Differential scanning calorimetric studies on bovine serum albumin: III. Effect of sodium dodecyl sulphate.


Abstract

Measurements of differential scanning calorimetry (d.s.c.) have been made on the complex bovine serum albumin (BSA)--sodium dodecyl sulphate (SDS) under various conditions. There are two peaks P1 and P2 in the d.s.c. curve for BSA at pH 7 and in the absence of NaCl, indicating the presence of the heat-induced transition of BSA. There are three peaks P1, P2 and P3 in the curve for the system with the molar mixing ratio SDS/BSA = 1. With the increase in the amount of SDS, the peak P3 grows at the expense of P1 and P2. There is only a single peak P3 in the curve for the systems SDS/BSA > 7, and no peak at SDS/BSA = 50 and 100. There is a single peak P12 in the curve for BSA at pH 7 and in the presence of 0.05 M NaCl, indicating that the heat-induced transition is suppressed. There are two peaks P12 and P3 for the systems SDS/BSA = 1-5; the area ratio of the peak P3 to P12 increases with the increase in the amount of SDS. There is only a single peak P3 when SDS/BSA > 7, and no peak at SDS/BSA = 50. It is concluded that the peak P3 is a product of SDS regardless of the presence or absence of NaCl. Values of thermal denaturation temperature (Td) and enthalpy (delta H) of thermal denaturation indicate that the complex AD12 (A = BSA, D = SDS) is in the most thermostabilized state.(ABSTRACT TRUNCATED AT 250 WORDS) Study holds ProTherm entries: 10110 Extra Details: thermal denaturation; bovine serum albumin;,differential scanning calorimetry;sodium dodecyl sulphate

Submission Details

ID: 3xJZBcQA3

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:39 p.m.

Version: 1

Publication Details
Yamasaki M;Yano H;Aoki K,Int. J. Biol. Macromol. (1992) Differential scanning calorimetric studies on bovine serum albumin: III. Effect of sodium dodecyl sulphate. PMID:1476985
Additional Information

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
2L7U 2011-05-18 Structure of CEL-PEP-RAGE V domain complex
4LUH 2014-09-17 2.2 Complex of ovine serum albumin with 3,5-diiodosalicylic acid
4LUF 2014-09-17 2.3 Crystal Structure of Ovine Serum Albumin
4F5S 2012-10-03 2.47 Crystal Structure of Bovine Serum Albumin
5ORF 2017-11-15 2.54 Structure of ovine serum albumin in P1 space group
4OR0 2014-06-04 2.58 Crystal Structure of Bovine Serum Albumin in complex with naproxen
4JK4 2013-07-24 2.65 Crystal Structure of Bovine Serum Albumin in complex with 3,5-diiodosalicylic acid
3V03 2012-01-04 2.7 Crystal structure of Bovine Serum Albumin
6RJV 2019-08-14 3.21 The X-ray structure of the Gold/Serum Albumin adduct obtained upon reaction of the protein with AuL12, a gold(III) dithiocarbamate complex

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
92.4 Serum albumin P14639 ALBU_SHEEP
100.0 Serum albumin P02769 ALBU_BOVIN