in vivo chimeric screen of saturation mutagenesis lib compared to GMEC from CPD at positions 23, 27, 45

MTYKLILNGKTLKGETTTEAVDIATAADVFAQYAADNGVKGEWTADEATKTFTVTE

23,27,45

1

MutatedResRange

File Description

Column Assay/Protocol
3 Experimental Assay: Tm
4 Experimental Assay: Bacterial growth in 3000 ug/ml carbenicillin (antibiotic)
5 Derived Quantity: SEM of growth (OD600)
6 Computational Protocol: ORBIT total energy