Apparent Kinetic Parameters of E. coli BCAT and Its Mutants for Transamination of Various Amino-Acid Donors with α-Ketoglutarate as the Acceptor

MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIFVGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEGRCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ

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WTnumMUT

File Description

Column Assay/Protocol
1 Sequence Description
2 Experimental Assay: Km α-Ketoglutarate
3 Experimental Assay: Ki α-Ketoglutarate
4 Experimental Assay: kcat α-Ketoglutarate
5 Experimental Assay: kcat/Km α-Ketoglutarate
6 Experimental Assay: Km L-Histidine
7 Experimental Assay: kcat L-Histidine
8 Experimental Assay: kcat/Km L-Histidine
9 Experimental Assay: Km L-Leucine
10 Experimental Assay: kcat L-Leucine
11 Experimental Assay: kcat/Km L-Leucine
12 Experimental Assay: Km L-Threonine
13 Experimental Assay: kcat L-Threonine
14 Experimental Assay: kcat/Km L-Threonine