A differential scanning calorimetric study of the binding of sulfate ion and of Cibacron blue F3GA to yeast phosphoglycerate kinase.


Abstract

In continuation of earlier work [Hu, C. Q., & Sturtevant, J.M. (1987) Biochemistry 26, 178-182], differential scanning calorimetry has been employed in a study of the effects on the thermal denaturation of yeast phosphoglycerate kinase of two inhibitors of the enzyme, sulfate ion and the dye Cibracron blue F3GA. Sulfate ion, as is usual with ligands that dissociate during unfolding of the host protein, raises t1/2, the temperature of half-completion of the denaturation, has only a modest effect, stemming from the enthalpy of dissociation of the ligand, on the enthalpy of denaturation, and has little or no effect on the heat capacity change resulting from denaturation. In sharp contrast, Cibacron blue F3GA lowers t1/2 and drastically decreases both the enthalpy and heat capacity changes due to denaturation. The DSC results with sulfate ion are consistent with previous kinetic data [Scopes, R. K. (1978) Eur. J. Biochem. 91, 119-129; Khamis, M. H., & Larsson-Raznikiewicz, M. (1981) Biochim. Biophys. Acta 657, 190-194], which indicate two binding sites for sulfate ion at one of which the ligand acts as a competitive inhibitor. The results with Cibacron blue F3GA indicate that the dye induces a major destabilizing structural change in the enzyme in addition to rendering it enzymically inactive. Study holds ProTherm entries: 3836, 10723, 10724, 10725, 10726, 10727, 10728, 10729, 10730, 10731, 10732, 10733, 10734, 10735, 10736, 10737, 10738, 10739, 10740, 10741, 10742, 10743, 10744, 10745, 10746, 10747, 10748, 10749, 10750, 10751, 10752, 10753, 10754, 10755, 10756, 10757, 10758, 10759, 10760, 10761, 10762, 10763, 10764, 10765, 10766, 10767, 10768, 10769, 10770, 10771, 10772, 10773, 10774, 10775, 10776, 10777, 10778, 10779, 10780, 10781, 10782, 10783, 10784, 10785, 10786, 10787, 10788 Extra Details: enthalpy of denaturation; heat capacity changes; structural change;,enzymically inactive

Submission Details

ID: 3hkdVH5X

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:22 p.m.

Version: 1

Publication Details
Hu CQ;Sturtevant JM,Biochemistry (1989) A differential scanning calorimetric study of the binding of sulfate ion and of Cibacron blue F3GA to yeast phosphoglycerate kinase. PMID:2653429
Additional Information

