random mutagenesis (#1-5) and CPD of MonB (MonB-ORDES) at pos 23, 27, 45 to validate utility of chimeric screen (Table 3 Tm and OD)
MTYKLILNGKTLKGETTTEAVDIATAADVFAQYAADNGVKGEWTADEATKTFTVTE
23,27,45
1
MutatedResRange
Column | Assay/Protocol |
---|---|
1 | Experimental Assay: Tm |
2 | Experimental Assay: Bacterial growth in 3000 ug/ml carbenicillin (antibiotic) |
3 | Derived Quantity: SEM of growth (OD600) |
4 | Label |