random mutagenesis (#1-5) and CPD of MonB (MonB-ORDES) at pos 23, 27, 45 to validate utility of chimeric screen (Table 3 Tm and OD)

MTYKLILNGKTLKGETTTEAVDIATAADVFAQYAADNGVKGEWTADEATKTFTVTE

23,27,45

1

MutatedResRange

File Description

Column Assay/Protocol
1 Experimental Assay: Tm
2 Experimental Assay: Bacterial growth in 3000 ug/ml carbenicillin (antibiotic)
3 Derived Quantity: SEM of growth (OD600)
4 Label