The N-terminal region is crucial for the thermostability of the G-domain of Bacillus stearothermophilus EF-Tu.


Abstract

Bacterial elongation factor Tu (EF-Tu) is a model monomeric G protein composed of three covalently linked domains. Previously, we evaluated the contributions of individual domains to the thermostability of EF-Tu from the thermophilic bacterium Bacillus stearothermophilus. We showed that domain 1 (G-domain) sets up the basal level of thermostability for the whole protein. Here we chose to locate the thermostability determinants distinguishing the thermophilic domain 1 from a mesophilic domain 1. By an approach of systematically swapping protein regions differing between G-domains from mesophilic Bacillus subtilis and thermophilic B. stearothermophilus, we demonstrate that a small portion of the protein, the N-terminal 12 amino acid residues, plays a key role in the thermostability of this domain. We suggest that the thermostabilizing effect of the N-terminal region could be mediated by stabilizing the functionally important effector region. Finally, we demonstrate that the effect of the N-terminal region is significant also for the thermostability of the full-length EF-Tu. Study holds ProTherm entries: 25745, 25746, 25747, 25748, 25749, 25750, 25751, 25752, 25753, 25754, 25755, 25756, 25757, 25758, 25759, 25760, 25761, 25762, 25763, 25764, 25765, 25766, 25767, 25768, 25769, 25770 Extra Details: G domain; EF-Tu in GDP form Thermostability; G protein; EF-Tu; G-domain; Bacillus

Submission Details

ID: 5BcYwWAP

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:56 p.m.

Version: 1

Publication Details
Sanderová H;Tiserová H;Barvík I;Sojka L;Jonák J;Krásný L,Biochim. Biophys. Acta (2010) The N-terminal region is crucial for the thermostability of the G-domain of Bacillus stearothermophilus EF-Tu. PMID:19800034
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
96.7 Elongation factor Tu B7GJ65 EFTU_ANOFW
96.5 Elongation factor Tu C5D3R5 EFTU_GEOSW
98.2 Elongation factor Tu A4IJI7 EFTU_GEOTN
99.2 Elongation factor Tu Q5L3Z9 EFTU_GEOKA
100.0 Elongation factor Tu O50306 EFTU_GEOSE