituted Myoglobin- Method 1 |
| 2O58 | 2007-10-16 | 1.65 | Horse heart met manganese myoglobin |
| 2O5L | 2007-10-16 | 1.7 | Manganese horse heart myoglobin, methanol modified |
| 1WLA | 1998-01-14 | 1.7 | MYOGLOBIN (HORSE HEART) RECOMBINANT WILD-TYPE |
| 1MWC | 1998-08-19 | 1.7 | WILD TYPE MYOGLOBIN WITH CO |
| 1HRM | 1995-01-26 | 1.7 | THE PROXIMAL LIGAND VARIANT HIS93TYR OF HORSE HEART MYOGLOBIN |
| 1NZ5 | 2003-04-08 | 1.7 | The Horse heart myoglobin variant K45E/K63E complexed with Manganese |
| 1NPG | 2003-11-11 | 1.7 | MYOGLOBIN (HORSE HEART) WILD-TYPE COMPLEXED WITH NITROSOETHANE |
| 1XCH | 1997-09-17 | 1.7 | MYOGLOBIN (HORSE HEART) MUTANT WITH LEU 104 REPLACED BY ASN (L104N) |
| 3VAU | 2012-04-11 | 1.7 | Myoglobin nitrite structure: nitriheme modified |
| 1RSE | 1996-12-23 | 1.7 | MYOGLOBIN (HORSE HEART) MUTANT WITH SER 92 REPLACED BY ASP (S92D) |
| 1MYG | 1994-01-31 | 1.75 | HIGH RESOLUTION X-RAY STRUCTURES OF PIG METMYOGLOBIN AND TWO CD3 MUTANTS MB(LYS45-> ARG) AND MB(LYS45-> SER) |
| 5CN4 | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: -0.1 ps time delay |
| 5CN7 | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0.2 ps time delay |
| 5CN9 | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0.4 ps time delay |
| 5CND | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 3 ps time delay |
| 1BJE | 1998-01-28 | 1.8 | H64T VARIANT OF MYOGLOBIN (HORSE HEART) RECOMBINANT WILD-TYPE COMPLEXED WITH AZIDE |
| 5CNB | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0.5 ps time delay |
| 5CNG | 2015-09-16 | 1.8 | ultrafast dynamics in myoglobin: 150 ps time delay |
| 5CN8 | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0.3 ps time delay |
| 1M6M | 1998-08-19 | 1.8 | V68N MET MYOGLOBIN |
| 1MWD | 1998-08-19 | 1.8 | WILD TYPE DEOXY MYOGLOBIN |
| 5CN5 | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0 ps time delay |
| 5CNE | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 10 ps time delay |
| 5CMV | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: dark-state, CO-ligated structure |
| 1NZ4 | 2003-04-08 | 1.8 | The horse heart myoglobin variant K45E/K63E complexed with Cadmium |
| 5CN6 | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0.1 ps time delay |
| 5CNF | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 50 ps time delay |
| 3BA2 | 2008-11-11 | 1.8 | Cyanide bound Chlorin substituted Myoglobin |
| 5CNC | 2015-09-16 | 1.8 | Ultrafast dynamics in myoglobin: 0.6 ps time delay |
| 5Z7E | 2019-01-30 | 1.8 | Horse Heart Myoglobin Mutant - H93M |
| 5Z7F | 2019-01-30 | 1.9 | Horse Heart Myoglobin Mutant -V68A/I107Y-Sulfide Derivative |
| 1MYH | 1994-01-31 | 1.9 | HIGH RESOLUTION X-RAY STRUCTURES OF PIG METMYOGLOBIN AND TWO CD3 MUTANTS MB(LYS45-> ARG) AND MB(LYS45-> SER) |
| 3HEN | 2009-12-29 | 1.9 | Ferric Horse Heart Myoglobin; H64V/V67R Mutant |
| 1NZ2 | 2003-04-08 | 1.9 | K45E Variant of Horse Heart Myoglobin |
| 1YMB | 1994-01-31 | 1.9 | HIGH RESOLUTION STUDY OF THE THREE-DIMENSIONAL STRUCTURE OF HORSE HEART METMYOGLOBIN |
| 1HSY | 1995-02-27 | 1.9 | ORIGIN OF THE PH-DEPENDENT SPECTROSCOPIC PROPERTIES OF PENTACOORDINATE METMYOGLOBIN VARIANTS |
| 1MYJ | 1994-01-31 | 1.9 | DISTAL POLARITY IN LIGAND BINDING TO MYOGLOBIN: STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF A THREONINE68(E11) MUTANT |
| 1M6C | 1998-08-19 | 1.9 | V68N MYOGLOBIN WITH CO |
| 2O5Q | 2007-10-16 | 1.9 | Manganese horse heart myoglobin, nitric oxide modified |
| 3RJN | 2012-05-09 | 1.9 | Horse heart myoglobin: D44K/D60K mutant with zinc (II) -deuteroporphyrin dimethyl ester |
| 2NSR | 2007-11-06 | 1.9 | Nitromethane Modified Horse Heart Myoglobin |
