Thermal denaturation of erythrocyte carbonic anhydrase.
Abstract
An experimental study on the thermal behaviour of erythrocyte carbonic anhydrase was carried out with the main aim to estimate the thermodynamic parameters that control the stability of the enzyme. The effects of thermal denaturation on the catalytic properties of the enzyme were also investigated. Below 60 degrees C the enzyme was found to be very stable, whereas between 60 and 65 degrees C a drastic decrease in the biological activity was observed. From the obtained results some considerations were made about the stabilization of the active form of the protein. Study holds ProTherm entries: 10121, 10122 Extra Details: carbonic anhydrase; thermal denaturation; conformational stability;,bovine erythrocytes
Submission Details
ID: 35X8xCNJ4
Submitter: Connie Wang
Submission Date: April 24, 2018, 8:39 p.m.
Version: 1
Publication Details
Lavecchia R;Zugaro M,FEBS Lett. (1991) Thermal denaturation of erythrocyte carbonic anhydrase. PMID:1959599Additional Information
Study Summary
Structure view and single mutant data analysis
Study data
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Data Distribution
Studies with similar sequences (approximate matches)