Thermal stability and aggregation of creatine kinase from rabbit skeletal muscle. Effect of 2-hydroxypropyl-beta-cyclodextrin.


Abstract

Effect of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on thermal aggregation of creatine kinase from rabbit skeletal muscle (RMCK) at 48 degrees C has been studied using dynamic light scattering. An increase in the duration of the lag period on the kinetic curves of aggregation, registered as an increment of the light scattering intensity in time, has been observed in the presence of HP-beta-CD. It has been shown that the initial parts of the dependences of the hydrodynamic radius (R(h)) of the protein aggregates on time follow the exponential law. The reciprocal value of parameter t(2R) (t(2R) is the time interval over which the R(h) value is doubled) was used to characterize the rate of aggregation. A 10-fold decrease in the 1/t(2R) value was observed in the presence of 76mM HP-beta-CD. Judging from the data on the kinetics of RMCK inactivation and the data on differential scanning calorimetry of RMCK, HP-beta-CD does not affect the rate of RMCK unfolding. Study holds ProTherm entries: 25812, 25813, 25814, 25815 Extra Details: Creatine kinase; 2-Hydroxypropyl-cyclodextrin; Inactivation; Denaturation; Aggregation

Submission Details

ID: 63tvQ5KJ3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:56 p.m.

Version: 1

Publication Details
Maloletkina OI;Markossian KA;Belousova LV;Kleimenov SY;Orlov VN;Makeeva VF;Kurganov BI,Biophys. Chem. (2010) Thermal stability and aggregation of creatine kinase from rabbit skeletal muscle. Effect of 2-hydroxypropyl-beta-cyclodextrin. PMID:20378240
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1U6R 2005-08-02 1.65 Transition state analog complex of muscle creatine kinase (R134K) mutant
1G0W 2001-02-07 2.3 CRYSTAL STRUCTURE OF BOVINE RETINAL CREATINE KINASE
2CRK 1999-01-13 2.35 MUSCLE CREATINE KINASE
1I0E 2003-04-01 3.5 CRYSTAL STRUCTURE OF CREATINE KINASE FROM HUMAN MUSCLE

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
91.3 Creatine kinase M-type P00565 KCRM_CHICK
96.6 Creatine kinase M-type P06732 KCRM_HUMAN
96.9 Creatine kinase M-type P05123 KCRM_CANLF
97.4 Creatine kinase M-type Q9XSC6 KCRM_BOVIN
97.4 Creatine kinase M-type Q5XLD3 KCRM_PIG
97.9 Creatine kinase M-type P00564 KCRM_RAT
97.9 Creatine kinase M-type P07310 KCRM_MOUSE
100.0 Creatine kinase M-type P00563 KCRM_RABIT