Thermodynamics of ubiquitin unfolding.


Abstract

The energetics of ubiquitin unfolding have been studied using differential scanning microcalorimetry. For the first time it has been shown directly that the enthalpy of protein unfolding is a nonlinear function of temperature. Thermodynamic parameters of ubiquitin unfolding were correlated with the structure of the protein. The enthalpy of hydrogen bonding in ubiquitin was calculated and compared to that obtained for other proteins. It appears that the energy of hydrogen bonding correlates with the average length of the hydrogen bond in a given protein structure. Study holds ProTherm entries: 2419, 2420, 2421, 2422, 2423, 2424, 2425, 2426 Extra Details: microcalorimetry; heat capacity; enthalpy; hydrogen bonding

Submission Details

ID: 2W2ZqP6h

Submitter: Connie Wang

Submission Date: April 24, 2018, 8:18 p.m.

Version: 1

Publication Details
Wintrode PL;Makhatadze GI;Privalov PL,Proteins (1994) Thermodynamics of ubiquitin unfolding. PMID:8202465
Additional Information

Number of data points 24
Proteins Polyubiquitin-C
Unique complexes 1
Assays/Quantities/Protocols Experimental Assay: dHcal buffers:Sodium acetate-Acetic acid: 10 mM, pH:4.0 ; Experimental Assay: Tm buffers:Sodium acetate-Acetic acid: 10 mM, pH:4.0 ; Experimental Assay: dHvH buffers:Sodium acetate-Acetic acid: 10 mM, pH:4.0 ; Experimental Assay: dHcal buffers:Sodium acetate-Acetic acid: 10 mM, pH:3.5 ; Experimental Assay: Tm buffers:Sodium acetate-Acetic acid: 10 mM, pH:3.5 ; Experimental Assay: dHvH buffers:Sodium acetate-Acetic acid: 10 mM, pH:3.5 ; Experimental Assay: dHcal pH:3.25, buffers:glycine-HCl: 10 mM ; Experimental Assay: Tm pH:3.25, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHvH pH:3.25, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHcal pH:3.0, buffers:glycine-HCl: 10 mM ; Experimental Assay: Tm pH:3.0, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHvH pH:3.0, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHcal pH:2.75, buffers:glycine-HCl: 10 mM ; Experimental Assay: Tm pH:2.75, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHvH pH:2.75, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHcal pH:2.5, buffers:glycine-HCl: 10 mM ; Experimental Assay: Tm pH:2.5, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHvH pH:2.5, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHcal pH:2.2, buffers:glycine-HCl: 10 mM ; Experimental Assay: Tm pH:2.2, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHvH pH:2.2, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHcal pH:2.0, buffers:glycine-HCl: 10 mM ; Experimental Assay: Tm pH:2.0, buffers:glycine-HCl: 10 mM ; Experimental Assay: dHvH pH:2.0, buffers:glycine-HCl: 10 mM
Libraries Mutations for sequence MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Sequence Assay Result Units