Study Summary

Number of data points 268
Proteins Phosphoglycerate kinase ; Phosphoglycerate kinase
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (24.0 mM),, ionic:SO4(2- ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (24.0 mM),, ionic:SO4( ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (24.0 mM),, ionic:SO4(2-) ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (24.0 mM),, ionic:SO4(2 ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:PI ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 60.0 mM, buffers: ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:PIP ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:P ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (18.0 mM),, ionic:SO4(2- ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (18.0 mM),, ionic:SO4( ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (18.0 mM),, ionic:SO4(2-) ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (18.0 mM),, ionic:SO4(2 ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:PI ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers: ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:PIP ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:P ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (10.0 mM),, ionic:SO4(2- ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (10.0 mM),, ionic:SO4( ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (10.0 mM),, ionic:SO4(2-) ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (10.0 mM),, ionic:SO4(2 ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (4.0 mM),, ionic:SO4(2-) ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (4.0 mM),, ionic:SO4(2 ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (4.0 mM),, ionic:SO4(2-): ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (4.0 mM),, ionic:SO4(2- ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (2.0 mM),, ionic:SO4(2-) ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (2.0 mM),, ionic:SO4(2 ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (2.0 mM),, ionic:SO4(2-): ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, details:Additives 3-pG (2.0 mM),, ionic:SO4(2- ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 10.0 mM, buffers:PI ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 10.0 mM, buffers: ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 10.0 mM, buffers:PIP ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (20.0 mM),, ionic:SO4(2-): 10.0 mM, buffers:P ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 10.0 mM, buffers:PI ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 10.0 mM, buffers: ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 10.0 mM, buffers:PIP ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, details:Additives 3-pG (14.0 mM),, ionic:SO4(2-): 10.0 mM, buffers:P ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.43-1.66 mg/mL, buffers:PIPES: 10 mM, ionic:SO4(2-): 10.0 mM, details:Additives 3-pG ; Experimental Assay: dCp details:Additives MgATP (18.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2 ; Experimental Assay: dHcal details:Additives MgATP (18.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4 ; Experimental Assay: Tm details:Additives MgATP (18.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2- ; Experimental Assay: dHvH details:Additives MgATP (18.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4( ; Experimental Assay: dCp prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:P ; Experimental Assay: dHcal prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers ; Experimental Assay: Tm prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers:PI ; Experimental Assay: dHvH prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (14.0 mM),, ionic:SO4(2-): 60.0 mM, buffers: ; Experimental Assay: dCp details:Additives MgATP (10.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2 ; Experimental Assay: dHcal details:Additives MgATP (10.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4 ; Experimental Assay: Tm details:Additives MgATP (10.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2- ; Experimental Assay: dHvH details:Additives MgATP (10.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4( ; Experimental Assay: dCp prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (7.5 mM),, ionic:SO4(2-): 60.0 mM, buffers:PI ; Experimental Assay: dHcal prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (7.5 mM),, ionic:SO4(2-): 60.0 mM, buffers: ; Experimental Assay: Tm prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (7.5 mM),, ionic:SO4(2-): 60.0 mM, buffers:PIP ; Experimental Assay: dHvH prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (7.5 mM),, ionic:SO4(2-): 60.0 mM, buffers:P ; Experimental Assay: dCp buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 60.0 mM, details:Additives MgATP ; Experimental Assay: dHcal buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 60.0 mM, details:Additives MgA ; Experimental Assay: Tm buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 60.0 mM, details:Additives MgATP ; Experimental Assay: dHvH buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 60.0 mM, details:Additives MgAT ; Experimental Assay: dCp buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (3.0 mM),, ionic:SO4(2- ; Experimental Assay: dHcal buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (3.0 mM),, ionic:SO4( ; Experimental Assay: Tm buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (3.0 mM),, ionic:SO4(2-) ; Experimental Assay: dHvH buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, details:Additives MgATP (3.0 mM),, ionic:SO4(2 ; Experimental Assay: dCp details:Additives MgATP (2.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2- ; Experimental Assay: dHcal details:Additives MgATP (2.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4( ; Experimental Assay: Tm details:Additives MgATP (2.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-) ; Experimental Assay: dHvH details:Additives MgATP (2.0 mM),, buffers:PIPES: 10 mM, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2 ; Experimental Assay: dCp buffers:PIPES: 10 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2- ; Experimental Assay: dHcal buffers:PIPES: 10 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, ionic:SO4( ; Experimental Assay: Tm buffers:PIPES: 10 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-) ; Experimental Assay: dHvH buffers:PIPES: 10 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, ionic:SO4(2 ; Experimental Assay: dCp details:Additives MgATP (18.