GB1 mutants with variable stability for use in testing in vivo chimeric screen (Table 1 Tm + Fig 4 OD + Table 2 Proteolysis)
MTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTE
1
WTnumMUT
| Column | Assay/Protocol |
|---|---|
| 1 | Experimental Assay: Tm |
| 2 | Experimental Assay: Bacterial growth in 3000 ug/ml carbenicillin (antibiotic) |
| 3 | Derived Quantity: SEM of growth (OD600) |
| 4 | Experimental Assay: in vitro proteolysis |
| 5 | Label |