| 1NPF | 2003-11-11 | 1.9 | MYOGLOBIN (HORSE HEART) WILD-TYPE COMPLEXED WITH NITRIC OXIDE |
| 3HEP | 2009-12-29 | 1.95 | Ferric Horse Heart Myoglobin; H64V Mutant, Nitrite Modified |
| 1MNO | 1998-08-19 | 1.95 | V68N MYOGLOBIN OXY FORM |
| 5YCI | 2018-09-19 | 1.97 | Ancestral myoglobin aMbWb' of Basilosaurus relative (polyphyly) |
| 1MDN | 1998-09-30 | 1.98 | WILD TYPE MYOGLOBIN WITH CO |
| 1MYI | 1994-01-31 | 2.0 | HIGH RESOLUTION X-RAY STRUCTURES OF PIG METMYOGLOBIN AND TWO CD3 MUTANTS MB(LYS45-> ARG) AND MB(LYS45-> SER) |
| 1YMA | 1994-01-31 | 2.0 | STRUCTURAL CHARACTERIZATION OF HEME LIGATION IN THE HIS64-->TYR VARIANT OF MYOGLOBIN |
| 1AZI | 1998-02-25 | 2.0 | MYOGLOBIN (HORSE HEART) RECOMBINANT WILD-TYPE COMPLEXED WITH AZIDE |
| 2NSS | 2007-11-06 | 2.0 | Nitrobenzene Modified Horse Heart Myoglobin |
| 3WYO | 2014-11-19 | 2.0 | Heterodimeric myoglobin formed by domain swapping |
| 2O5B | 2007-10-16 | 2.0 | Manganese horse heart myoglobin, reduced |
| 3HC9 | 2009-12-29 | 2.0 | Ferric Horse Heart Myoglobin; H64V mutant |
| 1YMC | 1994-01-31 | 2.0 | THREE-DIMENSIONAL STRUCTURE OF CYANOMET-SULFMYOGLOBIN C |
| 3HEO | 2009-12-29 | 2.0 | Ferric Horse Heart Myoglobin; H64V/V67R mutant, Nitrite Modified |
| 1MNI | 1995-04-20 | 2.07 | ALTERATION OF AXIAL COORDINATION BY PROTEIN ENGINEERING IN MYOGLOBIN. BIS-IMIDAZOLE LIGATION IN THE HIS64-->VAL(SLASH)VAL68-->HIS DOUBLE MUTANT |
| 1YCB | 1994-01-31 | 2.1 | DISTAL POCKET POLARITY IN LIGAND BINDING TO MYOGLOBIN: DEOXY AND CARBONMONOXY FORMS OF A THREONINE68 (E11) MUTANT INVESTIGATED BY X-RAY CRYSTALLOGRAPHY AND INFRARED SPECTROSCOPY |
| 2IN4 | 2006-10-31 | 2.15 | Crystal Structure of Myoglobin with Charge Neutralized Heme, ZnDMb-dme |
| 1MNJ | 1995-04-20 | 2.2 | INTERACTIONS AMONG RESIDUES CD3, E7, E10 AND E11 IN MYOGLOBINS: ATTEMPTS TO SIMULATE THE O2 AND CO BINDING PROPERTIES OF APLYSIA MYOGLOBIN |
| 1MNK | 1995-04-20 | 2.2 | INTERACTIONS AMONG RESIDUES CD3, E7, E10 AND E11 IN MYOGLOBINS: ATTEMPTS TO SIMULATE THE O2 AND CO BINDING PROPERTIES OF APLYSIA MYOGLOBIN |
| 3WI8 | 2014-03-26 | 2.2 | Crystal structure of horse heart myoglobin reconstituted with manganese porphycene |
| 1MNH | 1995-05-08 | 2.3 | INTERACTIONS AMONG RESIDUES CD3, E7, E10 AND E11 IN MYOGLOBINS: ATTEMPTS TO SIMULATE THE O2 AND CO BINDING PROPERTIES OF APLYSIA MYOGLOBIN |
| 6LS8 | 2020-05-06 | 2.3 | The monomeric structure of G80A/H81A/H82A myoglobin |
| 5YCG | 2018-09-19 | 2.4 | Ancestral myoglobin aMbWp of Pakicetus relative |
| 1PMB | 1990-01-15 | 2.5 | THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT |
| 1YCA | 1994-01-31 | 2.9 | DISTAL POCKET POLARITY IN LIGAND BINDING TO MYOGLOBIN: DEOXY AND CARBONMONOXY FORMS OF A THREONINE68 (E11) MUTANT INVESTIGATED BY X-RAY CRYSTALLOGRAPHY AND INFRARED SPECTROSCOPY |
Relevant UniProtKB Entries
| Percent Identity | Matching Chains | Protein | Accession | Entry Name |
|---|---|---|---|---|
| 90.3 | Myoglobin | P02163 | MYG_ROUAE | |
| 90.9 | Myoglobin | P11343 | MYG_LUTLU | |
| 90.3 | Myoglobin | P02165 | MYG_TUPGL | |
| 90.3 | Myoglobin | P02167 | MYG_NYCCO | |
| 90.3 | Myoglobin | Q0KIY0 | MYG_MESST | |
| 90.3 | Myoglobin | P02183 | MYG_MESCA | |
| 90.8 | Myoglobin | P02177 | MYG_ESCRO | |
| 90.8 | Myoglobin | Q0KIY2 | MYG_BALED | |
| 90.8 | Myoglobin | Q0KIY1 | MYG_BALBO | |
| 90.9 | Myoglobin | P02189 | MYG_PIG | |
| 90.9 | Myoglobin | P02166 | MYG_PERPO | |
| 92.2 | Myoglobin | P02169 | MYG_LEPMU | |
| 91.6 | Myoglobin | P02181 | MYG_INIGE | |
| 100.0 | Myoglobin | P68082 | MYG_HORSE | |
| 100.0 | Myoglobin | P68083 | MYG_EQUBU | |
| 90.1 | Myoglobin | P02178 | MYG_MEGNO |