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:P ; Experimental Assay: dHcal details:Additives MgATP (18.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers ; Experimental Assay: Tm details:Additives MgATP (18.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PI ; Experimental Assay: dHvH details:Additives MgATP (18.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers: ; Experimental Assay: dCp prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (14.0 mM),, buffers:P ; Experimental Assay: dHcal prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (14.0 mM),, buffers ; Experimental Assay: Tm prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (14.0 mM),, buffers:PI ; Experimental Assay: dHvH prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (14.0 mM),, buffers: ; Experimental Assay: dCp details:Additives MgATP (10.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:P ; Experimental Assay: dHcal details:Additives MgATP (10.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers ; Experimental Assay: Tm details:Additives MgATP (10.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PI ; Experimental Assay: dHvH details:Additives MgATP (10.0 mM),, ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers: ; Experimental Assay: dCp prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (7.5 mM),, buffers:PI ; Experimental Assay: dHcal prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (7.5 mM),, buffers: ; Experimental Assay: Tm prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (7.5 mM),, buffers:PIP ; Experimental Assay: dHvH prot_conc:1.43-1.71 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (7.5 mM),, buffers:P ; Experimental Assay: dCp ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgATP ; Experimental Assay: dHcal ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgA ; Experimental Assay: Tm ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgATP ; Experimental Assay: dHvH ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgAT ; Experimental Assay: dCp ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgATP ; Experimental Assay: dHcal ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgA ; Experimental Assay: Tm ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgATP ; Experimental Assay: dHvH ionic:SO4(2-): 10.0 mM, prot_conc:1.43-1.71 mg/mL, buffers:PIPES: 50 mM, details:Additives MgAT ; Experimental Assay: dCp ionic:SO4(2-): 10.0 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, buffers:PI ; Experimental Assay: dHcal ionic:SO4(2-): 10.0 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, buffers: ; Experimental Assay: Tm ionic:SO4(2-): 10.0 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, buffers:PIP ; Experimental Assay: dHvH ionic:SO4(2-): 10.0 mM, details:Additives MgATP (1.0 mM),, prot_conc:1.43-1.71 mg/mL, buffers:P ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, ionic:SO4(2-): 60.0 mM, details:Additives 3-pG ; Experimental Assay: dCp ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dHcal ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, details:Additives 3-p ; Experimental Assay: Tm ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, buffers:PIPES: 50 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, buffers:PIPES: 50 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, buffers:PIPES: 50 mM, details:Additives 3-p ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, details:Additives 3-pG (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-): ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, ionic:SO4(2-): 8.0 mM, details:Additives 3-pG ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, buffers:PIPES: 50 mM, ionic:SO4(2-): 8.0 mM, details:Additives 3-pG (1 ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, details:Additives 3-pG (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-) ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 4.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 4.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 4.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG (1 ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 4.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ( ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG (1 ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, details:Additives 3-pG ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives MgATP ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives MgA ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives MgATP ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 80.0 mM, buffers:PIPES: 50 mM, details:Additives MgAT ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-) ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2 ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-): ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2- ; Experimental Assay: dCp ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIP ; Experimental Assay: dHcal ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:P ; Experimental Assay: Tm ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPE ; Experimental Assay: dHvH ionic:SO4(2-): 40.0 mM, prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PI ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, details:Additives MgATP (10 mM),, buffers:PIP ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, details:Additives MgATP (10 mM),, buffers:P ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, details:Additives MgATP (10 mM),, buffers:PIPE ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 20.0 mM, details:Additives MgATP (10 mM),, buffers:PI ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, details:Additives MgATP (10 mM),, buffers:PIP ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, details:Additives MgATP (10 mM),, buffers:P ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, details:Additives MgATP (10 mM),, buffers:PIPE ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 15.0 mM, details:Additives MgATP (10 mM),, buffers:PI ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (10 mM),, buffers:PIP ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (10 mM),, buffers:P ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (10 mM),, buffers:PIPE ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 10.0 mM, details:Additives MgATP (10 mM),, buffers:PI ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-) ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2 ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-): ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2- ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-) ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2 ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2-): ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, details:Additives MgATP (10 mM),, buffers:PIPES: 50 mM, ionic:SO4(2- ; Experimental Assay: dCp prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, details:Additives MgATP (10 mM),, buffers:PIPE ; Experimental Assay: dHcal prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, details:Additives MgATP (10 mM),, buffers:PI ; Experimental Assay: Tm prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, details:Additives MgATP (10 mM),, buffers:PIPES ; Experimental Assay: dHvH prot_conc:1.40-1.60 mg/mL, ionic:SO4(2-): 2.0 mM, details:Additives MgATP (10 mM),, buffers:PIP ; Experimental Assay: dCp ionic:SO4(2-): 120.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 120.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 120.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 120.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 100.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 100.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 100.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 100.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 90.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 90.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 90.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 90.0 mM, prot_conc:1.1-1.8 mg/mL, buffers:PIPES: 50 mM ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 75.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 75.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 75.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 75.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp ionic:SO4(2-): 60.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 60.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 60.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 60.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL, ionic:SO4(2-): 50.0 mM ; Experimental Assay: dHcal details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL, ionic:SO4(2-): 50.0 mM ; Experimental Assay: Tm details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL, ionic:SO4(2-): 50.0 mM ; Experimental Assay: dHvH details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL, ionic:SO4(2-): 50.0 mM ; Experimental Assay: dCp ionic:SO4(2-): 40.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 40.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 40.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 40.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 25.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 25.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 25.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 25.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp ionic:SO4(2-): 18.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 18.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 18.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 18.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp ionic:SO4(2-): 15.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 15.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 15.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 15.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp ionic:SO4(2-): 10.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 10.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 10.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 10.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp ionic:SO4(2-): 7.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 7.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 7.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 7.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 5.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 5.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 5.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 5.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 3.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 3.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 3.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 3.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp details:Additives , ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal details:Additives , ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm details:Additives , ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH details:Additives , ionic:SO4(2-): 2.0 mM, buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp ionic:SO4(2-): 1.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHcal ionic:SO4(2-): 1.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: Tm ionic:SO4(2-): 1.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dHvH ionic:SO4(2-): 1.0 mM, details:Additives , buffers:PIPES: 50 mM, prot_conc:1.1-1.8 mg/mL ; Experimental Assay: dCp prot_conc:0.6-5.4 mg/ml, details:Additives , ionic:: , buffers:PIPES: 50 mM ; Experimental Assay: dHcal prot_conc:0.6-5.4 mg/ml, details:Additives , ionic:: , buffers:PIPES: 50 mM ; Experimental Assay: Tm prot_conc:0.6-5.4 mg/ml, details:Additives , ionic:: , buffers:PIPES: 50 mM ; Experimental Assay: dHvH prot_conc:0.6-5.4 mg/ml, details:Additives , ionic:: , buffers:PIPES: 50 mM
Libraries Mutations for sequence SLSSKLSVQDLDLKDKRVFIRVDFNVPLDGKKITSNQRIVAALPTIKYVLEHHPRYVVLASHLGRPNGERNEKYSLAPVAKELQSLLGKDVTFLNDCVGPEVEAAVKASAPGSVILLENLRYHIEEEGSRKVDGQKVKASKEDVQKFRHELSSLADVYINDAFGTAHRAHSSMVGFDLPQRAAGFLLEKELKYFGKALENPTRPFLAILGGAKVADKIQLIDNLLDKVDSIIIGGGMAFTFKKVLENTEIGDSIFDKAVGPEIAKLMEKAKAKGVEVVLPVDFIIADAFSASANTKTVTDKEGIPAGWQGLDNGPESRKLFAATVAKATVILWNGPPGVFEFEKFAAGTKALLDEVVKSSAAGNTVIIGGGDTATVAKKYGVTDKISHVSTGGGASLELLEGKELPGVAFLSEKK

Structure view and single mutant data analysis

Study data

No weblogo for data of varying length.
Colors: D E R H K S T N Q A V I L M F Y W C G P
 

Data Distribution

Studies with similar sequences (approximate matches)

Correlation with other assays (exact sequence matches)


Relevant PDB Entries

Structure ID Release Date Resolution Structure Title
1FW8 2001-03-22 2.3 CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72
1QPG 1996-06-10 2.4 3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q
3PGK 1982-09-24 2.5 The structure of yeast phosphoglycerate kinase at 0.25 nm resolution

Relevant UniProtKB Entries

Percent Identity Matching Chains Protein Accession Entry Name
94.5 Phosphoglycerate kinase Q6FKY1 PGK_CANGA
100.0 Phosphoglycerate kinase P00560 PGK_YEAST